BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147000.12 + phase: 0
(136 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9673 UP|BAA31218 (BAA31218) Histone H3, complete 263 6e-72
TC8062 UP|BAA31218 (BAA31218) Histone H3, complete 263 6e-72
TC8063 UP|BAA31218 (BAA31218) Histone H3, complete 263 6e-72
TC17955 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 254 4e-69
TC14950 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 254 4e-69
TC19421 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 252 2e-68
TC16931 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 169 3e-61
TC8064 homologue to GB|AAB36496.1|488573|MSU09463 histone H3.2 p... 110 1e-25
AV413508 94 8e-21
BP037032 30 0.14
TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Ar... 25 3.6
TC9807 weakly similar to UP|ME11_ARATH (Q9SJL6) Membrin 11 (AtME... 25 3.6
BP082405 25 6.1
BP072254 24 7.9
TC16741 weakly similar to UP|Q9FNS9 (Q9FNS9) Fructan 1-exohydrol... 24 7.9
AV777860 24 7.9
TC10788 weakly similar to UP|Q8YG29 (Q8YG29) Cytochrome C-type b... 24 7.9
>TC9673 UP|BAA31218 (BAA31218) Histone H3, complete
Length = 690
Score = 263 bits (673), Expect = 6e-72
Identities = 135/136 (99%), Positives = 135/136 (99%)
Frame = +1
Query: 1 MARTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRK LATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE
Sbjct: 76 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 255
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 256 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 435
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 436 MPKDIQLARRIRGERA 483
>TC8062 UP|BAA31218 (BAA31218) Histone H3, complete
Length = 768
Score = 263 bits (673), Expect = 6e-72
Identities = 135/136 (99%), Positives = 135/136 (99%)
Frame = +3
Query: 1 MARTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRK LATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE
Sbjct: 51 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 230
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 231 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 410
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 411 MPKDIQLARRIRGERA 458
>TC8063 UP|BAA31218 (BAA31218) Histone H3, complete
Length = 723
Score = 263 bits (673), Expect = 6e-72
Identities = 135/136 (99%), Positives = 135/136 (99%)
Frame = +2
Query: 1 MARTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRK LATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE
Sbjct: 71 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 250
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 251 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 430
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 431 MPKDIQLARRIRGERA 478
>TC17955 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, complete
Length = 639
Score = 254 bits (649), Expect = 4e-69
Identities = 131/136 (96%), Positives = 132/136 (96%)
Frame = +1
Query: 1 MARTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRK LATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 58 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 237
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 238 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 417
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 418 MPKDIQLARRIRGERA 465
>TC14950 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, complete
Length = 641
Score = 254 bits (649), Expect = 4e-69
Identities = 131/136 (96%), Positives = 132/136 (96%)
Frame = +2
Query: 1 MARTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRK LATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 65 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 244
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 245 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 424
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 425 MPKDIQLARRIRGERA 472
>TC19421 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, complete
Length = 672
Score = 252 bits (643), Expect = 2e-68
Identities = 130/136 (95%), Positives = 131/136 (95%)
Frame = +3
Query: 1 MARTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRK LATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 93 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 272
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 273 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 452
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLAR IRGERA
Sbjct: 453 MPKDIQLARXIRGERA 500
>TC16931 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, partial (96%)
Length = 440
Score = 169 bits (428), Expect(2) = 3e-61
Identities = 86/90 (95%), Positives = 87/90 (96%)
Frame = +1
Query: 1 MARTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRK LATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 46 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 225
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAV 90
LLIRKLPFQRLVREIAQDFKTDLRFQS AV
Sbjct: 226 LLIRKLPFQRLVREIAQDFKTDLRFQSSAV 315
Score = 80.9 bits (198), Expect(2) = 3e-61
Identities = 40/40 (100%), Positives = 40/40 (100%)
Frame = +2
Query: 92 ALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRI 131
ALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRI
Sbjct: 320 ALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRI 439
>TC8064 homologue to GB|AAB36496.1|488573|MSU09463 histone H3.2 precursor
{Medicago sativa;} , partial (45%)
Length = 486
Score = 110 bits (274), Expect = 1e-25
Identities = 55/57 (96%), Positives = 57/57 (99%)
Frame = +1
Query: 80 KTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA 136
+TDLRFQSHAVLALQEAAEAYLVGLFEDT+LCAIHAKRVTIMPKDIQLARRIRGERA
Sbjct: 79 QTDLRFQSHAVLALQEAAEAYLVGLFEDTHLCAIHAKRVTIMPKDIQLARRIRGERA 249
>AV413508
Length = 265
Score = 94.0 bits (232), Expect = 8e-21
Identities = 54/87 (62%), Positives = 56/87 (64%), Gaps = 28/87 (32%)
Frame = +3
Query: 29 SAPTTGGVKKPHRYRPGTVALR----------------------------EIRKYQKSTE 60
SAPTTGGVKKPHRYRPGTVALR EIRKYQKSTE
Sbjct: 3 SAPTTGGVKKPHRYRPGTVALR*FTVFPQILSRFLSVAED*IFLFVYNFSEIRKYQKSTE 182
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQS 87
LLIRKLPFQRLVREIAQDFK L F++
Sbjct: 183LLIRKLPFQRLVREIAQDFKVILVFKT 263
>BP037032
Length = 577
Score = 30.0 bits (66), Expect = 0.14
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +1
Query: 23 TKAARKSAPTTGGVKK-PHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVRE 74
T+ R++ P G + PHR RP V + + + LIR LP R++R+
Sbjct: 142 TQRPRRTNPPPGAERHHPHRLRPRNVRVHTVSDQKPLGSELIRILPHVRILRQ 300
>TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Arabidopsis
thaliana;}, partial (52%)
Length = 800
Score = 25.4 bits (54), Expect = 3.6
Identities = 16/60 (26%), Positives = 27/60 (44%)
Frame = +1
Query: 3 RTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELL 62
R +Q R S + PR+PL + R T + P+R P +R +R + ++ L
Sbjct: 277 RRQQPRRDSPSSRHPRRPLESLRRRPPPSTLLERRSPNRLHP----VRHLRSRRHPSQAL 444
>TC9807 weakly similar to UP|ME11_ARATH (Q9SJL6) Membrin 11 (AtMEMB11)
(Golgi SNAP receptor complex member 2-1) (27 kDa Golgi
SNARE protein), partial (68%)
Length = 1062
Score = 25.4 bits (54), Expect = 3.6
Identities = 17/65 (26%), Positives = 29/65 (44%), Gaps = 9/65 (13%)
Frame = +2
Query: 10 KSTGGKAPRKPLATKAAR---KSAPTTGGVKKPHRYRPGTVALRE------IRKYQKSTE 60
K T + PR+P +AAR + +++ YR G + LR IR++Q
Sbjct: 131 KETSAQVPRRPRTPRAARALCRRRRLGAIIRREEGYRSGPIPLRRDGPPLGIRRWQTPAR 310
Query: 61 LLIRK 65
+ +K
Sbjct: 311 SVEKK 325
>BP082405
Length = 402
Score = 24.6 bits (52), Expect = 6.1
Identities = 14/41 (34%), Positives = 18/41 (43%)
Frame = +2
Query: 3 RTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKPHRYR 43
R K+ GG KP + KS GG +PHR+R
Sbjct: 203 RVKERRSDGDGGHCIIKP*PWRKTTKS----GGYNRPHRFR 313
>BP072254
Length = 419
Score = 24.3 bits (51), Expect = 7.9
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +2
Query: 3 RTKQTARKSTGGKAPRKPLATKAARKSAPTTGGVKKP 39
RTK K+T RK K AR ++PT G KP
Sbjct: 182 RTK*DFSKTTCKTHQRKTHQRKEARNTSPTGHGSAKP 292
>TC16741 weakly similar to UP|Q9FNS9 (Q9FNS9) Fructan 1-exohydrolase I
precursor, partial (25%)
Length = 680
Score = 24.3 bits (51), Expect = 7.9
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = -2
Query: 89 AVLALQEAAEAYLVGLFEDTNLCAIHAKRV 118
++L+L A Y+ +F NLC +HA +
Sbjct: 517 SLLSLSRLATFYIAYIFLIANLCFLHAPSI 428
>AV777860
Length = 558
Score = 24.3 bits (51), Expect = 7.9
Identities = 13/42 (30%), Positives = 23/42 (53%)
Frame = -3
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLV 102
LL+ K P +V +++ F++DL+ Q H + + YLV
Sbjct: 403 LLLVKKP*YTVVPMLSKLFQSDLQLQKHTEELILMVLDIYLV 278
>TC10788 weakly similar to UP|Q8YG29 (Q8YG29) Cytochrome C-type biogenesis
protein, partial (25%)
Length = 656
Score = 24.3 bits (51), Expect = 7.9
Identities = 12/32 (37%), Positives = 16/32 (49%)
Frame = +3
Query: 57 KSTELLIRKLPFQRLVREIAQDFKTDLRFQSH 88
K+ L+RK P +I K LRF+SH
Sbjct: 63 KTLNPLLRKFPLLNFPEKIQMASKLALRFRSH 158
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.133 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,537,361
Number of Sequences: 28460
Number of extensions: 14159
Number of successful extensions: 101
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of query: 136
length of database: 4,897,600
effective HSP length: 81
effective length of query: 55
effective length of database: 2,592,340
effective search space: 142578700
effective search space used: 142578700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Medicago: description of AC147000.12