BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146862.5 + phase: 0 /pseudo
(624 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14681 similar to UP|Q9SLZ4 (Q9SLZ4) Retinoblastoma-related pro... 303 4e-83
TC12520 similar to UP|Q9SLZ4 (Q9SLZ4) Retinoblastoma-related pro... 261 2e-70
BP031900 56 2e-08
TC19351 similar to UP|H2A_CICAR (O65759) Histone H2A, partial (98%) 32 0.23
BP051688 30 1.5
TC12965 weakly similar to GB|AAP49518.1|31376383|BT008756 At3g62... 29 2.0
AV780303 28 5.7
AV766383 27 7.5
TC8393 weakly similar to GB|AAM61124.1|21536792|AY084557 prenyla... 27 7.5
TC7861 similar to UP|AAR86686 (AAR86686) Proline dehydrogenase ... 27 7.5
AV771990 27 9.8
TC16512 similar to UP|Q9FXL4 (Q9FXL4) Elicitor inducible chitina... 27 9.8
TC15624 27 9.8
>TC14681 similar to UP|Q9SLZ4 (Q9SLZ4) Retinoblastoma-related protein,
partial (22%)
Length = 911
Score = 303 bits (777), Expect = 4e-83
Identities = 147/174 (84%), Positives = 159/174 (90%)
Frame = +1
Query: 407 ILCCFYGVAKISQLNLTFREIIYNYRKQPQCKPQVFRSVFVDWSSARRNGGSRHRTGQEH 466
ILCCFYGVAKISQL++TFREII YRKQP CK QVFRSVFVDWSSARRNG S+ RTGQ+H
Sbjct: 1 ILCCFYGVAKISQLSVTFREIIQGYRKQPHCKHQVFRSVFVDWSSARRNGASKQRTGQDH 180
Query: 467 IDIISFYNEVFIPSVKPLLVELGPGGATVRSDQVPEANNKNDGHLVQNPGSPRISPFPSL 526
+DII+FYN++FIPSVKPLLVELGPGGAT++SDQ+ E NNKND Q PGSP+ISPFPSL
Sbjct: 181 VDIITFYNQIFIPSVKPLLVELGPGGATMKSDQMHEVNNKND---AQCPGSPKISPFPSL 351
Query: 527 PDMSPKKVSAAHNVYVSPLRSSKMDALISHSSKSYYACXGESTHAYQSPXKDLT 580
PDMSPKKVSAAHNVYVSPLRSSKMDALISHSSKSYYAC GESTHAYQSP KDLT
Sbjct: 352 PDMSPKKVSAAHNVYVSPLRSSKMDALISHSSKSYYACVGESTHAYQSPSKDLT 513
>TC12520 similar to UP|Q9SLZ4 (Q9SLZ4) Retinoblastoma-related protein,
partial (31%)
Length = 962
Score = 261 bits (668), Expect = 2e-70
Identities = 143/197 (72%), Positives = 157/197 (79%), Gaps = 1/197 (0%)
Frame = +1
Query: 120 S**SPWRPMCNWKPAKC*SHGQYMGRTTKIRSHEVIL*VVGNNVPSRSSNTWQQ-FNFTA 178
S**S W MCNWKPAKC HGQYMG T KIRS EV+L* +G+NV SRS NT FNF++
Sbjct: 7 S**SSW*TMCNWKPAKCKYHGQYMGGTAKIRSTEVLL*GIGSNVQSRSPNTTCN*FNFSS 186
Query: 179 H**EVP*VYACMFCRISPGYSQDCDNVVSCCTGEDWNNSF*S*QGDRKFH*I*RISPKRI 238
+**EV *VYACMFCRI PG SQDC+N V C TGEDW+ SF*S QGD KFH* *RI+PKRI
Sbjct: 187 N**EVS*VYACMFCRIGPGNSQDCNNAVPCSTGEDWDYSF*SEQGD*KFH*T*RIAPKRI 366
Query: 239 EATS*FIGGTAFGELGMGKRFFDV*FISSR*TCPFCRNQSAWYVSRTNAIIR*NCNAN*L 298
EATS IG T FGE GM K FF+V*F S *TCPFCRNQS+W VS TNAII *+CNA+*L
Sbjct: 367 EATSELIGRTTFGEHGMAKGFFNV*FFGSC*TCPFCRNQSSWNVS*TNAIIG*DCNAD*L 546
Query: 299 LLWRTASCAFLA*A*DF 315
LL R +SCAFLA*A*DF
Sbjct: 547 LLRRASSCAFLA*A*DF 597
Score = 145 bits (367), Expect = 1e-35
Identities = 72/79 (91%), Positives = 75/79 (94%)
Frame = +2
Query: 320 SKLLPPPLQSAFASPTKPNPGGGGETCAETGISVFFSKIVKLGAVRISGMVERLQLSQQI 379
SKL PPPLQSAFASPTKPNPGGGGETCAETGI++FF KI+KLGAVRISGMVERLQLSQQI
Sbjct: 725 SKLPPPPLQSAFASPTKPNPGGGGETCAETGINIFFGKIIKLGAVRISGMVERLQLSQQI 904
Query: 380 RENVYSLFQRILNQWTSLF 398
RENVY LFQRILNQ TSLF
Sbjct: 905 RENVYCLFQRILNQRTSLF 961
>BP031900
Length = 443
Score = 55.8 bits (133), Expect = 2e-08
Identities = 26/29 (89%), Positives = 26/29 (89%)
Frame = -3
Query: 552 ALISHSSKSYYACXGESTHAYQSPXKDLT 580
ALISHSSKSYYAC GESTHAYQS KDLT
Sbjct: 441 ALISHSSKSYYACFGESTHAYQSXSKDLT 355
>TC19351 similar to UP|H2A_CICAR (O65759) Histone H2A, partial (98%)
Length = 626
Score = 32.3 bits (72), Expect = 0.23
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +3
Query: 317 CLKSKLLPPPLQSAFASPTKPNP 339
CL KLLPPP ++ A+P PNP
Sbjct: 42 CLPQKLLPPPRRAEEANPKPPNP 110
>BP051688
Length = 497
Score = 29.6 bits (65), Expect = 1.5
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = +2
Query: 372 RLQLSQQIRENVYSLFQRILNQWTSLFFNRH 402
RL LS + R+N+Y + R +N W + RH
Sbjct: 302 RLLLSSKTRQNLYIMLLRCVNSWNNWLKRRH 394
>TC12965 weakly similar to GB|AAP49518.1|31376383|BT008756 At3g62130
{Arabidopsis thaliana;}, partial (18%)
Length = 471
Score = 29.3 bits (64), Expect = 2.0
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +1
Query: 504 NNKNDGHLVQNPGSPRISPFPSLPDMSPKKVSA 536
NN N+G P P++SP S P ++P ++ +
Sbjct: 148 NNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQS 246
>AV780303
Length = 562
Score = 27.7 bits (60), Expect = 5.7
Identities = 17/65 (26%), Positives = 32/65 (49%)
Frame = +3
Query: 487 ELGPGGATVRSDQVPEANNKNDGHLVQNPGSPRISPFPSLPDMSPKKVSAAHNVYVSPLR 546
E GPGGA V ++ + N ++ ++ PG I + P+ P++ Y PL+
Sbjct: 282 ESGPGGAGVILCEIEKPNAISN---LEMPGDLGIKKHAAEPEADPERDQRTVFAYQMPLK 452
Query: 547 SSKMD 551
+++ D
Sbjct: 453 ATERD 467
>AV766383
Length = 474
Score = 27.3 bits (59), Expect = 7.5
Identities = 13/32 (40%), Positives = 15/32 (46%)
Frame = +1
Query: 319 KSKLLPPPLQSAFASPTKPNPGGGGETCAETG 350
++ LLPPPL A AS GG G G
Sbjct: 268 ENSLLPPPLSPAAASSPTATTGGAGADAGGAG 363
>TC8393 weakly similar to GB|AAM61124.1|21536792|AY084557 prenylated Rab
receptor 2 {Arabidopsis thaliana;}, partial (78%)
Length = 1012
Score = 27.3 bits (59), Expect = 7.5
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = +1
Query: 323 LPPPLQSAFASPTKPNPGGGGETCAETGISV 353
LPPP SA +S T PNP A G S+
Sbjct: 139 LPPPPPSAPSSTTSPNPSATASLSAVPGRSL 231
>TC7861 similar to UP|AAR86686 (AAR86686) Proline dehydrogenase , partial
(40%)
Length = 943
Score = 27.3 bits (59), Expect = 7.5
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +1
Query: 517 SPRISPFPSLPDMSPKKVSAAHNVYVSP 544
SP +SP P L D +PK S+A +SP
Sbjct: 250 SPTLSPPPCLIDAAPKPPSSAATTLLSP 333
>AV771990
Length = 466
Score = 26.9 bits (58), Expect = 9.8
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = -3
Query: 195 SPGYSQDCDNVVSCCTG 211
SP S++CD +SCC G
Sbjct: 335 SPSSSRECDGSISCCWG 285
>TC16512 similar to UP|Q9FXL4 (Q9FXL4) Elicitor inducible chitinase
Nt-SubE76, partial (48%)
Length = 857
Score = 26.9 bits (58), Expect = 9.8
Identities = 16/42 (38%), Positives = 21/42 (49%), Gaps = 1/42 (2%)
Frame = +3
Query: 324 PPPLQSAFA-SPTKPNPGGGGETCAETGISVFFSKIVKLGAV 364
PPPL+S + +PT P+PG S S VKLG +
Sbjct: 93 PPPLRSPLSETPTPPHPGRSDPNWYSPFSSPCSSSSVKLGPI 218
>TC15624
Length = 546
Score = 26.9 bits (58), Expect = 9.8
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = -2
Query: 392 NQWTSLFFNRHIDQIILC 409
N+WT+LF+N H+ + LC
Sbjct: 434 NKWTALFYN*HVSKNNLC 381
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.342 0.148 0.505
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,536,954
Number of Sequences: 28460
Number of extensions: 150681
Number of successful extensions: 1244
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1242
length of query: 624
length of database: 4,897,600
effective HSP length: 96
effective length of query: 528
effective length of database: 2,165,440
effective search space: 1143352320
effective search space used: 1143352320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146862.5