BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147007.6 - phase: 0 /pseudo
(1105 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV406379 112 3e-25
BP063694 94 9e-20
AU088980 88 8e-18
BP033602 80 2e-15
AV766088 75 6e-14
TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragm... 71 1e-12
BP054557 55 8e-08
AV769206 53 2e-07
AV427921 52 4e-07
AV764934 48 7e-06
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 43 2e-04
BP054351 39 0.004
BP053302 39 0.006
BP041939 39 0.006
BP061842 38 0.008
BP063902 38 0.008
TC17551 weakly similar to UP|AAR24662 (AAR24662) At5g26960, part... 35 0.085
AV766665 35 0.085
TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II ... 34 0.11
BP081717 34 0.11
>AV406379
Length = 226
Score = 112 bits (280), Expect = 3e-25
Identities = 47/74 (63%), Positives = 62/74 (83%)
Frame = -3
Query: 146 LYYLHSHPSTICGVSASPDIIHCRLGHPSLDKLKVLVPHLSHLKSLDCESCQLGKHVRVS 205
LYY+ H ST+C + SPD++HCRLGHPSL++LK +VP LSHLK+L+CESCQLGKHVR S
Sbjct: 224 LYYMQPHLSTLCAIFESPDLLHCRLGHPSLERLKQMVPKLSHLKTLECESCQLGKHVRAS 45
Query: 206 FPSRANKRSMSPFN 219
FPS ++ ++SPF+
Sbjct: 44 FPSSTSRPAVSPFH 3
>BP063694
Length = 511
Score = 94.4 bits (233), Expect = 9e-20
Identities = 60/151 (39%), Positives = 84/151 (54%), Gaps = 11/151 (7%)
Frame = -2
Query: 121 FSSDSFLIQDRSTGKTIGT*FES*GLYYLHSHPSTICGVS-----ASPDIIHCRLGHPSL 175
F DSF+IQ+R TG +G GLY L+ + S AS ++ H RLGH +
Sbjct: 507 FLDDSFVIQNRKTGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNF 328
Query: 176 DKLKVLVPH----LSHL--KSLDCESCQLGKHVRVSFPSRANKRSMSPFNIIHSDVWGPS 229
D +K L H +S + K + C SCQ+ K R+ F NKR+ + ++IH D+ GPS
Sbjct: 327 DIIKQLHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDN-NKRASAVLDLIHCDLRGPS 151
Query: 230 RVSSTLGYKYYVTFIDDFSRCTWITLLKDRS 260
V+S G+ Y+V F+DDFSR TW LK +S
Sbjct: 150 PVASIDGFSYFVIFVDDFSRFTWFYPLKRKS 58
>AU088980
Length = 360
Score = 87.8 bits (216), Expect = 8e-18
Identities = 42/106 (39%), Positives = 63/106 (58%)
Frame = +2
Query: 319 NGVAERKHRHLVDTTRTLLINAHAPFRFWGDAILIACYLITRMPSSVLGNEIPYSLLFPK 378
N + ERKHRH+++ TR L+ +++ P W A+ A YLI +PS +L + PY L
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 379 DPLYVVPLRVFGSTCFAHDLSPDRDKLSARAVKCVFLGYSKTQKGY 424
P ++ L+VFG+ C++ L+ + KL RA KCVFLG+ GY
Sbjct: 182 SPT-LLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>BP033602
Length = 533
Score = 79.7 bits (195), Expect = 2e-15
Identities = 49/125 (39%), Positives = 70/125 (55%), Gaps = 13/125 (10%)
Frame = +3
Query: 135 KTIGT*FES*GLYYL-----HSHPSTICGVSASPD-----IIHCRLGHPSLDKLKVLVPH 184
K IG+ GLYY + +CG+S + + H RLGHP+ L+ L P
Sbjct: 156 KMIGSAKMKDGLYYFEDTFRNKAAQGLCGMSCTSVRDQIMLWHNRLGHPNFQYLRHLFPD 335
Query: 185 LS---HLKSLDCESCQLGKHVRVSFPSRANKRSMSPFNIIHSDVWGPSRVSSTLGYKYYV 241
L + SL+CESC L K+ R + S+ S PF +IHSDVWGPS++++ G +++V
Sbjct: 336 LFKNVNCSSLECESCVLAKNQRAPYYSQPYHASR-PFYLIHSDVWGPSKITTQFGKRWFV 512
Query: 242 TFIDD 246
TFIDD
Sbjct: 513 TFIDD 527
>AV766088
Length = 501
Score = 75.1 bits (183), Expect = 6e-14
Identities = 43/102 (42%), Positives = 62/102 (60%), Gaps = 5/102 (4%)
Frame = -3
Query: 167 HCRLGHPSLDKLKVL-----VPHLSHLKSLDCESCQLGKHVRVSFPSRANKRSMSPFNII 221
H RLGHPS L+ L + + S CESC GKHVR+ F S +N ++ PF+I+
Sbjct: 301 HSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVS-SNNVTVMPFDIL 125
Query: 222 HSDVWGPSRVSSTLGYKYYVTFIDDFSRCTWITLLKDRS*LF 263
HSD+W S V S+ G+++YV F+DDF+ W L ++S +F
Sbjct: 124 HSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragment),
partial (15%)
Length = 460
Score = 70.9 bits (172), Expect = 1e-12
Identities = 54/144 (37%), Positives = 72/144 (49%), Gaps = 11/144 (7%)
Frame = +2
Query: 128 IQDRSTGKTIGT*FES*GLYYLH---SHPSTICGVSA-SPDIIHCRLGHPSLDKLKVLVP 183
IQD ST + IG ES GLY L S ST S+ SP +HCR GHPSL L+ L P
Sbjct: 32 IQDLSTPRIIGRGRESDGLYLLDEGVSKASTAYRTSSVSPFQLHCRFGHPSLGVLRKLCP 211
Query: 184 HLSHLKSLDCESCQLG-KHVRVSFPSRANKRSMSPFNIIHSDVWGPS------RVSSTLG 236
L + + CESC + R + R NKR+MS F+I+H G R+ +L
Sbjct: 212 PLESVSNFSCESCHVC*TSXRSVYLPRLNKRAMSLFHIVHL*CMGSMPCNF*IRLRVSLS 391
Query: 237 YKYYVTFIDDFSRCTWITLLKDRS 260
+ + + R W+ L+K RS
Sbjct: 392 HLWMIIL-----RAPWLYLMKSRS 448
>BP054557
Length = 538
Score = 54.7 bits (130), Expect = 8e-08
Identities = 39/127 (30%), Positives = 58/127 (44%), Gaps = 20/127 (15%)
Frame = -2
Query: 92 LNYVLFISGSPFNLISISKLTQSLNCSITFSSDSFLIQDRSTGKTIGT*FES*GLYYL-- 149
L VL++ FNLIS+S L +SLNC + + L+Q+R T KTIG E GLY +
Sbjct: 384 LAXVLYVPSFNFNLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVID 205
Query: 150 ----HSHPSTICGVSASP----------DIIHCRLGHPSLDKLKVLVPHLSHLK----SL 191
HP + ++ H RLGH S KL +L + + +
Sbjct: 204 PAVKAKHPPAEGNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKLAILQKNFPFITCKQITS 25
Query: 192 DCESCQL 198
C++C +
Sbjct: 24 PCDTCHM 4
>AV769206
Length = 536
Score = 53.1 bits (126), Expect = 2e-07
Identities = 36/86 (41%), Positives = 44/86 (50%), Gaps = 1/86 (1%)
Frame = -3
Query: 404 KLSARAVKCVFLGYSKTQKGYRCYSPSAHRFYVSKDVTFFEDRPFFASPTT-SGSTTSTT 462
KLS + +CVF+GYS KGY+C S R Y+SKDV F E R F P+ TTS+T
Sbjct: 510 KLSLHSKECVFIGYSINHKGYKCLDQSG-RIYISKDVLFHEHR--FPYPSLFPDETTSST 340
Query: 463 STTDVTTSHVMPIPLFEPFVSTQNPP 488
S S + P VS PP
Sbjct: 339 SAEFFPLSTI-------PIVSHTTPP 283
>AV427921
Length = 284
Score = 52.4 bits (124), Expect = 4e-07
Identities = 31/96 (32%), Positives = 55/96 (57%)
Frame = +1
Query: 309 QSSCPHTPQQNGVAERKHRHLVDTTRTLLINAHAPFRFWGDAILIACYLITRMPSSVLGN 368
Q + +TPQQNGV+ERK+R +++ R+LL + P F +A++ + +++ R P+ V+ N
Sbjct: 4 Q*TAAYTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQN 183
Query: 369 EIPYSLLFPKDPLYVVPLRVFGSTCFAHDLSPDRDK 404
+P + P V R+ S C A+ PD+ +
Sbjct: 184 MMPEEAWSGRQPA-VDHFRI--SRCLAYAHVPDQKR 282
>AV764934
Length = 406
Score = 48.1 bits (113), Expect = 7e-06
Identities = 32/87 (36%), Positives = 49/87 (55%), Gaps = 5/87 (5%)
Frame = -2
Query: 152 HPSTICGVSASPDIIHCRLGHPSLDKLKVLVP--HLSHLKSLDCE---SCQLGKHVRVSF 206
HPS++ + H RLGHP LD LK ++ ++S+ E +C LGK R+
Sbjct: 252 HPSSVSAF-VTLSTWHSRLGHPHLDNLKRVLSSCNVSYSPKDTVEFRTACCLGKAHRL-- 82
Query: 207 PSRANKRSMSPFNIIHSDVWGPSRVSS 233
PS+ + + PF +I+SD+WGP+ V S
Sbjct: 81 PSQMSTTTYLPFELIYSDLWGPAPVCS 1
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 43.1 bits (100), Expect = 2e-04
Identities = 18/34 (52%), Positives = 26/34 (75%)
Frame = +3
Query: 193 CESCQLGKHVRVSFPSRANKRSMSPFNIIHSDVW 226
C+SC LGKHVR+ F S + ++ PF+I+HSD+W
Sbjct: 1029 CDSCVLGKHVRLPF-SSSETITLRPFDILHSDLW 1127
>BP054351
Length = 431
Score = 38.9 bits (89), Expect = 0.004
Identities = 28/80 (35%), Positives = 39/80 (48%), Gaps = 13/80 (16%)
Frame = -1
Query: 130 DRSTGKTIGT*FES*GLYYL-----HSHPSTICGVSASPD-----IIHCRLGHPSLDKLK 179
DR +GK I + GLYY + +CG+S + + H RLGHP+ L+
Sbjct: 245 DRISGKMIASAKMKDGLYYFEDTFRNKAAQGLCGMSCTSVRDQIMLWHNRLGHPNFQYLR 66
Query: 180 VLVPHL---SHLKSLDCESC 196
L P L + SL+CESC
Sbjct: 65 HLFPDLFKNVNCSSLECESC 6
>BP053302
Length = 432
Score = 38.5 bits (88), Expect = 0.006
Identities = 31/80 (38%), Positives = 37/80 (45%), Gaps = 16/80 (20%)
Frame = -1
Query: 128 IQDRSTGKTIGT*FES*GLYYLHSH------PSTICGVSASPDII----------HCRLG 171
I D ++GK IG ES GLYYL P TI S ++ H RLG
Sbjct: 249 ILDLNSGKMIGRAKESGGLYYLDLGSASPLPPKTISSCFESFFVLNNNDDHVMLWHLRLG 70
Query: 172 HPSLDKLKVLVPHLSHLKSL 191
HPS LK L P L + K+L
Sbjct: 69 HPSFHYLKPLFPKLFYNKNL 10
>BP041939
Length = 392
Score = 38.5 bits (88), Expect = 0.006
Identities = 29/107 (27%), Positives = 49/107 (45%), Gaps = 1/107 (0%)
Frame = +3
Query: 403 DKLSARAVKCVFLGYSKTQKGYRCYSPSAHRFYVSKDVTFFEDRPFFASPTTSGSTTSTT 462
D++ ++ ++ G + C+SP R+ VS DVTFFED F +S T S
Sbjct: 36 DQVGSKVIEVCVPGLLSYLERVPCFSPDLGRYLVSADVTFFEDCSFSGWSPSSFPTYSRR 215
Query: 463 STTDVTTSHVMPIPLFEPFVSTQNPPQSQGNPEFRQYGITYEL-RHV 508
+ S +P P VS+ +P + P + G T+ + +H+
Sbjct: 216 PKSSAPPSDTQSLPSL-PLVSS-DPHDNLDLPIALRKGKTFHVFKHI 350
>BP061842
Length = 318
Score = 38.1 bits (87), Expect = 0.008
Identities = 13/29 (44%), Positives = 21/29 (71%)
Frame = -3
Query: 217 PFNIIHSDVWGPSRVSSTLGYKYYVTFID 245
P ++ D+WGP+ ST G+KYY++F+D
Sbjct: 91 PLELVPCDLWGPAPCVSTNGFKYYLSFVD 5
>BP063902
Length = 494
Score = 38.1 bits (87), Expect = 0.008
Identities = 28/75 (37%), Positives = 37/75 (49%), Gaps = 9/75 (12%)
Frame = -3
Query: 145 GLYYLHSHPSTICGVSASPDIIHCRLGHPSLDKLKVLVPHLSHLKSLDCE---------S 195
G Y + S G+++S + H RLGHP+ L HL + K + CE S
Sbjct: 237 GDLYPVTRSSPFAGLASS--VWHHRLGHPASSALN----HLRNNKLIFCEPSRSSSVCDS 76
Query: 196 CQLGKHVRVSFPSRA 210
C LGKHVR+ F S A
Sbjct: 75 CVLGKHVRLPFSSSA 31
>TC17551 weakly similar to UP|AAR24662 (AAR24662) At5g26960, partial (22%)
Length = 505
Score = 34.7 bits (78), Expect = 0.085
Identities = 27/84 (32%), Positives = 33/84 (39%), Gaps = 2/84 (2%)
Frame = +3
Query: 452 PTTSGSTTSTTSTTDVTTSHVMPIPLFEPFVSTQNPPQS--QGNPEFRQYGITYELRHVE 509
PT+S TS TS T+S P P P ++ PP S G P
Sbjct: 222 PTSSTPPTSPTSAAAATSSATPPSPSPPPTSASPPPPSSTPNGTPP------------SS 365
Query: 510 APETAPIDSNDSTPKTPATNSSDS 533
+P T P S STP +P S S
Sbjct: 366 SPATTPSPSRPSTPSSPTRASLPS 437
>AV766665
Length = 601
Score = 34.7 bits (78), Expect = 0.085
Identities = 23/56 (41%), Positives = 30/56 (53%), Gaps = 6/56 (10%)
Frame = +1
Query: 951 GIQMPIGQEMQVIDGLLLVIAFLWA------EI*FHGRAKSRLLSPDRVQKPSIEL 1000
G + +G MQ I GLLL+ W EI* HG S++ PDRV+K + EL
Sbjct: 430 GNHLWMGLHMQTIWGLLLIGYPPWGTICF*VEI**HGEVSSKI*LPDRVEKQNFEL 597
>TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II , partial
(6%)
Length = 583
Score = 34.3 bits (77), Expect = 0.11
Identities = 26/89 (29%), Positives = 39/89 (43%), Gaps = 2/89 (2%)
Frame = -1
Query: 450 ASPTTSGSTTSTTSTTDVTTSHVMPIPLFEPFVSTQ--NPPQSQGNPEFRQYGITYELRH 507
+SP+ S +T S+ S + T P P E S+ +PP S G+ +
Sbjct: 244 SSPSPSTTTASSASPSTKTLPSTRPRPASESPTSSSPSSPPPSSGS--------SVSPPR 89
Query: 508 VEAPETAPIDSNDSTPKTPATNSSDSGIV 536
+ P P +S +TP + ATN S S V
Sbjct: 88 ISPPPPPPSNSPTATPPSSATNPSSSSSV 2
>BP081717
Length = 291
Score = 34.3 bits (77), Expect = 0.11
Identities = 15/47 (31%), Positives = 26/47 (54%), Gaps = 3/47 (6%)
Frame = -1
Query: 164 DIIHCRLGHPS---LDKLKVLVPHLSHLKSLDCESCQLGKHVRVSFP 207
D+ H RLGHPS L + + P++ ++ C+ C + K ++ FP
Sbjct: 171 DMWHFRLGHPSSKVLQHISKVFPYVKLHSNIICDFCHMAKQTKLPFP 31
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.342 0.151 0.501
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,901,429
Number of Sequences: 28460
Number of extensions: 355715
Number of successful extensions: 3299
Number of sequences better than 10.0: 72
Number of HSP's better than 10.0 without gapping: 3189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3271
length of query: 1105
length of database: 4,897,600
effective HSP length: 100
effective length of query: 1005
effective length of database: 2,051,600
effective search space: 2061858000
effective search space used: 2061858000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC147007.6