BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146914.10 + phase: 0
(541 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV424352 102 2e-22
AV409252 92 3e-19
AV410178 77 9e-15
AI967845 66 2e-11
BP032490 57 8e-09
AV765293 43 1e-04
TC17811 similar to UP|Q9FKL6 (Q9FKL6) Calmodulin-binding protein... 36 0.014
BP059446 32 0.34
TC14289 similar to UP|LEU1_SOYBN (Q39891) Probable 2-isopropylma... 30 0.99
TC8853 similar to PIR|T09217|T09217 protein sam2B - spinach {Spi... 28 2.9
TC14291 similar to UP|LEU1_SOYBN (Q39891) Probable 2-isopropylma... 28 2.9
TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich... 28 3.8
TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%) 27 6.4
TC9210 27 6.4
AV409964 27 6.4
CB826772 27 8.4
>AV424352
Length = 432
Score = 102 bits (254), Expect = 2e-22
Identities = 49/64 (76%), Positives = 56/64 (86%)
Frame = +3
Query: 269 ILGTGMSAKMWEVTVEHARTCVLDTTRHVSFASHSQQPHVVFNAVGEVTGLLAESEYVAV 328
ILGTGMSAKMWEVT+EHARTCVLD TR++ F +HSQQ VVFNAVG+VTGL++E EY V
Sbjct: 240 ILGTGMSAKMWEVTLEHARTCVLDMTRNLYFPAHSQQTGVVFNAVGQVTGLISEGEYADV 419
Query: 329 DKLS 332
DKLS
Sbjct: 420 DKLS 431
>AV409252
Length = 423
Score = 91.7 bits (226), Expect = 3e-19
Identities = 56/141 (39%), Positives = 81/141 (56%), Gaps = 2/141 (1%)
Frame = +2
Query: 66 LQLQFENSISLPVFTGARIEGEDGSNLRIRLVDALTGKVVCTGPESSAKVEIVVLEGDFE 125
LQ F N + +FT I+ E G L+I L DA + V SS ++ I VL+GDF
Sbjct: 2 LQFCFVNQLPETIFTQCDIKAEGGLALQIELRDAANQQSVVKDEGSSMRIRICVLDGDFG 181
Query: 126 EESDIWMPEDFKNNIVRERDGKKPLLTGDVILYLKDGLCMVG-EISYTDNSSWTRSRRFR 184
E W E+F I+ R+ K PLL GD ++ LK+G+ + ++ +TDNSS TRS +FR
Sbjct: 182 SED--WTAEEFNAQILSPRERKGPLLVGDTVITLKNGVGYISKKMVFTDNSSGTRSGKFR 355
Query: 185 LGVRVV-DNFDGIRIREAKTD 204
LGV++V N IRE +++
Sbjct: 356 LGVKIVQSNSSRTDIREGRSE 418
>AV410178
Length = 430
Score = 76.6 bits (187), Expect = 9e-15
Identities = 34/73 (46%), Positives = 52/73 (70%)
Frame = +1
Query: 267 RTILGTGMSAKMWEVTVEHARTCVLDTTRHVSFASHSQQPHVVFNAVGEVTGLLAESEYV 326
R ILG+GMS KMW++ VEHA+TCVL +V +A ++ VVFN + E++GL+A +Y
Sbjct: 1 RNILGSGMSNKMWDILVEHAKTCVLSGKLYVYYADDARNIGVVFNNIYELSGLIANDQYY 180
Query: 327 AVDKLSETEKAFI 339
+ D LSE++K ++
Sbjct: 181 SADTLSESQKVYV 219
>AI967845
Length = 324
Score = 65.9 bits (159), Expect = 2e-11
Identities = 32/79 (40%), Positives = 48/79 (60%), Gaps = 1/79 (1%)
Frame = +2
Query: 94 IRLVDALTGKVVCTGPESSAKVEIVVLEGDFEEE-SDIWMPEDFKNNIVRERDGKKPLLT 152
I L D + V GP SS K+EI L GDF ++ W +F +NI+ +RDGK+PLL
Sbjct: 17 IALFDVKSQSVFSDGPNSSIKIEICALNGDFGSSGNEDWTEVEFNDNILHQRDGKQPLLI 196
Query: 153 GDVILYLKDGLCMVGEISY 171
G+ + LK+G+C + +I +
Sbjct: 197 GERFITLKNGVCCIPKIGF 253
Score = 36.2 bits (82), Expect = 0.014
Identities = 16/35 (45%), Positives = 24/35 (67%)
Frame = +3
Query: 156 ILYLKDGLCMVGEISYTDNSSWTRSRRFRLGVRVV 190
+L LK +++++DNS W RSR+FRLG +VV
Sbjct: 207 LLLLKMEFVASLKLAFSDNSRWVRSRKFRLGAKVV 311
>BP032490
Length = 421
Score = 57.0 bits (136), Expect = 8e-09
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = -3
Query: 503 FMPVSSTSMTKGKAQRRWRKVVNVLKWFMVKKRRNQLYR 541
FM +T++ GKAQRRWRK+ NVLKWFMV+KR N YR
Sbjct: 419 FMVARTTTVAHGKAQRRWRKLFNVLKWFMVRKRPNPRYR 303
>AV765293
Length = 361
Score = 43.1 bits (100), Expect = 1e-04
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = -1
Query: 517 QRRWRKVVNVLKWFMVKKRRNQLYR 541
QR+WRK+ NVLKWFMV+KR NQ R
Sbjct: 361 QRKWRKLFNVLKWFMVRKRPNQKNR 287
>TC17811 similar to UP|Q9FKL6 (Q9FKL6) Calmodulin-binding protein, partial
(10%)
Length = 566
Score = 36.2 bits (82), Expect = 0.014
Identities = 20/38 (52%), Positives = 27/38 (70%)
Frame = +1
Query: 8 SVVREVMKLQSVRNLMEPILEPLVRRVVREEVELALKK 45
SV+ E +K+ S++ L LEP++RRVV EEVE AL K
Sbjct: 388 SVIVEALKVDSLQKLCSS-LEPILRRVVSEEVERALAK 498
>BP059446
Length = 542
Score = 31.6 bits (70), Expect = 0.34
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = +2
Query: 274 MSAKMWEVTVEHARTC 289
MS +MWEVT++HA+TC
Sbjct: 2 MSERMWEVTMKHAKTC 49
>TC14289 similar to UP|LEU1_SOYBN (Q39891) Probable 2-isopropylmalate synthase
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
(Late nodulin 56) (N-56) , partial (85%)
Length = 1822
Score = 30.0 bits (66), Expect = 0.99
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -1
Query: 131 WMPEDFKNNIVRERDGKKPLLTGDVILYL 159
W P F N IV D +P+ +GD+I Y+
Sbjct: 1162 WRPLSFPNTIVSPTDLTRPISSGDIISYV 1076
>TC8853 similar to PIR|T09217|T09217 protein sam2B - spinach {Spinacia
oleracea;}, partial (43%)
Length = 1157
Score = 28.5 bits (62), Expect = 2.9
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 510 SMTKGKAQRRWRKVVNVLKWFMVKKRRNQL 539
S+ +G++ RRWR+ + MVKK R +L
Sbjct: 310 SLRRGESWRRWRRSCRIRLMMMVKKVRRRL 399
>TC14291 similar to UP|LEU1_SOYBN (Q39891) Probable 2-isopropylmalate
synthase (Alpha-isopropylmalate synthase) (Alpha-IPM
synthetase) (Late nodulin 56) (N-56) , partial (40%)
Length = 902
Score = 28.5 bits (62), Expect = 2.9
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = -2
Query: 131 WMPEDFKNNIVRERDGKKPLLTGDVILYL 159
W P F + IV D +P+ +GD+I Y+
Sbjct: 442 WRPLSFPSTIVAPTDLSRPISSGDIISYV 356
>TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich protein 1
(NDPP-1). {Mus musculus;}, partial (8%)
Length = 1134
Score = 28.1 bits (61), Expect = 3.8
Identities = 21/88 (23%), Positives = 31/88 (34%)
Frame = +2
Query: 221 PPSLSDEVWRLEKIGKDGAFHRRLSREKIRTVKDFLTLLNLDPAKLRTILGTGMSAKMWE 280
PPS+S + A ++ +S +I V TLL P I G + +W
Sbjct: 239 PPSISRALLHYASFNSTTAANKAMSPAEINAVAT--TLLRTPPRPNLLIFGLTHESLLWS 412
Query: 281 VTVEHARTCVLDTTRHVSFASHSQQPHV 308
+ RT LD + P V
Sbjct: 413 ALNHNGRTVFLDENEYAISKFEQSNPGV 496
>TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%)
Length = 519
Score = 27.3 bits (59), Expect = 6.4
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +3
Query: 230 RLEKIGKDGAFHRRLSREKIRTVKDFLTLLNLD 262
+L+ G+D RRL R +I VK LT NL+
Sbjct: 309 QLKFFGEDHRVKRRLGRPRISCVKSILTCSNLN 407
>TC9210
Length = 944
Score = 27.3 bits (59), Expect = 6.4
Identities = 27/108 (25%), Positives = 49/108 (45%), Gaps = 11/108 (10%)
Frame = -1
Query: 327 AVDKLSETEKAFISDHPL--VSLADAQISVISALNQCDFASFEDEVSLMDGYSHLTNVH- 383
A+D ++ + + S++PL +S + IS S+ +CDF S L++ SH+ H
Sbjct: 836 ALDTIASSNRRTRSENPLKLLSFSVILISKRSSRKRCDFHSEMRSWELIEKSSHVIATHT 657
Query: 384 -----YSPSSPRTEGSSANKLLAL--QKTGGFNYTQESASSTD-IMPS 423
+ P S NK++ + + GF T +D ++PS
Sbjct: 656 FSMSTFPPDLATLVRGSINKVVII*Q*RCNGFKKTSPLRLGSDSVVPS 513
>AV409964
Length = 434
Score = 27.3 bits (59), Expect = 6.4
Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 4/59 (6%)
Frame = -1
Query: 279 WEVTVEHARTCVL----DTTRHVSFASHSQQPHVVFNAVGEVTGLLAESEYVAVDKLSE 333
W++ ++ + + L DT H+ HS+QPH+ F TG + E V V++ S+
Sbjct: 209 WKILIQTSNSVRLPSSHDTASHIP---HSRQPHIPFTLAHRTTGSI---EGVWVNRTSQ 51
>CB826772
Length = 490
Score = 26.9 bits (58), Expect = 8.4
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -2
Query: 46 HLSSIKQTCGKEMNTSESRTLQLQFENSISLPVFT 80
H S++ +E S R +Q E SISLP+FT
Sbjct: 120 HHSNLITVNSRENGGSHHRDTGIQVEPSISLPIFT 16
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.134 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,832,542
Number of Sequences: 28460
Number of extensions: 111222
Number of successful extensions: 507
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 541
length of database: 4,897,600
effective HSP length: 95
effective length of query: 446
effective length of database: 2,193,900
effective search space: 978479400
effective search space used: 978479400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146914.10