BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147000.3 + phase: 0 /pseudo
(651 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18710 125 2e-29
TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, p... 72 2e-13
TC18571 similar to UP|Q9S762 (Q9S762) ATP phosphoribosyl transfe... 34 0.084
TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase ... 33 0.11
TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfa... 31 0.71
TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial... 31 0.71
TC16353 weakly similar to UP|HQGT_ARATH (Q9M156) Probable hydroq... 31 0.71
TC19044 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (16%) 30 0.93
TC17375 mitogen-activated kinase kinase kinase alpha [Lotus corn... 30 0.93
TC8967 similar to UP|FXGA_HUMAN (P55316) Forkhead box protein G1... 30 1.6
TC13356 weakly similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-... 29 2.1
AV780705 29 2.1
TC8746 similar to PIR|T06396|T06396 isoprenylated protein - soyb... 29 2.7
TC17922 29 2.7
BI418203 28 3.5
TC9275 similar to UP|Q9M4A9 (Q9M4A9) Beta-1,3 glucanase precurso... 28 3.5
AV778595 28 4.6
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 28 4.6
TC14616 28 4.6
BI418940 27 7.9
>TC18710
Length = 843
Score = 125 bits (315), Expect = 2e-29
Identities = 69/210 (32%), Positives = 114/210 (53%), Gaps = 3/210 (1%)
Frame = +2
Query: 38 GPNGISFNFIKRFWDLVKDDFMQFLVDFHRNGKLIKGVNSTFIALIPKVNNPQRLNDYMP 97
G +G++ F ++ D+VKD ++ F ++ +N T + LIPKV +P+ +N + P
Sbjct: 188 GMDGMNGLFYQKNGDVVKDSVT*AILAFLERAEIPNEINETLVTLIPKVPHPESINQFRP 367
Query: 98 ISLVGCLYKVLAKVLANRLRNVIGSVVSDSQSAFIKGN*ILDGILIANEVVDDARR---M 154
IS LYKV++K+ RL++ + ++S QS FI+G I D +LI E R +
Sbjct: 368 ISGCTFLYKVISKIFVARLKDAMPDLISPMQSGFIQGRQIQDNLLIVQEAFHAINRPGAL 547
Query: 155 DKELLLFRVDFEKAYDSVDLKYLDMVMVTMNFPTLWQKWISECIGTAKAAVLVNGCPMEE 214
+ + ++D KAYD V+ K+L+ ++ F T W K I + +NG +
Sbjct: 548 GRNHSIIKLDMNKAYDRVEWKFLESSLLAFGFSTNWVKMIMILVSGVSYNYKINGVVGPK 727
Query: 215 FSIERGLRQGDPLSPFLFLLAAEGFNVLMK 244
+RGLRQGDP SP+LFL E ++L++
Sbjct: 728 LLPQRGLRQGDPFSPYLFLFTMEVLSLLIQ 817
>TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, partial
(14%)
Length = 592
Score = 72.4 bits (176), Expect = 2e-13
Identities = 34/106 (32%), Positives = 67/106 (63%)
Frame = -3
Query: 20 SHEEVKQAVWDCDSFKSPGPNGISFNFIKRFWDLVKDDFMQFLVDFHRNGKLIKGVNSTF 79
S +E+K+AV+ K+PG +GI+ F ++ ++VK+D ++DF +G+L +N T
Sbjct: 326 STDEIKEAVFSMGDLKAPGMDGINGLFYQQNREIVKNDVNNAILDFFDHGELPVELNETL 147
Query: 80 IALIPKVNNPQRLNDYMPISLVGCLYKVLAKVLANRLRNVIGSVVS 125
+ LIPK+ + + + + PIS +YKV++K++ RL+ + +++S
Sbjct: 146 VTLIPKIPHAEAIQQFRPISCCSFIYKVISKIIVARLKPDMVNLIS 9
>TC18571 similar to UP|Q9S762 (Q9S762) ATP phosphoribosyl transferase ,
partial (11%)
Length = 434
Score = 33.9 bits (76), Expect = 0.084
Identities = 14/26 (53%), Positives = 15/26 (56%)
Frame = +2
Query: 552 WRWRRRVRWRRCGGWGGAVMEGSGFG 577
WRWRR V WR GG GG+ G G
Sbjct: 278 WRWRRCVGWRLIGGGGGSYGGSVGMG 355
>TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase like
protein (At4g14440), partial (75%)
Length = 910
Score = 33.5 bits (75), Expect = 0.11
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +1
Query: 556 RRVRWRRCGGWGGAVMEGSGFGDVVCLHGRRRVC 589
RR RWRR GWG ++ GSG+G C+ RVC
Sbjct: 565 RRRRWRRRCGWGRSLGGGSGWGK--CMQR*GRVC 660
>TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfalfa
{Medicago sativa;}, partial (93%)
Length = 811
Score = 30.8 bits (68), Expect = 0.71
Identities = 17/40 (42%), Positives = 24/40 (59%), Gaps = 3/40 (7%)
Frame = -1
Query: 530 YSGMTLG*GRFRCG*SFLGYLSWRWRRR--VRWRRCG-GW 566
++G G*GR + G + +G + W WRRR +R R G GW
Sbjct: 253 WTGFC*G*GRVQFGRAGVGLVGWWWRRRRGIRRRELGIGW 134
>TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial (10%)
Length = 575
Score = 30.8 bits (68), Expect = 0.71
Identities = 21/46 (45%), Positives = 23/46 (49%), Gaps = 10/46 (21%)
Frame = -2
Query: 552 WRWRRRVRWRR-----CGGW-----GGAVMEGSGFGDVVCLHGRRR 587
WR RRR+RWRR CG W GG V G+G V GR R
Sbjct: 328 WRMRRRMRWRRRRGGGCGPWRCGEKGGEV--GAGLCRRVHRQGRVR 197
>TC16353 weakly similar to UP|HQGT_ARATH (Q9M156) Probable hydroquinone
glucosyltransferase (Arbutin synthase) , partial (52%)
Length = 885
Score = 30.8 bits (68), Expect = 0.71
Identities = 15/23 (65%), Positives = 15/23 (65%)
Frame = -3
Query: 553 RWRRRVRWRRCGGWGGAVMEGSG 575
R RR VRWRR GG G EGSG
Sbjct: 388 RGRRGVRWRRGGGRRGGGTEGSG 320
>TC19044 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (16%)
Length = 620
Score = 30.4 bits (67), Expect = 0.93
Identities = 18/42 (42%), Positives = 24/42 (56%), Gaps = 1/42 (2%)
Frame = -1
Query: 407 FKKKKKLGGGGGV-VRIIGKWLGLIGIRFVYQRKKGGWGLGG 447
+++KKK GGGGGV +R++ W G G K G WG G
Sbjct: 437 WRRKKKSGGGGGVDLRLLRCWGGETG-------KLGRWGWCG 333
>TC17375 mitogen-activated kinase kinase kinase alpha [Lotus corniculatus
var. japonicus]
Length = 1422
Score = 30.4 bits (67), Expect = 0.93
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +2
Query: 314 IYLSLGCGICIELSKREASFCLFGLTYWRGFSKIEFLETCCRPYC 358
++ S C + E + R +FCLFG+ WR + I C + C
Sbjct: 101 VFTSEHCSVLWE*TGRRITFCLFGICLWRFYP*ITTRIWCIQGTC 235
>TC8967 similar to UP|FXGA_HUMAN (P55316) Forkhead box protein G1A
(Forkhead-related protein FKHL2) (Transcription factor
BF-2) (Brain factor 2) (BF2) (HFK2), partial (6%)
Length = 1032
Score = 29.6 bits (65), Expect = 1.6
Identities = 15/33 (45%), Positives = 17/33 (51%), Gaps = 3/33 (9%)
Frame = -3
Query: 552 WRWRRRVRWR---RCGGWGGAVMEGSGFGDVVC 581
W WRRR RWR R G W G + G G + C
Sbjct: 166 WWWRRRRRWRWRERLGIWRG-MRGGGAIGRLAC 71
>TC13356 weakly similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like
protein, partial (16%)
Length = 433
Score = 29.3 bits (64), Expect = 2.1
Identities = 15/26 (57%), Positives = 15/26 (57%)
Frame = -2
Query: 541 RCG*SFLGYLSWRWRRRVRWRRCGGW 566
RCG SF G RWRRR W R GW
Sbjct: 237 RCGCSFGG----RWRRRGWWGRRQGW 172
>AV780705
Length = 524
Score = 29.3 bits (64), Expect = 2.1
Identities = 25/65 (38%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = -3
Query: 417 GGVVRIIGKW--LGLIGIRFVYQRKKGGWGLGG*ELLIWHC*ENGVGRC**IKRGCGIEC 474
GG VR W L +G RK+G W G *EL * G C* +R CG
Sbjct: 255 GGKVRGKEPWNTLAQLGQNDP*VRKRGAWASGT*ELRTLPS*LGKPGVC*QTRRPCGYVS 76
Query: 475 *KLSM 479
* LS+
Sbjct: 75 *NLSI 61
>TC8746 similar to PIR|T06396|T06396 isoprenylated protein - soybean
(fragment) {Glycine max;}, partial (30%)
Length = 594
Score = 28.9 bits (63), Expect = 2.7
Identities = 11/16 (68%), Positives = 12/16 (74%), Gaps = 3/16 (18%)
Frame = +3
Query: 552 WRWR---RRVRWRRCG 564
WRWR R +RWRRCG
Sbjct: 546 WRWRHLLRLLRWRRCG 593
>TC17922
Length = 470
Score = 28.9 bits (63), Expect = 2.7
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3
Query: 553 RWRRRVRWRRCGG 565
RWRRR RW CGG
Sbjct: 72 RWRRRQRWSPCGG 34
>BI418203
Length = 681
Score = 28.5 bits (62), Expect = 3.5
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -1
Query: 553 RWRRRVRWRRCGGW 566
RWR R RW+RC W
Sbjct: 84 RWRLRNRWKRCRAW 43
>TC9275 similar to UP|Q9M4A9 (Q9M4A9) Beta-1,3 glucanase precursor ,
partial (39%)
Length = 545
Score = 28.5 bits (62), Expect = 3.5
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -2
Query: 553 RWRRRVRWRRCGGWGG 568
RWR R WRRCGG GG
Sbjct: 55 RWRGR--WRRCGGAGG 14
>AV778595
Length = 594
Score = 28.1 bits (61), Expect = 4.6
Identities = 12/17 (70%), Positives = 13/17 (75%)
Frame = -2
Query: 554 WRRRVRWRRCGGWGGAV 570
WRRR R RRCG GG+V
Sbjct: 353 WRRRRR*RRCGRSGGSV 303
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 28.1 bits (61), Expect = 4.6
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = -3
Query: 265 LTHLQFADDTLIIGEKSWLNVRSMRAVLMLFEEVSGLKVNFHKS 308
++HL FAD+ ++ ++ + VL +F+ +SGL N KS
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKS 242
>TC14616
Length = 1353
Score = 28.1 bits (61), Expect = 4.6
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -3
Query: 552 WRWRRRVRWRRCGGWG 567
W WRRR RW CGG G
Sbjct: 205 WLWRRRGRW-HCGGCG 161
>BI418940
Length = 550
Score = 27.3 bits (59), Expect = 7.9
Identities = 20/42 (47%), Positives = 22/42 (51%), Gaps = 2/42 (4%)
Frame = -3
Query: 548 GYLSWRW--RRRVRWRRCGGWGGAVMEGSGFGDVVCLHGRRR 587
G + W W RRR W +CGG GGA M S G GRRR
Sbjct: 272 GRVGWCWGCRRRRSW-KCGG-GGAEMGLSKLGMFSGGGGRRR 153
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.344 0.156 0.540
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,804,902
Number of Sequences: 28460
Number of extensions: 210913
Number of successful extensions: 2792
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 2634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2773
length of query: 651
length of database: 4,897,600
effective HSP length: 96
effective length of query: 555
effective length of database: 2,165,440
effective search space: 1201819200
effective search space used: 1201819200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147000.3