BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146941.18 + phase: 0 /pseudo/partial
(211 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19060 similar to UP|Q9SXK9 (Q9SXK9) Heat shock factor, partial... 162 3e-41
TC13752 similar to GB|CAB63803.1|6624618|ATH251868 heat shock fa... 160 1e-40
AV415736 117 2e-27
TC14009 similar to UP|HSF5_ARATH (Q9S7U5) Heat shock factor prot... 117 2e-27
TC18077 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial... 27 2.0
TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete 27 2.6
BP077250 27 3.4
TC8747 similar to PIR|T08407|T08407 clathrin coat assembly prote... 26 4.5
AV778022 26 5.8
AV428041 26 5.8
AV429101 25 7.6
TC14285 similar to UP|Q9AXU5 (Q9AXU5) Pathogen-inducible alpha-d... 25 7.6
BP042279 25 7.6
CB829460 25 10.0
TC19020 25 10.0
>TC19060 similar to UP|Q9SXK9 (Q9SXK9) Heat shock factor, partial (58%)
Length = 660
Score = 162 bits (411), Expect = 3e-41
Identities = 89/201 (44%), Positives = 125/201 (61%), Gaps = 26/201 (12%)
Frame = +1
Query: 18 SQKSVPAPFLTKTYQLVDDPLTDHIVSWSDDETTFVVWRPPEFARDLLPNFFKHNNFSSF 77
SQ+SVPAPFLTKTY++VDDP T+ ++SWS+ TFVVW+ +FA+DLLP++FKHNNFSSF
Sbjct: 22 SQRSVPAPFLTKTYEMVDDPATEDVISWSESGNTFVVWKHADFAKDLLPSYFKHNNFSSF 201
Query: 78 VRQLNTYGFKKVVADRWEFANDYFKKGAKHLLCEIHRRKTPQHYQQQYYEQSPQIFQP-- 135
VRQLNTYGF+K+V D+WEFAN+ FK+G + LL EI RRKT Q Q+P++ +
Sbjct: 202 VRQLNTYGFRKIVPDKWEFANENFKRGKRELLSEIKRRKT---VVAQPSAQAPEVGKSGN 372
Query: 136 -------DE---SICWIDSPLPSPKSNTDILTA--------------LSEDNQRLRRKNF 171
DE S SP + + TA LS +N++L+++N
Sbjct: 373 STSNSGGDEVGSSSTSTSSPGSKNPGSVETTTAPPNTLPPATLQCANLSSENEKLKKENE 552
Query: 172 MLLSELSHMKNLYNDIIYFIQ 192
L EL+ K +++ F++
Sbjct: 553 TLGWELARAKKQCEELVAFLR 615
>TC13752 similar to GB|CAB63803.1|6624618|ATH251868 heat shock factor 7
{Arabidopsis thaliana;} , partial (36%)
Length = 501
Score = 160 bits (406), Expect = 1e-40
Identities = 71/100 (71%), Positives = 85/100 (85%)
Frame = +3
Query: 17 ESQKSVPAPFLTKTYQLVDDPLTDHIVSWSDDETTFVVWRPPEFARDLLPNFFKHNNFSS 76
+SQ+++P PFLTKTYQLVDDP D ++SW+ D +F+VWRP EFARDLLP +FKHNNFSS
Sbjct: 51 DSQRAIPTPFLTKTYQLVDDPSVDDLISWNKDGNSFIVWRPAEFARDLLPKYFKHNNFSS 230
Query: 77 FVRQLNTYGFKKVVADRWEFANDYFKKGAKHLLCEIHRRK 116
FVRQLNTYGF+KVV DRWEFAND F++G K LL +I RRK
Sbjct: 231 FVRQLNTYGFRKVVPDRWEFANDGFRRGQKDLLRDIQRRK 350
>AV415736
Length = 214
Score = 117 bits (292), Expect = 2e-27
Identities = 48/71 (67%), Positives = 60/71 (83%)
Frame = +2
Query: 36 DPLTDHIVSWSDDETTFVVWRPPEFARDLLPNFFKHNNFSSFVRQLNTYGFKKVVADRWE 95
D D ++SW+DD +TF+VW P FARDLLP +FKHNNFSSFVRQLNTYGF+KV+ DRWE
Sbjct: 2 DQSIDDVISWNDDGSTFIVWNPTVFARDLLPKYFKHNNFSSFVRQLNTYGFRKVIPDRWE 181
Query: 96 FANDYFKKGAK 106
F+N++F+KG K
Sbjct: 182 FSNEFFRKGEK 214
>TC14009 similar to UP|HSF5_ARATH (Q9S7U5) Heat shock factor protein 5 (HSF
5) (Heat shock transcription factor 5) (HSTF 5), partial
(27%)
Length = 406
Score = 117 bits (292), Expect = 2e-27
Identities = 59/108 (54%), Positives = 72/108 (66%), Gaps = 1/108 (0%)
Frame = +1
Query: 18 SQKSVPAPFLTKTYQLVDDPLTDHIVSWSDDET-TFVVWRPPEFARDLLPNFFKHNNFSS 76
S S APFL K Y +V+D TD I+SW++ TFV+ F+ LLP +FKHNNF+S
Sbjct: 61 SGSSSVAPFLNKCYDMVEDDSTDSIISWTEPSGHTFVISDITAFSVTLLPTYFKHNNFAS 240
Query: 77 FVRQLNTYGFKKVVADRWEFANDYFKKGAKHLLCEIHRRKTPQHYQQQ 124
F+RQLN YGF+KV DRWEFAN+ F +G KHLL I RRK P QQ
Sbjct: 241 FIRQLNIYGFRKVDTDRWEFANENFVRGQKHLLKNIRRRKHPHVTDQQ 384
>TC18077 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial (51%)
Length = 995
Score = 27.3 bits (59), Expect = 2.0
Identities = 9/22 (40%), Positives = 14/22 (62%)
Frame = -1
Query: 108 LLCEIHRRKTPQHYQQQYYEQS 129
L+C HR+ P HY +Y+Q+
Sbjct: 977 LVCIFHRKCIPSHYNYLFYDQT 912
>TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete
Length = 999
Score = 26.9 bits (58), Expect = 2.6
Identities = 19/61 (31%), Positives = 30/61 (49%), Gaps = 5/61 (8%)
Frame = -3
Query: 120 HYQQQY-YEQSPQIFQPDESI--CWIDSPL--PSPKSNTDILTALSEDNQRLRRKNFMLL 174
H+ QY + SP DE++ C P P P + T I+T L + +++KNF L
Sbjct: 811 HFNTQYNFSCSPLAMCADEALPTCRYTLPYCKPDPTTLTIIITLLIN*TKEIKKKNFSTL 632
Query: 175 S 175
+
Sbjct: 631 T 629
>BP077250
Length = 511
Score = 26.6 bits (57), Expect = 3.4
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1
Query: 162 DNQRLRRKNFMLLSELSHMKNLYNDI 187
++ + RRKNF L ++HM L DI
Sbjct: 229 NHHQSRRKNFSALPRMNHMHKLLTDI 306
>TC8747 similar to PIR|T08407|T08407 clathrin coat assembly protein homolog
F18B3.140 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (19%)
Length = 605
Score = 26.2 bits (56), Expect = 4.5
Identities = 15/33 (45%), Positives = 18/33 (54%)
Frame = +3
Query: 151 SNTDILTALSEDNQRLRRKNFMLLSELSHMKNL 183
SNT L + S N R R NFM LSHM+ +
Sbjct: 126 SNTMNLVSKSVSNWRSR*SNFMRKDLLSHMRGV 224
>AV778022
Length = 554
Score = 25.8 bits (55), Expect = 5.8
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +2
Query: 36 DPLTDHIVSWSDDETTFVVWRPP 58
+P +H S D ++F VW PP
Sbjct: 179 EPRPNHAASCGGDASSFAVWLPP 247
>AV428041
Length = 331
Score = 25.8 bits (55), Expect = 5.8
Identities = 25/85 (29%), Positives = 33/85 (38%), Gaps = 11/85 (12%)
Frame = -2
Query: 112 IHRRKTPQHYQQ---------QYYEQSPQIFQPDESICWIDSPLPSPKSNTD--ILTALS 160
+H + HY Q Q QSPQI CW + S T +LTALS
Sbjct: 282 VHNLQHKVHYLQLQPKEVPLRQVVTQSPQILHFSSGTCWEFHAIFSSADQTPHRMLTALS 103
Query: 161 EDNQRLRRKNFMLLSELSHMKNLYN 185
+Q L ++SH+K N
Sbjct: 102 NLSQS------HL*HKISHLKTHQN 46
>AV429101
Length = 281
Score = 25.4 bits (54), Expect = 7.6
Identities = 12/37 (32%), Positives = 17/37 (45%)
Frame = +3
Query: 75 SSFVRQLNTYGFKKVVADRWEFANDYFKKGAKHLLCE 111
S FVR +K+ D WE N++ + LCE
Sbjct: 9 SFFVRYFEGLMYKEDSRDAWEVVNEFLMEQPFSCLCE 119
>TC14285 similar to UP|Q9AXU5 (Q9AXU5) Pathogen-inducible alpha-dioxygenase,
partial (97%)
Length = 2266
Score = 25.4 bits (54), Expect = 7.6
Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +1
Query: 33 LVDDPLTDHIVSWSDDET-TFVVWRPPEFARDLLPN 67
L+D + H+VS +W PEF RDL+P+
Sbjct: 1336 LLDIGVPVHLVSMGHQACGALELWNYPEFLRDLIPH 1443
>BP042279
Length = 252
Score = 25.4 bits (54), Expect = 7.6
Identities = 8/24 (33%), Positives = 17/24 (70%)
Frame = -3
Query: 171 FMLLSELSHMKNLYNDIIYFIQNH 194
+ L S L+ ++N+Y ++ F+Q+H
Sbjct: 226 YALFSSLARLQNMYCKVLKFLQSH 155
>CB829460
Length = 546
Score = 25.0 bits (53), Expect = 10.0
Identities = 9/33 (27%), Positives = 19/33 (57%)
Frame = +3
Query: 106 KHLLCEIHRRKTPQHYQQQYYEQSPQIFQPDES 138
+HLL + ++ Q YE++ ++F+ D+S
Sbjct: 189 RHLLDNLQKKLDESFLNMQLYEKALELFEDDQS 287
>TC19020
Length = 438
Score = 25.0 bits (53), Expect = 10.0
Identities = 11/30 (36%), Positives = 16/30 (52%)
Frame = -2
Query: 172 MLLSELSHMKNLYNDIIYFIQNHVSPASPF 201
+L+ EL + N+YN +YF Q PF
Sbjct: 359 LLIHELQNNMNIYNMSLYFRQTSSEKPLPF 270
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.134 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,110,209
Number of Sequences: 28460
Number of extensions: 62014
Number of successful extensions: 317
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 315
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 315
length of query: 211
length of database: 4,897,600
effective HSP length: 86
effective length of query: 125
effective length of database: 2,450,040
effective search space: 306255000
effective search space used: 306255000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146941.18