BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147000.7 - phase: 0
(1185 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 120 2e-27
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 100 1e-21
AV780009 90 2e-18
BP075943 89 3e-18
BP063694 71 9e-13
AV427921 64 1e-10
BP084332 63 2e-10
AV424544 60 2e-09
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 53 3e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 52 4e-07
BP033602 49 5e-06
AV766088 49 6e-06
BP052632 48 8e-06
BP043850 47 2e-05
AV769206 45 9e-05
TC9039 43 3e-04
TC18909 40 8e-04
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 41 0.001
AV427422 38 0.011
BF177840 37 0.018
>BP066094
Length = 532
Score = 120 bits (300), Expect = 2e-27
Identities = 56/149 (37%), Positives = 94/149 (62%)
Frame = +2
Query: 760 TRITSIRVLISLAAIHNLIVHQMDVKTAFLNGELEEEIYMDQPEGFVIHGQENKVCKLDK 819
++ +IR+LIS + HN+I+HQM+VK+AFLNG + EE+Y+ QP G + + KL K
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 820 SLYGLKQAPKQWHEKFDNLMIENEFKVNESDKCIYSKYENNTCTIICLYVDDLLIFGSNL 879
SLYGLKQAP+ W+E+ + ++ENE + D ++ K + I+ +YVDD++ +N
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 880 NAIKDVKSLLCHNFDMKDLGKADVILGIK 908
+ K+ ++ F+M+ +G+ LGI+
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 100 bits (249), Expect = 1e-21
Identities = 59/154 (38%), Positives = 91/154 (58%), Gaps = 13/154 (8%)
Frame = +2
Query: 835 FDNLMIENEFKVNESDKCIYSK-YENNTCTIICLYVDDLLIFGSNLNAIKDVKSLLCHNF 893
FD+ ++ + SD C Y K +++N I+ LYVDD+L+ G N + ++++K+ L F
Sbjct: 2 FDSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREF 181
Query: 894 DMKDLGKADVILGIKI--TRTDNGISLNQSHYVEKILRKYNYFYCKPASTPCDPSVKLFK 951
DMKDLG A+ ILG++I R D I L+Q +Y++K+LR++N C P STP + KL
Sbjct: 182 DMKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSS 361
Query: 952 NTGDS-------VRQTEYASIIGSLRYA---TDC 975
+ S + + YAS +GSL YA T+C
Sbjct: 362 SMIPSSEAERMEMSRVPYASAVGSLMYAM*RTNC 463
>AV780009
Length = 529
Score = 89.7 bits (221), Expect = 2e-18
Identities = 55/165 (33%), Positives = 86/165 (51%), Gaps = 1/165 (0%)
Frame = -1
Query: 1000 QAIERVMRYLKKTMTLGLHYQR-YPAVLEGYSDADWNNLSDDSKATSGYIFSIAGGAVSW 1058
QA RV+RY+K GL + P L+ YSD+DW D ++ +GY + +SW
Sbjct: 529 QAATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISW 350
Query: 1059 KSKKQTILAQSTMESEMIALAAASEEASWLRCLLSEIPLWERPLPAVLIHCDSTAAIAKI 1118
++KKQT +++S+ E+E ALAA E WL L + L PLP V + CD+ +A+
Sbjct: 349 RTKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKL-NVPLP-VPLFCDNQSALHIA 176
Query: 1119 ENRYYNGKRRQIRRKHSTIREYLSNGTVRVDFVRTNENLADPLTK 1163
N ++ + + I +R L G + + + T+ LAD TK
Sbjct: 175 HNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTK 41
>BP075943
Length = 547
Score = 89.4 bits (220), Expect = 3e-18
Identities = 59/159 (37%), Positives = 90/159 (56%), Gaps = 2/159 (1%)
Frame = -3
Query: 863 TIICLYVDDLLIFGSNLNAIKDVKSLLCHNFDMKDLGKADVILGIKITRTDNGISLNQSH 922
T + LYVDD+L+ G++++ I+ VKS L F +KDLG+A LG++I R+ +GI LNQ
Sbjct: 527 TALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRK 348
Query: 923 YVEKILRKYNYFYCKPA-STPCDPSVKLFKNTGDSVRQT-EYASIIGSLRYATDCTRPDI 980
Y +++ +F +P + S L NTG + Y I+G L Y + TRPDI
Sbjct: 347 YALQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLY-LNTTRPDI 171
Query: 981 SYAVGLLCKFTSRPSMEHWQAIERVMRYLKKTMTLGLHY 1019
++AV L +F S P+ H Q + ++YL + GL Y
Sbjct: 170 TFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFY 54
>BP063694
Length = 511
Score = 71.2 bits (173), Expect = 9e-13
Identities = 46/160 (28%), Positives = 78/160 (48%), Gaps = 6/160 (3%)
Frame = -2
Query: 289 KNGIFVGKGYATDGMFKLNIDMNKI--SSSAYMLCDFNIWHSRLCHVNKRIISNMSGLGL 346
K G +G+ G++ LN D + +SS F +WHSRL HVN II + G
Sbjct: 474 KTGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNFDIIKQLHKHGC 295
Query: 347 IPKISLNDFEKCQFCSQAKINKES----HKSVTRITEPFELIHSDLCELDGNLTRNGKRY 402
+ S+ C C+ ++ K H + R + +LIH DL + +G Y
Sbjct: 294 LDVSSILPKPIC--CTSCQMAKSKRLVFHDNNKRASAVLDLIHCDLRGPSPVASIDGFSY 121
Query: 403 FITFIDDCSDYTHVYLMRNKNEALDIFKQYVKEIENQFNI 442
F+ F+DD S +T Y ++ K++ D+ ++ +EN+F++
Sbjct: 120 FVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMENRFSV 1
>AV427921
Length = 284
Score = 64.3 bits (155), Expect = 1e-10
Identities = 35/91 (38%), Positives = 49/91 (53%), Gaps = 1/91 (1%)
Frame = +1
Query: 474 TAPYSPEMNGKAERKNRTFTELVVATMLNSGAAPHWWGEILLTVCYVLNRVPK-TKNKIS 532
TA Y+P+ NG +ERKNRT +V + + SG + E ++ ++LNR P +
Sbjct: 10 TAAYTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMM 189
Query: 533 PYEILKKRQPNLSYFRTWGCLAYVRKPDPKR 563
P E RQP + +FR CLAY PD KR
Sbjct: 190 PEEAWSGRQPAVDHFRISRCLAYAHVPDQKR 282
>BP084332
Length = 368
Score = 63.2 bits (152), Expect = 2e-10
Identities = 35/107 (32%), Positives = 60/107 (55%)
Frame = +2
Query: 1063 QTILAQSTMESEMIALAAASEEASWLRCLLSEIPLWERPLPAVLIHCDSTAAIAKIENRY 1122
Q+ +A ST E+E I+ A S + W++ L + + E +P I+CD+TAAI+ +N
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIP---IYCDNTAAISLSKNPI 202
Query: 1123 YNGKRRQIRRKHSTIREYLSNGTVRVDFVRTNENLADPLTKGLNREK 1169
+ + + I K+ IR+Y+ G + + FV T+ AD TK L ++
Sbjct: 203 LHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDR 343
>AV424544
Length = 276
Score = 60.1 bits (144), Expect = 2e-09
Identities = 28/74 (37%), Positives = 49/74 (65%)
Frame = +3
Query: 850 DKCIYSKYENNTCTIICLYVDDLLIFGSNLNAIKDVKSLLCHNFDMKDLGKADVILGIKI 909
D +++K+ + + T+I +YVDDL++ G++LN I+ VK+ L F +KDLG LG+++
Sbjct: 54 DHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQFRIKDLGTLKYFLGLEV 233
Query: 910 TRTDNGISLNQSHY 923
R+ G+ L+Q Y
Sbjct: 234 ARSSCGLFLSQRKY 275
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 52.8 bits (125), Expect = 3e-07
Identities = 33/123 (26%), Positives = 62/123 (49%)
Frame = +3
Query: 1048 IFSIAGGAVSWKSKKQTILAQSTMESEMIALAAASEEASWLRCLLSEIPLWERPLPAVLI 1107
+ + AGGAV+W S+ Q +A ST E+E IA A E W++ L P+ ++
Sbjct: 3 LVTFAGGAVAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSHPILCCIV 182
Query: 1108 HCDSTAAIAKIENRYYNGKRRQIRRKHSTIREYLSNGTVRVDFVRTNENLADPLTKGLNR 1167
+ +A+I + + +R+ L++ + ++ + T+++ AD +TK L R
Sbjct: 183 ITKALFTLARI--LLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPR 356
Query: 1168 EKV 1170
EK+
Sbjct: 357 EKL 365
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 52.4 bits (124), Expect = 4e-07
Identities = 47/143 (32%), Positives = 65/143 (44%), Gaps = 2/143 (1%)
Frame = -1
Query: 920 QSHYVEKILRKYNYFYCKPASTPCDPSVKLF--KNTGDSVRQTEYASIIGSLRYATDCTR 977
Q YV+ IL+K+ K ST +N G V T Y S+IGS+RY + R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRY-LNTVR 563
Query: 978 PDISYAVGLLCKFTSRPSMEHWQAIERVMRYLKKTMTLGLHYQRYPAVLEGYSDADWNNL 1037
DI VGL +F P H Q +R +RY+K T+ G+ + V+ N+L
Sbjct: 562 SDIVCGVGLRSRF-MEP*DCH*QGAQRSLRYIKGTLKDGI----FMLVI-----TM*NSL 413
Query: 1038 SDDSKATSGYIFSIAGGAVSWKS 1060
GY F + GA+SW S
Sbjct: 412 DTQIVTRPGYAFHLGRGAISWSS 344
>BP033602
Length = 533
Score = 48.9 bits (115), Expect = 5e-06
Identities = 30/86 (34%), Positives = 47/86 (53%), Gaps = 1/86 (1%)
Frame = +3
Query: 325 IWHSRLCHVNKRIISNMSGLGLIPKISLNDFEKCQFCSQAKINKESHKSVT-RITEPFEL 383
+WH+RL H N + + ++ L ++ + E C+ C AK + + S + PF L
Sbjct: 276 LWHNRLGHPNFQYLRHLFP-DLFKNVNCSSLE-CESCVLAKNQRAPYYSQPYHASRPFYL 449
Query: 384 IHSDLCELDGNLTRNGKRYFITFIDD 409
IHSD+ T+ GKR+F+TFIDD
Sbjct: 450 IHSDVWGPSKITTQFGKRWFVTFIDD 527
>AV766088
Length = 501
Score = 48.5 bits (114), Expect = 6e-06
Identities = 29/102 (28%), Positives = 51/102 (49%), Gaps = 1/102 (0%)
Frame = -3
Query: 324 NIWHSRLCHVNKRIISNMSGLGLIPKISLNDFEKCQFCSQAKINKESHKSVTRIT-EPFE 382
++WHSRL H + + + I LN C+ C K + S +T PF+
Sbjct: 310 SLWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFD 131
Query: 383 LIHSDLCELDGNLTRNGKRYFITFIDDCSDYTHVYLMRNKNE 424
++HSDL L+ G R+++ F+DD +D+ + + NK++
Sbjct: 130 ILHSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQ 8
>BP052632
Length = 489
Score = 48.1 bits (113), Expect = 8e-06
Identities = 22/72 (30%), Positives = 43/72 (59%)
Frame = -3
Query: 532 SPYEILKKRQPNLSYFRTWGCLAYVRKPDPKRVKLASRAYECAFIGYALNSKAYRFYDLK 591
+PYE+ + R+P +SYF +GC ++ + K ++ + +GY+ NSKAY ++ +
Sbjct: 484 TPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNSR 305
Query: 592 SKTIIESNDVDF 603
++ + ES +V F
Sbjct: 304 TQVVEESINVKF 269
>BP043850
Length = 515
Score = 47.0 bits (110), Expect = 2e-05
Identities = 31/87 (35%), Positives = 45/87 (51%), Gaps = 1/87 (1%)
Frame = -1
Query: 1084 EASWLRCLLSEIP-LWERPLPAVLIHCDSTAAIAKIENRYYNGKRRQIRRKHSTIREYLS 1142
E WLR LLSE+ L +P P +H D+T+AI N Y+ R I ++RE
Sbjct: 512 EIIWLRGLLSELGFLQSQPTP---LHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYD 342
Query: 1143 NGTVRVDFVRTNENLADPLTKGLNREK 1169
+ + V T+ +AD LTK L R++
Sbjct: 341 RRVITLPHVSTSVQIADILTKSLTRQR 261
>AV769206
Length = 536
Score = 44.7 bits (104), Expect = 9e-05
Identities = 22/48 (45%), Positives = 32/48 (65%)
Frame = -3
Query: 565 KLASRAYECAFIGYALNSKAYRFYDLKSKTIIESNDVDFYENKFPFKS 612
KL+ + EC FIGY++N K Y+ D +S I S DV F+E++FP+ S
Sbjct: 510 KLSLHSKECVFIGYSINHKGYKCLD-QSGRIYISKDVLFHEHRFPYPS 370
>TC9039
Length = 1218
Score = 42.7 bits (99), Expect = 3e-04
Identities = 24/77 (31%), Positives = 39/77 (50%)
Frame = +3
Query: 221 DQKVLLGDSHSTDVVGIGDIELKFTSEKTLILKDVLHTPKIRKNLVSGFLLNKAGFTQSI 280
++ + +GD + +V IG L + L LKD P R+NL+S L+K+G++ S
Sbjct: 951 ERYIYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLDKSGYSCSF 1130
Query: 281 GADLYTITKNGIFVGKG 297
G ++ N VG G
Sbjct: 1131 GNSKAELSFNSNIVGTG 1181
Score = 42.7 bits (99), Expect = 3e-04
Identities = 35/145 (24%), Positives = 52/145 (35%)
Frame = +2
Query: 14 LQQIAHEIIAEGMALPEQFQIAVIIDKLPPAWKDFKSLLRHKTKEFSLESLITRLRIEEE 73
+ IA ++ A + L + I +++ LP + FK +++SL LI+ EEE
Sbjct: 527 MSNIASKLKALKLELSDDLLIHLVLLSLPAQFSQFKISYNCPKEKWSLNELISFCVQEEE 706
Query: 74 ARKQEQNEEVFVVSNNNTKKKFVGAVLKPAGKPFKNQNRPMNKNSNRNKTGNNSRPQIQQ 133
KQE+ E VS + K K R KT
Sbjct: 707 RLKQERKESAHFVSTSKDKGK-------------------------RKKTVEPKNEAADA 811
Query: 134 PPKNDAAPPFNCYNCGQADHMARKC 158
P CY C + HM +KC
Sbjct: 812 PAPKKQKEDDTCYFCNVSGHMKKKC 886
>TC18909
Length = 621
Score = 40.0 bits (92), Expect = 0.002
Identities = 28/85 (32%), Positives = 41/85 (47%), Gaps = 2/85 (2%)
Frame = +2
Query: 976 TRPDISYAVGLLCKFTSRPSMEHWQAIERVMRYLKKTM-TLGLHYQ-RYPAVLEGYSDAD 1033
T P IS++V +C F S EH A + ++ K + T+G Q +PA +SDAD
Sbjct: 326 TSPQISFSVNKVC*FLSESLEEHGTAGKCILNVT*KAL*TMGFFIQTNFPA----FSDAD 493
Query: 1034 WNNLSDDSKATSGYIFSIAGGAVSW 1058
W + DD ++T I G W
Sbjct: 494 WASNVDDRRSTFWEIVFTLGPKFRW 568
Score = 33.5 bits (75), Expect(2) = 8e-04
Identities = 15/17 (88%), Positives = 16/17 (93%)
Frame = +1
Query: 730 GSIDKYKARLVAKGFRQ 746
GSI+KYKARLVAKGF Q
Sbjct: 1 GSINKYKARLVAKGFHQ 51
Score = 26.9 bits (58), Expect(2) = 8e-04
Identities = 10/24 (41%), Positives = 18/24 (74%)
Frame = +2
Query: 751 DFFDTYSPVTRITSIRVLISLAAI 774
D+ +T SPV + ++R+L+SLA +
Sbjct: 62 DYTETVSPVVKPVTVRILLSLAVM 133
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 40.8 bits (94), Expect = 0.001
Identities = 25/97 (25%), Positives = 51/97 (51%), Gaps = 1/97 (1%)
Frame = +1
Query: 1087 WLRCLLSEIPL-WERPLPAVLIHCDSTAAIAKIENRYYNGKRRQIRRKHSTIREYLSNGT 1145
WL LL ++ + +E+P L++CD+ +A N ++ + + I +RE + G
Sbjct: 4 WLTYLLQDLKVPFEQP---ALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGL 174
Query: 1146 VRVDFVRTNENLADPLTKGLNREKVANTSSRMGLMPI 1182
+ + + ++E LAD TK L+ + +++GL+ I
Sbjct: 175 IHLLPISSSEPLADIYTKALSPQNFHQICAKLGLINI 285
>AV427422
Length = 417
Score = 37.7 bits (86), Expect = 0.011
Identities = 31/126 (24%), Positives = 53/126 (41%), Gaps = 5/126 (3%)
Frame = +1
Query: 399 GKRYFITFIDDCSDYTHVYLMRNKNEALDIFKQYVKEIENQFNIRIKRFRSDRGTEYGSH 458
G + +D S ++H +++ A I +V+E+ + + SDR + S+
Sbjct: 25 GYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLS-IVSDRDPLFMSN 201
Query: 459 IFNEYYKELGIIHETTAPYSPEMNGKAERKNR---TFTELVVATMLNSGAAPHW--WGEI 513
+ E +K G + + Y PE +G+ E NR T+ +A S A HW W E
Sbjct: 202 FWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWA--HWVPWAEY 375
Query: 514 LLTVCY 519
Y
Sbjct: 376 WYNTSY 393
>BF177840
Length = 410
Score = 37.0 bits (84), Expect = 0.018
Identities = 26/96 (27%), Positives = 49/96 (50%), Gaps = 2/96 (2%)
Frame = +2
Query: 449 SDRGTEYGSHIFNEYYKELG--IIHETTAPYSPEMNGKAERKNRTFTELVVATMLNSGAA 506
SDR T++ SH + + ++G +++ TT P+ +G+ E N+T + L+ + + +
Sbjct: 23 SDRDTKFISHFWRTLWGKVGTKLLYSTTC--HPQTDGQTEVVNKTLSTLLRSVLERN--- 187
Query: 507 PHWWGEILLTVCYVLNRVPKTKNKISPYEILKKRQP 542
W L + + NRV + K SP+EI+ P
Sbjct: 188 LKMWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNP 295
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.134 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,575,644
Number of Sequences: 28460
Number of extensions: 267905
Number of successful extensions: 1442
Number of sequences better than 10.0: 70
Number of HSP's better than 10.0 without gapping: 1406
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1428
length of query: 1185
length of database: 4,897,600
effective HSP length: 100
effective length of query: 1085
effective length of database: 2,051,600
effective search space: 2225986000
effective search space used: 2225986000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC147000.7