BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146856.10 + phase: 0
(1260 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP566646 hypothetical protein [Lotus japonicus] 31 1.1
TC16870 31 1.4
TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-... 31 1.4
TC11336 similar to UP|Q8S9A5 (Q8S9A5) Glucosyltransferase like p... 30 3.1
TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, ... 30 3.1
TC12847 similar to GB|AAM66074.1|21595125|AY088542 endosperm-spe... 30 3.1
AV425247 29 4.0
TC12483 weakly similar to UP|Q40552 (Q40552) Pistil extensin lik... 29 4.0
TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich... 29 4.0
AV767945 29 5.3
AV765282 29 5.3
AV425317 28 6.9
TC13698 similar to GB|AAO63291.1|28950735|BT005227 At4g20000 {Ar... 28 6.9
TC12762 28 6.9
TC15366 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Ar... 28 6.9
TC18736 similar to UP|Q40485 (Q40485) C1C-Nt1 protein, partial (... 28 6.9
TC9927 similar to UP|Q8L7U3 (Q8L7U3) At1g14620/T5E21_15, partial... 28 6.9
CB828699 28 6.9
TC11657 similar to UP|O04292 (O04292) HD-Zip protein (Homeodomai... 28 9.0
TC11199 28 9.0
>NP566646 hypothetical protein [Lotus japonicus]
Length = 996
Score = 31.2 bits (69), Expect = 1.1
Identities = 18/46 (39%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = -2
Query: 49 LPPSLYESITTTIQSFLNLLELNNSENPQT-PPTATLRRSSRKNTT 93
LPPS+++ T I F + ++S +P + PPTA+ R S R +TT
Sbjct: 518 LPPSIFDFSTWLIIGFFSSCLCSSSSSPTSPPPTASTR*SVRWSTT 381
>TC16870
Length = 492
Score = 30.8 bits (68), Expect = 1.4
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = -1
Query: 720 RCHIKFGADMPSHSVTATKRKKSKSSGKFSIKSKIINRK 758
R H+ G D +S T T+RKK K +G + SKI R+
Sbjct: 120 RTHLTMGGDANQNSTT-TRRKKQKKNGHLNPTSKISRRR 7
>TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-like
protein, partial (83%)
Length = 1784
Score = 30.8 bits (68), Expect = 1.4
Identities = 28/89 (31%), Positives = 37/89 (41%), Gaps = 6/89 (6%)
Frame = +2
Query: 5 LCSSLNSSPQEFISLATTLTLKSSKSSLKTL------IHSIKPSSNLISSLPPSLYESIT 58
LC L+SS S + + +LK + SS T H P S S PPS + T
Sbjct: 68 LCLLLHSSSSFLCSPSFSASLKPNPSSASTTAKSPTTFHRQSPPSTS-SDPPPSEKSAST 244
Query: 59 TTIQSFLNLLELNNSENPQTPPTATLRRS 87
I + SE+ PPTAT + S
Sbjct: 245 APIPRSSRPSPIPESESSSAPPTATFQIS 331
>TC11336 similar to UP|Q8S9A5 (Q8S9A5) Glucosyltransferase like protein
(Fragment), partial (27%)
Length = 511
Score = 29.6 bits (65), Expect = 3.1
Identities = 27/102 (26%), Positives = 47/102 (45%), Gaps = 5/102 (4%)
Frame = +1
Query: 4 KLCSSLNSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLY----ESITT 59
K SSL+ Q F+ + ++ + + +HS+ ++ L S PP+L S +
Sbjct: 205 KPSSSLSLHTQTFLQVQRIFVRSATLETTPSSMHSLNSNNPLSSGSPPTLTPLWPSSPIS 384
Query: 60 TIQSFLNLLELNNSENPQTPPTATLRRSSRKNTTA-TTEPSS 100
+ N L NS +P++ TA+ R S +T T+P S
Sbjct: 385 SSDGLTN--SLPNSASPESHFTASARSSPPCSTAVFETQPHS 504
>TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, partial
(68%)
Length = 1097
Score = 29.6 bits (65), Expect = 3.1
Identities = 31/109 (28%), Positives = 45/109 (40%), Gaps = 13/109 (11%)
Frame = +1
Query: 7 SSLNSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSFLN 66
++ NSSP E S T S + L P S+ S+ PPS S T ++ +
Sbjct: 205 AATNSSPPESRS---TTPPSSEPTCLLAPASPPTPKSSHSSTPPPSRSSSTTPKSKNATS 375
Query: 67 LLELN-------------NSENPQTPPTATLRRSSRKNTTATTEPSSQS 102
L + S+ P PPT + + SS K++ T PSS S
Sbjct: 376 LTAPSCPPPASPAATAPAPSKTPPNPPTNSSKPSSCKSSLPNTTPSSLS 522
>TC12847 similar to GB|AAM66074.1|21595125|AY088542 endosperm-specific
protein-like protein {Arabidopsis thaliana;} , partial
(11%)
Length = 245
Score = 29.6 bits (65), Expect = 3.1
Identities = 22/61 (36%), Positives = 31/61 (50%)
Frame = +1
Query: 40 KPSSNLISSLPPSLYESITTTIQSFLNLLELNNSENPQTPPTATLRRSSRKNTTATTEPS 99
+PS +L L P + +TT + S + L + P +PPT + R S TTA T PS
Sbjct: 4 QPSLSLPPPLSPISPKWVTTPLSS--SSLLSPSPPPPPSPPTTSPRFSPPTPTTAPTTPS 177
Query: 100 S 100
S
Sbjct: 178 S 180
>AV425247
Length = 417
Score = 29.3 bits (64), Expect = 4.0
Identities = 23/72 (31%), Positives = 31/72 (42%)
Frame = +2
Query: 48 SLPPSLYESITTTIQSFLNLLELNNSENPQTPPTATLRRSSRKNTTATTEPSSQSDEKHK 107
SLPPS +I SF + S +P TPP TL SS P++ H
Sbjct: 194 SLPPSSIHTI-----SFPFPFPITPSPSPNTPPPLTLCPSSPSPLALIFPPTASPSHPHL 358
Query: 108 LLEKLQILAHIL 119
+ K Q +H+L
Sbjct: 359 ICPK-QPTSHLL 391
>TC12483 weakly similar to UP|Q40552 (Q40552) Pistil extensin like protein
(Fragment), partial (6%)
Length = 534
Score = 29.3 bits (64), Expect = 4.0
Identities = 29/87 (33%), Positives = 41/87 (46%), Gaps = 4/87 (4%)
Frame = +3
Query: 16 FISLATTLTLKSSKSSL-KTLIHSIK---PSSNLISSLPPSLYESITTTIQSFLNLLELN 71
F SL L L SS S L + L+ S+ P +N SLP L++ I L L
Sbjct: 27 FSSLLPHLKLISSSSDLPQLLLRSLSLTPPPNNHFPSLP--LFKEI-------LKPFPLP 179
Query: 72 NSENPQTPPTATLRRSSRKNTTATTEP 98
+ NP+T P+ + + TTAT +P
Sbjct: 180 SP*NPKTLPSPFTKSGHQTTTTATKQP 260
>TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich protein 1
(NDPP-1). {Mus musculus;}, partial (8%)
Length = 1134
Score = 29.3 bits (64), Expect = 4.0
Identities = 25/82 (30%), Positives = 35/82 (42%)
Frame = +3
Query: 19 LATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSFLNLLELNNSENPQT 78
L TT + SS SS + S PS +L PP L + S L E +++ P T
Sbjct: 114 LTTTASASSSSSSSPS---SS*PSPSLPPPSPPPLPPPQAPPLPSHLPSPEPSSTMPPST 284
Query: 79 PPTATLRRSSRKNTTATTEPSS 100
PP + +T + PSS
Sbjct: 285 PPPQPTKPCPLPKSTPSQPPSS 350
>AV767945
Length = 290
Score = 28.9 bits (63), Expect = 5.3
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +3
Query: 425 PSPIITKMLTKVISDMSHDVCNEVRLSTLS 454
P P+ TK++T +I HD C + +ST++
Sbjct: 93 PCPVKTKVITMLIRKQ*HDSCTPILISTVT 182
>AV765282
Length = 530
Score = 28.9 bits (63), Expect = 5.3
Identities = 24/92 (26%), Positives = 37/92 (40%)
Frame = +1
Query: 22 TLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSFLNLLELNNSENPQTPPT 81
+L K K LKTL +P+ N P LY ++LLE N P
Sbjct: 76 SLWRKPPKQPLKTLTTRKRPNQN-----PSDLYNRD-------MSLLENKNLMQPHKSKD 219
Query: 82 ATLRRSSRKNTTATTEPSSQSDEKHKLLEKLQ 113
T + R ++ +D+KH +LE ++
Sbjct: 220 LTAKTYKRHRAVNRNLTNTNNDKKH*ILEMIR 315
>AV425317
Length = 398
Score = 28.5 bits (62), Expect = 6.9
Identities = 22/59 (37%), Positives = 29/59 (48%)
Frame = +3
Query: 40 KPSSNLISSLPPSLYESITTTIQSFLNLLELNNSENPQTPPTATLRRSSRKNTTATTEP 98
K SS + +L P L + TT+ FL L S NP+T P +SS TTA +P
Sbjct: 45 KSSSMELETLAPLLL--LFTTVIWFLATTTLLKSSNPKTTP-----QSSTSTTTAIPKP 200
>TC13698 similar to GB|AAO63291.1|28950735|BT005227 At4g20000 {Arabidopsis
thaliana;}, partial (10%)
Length = 419
Score = 28.5 bits (62), Expect = 6.9
Identities = 15/32 (46%), Positives = 18/32 (55%)
Frame = +2
Query: 903 KNRSSKKRKRTGKNQNSTKRRRLSLTNAGCPS 934
++RSSKK T N N+T R L GCPS
Sbjct: 242 RSRSSKKTPTTLLNANTTNFRALVQQFTGCPS 337
>TC12762
Length = 865
Score = 28.5 bits (62), Expect = 6.9
Identities = 16/45 (35%), Positives = 24/45 (52%)
Frame = +1
Query: 65 LNLLELNNSENPQTPPTATLRRSSRKNTTATTEPSSQSDEKHKLL 109
L+LL ++SE PQ+PP SR + A + PS + +H L
Sbjct: 436 LHLLTFSSSEPPQSPP-------SRATSIADSRPSKSAS*RHTTL 549
>TC15366 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Arabidopsis
thaliana;}, partial (23%)
Length = 568
Score = 28.5 bits (62), Expect = 6.9
Identities = 18/46 (39%), Positives = 23/46 (49%), Gaps = 1/46 (2%)
Frame = +2
Query: 41 PSSNLISSL-PPSLYESITTTIQSFLNLLELNNSENPQTPPTATLR 85
P SNL S L PP L++S TT + L S+NP +LR
Sbjct: 194 PISNLTSFLSPPELHKSFTTATDNCFRFLHSLASQNPFLNKILSLR 331
>TC18736 similar to UP|Q40485 (Q40485) C1C-Nt1 protein, partial (15%)
Length = 815
Score = 28.5 bits (62), Expect = 6.9
Identities = 22/96 (22%), Positives = 38/96 (38%), Gaps = 6/96 (6%)
Frame = +1
Query: 940 HNEPQQVLCKVKMITAVLKFMADATA-MCFAPHNNG-----SFLNNTSKCIQHILSSLNQ 993
++ P V+ + + A + F MC P + G L +H+L
Sbjct: 223 NSSPHTVVETMSLAKAAIIFRQQGLRHMCVVPKSQGRPPVVGILTRHDFMPEHVLGLYPD 402
Query: 994 LYHHKIEFEEEDKKNTIFCLKSSFSYAAKILNVILT 1029
+ HHK K+N+ F ++ S+AA V+ T
Sbjct: 403 IKHHKWH*GISMKRNSFFSIEVLHSFAAAGFKVLYT 510
>TC9927 similar to UP|Q8L7U3 (Q8L7U3) At1g14620/T5E21_15, partial (34%)
Length = 602
Score = 28.5 bits (62), Expect = 6.9
Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = -2
Query: 108 LLEKLQILAHILF-LCVSHPRKVFDFSDLLPGVQALHDNLIVLEADSILSSG 158
L++KL +L IL L +SHP K+ FSD V+ + N + LE + + G
Sbjct: 247 LVKKLSLLRKILQQLILSHPNKILTFSD----VKLVFSNYL*LEEEILKRCG 104
>CB828699
Length = 531
Score = 28.5 bits (62), Expect = 6.9
Identities = 31/137 (22%), Positives = 49/137 (35%), Gaps = 19/137 (13%)
Frame = +3
Query: 10 NSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPS---LYESITTTIQSFLN 66
N L + SS++ +L +S +S+ ++S P L + +T+ N
Sbjct: 30 NKQTLSLYCLTMAVAFPSSRTETSSLSYSSAFASSTVASFSPRASPLMSTNSTSAAVIAN 209
Query: 67 LLELNNSENPQTPPTATL---------------RRSSRKNTTATTEPSSQSDEKHKLLEK 111
+ ++ P TPP+ RRS R N A SS +EK
Sbjct: 210 VRLRDHRSLPPTPPSPPSSVKLSVERRPPVSPGRRSMRNNNNAAVVSSSVKEEKRTPACM 389
Query: 112 LQILAHI-LFLCVSHPR 127
+H F C H R
Sbjct: 390 CSPTSHAGSFRCAYHKR 440
>TC11657 similar to UP|O04292 (O04292) HD-Zip protein (Homeodomain-leucine
zipper protein), partial (9%)
Length = 564
Score = 28.1 bits (61), Expect = 9.0
Identities = 14/30 (46%), Positives = 18/30 (59%), Gaps = 4/30 (13%)
Frame = +2
Query: 652 FNIFSTVCPDNVAGLL----EECMSVVTNC 677
FN+F T CPD V +L EC+S +NC
Sbjct: 425 FNLFLTSCPDPVPLILLITGSECLSGDSNC 514
>TC11199
Length = 743
Score = 28.1 bits (61), Expect = 9.0
Identities = 22/100 (22%), Positives = 45/100 (45%), Gaps = 4/100 (4%)
Frame = +1
Query: 10 NSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSFLNLLE 69
+SSP + + T + SS + HS S+ ++ P S T ++ +
Sbjct: 118 SSSPPPYATTRT*DPSSARPSSPASQSHSTTTSATSLAPKSPRSKRSARRTTRTSSSPST 297
Query: 70 LNN----SENPQTPPTATLRRSSRKNTTATTEPSSQSDEK 105
+++ + NP +PP+ T SS + ++ PS+ S ++
Sbjct: 298 ISDPSSPTSNPSSPPSLTPTPSSSPSLALSSPPSTPSSKR 417
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.134 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,637,823
Number of Sequences: 28460
Number of extensions: 276127
Number of successful extensions: 1609
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1588
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1608
length of query: 1260
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1159
effective length of database: 2,023,140
effective search space: 2344819260
effective search space used: 2344819260
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146856.10