BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146862.7 - phase: 0
(198 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9601 284 6e-78
TC19964 similar to UP|Q40363 (Q40363) NuM1 protein, partial (11%) 30 0.28
AV413495 30 0.28
TC11268 similar to UP|Q7SBH0 (Q7SBH0) Predicted protein, partial... 29 0.47
TC11023 28 0.81
TC19228 28 1.1
TC14886 similar to GB|AAM10290.1|20147147|AY091691 AT3g13930/MDC... 27 1.8
TC9947 similar to UP|Q9FZF0 (Q9FZF0) T2E6.19, partial (40%) 27 1.8
TC17679 27 1.8
BP030309 27 2.4
TC17281 similar to AAQ89644 (AAQ89644) At4g17310, partial (40%) 27 2.4
AV778875 27 3.1
TC10388 similar to UP|Q84L20 (Q84L20) L-galactose dehydrogenase,... 27 3.1
AV778604 27 3.1
TC10717 similar to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A lig... 27 3.1
TC10727 weakly similar to UP|Q15287 (Q15287) RNA-binding protein... 27 3.1
TC14842 similar to UP|RK4_TOBAC (O80361) 50S ribosomal protein L... 26 4.0
AV775543 26 4.0
TC10327 weakly similar to UP|NLT1_PRUDU (Q43017) Nonspecific lip... 26 5.2
AV775297 26 5.2
>TC9601
Length = 588
Score = 284 bits (727), Expect = 6e-78
Identities = 130/163 (79%), Positives = 143/163 (86%)
Frame = +3
Query: 1 MQITCEASLKCLQGKGPPFTFQCNGSSMEVFPELNNEPGNHPSGNVPEPNHRLGSEFLEP 60
MQITCEASLKCLQGKG P TFQCNGSS+ EL N+PG HP+G+V EPN RLGSEFLEP
Sbjct: 108 MQITCEASLKCLQGKGFPSTFQCNGSSIT---ELKNDPGTHPAGDVSEPNRRLGSEFLEP 278
Query: 61 SNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPVENQFQYAPINMVSQGY 120
SNEFH+KPTYHH+Y+TWT+CHFH K+ QC MNAFE+HFYP+PVENQ QY P NM +Q Y
Sbjct: 279 SNEFHSKPTYHHEYNTWTSCHFHSPKVHQCPMNAFESHFYPYPVENQLQYVPANMFAQSY 458
Query: 121 PREQYQEFQNFVVIDFEATCDKDKNPHPQEIIEFPSVIVSSVT 163
PREQ+QEFQ FVVIDFEATCDKDKNPHPQEIIEFPSVIVSSVT
Sbjct: 459 PREQFQEFQYFVVIDFEATCDKDKNPHPQEIIEFPSVIVSSVT 587
>TC19964 similar to UP|Q40363 (Q40363) NuM1 protein, partial (11%)
Length = 562
Score = 30.0 bits (66), Expect = 0.28
Identities = 19/70 (27%), Positives = 28/70 (39%), Gaps = 12/70 (17%)
Frame = -3
Query: 45 NVPEPNHRLGSEFLEPSNEFHNKPTY-----------HHDYSTWTA-CHFHPHKMQQCQM 92
N+P P+ + S +L FH+ P + HH + T CH H H CQ+
Sbjct: 356 NIPSPHQQK*SSYLPLLRVFHDHPFHRHRFHLGHLGPHHGHRYQTGLCHHHVH----CQI 189
Query: 93 NAFENHFYPH 102
H + H
Sbjct: 188 GLHVRHHFFH 159
>AV413495
Length = 338
Score = 30.0 bits (66), Expect = 0.28
Identities = 14/54 (25%), Positives = 21/54 (37%), Gaps = 3/54 (5%)
Frame = -1
Query: 52 RLGSEFLEPSNEFHNKPTYHHDYSTWTACHFH---PHKMQQCQMNAFENHFYPH 102
++ F P+ + H P YHH S+ H H H + +HF H
Sbjct: 254 KVQDRFYSPTRDSHYHPVYHHQLSSPKRDHLHVLFSHLHEMIHQQTGHHHFSVH 93
>TC11268 similar to UP|Q7SBH0 (Q7SBH0) Predicted protein, partial (5%)
Length = 1097
Score = 29.3 bits (64), Expect = 0.47
Identities = 18/84 (21%), Positives = 29/84 (34%)
Frame = -1
Query: 41 HPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFY 100
H + PE + S+ PSN H H + H HPH + F
Sbjct: 851 HKTQKNPENKKSISSQTHSPSNTLHKNINLLHPHDLNHLHHLHPHHPLNNTKSPFFLKHQ 672
Query: 101 PHPVENQFQYAPINMVSQGYPREQ 124
N F ++ + + + PR +
Sbjct: 671 STTTPNPFHFSFHSEIERPQPRSE 600
>TC11023
Length = 735
Score = 28.5 bits (62), Expect = 0.81
Identities = 14/33 (42%), Positives = 16/33 (48%)
Frame = -1
Query: 69 TYHHDYSTWTACHFHPHKMQQCQMNAFENHFYP 101
T+HH HF PHK N F +HFYP
Sbjct: 570 TFHH--------HFFPHK--STSSNFFHDHFYP 502
>TC19228
Length = 589
Score = 28.1 bits (61), Expect = 1.1
Identities = 15/33 (45%), Positives = 18/33 (54%), Gaps = 3/33 (9%)
Frame = +2
Query: 47 PEPNHRLGSEFLEPSN---EFHNKPTYHHDYST 76
P PNHRL S L+PS+ F +P H ST
Sbjct: 80 PTPNHRLRSYALDPSSSLLHFSQQPRTHRTPST 178
>TC14886 similar to GB|AAM10290.1|20147147|AY091691 AT3g13930/MDC16_5
{Arabidopsis thaliana;}, partial (47%)
Length = 1352
Score = 27.3 bits (59), Expect = 1.8
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -2
Query: 82 FHPHKMQQCQMNAFENHFYPHPVENQFQ 109
+HPH Q Q++ YPHP E +Q
Sbjct: 679 YHPHLQQLLQLSHQLEFLYPHPHELSWQ 596
>TC9947 similar to UP|Q9FZF0 (Q9FZF0) T2E6.19, partial (40%)
Length = 560
Score = 27.3 bits (59), Expect = 1.8
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -3
Query: 53 LGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNA 94
L E L S+ + ++P H+ +W + H H K+ CQ+++
Sbjct: 324 LHHELLHQSDIYQDQPNAHNH--SWVSSHTHSDKLVFCQVHS 205
>TC17679
Length = 585
Score = 27.3 bits (59), Expect = 1.8
Identities = 22/95 (23%), Positives = 32/95 (33%), Gaps = 8/95 (8%)
Frame = +3
Query: 17 PPFTFQCNGSSMEVFPELNNEPGNHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYS- 75
PP T + ++ + P L N P HP P P +E EP + HD++
Sbjct: 285 PPSTSPSSTNTNPLQPALLN-PNTHPPATSPSPAPPPPTETPEPELAGEEEGRAEHDHNP 461
Query: 76 -------TWTACHFHPHKMQQCQMNAFENHFYPHP 103
T H P + +PHP
Sbjct: 462 D*FENPLTQNQTHLSPPSTENQPQTHRSKQTHPHP 566
>BP030309
Length = 490
Score = 26.9 bits (58), Expect = 2.4
Identities = 11/24 (45%), Positives = 13/24 (53%)
Frame = +2
Query: 49 PNHRLGSEFLEPSNEFHNKPTYHH 72
PNH S F S HN+P +HH
Sbjct: 224 PNHSSSSSF-SSSQHHHNRPCHHH 292
>TC17281 similar to AAQ89644 (AAQ89644) At4g17310, partial (40%)
Length = 431
Score = 26.9 bits (58), Expect = 2.4
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +2
Query: 14 GKGPPFTFQCNGSSMEVFPELNNEP 38
G GPP T CNG S+++ +N P
Sbjct: 2 GYGPPSTEICNGFSLQIILATSNVP 76
>AV778875
Length = 464
Score = 26.6 bits (57), Expect = 3.1
Identities = 8/29 (27%), Positives = 14/29 (47%)
Frame = -2
Query: 60 PSNEFHNKPTYHHDYSTWTACHFHPHKMQ 88
P+ + H P YHH +W H+++
Sbjct: 229 PATKHHTPPIYHHQKPSWVPATSTEHQLK 143
>TC10388 similar to UP|Q84L20 (Q84L20) L-galactose dehydrogenase, partial
(44%)
Length = 466
Score = 26.6 bits (57), Expect = 3.1
Identities = 11/37 (29%), Positives = 16/37 (42%)
Frame = +3
Query: 36 NEPGNHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHH 72
+EP + + P P ++ F PSN T HH
Sbjct: 90 SEPPLSATSSAPSPKNKPSPPFASPSNPASTSSTLHH 200
>AV778604
Length = 545
Score = 26.6 bits (57), Expect = 3.1
Identities = 21/81 (25%), Positives = 33/81 (39%), Gaps = 8/81 (9%)
Frame = +3
Query: 37 EPGNHPSGNVPEPNHRLGSEFLE-----PSNEFHNKPTYHH---DYSTWTACHFHPHKMQ 88
+P HP PEP + + P++ H KP HH D S ++ H H Q
Sbjct: 30 KPRTHPPPRHPEPTTTTKQKHINHPPPSPTSSRHPKPRTHHHP*DPSNQSSDH---HIHQ 200
Query: 89 QCQMNAFENHFYPHPVENQFQ 109
+++ H + +N FQ
Sbjct: 201 *AKLDI*IGHHHQLKADNPFQ 263
>TC10717 similar to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A ligase ,
partial (32%)
Length = 691
Score = 26.6 bits (57), Expect = 3.1
Identities = 12/38 (31%), Positives = 18/38 (46%)
Frame = +1
Query: 66 NKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHP 103
N +HH +S H H HKM+ +A + +P P
Sbjct: 49 NNYIHHHLHSPACLLHHHHHKMKNNSSSAPNSPTFPSP 162
>TC10727 weakly similar to UP|Q15287 (Q15287) RNA-binding protein
(RNA-binding protein S1, serine-rich domain), partial
(15%)
Length = 932
Score = 26.6 bits (57), Expect = 3.1
Identities = 13/39 (33%), Positives = 16/39 (40%)
Frame = +1
Query: 65 HNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHP 103
H +HH +T TA H PH+ M H P P
Sbjct: 7 HGLRRHHHHITTTTAIHLQPHRHSPL-MARVPRHLRPLP 120
>TC14842 similar to UP|RK4_TOBAC (O80361) 50S ribosomal protein L4,
chloroplast precursor (R-protein L4), partial (51%)
Length = 618
Score = 26.2 bits (56), Expect = 4.0
Identities = 17/60 (28%), Positives = 22/60 (36%)
Frame = +2
Query: 9 LKCLQGKGPPFTFQCNGSSMEVFPELNNEPGNHPSGNVPEPNHRLGSEFLEPSNEFHNKP 68
L+ LQ + PP + S V P N P H + P P R P + H P
Sbjct: 188 LRRLQARNPPGSLLHRRESRRVHPRPQNRPTKHRTCRRP-PRRRHRPPKQAPRHRLHQDP 364
>AV775543
Length = 355
Score = 26.2 bits (56), Expect = 4.0
Identities = 16/51 (31%), Positives = 22/51 (42%), Gaps = 9/51 (17%)
Frame = -1
Query: 68 PTYHHDYS---------TWTACHFHPHKMQQCQMNAFENHFYPHPVENQFQ 109
P HDY+ T ACH ++M+ CQ+ H + HP Q Q
Sbjct: 214 PLGDHDYAHVHP*PTPQTDVACH---NQMEYCQVRELLLHHHAHPQNRQKQ 71
>TC10327 weakly similar to UP|NLT1_PRUDU (Q43017) Nonspecific lipid-transfer
protein 1 precursor (LTP 1), partial (52%)
Length = 683
Score = 25.8 bits (55), Expect = 5.2
Identities = 15/61 (24%), Positives = 28/61 (45%)
Frame = -3
Query: 38 PGNHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFEN 97
PG+H + H++G+ +++ HN T H +S + + H H+ CQ +
Sbjct: 237 PGHHVA-----TIHQVGNAWVQA---VHNLTTCHSTFSPRNSGYRHEHREHNCQRDKPSY 82
Query: 98 H 98
H
Sbjct: 81 H 79
>AV775297
Length = 456
Score = 25.8 bits (55), Expect = 5.2
Identities = 19/61 (31%), Positives = 22/61 (35%)
Frame = +2
Query: 34 LNNEPGNHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMN 93
L GN S E RL EPS P H D TW + H H + Q +
Sbjct: 239 LPESAGNAISKAFKEAPKRLQGHQKEPSRG----PYPHRDRGTWRSAHTHQTQAQSAGVP 406
Query: 94 A 94
A
Sbjct: 407 A 409
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,979,685
Number of Sequences: 28460
Number of extensions: 92538
Number of successful extensions: 530
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 529
length of query: 198
length of database: 4,897,600
effective HSP length: 86
effective length of query: 112
effective length of database: 2,450,040
effective search space: 274404480
effective search space used: 274404480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146862.7