BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146856.2 + phase: 0
(88 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%) 35 0.002
NP459451 NDX3 protein [Lotus japonicus] 30 0.057
BP063723 29 0.17
TC18302 similar to UP|Q8L6T7 (Q8L6T7) Ring-H2 zinc finger protei... 27 0.48
AV425984 26 1.1
BP073590 26 1.4
TC7996 homologue to UP|TBA_PRUDU (P33629) Tubulin alpha chain, c... 25 2.4
AV770385 24 4.1
BP065828 24 5.4
TC17317 homologue to UP|Q84VY4 (Q84VY4) At5g42860, partial (20%) 24 5.4
TC13221 similar to UP|PPAN_ARATH (Q9ASU7) Peter Pan-like protein... 23 7.0
TC17919 similar to UP|KPYA_RICCO (Q43117) Pyruvate kinase isozym... 23 7.0
TC13615 23 7.0
TC17116 weakly similar to UP|THM3_ARATH (Q9SEU7) Thioredoxin M-t... 23 9.1
AV415951 23 9.1
TC17943 23 9.1
BP042094 23 9.1
AV409767 23 9.1
>TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%)
Length = 519
Score = 35.0 bits (79), Expect = 0.002
Identities = 15/24 (62%), Positives = 17/24 (70%)
Frame = +2
Query: 26 VQWKGLSPEEATWEDEDEMRTSWP 49
V W+G S EEATWE ED MR +P
Sbjct: 317 VLWRGPSGEEATWEAEDIMREKYP 388
>NP459451 NDX3 protein [Lotus japonicus]
Length = 665
Score = 30.4 bits (67), Expect = 0.057
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = +3
Query: 34 EEATWEDEDEMRTSWPSPILEDKV 57
+EATWED +++ +PS LEDKV
Sbjct: 492 DEATWEDNITIKSQFPSFNLEDKV 563
>BP063723
Length = 479
Score = 28.9 bits (63), Expect = 0.17
Identities = 14/46 (30%), Positives = 25/46 (53%)
Frame = -3
Query: 26 VQWKGLSPEEATWEDEDEMRTSWPSPILEDKVVEGDGNDTYSLDSK 71
++WK L E +WED ++ +P+ LED++ G D + S+
Sbjct: 477 IRWKDLPTFEDSWEDFCKLLDPFPNHQLEDQLNLQGGRDVANPSSR 340
>TC18302 similar to UP|Q8L6T7 (Q8L6T7) Ring-H2 zinc finger protein, partial
(6%)
Length = 304
Score = 27.3 bits (59), Expect = 0.48
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +1
Query: 31 LSPEEATWEDEDEMRTSWPSPI 52
L+PE ATW T W SPI
Sbjct: 154 LNPETATWLHRQSQMTHWVSPI 219
>AV425984
Length = 351
Score = 26.2 bits (56), Expect = 1.1
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +1
Query: 21 KTQMLVQWKGLSPEEATWE 39
KT +L+QWK L E++WE
Sbjct: 7 KT*VLIQWKHLPISESSWE 63
>BP073590
Length = 381
Score = 25.8 bits (55), Expect = 1.4
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -1
Query: 11 TRSLRTPQGIKTQMLVQW 28
T+S+ TPQG + +VQW
Sbjct: 372 TKSIXTPQGYNSHCVVQW 319
>TC7996 homologue to UP|TBA_PRUDU (P33629) Tubulin alpha chain, complete
Length = 1907
Score = 25.0 bits (53), Expect = 2.4
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -2
Query: 64 DTYSLDSKHATEPGIRNE 81
D +S+ KH +PGIRN+
Sbjct: 1051 DYWSIRGKHKMDPGIRNQ 998
>AV770385
Length = 635
Score = 24.3 bits (51), Expect = 4.1
Identities = 12/39 (30%), Positives = 17/39 (42%)
Frame = -3
Query: 38 WEDEDEMRTSWPSPILEDKVVEGDGNDTYSLDSKHATEP 76
W+ + T WP ++E +EGD D H T P
Sbjct: 456 WKIYQHIPTRWPRTLVEKACMEGD-------DPIHGTMP 361
>BP065828
Length = 446
Score = 23.9 bits (50), Expect = 5.4
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 16 TPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWPSPI 52
T +GIKT M + K + TW + + R PSP+
Sbjct: 173 TGRGIKTPMPARNKQSAGRRKTWRELVKHRGFSPSPL 283
>TC17317 homologue to UP|Q84VY4 (Q84VY4) At5g42860, partial (20%)
Length = 566
Score = 23.9 bits (50), Expect = 5.4
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = -1
Query: 22 TQMLVQWKGLSPEEATWEDEDE 43
T++LV W +PE+ + E E+E
Sbjct: 554 TKILVSWPASTPEDESEEREEE 489
>TC13221 similar to UP|PPAN_ARATH (Q9ASU7) Peter Pan-like protein, partial
(15%)
Length = 521
Score = 23.5 bits (49), Expect = 7.0
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +3
Query: 30 GLSPEEATWEDEDEM 44
G PEEA+ ED+DE+
Sbjct: 249 GEDPEEASLEDDDEL 293
>TC17919 similar to UP|KPYA_RICCO (Q43117) Pyruvate kinase isozyme A,
chloroplast precursor , partial (11%)
Length = 566
Score = 23.5 bits (49), Expect = 7.0
Identities = 15/32 (46%), Positives = 17/32 (52%), Gaps = 3/32 (9%)
Frame = -2
Query: 31 LSPEEATWEDEDE---MRTSWPSPILEDKVVE 59
LSPE T EDE+E MR W + K VE
Sbjct: 331 LSPEMRTSEDEEEVERMRGRWWRVVRGKKSVE 236
>TC13615
Length = 350
Score = 23.5 bits (49), Expect = 7.0
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +1
Query: 37 TWEDEDEMRTSWPSPILEDKVVE 59
TW EM + W S +L D+++E
Sbjct: 235 TW*FNQEMFSYWGSVLLHDRLIE 303
>TC17116 weakly similar to UP|THM3_ARATH (Q9SEU7) Thioredoxin M-type 3,
chloroplast precursor (TRX-M3), partial (55%)
Length = 798
Score = 23.1 bits (48), Expect = 9.1
Identities = 9/21 (42%), Positives = 12/21 (56%)
Frame = -3
Query: 27 QWKGLSPEEATWEDEDEMRTS 47
+WKG E WE E+E + S
Sbjct: 79 KWKGNEREGLRWEREEERKGS 17
>AV415951
Length = 366
Score = 23.1 bits (48), Expect = 9.1
Identities = 14/46 (30%), Positives = 21/46 (45%)
Frame = +2
Query: 12 RSLRTPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWPSPILEDKV 57
+ L TP+ +L + EE E+E++ W ILED V
Sbjct: 215 KPLITPRKDPAGVLEEHGYAYEEEDDEEEEEDKWVDWEDQILEDTV 352
>TC17943
Length = 442
Score = 23.1 bits (48), Expect = 9.1
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +3
Query: 37 TWEDEDEMRTSWPSPILEDKVVE 59
TW EM + W S +L D+++E
Sbjct: 363 TW*FNKEMFSYWGSVLLHDRLIE 431
>BP042094
Length = 409
Score = 23.1 bits (48), Expect = 9.1
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -1
Query: 37 TWEDEDEMRTSWPSPILEDK 56
+W DE ++ +PS LEDK
Sbjct: 406 SWVDEPALKCQFPSFSLEDK 347
>AV409767
Length = 411
Score = 23.1 bits (48), Expect = 9.1
Identities = 7/11 (63%), Positives = 10/11 (90%)
Frame = +3
Query: 28 WKGLSPEEATW 38
W+GLSP+E T+
Sbjct: 186 WRGLSPDEGTY 218
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.307 0.128 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,530,388
Number of Sequences: 28460
Number of extensions: 17946
Number of successful extensions: 79
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 79
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 79
length of query: 88
length of database: 4,897,600
effective HSP length: 64
effective length of query: 24
effective length of database: 3,076,160
effective search space: 73827840
effective search space used: 73827840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 48 (23.1 bits)
Medicago: description of AC146856.2