BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147002.5 + phase: 0
(143 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14173 homologue to UP|RL10_SOLME (P93847) 60S ribosomal protei... 224 6e-60
TC14172 homologue to UP|RL10_VITRI (Q9SPB3) 60S ribosomal protei... 218 4e-58
TC14171 homologue to UP|RL10_EUPES (Q9M5M7) 60S ribosomal protei... 199 2e-52
TC12431 similar to UP|Q86QS5 (Q86QS5) Ribosomal protein L10, par... 151 4e-38
TC10612 homologue to UP|RL10_VITRI (Q9SPB3) 60S ribosomal protei... 142 3e-35
TC8687 similar to UP|Q9SXL2 (Q9SXL2) Serine decarboxylase, parti... 26 2.3
BP064703 26 2.3
TC8963 homologue to UP|TBB3_SOYBN (P28551) Tubulin beta chain (F... 26 3.0
AV770154 25 3.9
TC17492 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal protei... 25 3.9
AU089231 25 6.7
BP075651 25 6.7
TC14476 homologue to UP|TBB3_WHEAT (Q9ZRB0) Tubulin beta-3 chain... 24 8.7
BP065600 24 8.7
BP054252 24 8.7
TC11916 similar to UP|P94078 (P94078) Alpha-mannosidase (AT3G267... 24 8.7
>TC14173 homologue to UP|RL10_SOLME (P93847) 60S ribosomal protein L10
(EQM), partial (90%)
Length = 611
Score = 224 bits (570), Expect = 6e-60
Identities = 108/122 (88%), Positives = 112/122 (91%)
Frame = +2
Query: 1 MSKFAGKDTFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPLGTCARVSIGQVL 60
M+KFAGKD FHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKP GTCARV+IGQVL
Sbjct: 245 MAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVL 424
Query: 61 LSVRCKSGNGDHAQEALRRAKFKFPGRQKIIVSRKWGFTKLSHAEYLKLKSENRIVPDGV 120
LSVRCK N HAQEALRRAKFKFPGRQKIIVSRKWGFTK S +YLK KSENRI+PDGV
Sbjct: 425 LSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRKWGFTKFSRTDYLKYKSENRILPDGV 604
Query: 121 NA 122
NA
Sbjct: 605 NA 610
>TC14172 homologue to UP|RL10_VITRI (Q9SPB3) 60S ribosomal protein L10 (QM
protein homolog), partial (54%)
Length = 751
Score = 218 bits (554), Expect = 4e-58
Identities = 104/119 (87%), Positives = 110/119 (92%)
Frame = +1
Query: 25 NKMLSCAGADRLQTGMRGAFGKPLGTCARVSIGQVLLSVRCKSGNGDHAQEALRRAKFKF 84
NKMLSCAGADRLQTGMRGAFGKP GTCARV+IGQVLLSVRCK N HAQEALRRAKFKF
Sbjct: 1 NKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKF 180
Query: 85 PGRQKIIVSRKWGFTKLSHAEYLKLKSENRIVPDGVNAKVLGCHGPLANRQPGRAFLPA 143
PGRQKIIVSRKWGFTK S A+YL+ KSENRI+PDGVNAK+LGCHGPLANRQPGRAF+ A
Sbjct: 181 PGRQKIIVSRKWGFTKFSRADYLRYKSENRILPDGVNAKLLGCHGPLANRQPGRAFIDA 357
>TC14171 homologue to UP|RL10_EUPES (Q9M5M7) 60S ribosomal protein L10,
partial (78%)
Length = 716
Score = 199 bits (505), Expect = 2e-52
Identities = 103/134 (76%), Positives = 110/134 (81%), Gaps = 3/134 (2%)
Frame = +1
Query: 1 MSKFAGKDTFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPLGTCARVSIGQVL 60
M+KFAGKD FHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKP GTCARV+IGQVL
Sbjct: 277 MAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVL 456
Query: 61 LSVRCKSGNGDHAQEALRRAKFKFPGRQKIIVSRKWG-FTKLSHAEYLKLKSENRIVPDG 119
LSVRCK N HAQEALRRAKFKFPGRQKIIVSRKWG + A+YL +S N I+P
Sbjct: 457 LSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRKWGIYPXFLXADYLXYQSXNXILPRM 636
Query: 120 V-NAKVL-GCHGPL 131
V NA GCHG +
Sbjct: 637 VLNAXXFXGCHGTI 678
>TC12431 similar to UP|Q86QS5 (Q86QS5) Ribosomal protein L10, partial (46%)
Length = 380
Score = 151 bits (382), Expect = 4e-38
Identities = 75/111 (67%), Positives = 83/111 (74%)
Frame = +1
Query: 21 VLRINKMLSCAGADRLQTGMRGAFGKPLGTCARVSIGQVLLSVRCKSGNGDHAQEALRRA 80
VLRI KMLSCAGADRLQTGMRGAFGKP GT ARV IGQ LLSVR K N EALRRA
Sbjct: 1 VLRITKMLSCAGADRLQTGMRGAFGKPQGTVARVKIGQTLLSVRSKDANKPFVLEALRRA 180
Query: 81 KFKFPGRQKIIVSRKWGFTKLSHAEYLKLKSENRIVPDGVNAKVLGCHGPL 131
K+KFPGRQK+ VS WGFT+ + EY+ LKS +I+PDG KV+ GPL
Sbjct: 181 KYKFPGRQKLFVSANWGFTRFTREEYVALKSAGKIIPDGAYCKVVSTKGPL 333
>TC10612 homologue to UP|RL10_VITRI (Q9SPB3) 60S ribosomal protein L10 (QM
protein homolog), partial (68%)
Length = 474
Score = 142 bits (357), Expect = 3e-35
Identities = 68/74 (91%), Positives = 69/74 (92%)
Frame = +2
Query: 1 MSKFAGKDTFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPLGTCARVSIGQVL 60
M+KFAGKD FHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKP GTCARVSIGQVL
Sbjct: 251 MAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVSIGQVL 430
Query: 61 LSVRCKSGNGDHAQ 74
LSVRCK GN HAQ
Sbjct: 431 LSVRCKDGNAVHAQ 472
>TC8687 similar to UP|Q9SXL2 (Q9SXL2) Serine decarboxylase, partial (80%)
Length = 1644
Score = 26.2 bits (56), Expect = 2.3
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 77 LRRAKFKFPGRQKIIVSRKWGFTKLSHAEYLKLK 110
++ FK PG I+ KW +K+ + + LKL+
Sbjct: 534 IQNTNFKLPGMDTIVAFNKW-ISKVVYGKVLKLR 436
>BP064703
Length = 490
Score = 26.2 bits (56), Expect = 2.3
Identities = 15/60 (25%), Positives = 23/60 (38%)
Frame = +2
Query: 82 FKFPGRQKIIVSRKWGFTKLSHAEYLKLKSENRIVPDGVNAKVLGCHGPLANRQPGRAFL 141
F FP Q+ S+KW + +++L PD + GC P AF+
Sbjct: 65 FPFPSSQRFTKSKKWWWIHEDESQHLP--------PDSALSNADGCGAPKQGEFSREAFI 220
>TC8963 homologue to UP|TBB3_SOYBN (P28551) Tubulin beta chain (Fragment),
partial (75%)
Length = 985
Score = 25.8 bits (55), Expect = 3.0
Identities = 21/75 (28%), Positives = 32/75 (42%)
Frame = -1
Query: 66 KSGNGDHAQEALRRAKFKFPGRQKIIVSRKWGFTKLSHAEYLKLKSENRIVPDGVNAKVL 125
+ GNG + RR++F +PG+ R+ T L A+ L+S N V K +
Sbjct: 859 RRGNGMRLTASFRRSEFSWPGK------RRQQVTPLMVADIR*LRSPNVGVASFKVLKQM 698
Query: 126 GCHGPLANRQPGRAF 140
L+N AF
Sbjct: 697 S*RASLSNTMHSSAF 653
>AV770154
Length = 378
Score = 25.4 bits (54), Expect = 3.9
Identities = 10/37 (27%), Positives = 18/37 (48%)
Frame = -1
Query: 28 LSCAGADRLQTGMRGAFGKPLGTCARVSIGQVLLSVR 64
LSC ++ +FG P G+C ++ +G + R
Sbjct: 327 LSCGSGQKIVAVEFASFGNPPGSCGKLVLGDCNAAAR 217
>TC17492 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal protein S25-1,
complete
Length = 559
Score = 25.4 bits (54), Expect = 3.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 66 KSGNGDHAQEALRRAKFKFPGRQKIIV 92
KSG+G H +E + + K K G+Q +V
Sbjct: 95 KSGDGKHEEEEVEQGKAKGEGQQHGVV 175
>AU089231
Length = 383
Score = 24.6 bits (52), Expect = 6.7
Identities = 9/30 (30%), Positives = 16/30 (53%)
Frame = +1
Query: 28 LSCAGADRLQTGMRGAFGKPLGTCARVSIG 57
LSC + + +FG P G+C ++ +G
Sbjct: 214 LSCGSGNXIVAVEFASFGNPSGSCGKLVLG 303
>BP075651
Length = 475
Score = 24.6 bits (52), Expect = 6.7
Identities = 9/30 (30%), Positives = 16/30 (53%)
Frame = -3
Query: 28 LSCAGADRLQTGMRGAFGKPLGTCARVSIG 57
LSC ++ +FG P G+C ++ +G
Sbjct: 386 LSCGSGKKIVAVEFASFGNPPGSCGKLVLG 297
>TC14476 homologue to UP|TBB3_WHEAT (Q9ZRB0) Tubulin beta-3 chain (Beta-3
tubulin), complete
Length = 2208
Score = 24.3 bits (51), Expect = 8.7
Identities = 15/49 (30%), Positives = 23/49 (46%), Gaps = 12/49 (24%)
Frame = -3
Query: 66 KSGNGDHAQEALRRAKFKFPGRQK------IIVSRKW------GFTKLS 102
+ G G +LRR++ +PG ++ I+V KW GF LS
Sbjct: 853 RRGKGIRLTASLRRSELSWPGNRRQHVTPLIVVEIKWFRSPKLGFVSLS 707
>BP065600
Length = 486
Score = 24.3 bits (51), Expect = 8.7
Identities = 8/22 (36%), Positives = 15/22 (67%)
Frame = -1
Query: 107 LKLKSENRIVPDGVNAKVLGCH 128
++++ + R V G+N +LGCH
Sbjct: 333 IQIEDKPRSVQGGMNQTILGCH 268
>BP054252
Length = 559
Score = 24.3 bits (51), Expect = 8.7
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -1
Query: 10 FHLRVRVHPFHVLRINKMLSC 30
FH+R R H F RI + LSC
Sbjct: 106 FHVRFREHFFFSSRILRSLSC 44
>TC11916 similar to UP|P94078 (P94078) Alpha-mannosidase
(AT3G26720/MLJ15_12), partial (14%)
Length = 547
Score = 24.3 bits (51), Expect = 8.7
Identities = 8/25 (32%), Positives = 17/25 (68%)
Frame = +1
Query: 101 LSHAEYLKLKSENRIVPDGVNAKVL 125
++ +EY++ + RIVPD +N ++
Sbjct: 100 VAESEYIEYNTTQRIVPDKINVHLV 174
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.140 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,507,039
Number of Sequences: 28460
Number of extensions: 30655
Number of successful extensions: 170
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of query: 143
length of database: 4,897,600
effective HSP length: 82
effective length of query: 61
effective length of database: 2,563,880
effective search space: 156396680
effective search space used: 156396680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)
Medicago: description of AC147002.5