BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146941.5 - phase: 0
(191 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15480 similar to UP|V712_ARATH (Q9SIQ9) Vesicle-associated mem... 207 7e-55
AV409249 115 4e-27
TC9745 similar to UP|V727_ARATH (Q9M376) Vesicle-associated memb... 102 6e-23
TC10105 similar to UP|Q7X9C5 (Q7X9C5) Synptobrevin-related prote... 88 1e-18
TC14440 similar to UP|V711_ARATH (O49377) Vesicle-associated mem... 74 1e-14
AV417281 71 1e-13
TC17245 similar to UP|V721_ARATH (Q9ZTW3) Vesicle-associated mem... 69 7e-13
TC13865 similar to UP|V714_ARATH (Q9FMR5) Vesicle-associated mem... 64 2e-11
TC14467 similar to UP|Q84SZ1 (Q84SZ1) Synaptobrevin-like protein... 62 6e-11
TC9344 similar to UP|AAQ84317 (AAQ84317) Fiber NTGP1-related pro... 62 8e-11
TC16775 similar to GB|AAA56991.1|600710|ATHSAR1 formerly called ... 49 6e-07
BG662131 46 5e-06
BI420754 32 0.054
TC9131 weakly similar to UP|O80986 (O80986) At2g26110 protein, p... 27 1.7
TC17758 similar to UP|V724_ARATH (O23429) Vesicle-associated mem... 27 1.7
TC12593 26 3.8
BP029912 26 3.8
CB826949 25 6.6
AV427033 25 8.6
>TC15480 similar to UP|V712_ARATH (Q9SIQ9) Vesicle-associated membrane
protein 712 (AtVAMP712), partial (53%)
Length = 482
Score = 207 bits (528), Expect = 7e-55
Identities = 99/118 (83%), Positives = 111/118 (93%)
Frame = +1
Query: 1 MGILYALVARGTVVLAEFTGTTTNAAAIARQILEKIPGNNDTHVSYSQDRYIFHVKRTDG 60
MGILYALVARG+V LAEF+GT TNA+ IA+QIL+KIPGNNDTHVSYSQDR+IFH+KRTDG
Sbjct: 127 MGILYALVARGSVALAEFSGTATNASTIAKQILDKIPGNNDTHVSYSQDRFIFHLKRTDG 306
Query: 61 LTVLCMADDSVGRRIPFAFLEEIHQRFVRSYGRAVLTAEAYAMNDEFSRVLNQQMEYF 118
LTVLCMADD+ GRRIPFAFLEEIHQRFVR+YGRAV +A+ Y MNDEFSRVL+QQMEYF
Sbjct: 307 LTVLCMADDNAGRRIPFAFLEEIHQRFVRTYGRAVFSAQPYGMNDEFSRVLSQQMEYF 480
>AV409249
Length = 286
Score = 115 bits (289), Expect = 4e-27
Identities = 53/82 (64%), Positives = 69/82 (83%)
Frame = +2
Query: 1 MGILYALVARGTVVLAEFTGTTTNAAAIARQILEKIPGNNDTHVSYSQDRYIFHVKRTDG 60
M ILYALVARGTVVLAEF+ T N A+AR+I+EK+P +D+ + +SQDRYIFH+ R+DG
Sbjct: 41 MAILYALVARGTVVLAEFSAVTGNTGAVARRIIEKLPEESDSRLCFSQDRYIFHILRSDG 220
Query: 61 LTVLCMADDSVGRRIPFAFLEE 82
LT LCMA+D+ GRRIPF++LE+
Sbjct: 221 LTYLCMANDTFGRRIPFSYLED 286
>TC9745 similar to UP|V727_ARATH (Q9M376) Vesicle-associated membrane
protein 727 (AtVAMP727), partial (70%)
Length = 721
Score = 102 bits (253), Expect = 6e-23
Identities = 56/166 (33%), Positives = 95/166 (56%), Gaps = 21/166 (12%)
Frame = +3
Query: 2 GILYALVARGTVVLAEFTGTTTNAAAIARQILEKIPGNNDTHVSYSQDRYIFHVKRTDGL 61
G++Y+ VA+GTVVLAE T T N + IA Q L+K+P N+ + +YS D + F+ +G
Sbjct: 225 GLIYSFVAKGTVVLAEHTQYTGNFSTIAVQCLQKLPSNSSKY-TYSCDGHTFNFLLDNGF 401
Query: 62 TVLCMADDSVGRRIPFAFLEEIHQRFVRSYGRAVLTAE--------------------AY 101
L +AD+SVGR +PF FLE + F++ YG ++ A AY
Sbjct: 402 VFLVVADESVGRSVPFVFLERVKDDFMQRYGASIKNASDHPLADDDDDDDLFQDRFSIAY 581
Query: 102 AMNDEFSRVLNQQMEYFSSDP-NADRINRLKGEMSQVRNVMIENID 146
++ EF L + M+Y + P ++++LK ++++V+ +M++NI+
Sbjct: 582 NLDREFGPALKEHMQYCLNHPEEMSKLSKLKAQITEVKGIMMDNIE 719
>TC10105 similar to UP|Q7X9C5 (Q7X9C5) Synptobrevin-related protein
(Fragment), partial (59%)
Length = 549
Score = 87.8 bits (216), Expect = 1e-18
Identities = 48/116 (41%), Positives = 72/116 (61%), Gaps = 1/116 (0%)
Frame = +1
Query: 3 ILYALVARGTVVLAEFTGTTTNAAAIARQILEKIPGNNDTHVSYSQDRYIFHVKRTDGLT 62
++YA V+RGTV+LAEFT + N +IA Q L+K+P ++ +Y+ D + F+ +G T
Sbjct: 205 LIYAFVSRGTVILAEFTEFSGNFNSIAFQCLQKLPSTSNKF-TYNCDAHTFNFLVDNGYT 381
Query: 63 VLCMADDSVGRRIPFAFLEEIHQRFVRSYGRA-VLTAEAYAMNDEFSRVLNQQMEY 117
+AD+SVGR++P AFLE + FV YG TA A ++N EF L + M+Y
Sbjct: 382 YCIVADESVGRQVPMAFLERVKDDFVSKYGDGKAATAPANSLNKEFGPKLKEHMQY 549
>TC14440 similar to UP|V711_ARATH (O49377) Vesicle-associated membrane
protein 711 (AtVAMP711) (v-SNARE synaptobrevin 7C)
(AtVAMP7C), partial (28%)
Length = 704
Score = 74.3 bits (181), Expect = 1e-14
Identities = 34/35 (97%), Positives = 34/35 (97%)
Frame = +1
Query: 157 LLVDKAANMQGNTFRFRKQARRFRSTVWWRNVKLT 191
LLVDK ANMQGNTFRFRKQARRFRSTVWWRNVKLT
Sbjct: 1 LLVDKTANMQGNTFRFRKQARRFRSTVWWRNVKLT 105
>AV417281
Length = 423
Score = 70.9 bits (172), Expect = 1e-13
Identities = 41/105 (39%), Positives = 60/105 (57%), Gaps = 1/105 (0%)
Frame = +3
Query: 3 ILYALVARGTVVLAEFTGTTTNAAAIARQILEKIPGNNDTHVSYSQDRYIFHVKRTDGLT 62
++Y+ VARGTV+LAE T N +A Q L+K+P +N T +Y+ D + F+ +G T
Sbjct: 111 LIYSFVARGTVILAEHTDFVGNFGDVALQFLQKLPSSN-TKFTYNTDGHTFNYLVHNGFT 287
Query: 63 VLCMADDSVGRRIPFAFLEEIHQRFVRSY-GRAVLTAEAYAMNDE 106
+A +S GR+I AFLE I + F + Y G TA +N E
Sbjct: 288 YCVVAVESTGRQIAMAFLERIKEDFTKRYDGGKAATAAPKGLNKE 422
>TC17245 similar to UP|V721_ARATH (Q9ZTW3) Vesicle-associated membrane
protein 721 (AtVAMP721) (v-SNARE synaptobrevin 7B)
(AtVAMP7B), partial (51%)
Length = 627
Score = 68.6 bits (166), Expect = 7e-13
Identities = 31/81 (38%), Positives = 52/81 (63%), Gaps = 1/81 (1%)
Frame = +2
Query: 111 LNQQMEYFSSDPN-ADRINRLKGEMSQVRNVMIENIDKVLDRGDRLELLVDKAANMQGNT 169
L + M+Y P ++ ++K ++S+V+ VM+ENI+KVLDRG+++ELLVDK N+
Sbjct: 122 LKEHMQYCVDHPEEVSKLAKVKAQVSEVKGVMMENIEKVLDRGEKIELLVDKTENLHHQA 301
Query: 170 FRFRKQARRFRSTVWWRNVKL 190
FR + R +W +N+K+
Sbjct: 302 QDFRNSGTKIRRKMWLQNMKI 364
>TC13865 similar to UP|V714_ARATH (Q9FMR5) Vesicle-associated membrane
protein 714 (AtVAMP714), partial (34%)
Length = 543
Score = 63.9 bits (154), Expect = 2e-11
Identities = 27/45 (60%), Positives = 36/45 (80%)
Frame = +2
Query: 146 DKVLDRGDRLELLVDKAANMQGNTFRFRKQARRFRSTVWWRNVKL 190
+K+L+RGDR+ELLVDK A MQ N+F FRKQ++R R +W +N KL
Sbjct: 2 EKILERGDRIELLVDKTATMQDNSFHFRKQSKRLRRALWMKNFKL 136
>TC14467 similar to UP|Q84SZ1 (Q84SZ1) Synaptobrevin-like protein, partial
(44%)
Length = 602
Score = 62.0 bits (149), Expect = 6e-11
Identities = 27/65 (41%), Positives = 44/65 (67%)
Frame = +2
Query: 126 RINRLKGEMSQVRNVMIENIDKVLDRGDRLELLVDKAANMQGNTFRFRKQARRFRSTVWW 185
++ ++K ++S+V+ VM+ENI+KVLDRG+++ELLVDK N+ FR R +W
Sbjct: 26 KLAKVKAQVSEVKGVMMENIEKVLDRGEKIELLVDKTENLHHQAQDFRTSGTSIRRKMWL 205
Query: 186 RNVKL 190
N+K+
Sbjct: 206 PNMKI 220
>TC9344 similar to UP|AAQ84317 (AAQ84317) Fiber NTGP1-related protein,
complete
Length = 995
Score = 61.6 bits (148), Expect = 8e-11
Identities = 36/151 (23%), Positives = 74/151 (48%)
Frame = +2
Query: 28 IARQILEKIPGNNDTHVSYSQDRYIFHVKRTDGLTVLCMADDSVGRRIPFAFLEEIHQRF 87
+ R + + P V + + Y H +GL L DD R F+ L ++ +
Sbjct: 173 VGRTVASRTPQGQRQSVQHEE--YKVHAYNRNGLCALGFMDDHYPVRSAFSLLNKVLDEY 346
Query: 88 VRSYGRAVLTAEAYAMNDEFSRVLNQQMEYFSSDPNADRINRLKGEMSQVRNVMIENIDK 147
+S+G + +A + LN + F AD++ +++ E+ + + ++ + ID
Sbjct: 347 QKSFGESWRNTQADSTQPW--PYLNDALTKFQDPAEADKLMKIQKELDETKIILHKTIDS 520
Query: 148 VLDRGDRLELLVDKAANMQGNTFRFRKQARR 178
VL RG++L+ LV+K++++ + F KQA++
Sbjct: 521 VLARGEKLDSLVEKSSDLSAASQMFYKQAKK 613
>TC16775 similar to GB|AAA56991.1|600710|ATHSAR1 formerly called HAT24;
synaptobrevin-related protein {Arabidopsis thaliana;} ,
partial (35%)
Length = 567
Score = 48.9 bits (115), Expect = 6e-07
Identities = 21/50 (42%), Positives = 34/50 (68%)
Frame = +2
Query: 141 MIENIDKVLDRGDRLELLVDKAANMQGNTFRFRKQARRFRSTVWWRNVKL 190
M+ENI+KVLDRG+++ELLVD+ ++ FR + R +W+ N+K+
Sbjct: 2 MMENIEKVLDRGEKIELLVDETDTLRSQAPDFRPPGTQIRRKMWFPNMKI 151
>BG662131
Length = 484
Score = 45.8 bits (107), Expect = 5e-06
Identities = 19/49 (38%), Positives = 34/49 (68%)
Frame = +3
Query: 142 IENIDKVLDRGDRLELLVDKAANMQGNTFRFRKQARRFRSTVWWRNVKL 190
I + + VLDRG+++ELLVDK ++Q F++Q R+ R +W +N+++
Sbjct: 3 IRHEENVLDRGEKIELLVDKTESLQFQADSFQRQGRQLRRKMWLQNLQM 149
>BI420754
Length = 581
Score = 32.3 bits (72), Expect = 0.054
Identities = 17/60 (28%), Positives = 27/60 (44%)
Frame = +2
Query: 39 NNDTHVSYSQDRYIFHVKRTDGLTVLCMADDSVGRRIPFAFLEEIHQRFVRSYGRAVLTA 98
N + +S Y FH + L M D + +++ F +LEE+ F R G + TA
Sbjct: 362 NEASRMSVESGPYFFHYIIEGRVCYLTMCDRAYPKKLAFQYLEELRNEFERVNGSQIETA 541
>TC9131 weakly similar to UP|O80986 (O80986) At2g26110 protein, partial
(21%)
Length = 781
Score = 27.3 bits (59), Expect = 1.7
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = -3
Query: 7 LVARGTVVLAEFTGTTTNAAAIARQILEKIP 37
L+A + L FTGT+ +A ++ +LEK+P
Sbjct: 158 LLADFFIFLDRFTGTSPDAGFVSELVLEKLP 66
>TC17758 similar to UP|V724_ARATH (O23429) Vesicle-associated membrane
protein 724 (AtVAMP724) (SYBL1-like protein), partial
(7%)
Length = 751
Score = 27.3 bits (59), Expect = 1.7
Identities = 11/24 (45%), Positives = 19/24 (78%)
Frame = +1
Query: 127 INRLKGEMSQVRNVMIENIDKVLD 150
+ RLK +S+V+++M+ N D+VLD
Sbjct: 388 LKRLKAPVSEVKSIML*NPDQVLD 459
>TC12593
Length = 846
Score = 26.2 bits (56), Expect = 3.8
Identities = 20/83 (24%), Positives = 36/83 (43%), Gaps = 16/83 (19%)
Frame = +3
Query: 121 DPNADRINRLKGEMSQVRNVMIENIDKVL----------------DRGDRLELLVDKAAN 164
D N DR + + +++R + EN+ ++ RGD L+ + +
Sbjct: 216 DRNRDRSSERRHSGARLRPELRENLGELQRRSLLPRQ*HHPHHRRQRGDNLQ*HRPREPD 395
Query: 165 MQGNTFRFRKQARRFRSTVWWRN 187
G+T R ++ RRF WW+N
Sbjct: 396 AAGDTERAKRPRRRF--VWWWQN 458
>BP029912
Length = 559
Score = 26.2 bits (56), Expect = 3.8
Identities = 24/103 (23%), Positives = 45/103 (43%), Gaps = 8/103 (7%)
Frame = -3
Query: 78 AFLEEIHQRFVRSYGRAVLTAEAYAMNDEFSRVLNQQMEYFSSD------PNADRINRLK 131
AFL +++ +GR+V AE M EF + M P R + L+
Sbjct: 494 AFLTQMNVLMNMMWGRSVEGAEGETMGAEFKEAVADMMTLIGKPNLSDFFPGLARFD-LQ 318
Query: 132 GEMSQVRNVMIENIDKVLDR--GDRLELLVDKAANMQGNTFRF 172
G Q++ V++ D++ D+ G+R+++ N + +F
Sbjct: 317 GVKKQMQAVVL-RFDRIFDKVIGERVKMEAKGKENESXDFLQF 192
>CB826949
Length = 525
Score = 25.4 bits (54), Expect = 6.6
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +2
Query: 146 DKVLDRGDRLELLVDKAANMQ 166
DK+LD +LE LVD+ AN Q
Sbjct: 296 DKILDVKQKLEELVDQPANNQ 358
>AV427033
Length = 420
Score = 25.0 bits (53), Expect = 8.6
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +2
Query: 177 RRFRSTVWWRN 187
RRFRST WW++
Sbjct: 119 RRFRSTSWWKS 151
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.136 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,265,738
Number of Sequences: 28460
Number of extensions: 20790
Number of successful extensions: 146
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of query: 191
length of database: 4,897,600
effective HSP length: 85
effective length of query: 106
effective length of database: 2,478,500
effective search space: 262721000
effective search space used: 262721000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146941.5