BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146972.3 + phase: 0
(240 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8302 49 8e-07
BI418821 43 5e-05
TC9039 42 1e-04
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 40 5e-04
AV420911 38 0.001
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 37 0.002
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 36 0.005
AV773507 35 0.009
AV421607 33 0.044
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 32 0.097
BP065763 32 0.097
TC12773 30 0.48
TC17929 30 0.48
BP039068 30 0.48
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 29 0.63
TC9048 29 0.82
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 28 1.1
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 28 1.1
TC7836 homologue to UP|G3PA_PEA (P12858) Glyceraldehyde 3-phosph... 28 1.4
TC10378 similar to UP|Q84TG2 (Q84TG2) At3g16350, partial (26%) 27 4.1
>TC8302
Length = 494
Score = 48.9 bits (115), Expect = 8e-07
Identities = 36/138 (26%), Positives = 66/138 (47%), Gaps = 6/138 (4%)
Frame = +3
Query: 59 QQLYSFRMVESKAIMEQLTEFNKILDDLENIEVQLEDEDKAILLLCALPKSFE----SFK 114
++LY+ RM ES ++ + + N + L + + ++A LLL +LP S++ + K
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 115 NTMLYGKEGTVTLEEIQAALRTKELTNSKDL--THEHDEGLSVSRGNGGGRGNRRKSGNK 172
N + + L E+ + N +D + + E L+++RG R KS K
Sbjct: 183 NNNIVNHLPLMMLSEVLLE-EDSQR*NKEDR*DSSKPMEALTMTRG-------RSKSRKK 338
Query: 173 SRFECFNCHKMGHFKKDC 190
+C++C + H KKDC
Sbjct: 339 INLKCYHCGQR*HLKKDC 392
>BI418821
Length = 614
Score = 42.7 bits (99), Expect = 5e-05
Identities = 37/119 (31%), Positives = 45/119 (37%), Gaps = 9/119 (7%)
Frame = +2
Query: 127 LEEIQAALRTKELTNSKDLTHEHDEGL------SVSRGNGGGRGNRRKSGNKSRFE---C 177
L E A + T + D+T + L S RG GG RG R++G C
Sbjct: 215 LVEFSIATGDNDKTKAVDVTGPNGAALQPTRKDSAPRGFGGWRGGERRNGGGGGGGGGGC 394
Query: 178 FNCHKMGHFKKDCPEINGNSAQIVYEGYEDVGALMVWCCLEDEEGDVSHLGSDACNTPN 236
+NC GH +DC N N G GA C GD HL D CN N
Sbjct: 395 YNCGDTGHLARDCHRSNNN-------GGGGGGAACYNC------GDAGHLARD-CNRSN 529
Score = 36.2 bits (82), Expect = 0.005
Identities = 26/81 (32%), Positives = 29/81 (35%)
Frame = +2
Query: 156 SRGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPEINGNSAQIVYEGYEDVGALMVWC 215
S NGGG G C+NC GH +DC N NS G +
Sbjct: 440 SNNNGGGGGGAA---------CYNCGDAGHLARDCNRSNNNSGG---------GGAGCYN 565
Query: 216 CLEDEEGDVSHLGSDACNTPN 236
C GD HL D CN N
Sbjct: 566 C-----GDTGHLARD-CNRSN 610
>TC9039
Length = 1218
Score = 41.6 bits (96), Expect = 1e-04
Identities = 37/164 (22%), Positives = 60/164 (36%), Gaps = 8/164 (4%)
Frame = +2
Query: 35 AASMWAKLESLYMTKSLAHRQFLKQQLYSFRMVESKAIMEQLTEFNKILDDLENIEVQLE 94
A A++E + A L Q L S + I E + + I L+ ++++L
Sbjct: 395 AKDFLAEIEKRFAKSDKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELS 574
Query: 95 DEDKAILLLCALPKSFESFKNTMLYGKEGTVTLEEIQAALRTKELTNSKDLTHEHDEGLS 154
D+ L+L +LP F FK + KE E I ++ +E + H S
Sbjct: 575 DDLLIHLVLLSLPAQFSQFKISYNCPKEKWSLNELISFCVQEEERLKQERKESAHFVSTS 754
Query: 155 VSRGNGGGRGNRRKSG--------NKSRFECFNCHKMGHFKKDC 190
+G + K C+ C+ GH KK C
Sbjct: 755 KDKGKRKKTVEPKNEAADAPAPKKQKEDDTCYFCNVSGHMKKKC 886
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 39.7 bits (91), Expect = 5e-04
Identities = 23/69 (33%), Positives = 33/69 (47%), Gaps = 7/69 (10%)
Frame = +1
Query: 135 RTKELTNSKDLTHEHD--EGLSV-----SRGNGGGRGNRRKSGNKSRFECFNCHKMGHFK 187
R +E +D H D G V S+G GGGRG R G + +C+ C + GHF
Sbjct: 22 RERERERERDAIHALDGKNGWRVELSHNSKGGGGGRGGGRGRGGED-LKCYECGEPGHFA 198
Query: 188 KDCPEINGN 196
++C G+
Sbjct: 199 RECRSRGGS 225
>AV420911
Length = 418
Score = 38.1 bits (87), Expect = 0.001
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +2
Query: 156 SRGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDC 190
S+G GGGRG R G + +C+ C + GHF ++C
Sbjct: 314 SKGGGGGRGGGRGRGGED-LKCYECGEPGHFAREC 415
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 37.4 bits (85), Expect = 0.002
Identities = 27/94 (28%), Positives = 41/94 (42%), Gaps = 7/94 (7%)
Frame = +2
Query: 105 ALPKSFESFKNTMLYGKEGTVTLEEIQAALRTK-------ELTNSKDLTHEHDEGLSVSR 157
A+ ++ ++ L G+ TV + Q + R + E +D + D SR
Sbjct: 215 AMDEAIDAMNGMDLDGRTITVDKAQPQGSSRDRDDGDRYRERGRDRDDRGDRDR----SR 382
Query: 158 GNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCP 191
G GG RG+ ECF C K GHF ++CP
Sbjct: 383 GYGGSRGSNGG-------ECFKCGKPGHFARECP 463
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 36.2 bits (82), Expect = 0.005
Identities = 15/35 (42%), Positives = 20/35 (56%), Gaps = 2/35 (5%)
Frame = +1
Query: 158 GNGGGRGNRRKSGNK--SRFECFNCHKMGHFKKDC 190
G GGGRG R G S +C+ C + GHF ++C
Sbjct: 316 GGGGGRGGGRSGGGGGGSDMKCYECGEPGHFAREC 420
>AV773507
Length = 496
Score = 35.4 bits (80), Expect = 0.009
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = +2
Query: 150 DEGLSVSRGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPEINGNSAQ 199
D+G + GGRG+ + +CF C + GH +DCP G +
Sbjct: 269 DQGYRGGGYSSGGRGSYGAGDRVGQDDCFECGRPGHRARDCPLAGGGGGR 418
>AV421607
Length = 245
Score = 33.1 bits (74), Expect = 0.044
Identities = 15/40 (37%), Positives = 22/40 (54%)
Frame = +3
Query: 158 GNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPEINGNS 197
G+G G G SG+ + C+ C + GH+ +DCP NS
Sbjct: 9 GSGPGSG----SGSGTATGCYKCGRPGHWSRDCPSSAPNS 116
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 32.0 bits (71), Expect = 0.097
Identities = 24/81 (29%), Positives = 35/81 (42%), Gaps = 9/81 (11%)
Frame = +2
Query: 119 YGKEGTVTLEEIQAALRTKELTNSKDLTHEHD----EG--LSVSRGNGGGRGNRRKSGN- 171
YG+ V ++ A + + ++ D + D EG + V GG RGNR G
Sbjct: 170 YGRVRGVDMKHNFAFVEFSDPRDADDARYNLDGRDVEGSRIVVEFAKGGPRGNREYLGRG 349
Query: 172 --KSRFECFNCHKMGHFKKDC 190
CFNC GH+ +DC
Sbjct: 350 PPPGSGRCFNCGLDGHWARDC 412
>BP065763
Length = 537
Score = 32.0 bits (71), Expect = 0.097
Identities = 22/63 (34%), Positives = 30/63 (46%), Gaps = 7/63 (11%)
Frame = -1
Query: 137 KELTNSKDLTHEHDEGLSVSRGNGGGRGNRRKSGNKSR-------FECFNCHKMGHFKKD 189
KE +S LT HD G S S+ G + ++ K + C+ C K GHFKK
Sbjct: 516 KEKDHSIHLT-THD-GASXSKAKPGRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKY 343
Query: 190 CPE 192
CP+
Sbjct: 342 CPK 334
>TC12773
Length = 420
Score = 29.6 bits (65), Expect = 0.48
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 4/30 (13%)
Frame = +2
Query: 165 NRRKSGNK----SRFECFNCHKMGHFKKDC 190
NR + NK S +EC+ C + GH +DC
Sbjct: 299 NRHRWSNKRSSLSTYECWKCQRPGHMAEDC 388
>TC17929
Length = 791
Score = 29.6 bits (65), Expect = 0.48
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = +2
Query: 157 RGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPEINGNSAQI 200
R G + R + +K R C+ C + GH ++CP+ + + +I
Sbjct: 2 RAQIGEETSNRPNDSKFRQTCYRCGESGHKMRNCPKEHSSGEKI 133
>BP039068
Length = 467
Score = 29.6 bits (65), Expect = 0.48
Identities = 11/38 (28%), Positives = 16/38 (41%)
Frame = +3
Query: 156 SRGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPEI 193
+RG + N + C NC + GH DCP +
Sbjct: 249 ARGPTASTSSNVNHNNPRQTVCMNCQETGHASNDCPSL 362
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 29.3 bits (64), Expect = 0.63
Identities = 15/39 (38%), Positives = 19/39 (48%), Gaps = 4/39 (10%)
Frame = +1
Query: 156 SRGNGGGRGNRRKSGNKS----RFECFNCHKMGHFKKDC 190
SR GGGR R+ + CFNC GH+ +DC
Sbjct: 316 SREGGGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDC 432
>TC9048
Length = 599
Score = 28.9 bits (63), Expect = 0.82
Identities = 14/44 (31%), Positives = 24/44 (53%)
Frame = -1
Query: 190 CPEINGNSAQIVYEGYEDVGALMVWCCLEDEEGDVSHLGSDACN 233
C I+GN Q++Y+G++ V + CCL+ + + SD N
Sbjct: 335 CHFISGNIHQLIYKGHKGVATCI--CCLKS*QQQLLQPSSDRSN 210
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 28.5 bits (62), Expect = 1.1
Identities = 14/49 (28%), Positives = 19/49 (38%), Gaps = 3/49 (6%)
Frame = -2
Query: 148 EHDEGLSVSRGNGGGRGN---RRKSGNKSRFECFNCHKMGHFKKDCPEI 193
+ + S G GN R + S C C K GHF CP++
Sbjct: 425 QRPQNRGTSSGYSHSFGNFVPRPTQSDTSEIVCHRCSKKGHFANRCPDL 279
Score = 27.3 bits (59), Expect = 2.4
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -2
Query: 177 CFNCHKMGHFKKDC 190
C+NC K GH KDC
Sbjct: 275 CWNCQKTGHSGKDC 234
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 28.5 bits (62), Expect = 1.1
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2
Query: 177 CFNCHKMGHFKKDCPEI 193
C NC + GHF ++CP +
Sbjct: 344 CKNCKRPGHFARECPNV 394
>TC7836 homologue to UP|G3PA_PEA (P12858) Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A) ,
partial (89%)
Length = 1513
Score = 28.1 bits (61), Expect = 1.4
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -3
Query: 181 HKMGHFKKDCPEINGNSAQIVYEGYEDVGALMVWCC 216
+K+ H C GN+ ++ +EG+ED G + CC
Sbjct: 1031 NKVHHRNVWCRHTEGNAVELAFEGWEDKGHSL--CC 930
>TC10378 similar to UP|Q84TG2 (Q84TG2) At3g16350, partial (26%)
Length = 442
Score = 26.6 bits (57), Expect = 4.1
Identities = 14/43 (32%), Positives = 17/43 (38%)
Frame = +2
Query: 156 SRGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPEINGNSA 198
SRG G G G R C +C K H + CP G +
Sbjct: 62 SRGGGIGMGRR----------CSHCSKENHNSRTCPSRGGGGS 160
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,673,692
Number of Sequences: 28460
Number of extensions: 42896
Number of successful extensions: 293
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 291
length of query: 240
length of database: 4,897,600
effective HSP length: 88
effective length of query: 152
effective length of database: 2,393,120
effective search space: 363754240
effective search space used: 363754240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146972.3