BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147000.5 - phase: 0
(221 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin at... 32 0.066
TC10339 homologue to UP|RL24_CICAR (O65743) 60S ribosomal protei... 32 0.11
TC20273 similar to UP|REMO_SOLTU (P93788) Remorin (pp34), partia... 29 0.56
TC12805 28 0.96
CB828471 27 2.1
TC15487 similar to UP|O23144 (O23144) Proton pump interactor (AT... 27 3.6
TC15842 homologue to GB|BAB62912.1|15207792|AB055883 ERF domain ... 26 4.7
TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, p... 26 4.7
AV417976 26 6.2
AV414661 25 8.1
TC17693 similar to UP|SFR2_HUMAN (Q01130) Splicing factor, argin... 25 8.1
BP032174 25 8.1
>TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin attachment
subunit precursor, partial (3%)
Length = 1235
Score = 32.3 bits (72), Expect = 0.066
Identities = 22/81 (27%), Positives = 40/81 (49%)
Frame = +1
Query: 68 KHYQLKALKLLDDILENTIEIVKEPIPVLDEDPNVDDCGIRLFRHSKPGIVFDHADEPQP 127
K + + ++ D I E +E + LD+D +DD G+ +PG + + +EP
Sbjct: 562 KDHDGEVKEMWDTIAGGDSEDDQEGVRNLDDDNFIDDTGV------EPGF-YGNYNEPSS 720
Query: 128 PMKRPKLVPGEDIDEKSKKFR 148
P + P+ GE+ DE + F+
Sbjct: 721 PGEAPQAEEGEEDDEINDLFK 783
>TC10339 homologue to UP|RL24_CICAR (O65743) 60S ribosomal protein L24,
complete
Length = 677
Score = 31.6 bits (70), Expect = 0.11
Identities = 22/81 (27%), Positives = 40/81 (49%)
Frame = +2
Query: 138 EDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKL 197
+DI +++ K RRR + + A KK + E +DAA +A + +ERI+K
Sbjct: 260 KDIAQEAVKKRRRATKKPYSRSIVGATLEVIQKKRTEKPEVRDAAREAALREIKERIKKT 439
Query: 198 KKIRGERWLPSMAKEMQAKIK 218
K + + + AK +++ K
Sbjct: 440 KDEKKAKKAETTAKSQKSQGK 502
>TC20273 similar to UP|REMO_SOLTU (P93788) Remorin (pp34), partial (64%)
Length = 569
Score = 29.3 bits (64), Expect = 0.56
Identities = 43/170 (25%), Positives = 69/170 (40%), Gaps = 6/170 (3%)
Frame = +1
Query: 57 NDNQNPKSPKIKHYQLKALKLLDDILENTIEIVKEPIPVLDEDPNVDDCGIRLFRHSKPG 116
ND K+P++ KAL +V E PV + P+ SK
Sbjct: 130 NDAVAKKAPEVAADDSKALV-----------VVPEKTPVPENKPS-----------SKGS 243
Query: 117 IVFDHADEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARL 176
+ D A KR V + EKSK + ++++ D++A N
Sbjct: 244 LDRDVALAELEKEKRLSYVKAWEESEKSKTENKAQKNLS----DVVAWEN---------- 381
Query: 177 EAKDAAAKAKAKREEERIEKLKKIRGERW------LPSMAKEMQAKIKIK 220
+K AA +A+ ++ EER+EK K GE+ + A+E +A I+ K
Sbjct: 382 -SKKAALEAQLRKIEERLEKKKAEYGEKMKNKIALVHKEAEERRAMIEAK 528
>TC12805
Length = 702
Score = 28.5 bits (62), Expect = 0.96
Identities = 12/28 (42%), Positives = 18/28 (63%)
Frame = +1
Query: 127 PPMKRPKLVPGEDIDEKSKKFRRRIRSI 154
PP K+PK +P D + K K+ RR+R +
Sbjct: 91 PPAKQPKPLPVPDSEPKKKRGGRRLRKM 174
>CB828471
Length = 525
Score = 27.3 bits (59), Expect = 2.1
Identities = 8/22 (36%), Positives = 17/22 (76%)
Frame = +1
Query: 45 SATNNNDTKKKHNDNQNPKSPK 66
++ +N D KK+H +++ P+SP+
Sbjct: 271 ASKSNKDAKKEHQEDEGPRSPQ 336
>TC15487 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (16%)
Length = 1153
Score = 26.6 bits (57), Expect = 3.6
Identities = 25/80 (31%), Positives = 40/80 (49%), Gaps = 1/80 (1%)
Frame = +2
Query: 120 DHADEPQPPMKRPKLVPGE-DIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEA 178
+ ADE + + R + P E +ID K +R + IA A A+++ + A
Sbjct: 362 EEADEYEFEVPRKETPPKEPEIDPAKLKEMKR--------EEEIAKAKQAWERK--KKLA 511
Query: 179 KDAAAKAKAKREEERIEKLK 198
+ AAAKA K ++E +KLK
Sbjct: 512 EKAAAKAALKAQKEAEKKLK 571
>TC15842 homologue to GB|BAB62912.1|15207792|AB055883 ERF domain protein12
{Arabidopsis thaliana;} , partial (41%)
Length = 746
Score = 26.2 bits (56), Expect = 4.7
Identities = 19/62 (30%), Positives = 30/62 (47%)
Frame = -2
Query: 121 HADEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKD 180
H +PQ R V G+ ++SKK RRR R + D+ A +D + L R++ +
Sbjct: 655 HGSKPQ--WWRLVQVDGDSTPDESKKRRRRGRFCNLSDGDVRGADSDGDWRRLRRVDVFE 482
Query: 181 AA 182
A
Sbjct: 481 DA 476
>TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, partial (8%)
Length = 769
Score = 26.2 bits (56), Expect = 4.7
Identities = 13/45 (28%), Positives = 21/45 (45%), Gaps = 1/45 (2%)
Frame = +2
Query: 85 TIEIVKEPIPV-LDEDPNVDDCGIRLFRHSKPGIVFDHADEPQPP 128
+ E+ +E + V +D+ P L S P + DH +P PP
Sbjct: 158 SFEVEEELVEVRVDKQPETQVASPTLSEPSSPSPLHDHVHQPSPP 292
>AV417976
Length = 347
Score = 25.8 bits (55), Expect = 6.2
Identities = 12/45 (26%), Positives = 19/45 (41%)
Frame = +1
Query: 113 SKPGIVFDHADEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVD 157
+KP ++ DH+ P P K +P K + F + S D
Sbjct: 130 TKPPLMADHSSNPSPNQNCAKTIPSLFGSPKFRDFTNKCLSAGTD 264
>AV414661
Length = 387
Score = 25.4 bits (54), Expect = 8.1
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -3
Query: 38 TSFMNGFSATNNNDTKKKHNDNQNPKS 64
+ F + + N T +K+ NQNPKS
Sbjct: 85 SQFHENLTKSTRNKTPQKNRKNQNPKS 5
>TC17693 similar to UP|SFR2_HUMAN (Q01130) Splicing factor,
arginine/serine-rich 2 (Splicing factor SC35) (SC-35)
(Splicing component, 35 kDa) (PR264 protein), partial
(8%)
Length = 751
Score = 25.4 bits (54), Expect = 8.1
Identities = 15/36 (41%), Positives = 22/36 (60%)
Frame = +2
Query: 162 IAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKL 197
I +ND +K++ RLE AA+ + K +EE I KL
Sbjct: 650 IDPSNDQARKAIRRLE--PLAAEKREKMKEEMIAKL 751
>BP032174
Length = 522
Score = 25.4 bits (54), Expect = 8.1
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = -3
Query: 125 PQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGN 159
P+PP RPK+ P + +S+ RRR + GN
Sbjct: 511 PRPPQPRPKIPP--LLGRQSRLSRRRKARVPPQGN 413
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.131 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,391,912
Number of Sequences: 28460
Number of extensions: 41428
Number of successful extensions: 245
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 240
length of query: 221
length of database: 4,897,600
effective HSP length: 87
effective length of query: 134
effective length of database: 2,421,580
effective search space: 324491720
effective search space used: 324491720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC147000.5