BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146819.5 + phase: 0 /partial
(599 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV406876 34 0.078
TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partia... 33 0.17
TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-... 33 0.17
TC17474 similar to UP|Q8VXT1 (Q8VXT1) Protein kinase 2, partial ... 30 1.1
TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%) 29 1.9
AW163946 28 3.3
TC13530 similar to UP|C5P1_ARATH (Q8H0V1) CDK5RAP1-like protein,... 28 3.3
TC15308 similar to UP|Q41725 (Q41725) TED3, partial (16%) 28 3.3
TC13077 28 3.3
BP037162 28 4.3
TC14555 similar to UP|Q43558 (Q43558) Proline rich protein precu... 27 7.3
NP817340 galactomannan galactosyltransferase [Lotus japonicus] 27 7.3
AV764700 27 7.3
AV779582 27 9.5
TC15125 weakly similar to UP|Q84P20 (Q84P20) Acyl-activating enz... 27 9.5
TC8002 similar to GB|AAA28385.1|156990|DROBCD segmentation prote... 27 9.5
>AV406876
Length = 234
Score = 33.9 bits (76), Expect = 0.078
Identities = 20/57 (35%), Positives = 30/57 (52%)
Frame = -1
Query: 287 SSLGSGLQNQASRSTATHPQSVNLIPQNQASQPITINAMPLQNYRLQNQISRPNNAP 343
SS S Q+ A+++ + P + IPQ Q SQ + N QN+ QN +S N+ P
Sbjct: 204 SSFHS*QQHPATKTCHSEPSTT*PIPQQQTSQHLPQNHHTHQNHHPQNPLSPQNSPP 34
>TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partial (27%)
Length = 997
Score = 32.7 bits (73), Expect = 0.17
Identities = 55/245 (22%), Positives = 98/245 (39%), Gaps = 23/245 (9%)
Frame = +3
Query: 162 LPIAMNARSSLGSGLQLQVSRSAETHPLSVNLMPQNQASRQITMNAYSSLGSGLQNQASR 221
LP ++ S L L +S PL ++L + Q S + + S +G+ + Q +R
Sbjct: 3 LPSPACSKKEKVSSLSLSLSTPTCYSPL-LSLSFKTQISFTLFL---SPVGNSGERQTTR 170
Query: 222 STETHPHSVNLMPQNQVSRQIT-MNACSSLGSGLQNQASRSTATHPHS------------ 268
E+ S+ ++P S M C + S L+ R + HPH
Sbjct: 171 KRESKNLSIPVVPLRPKSPPPPYMPPCFTSLSLLRPSRRRHQSHHPHHNPTIQKKYYL*T 350
Query: 269 ------VNLMPQ----NQVSRQITMNACSSLGSGLQNQASRSTATHPQSVNLIPQNQASQ 318
+N + Q + S++ N C S L Q+S +T P + P + +
Sbjct: 351 SWWGD*LNSIDQANKKQRQSQEFRFNRCCC--SCLHYQSSIQLSTSPP*PSSPPSSSSLF 524
Query: 319 PITINAMPLQNYRLQNQISRPNNAPGCSTASNFTIPNGANNGICQESGPTLARNRYPSNT 378
++++ +S PN +P S AS + P+ +++ SGP+ + YPS+
Sbjct: 525 SPSVSSSTF--------VSNPNPSP-ISKASTASGPSVSSSSSSSSSGPSPNSSGYPSSA 677
Query: 379 AARMP 383
A P
Sbjct: 678 AGTSP 692
>TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-like
protein, partial (97%)
Length = 2230
Score = 32.7 bits (73), Expect = 0.17
Identities = 33/147 (22%), Positives = 61/147 (41%), Gaps = 3/147 (2%)
Frame = +1
Query: 225 THPHSVNLMPQNQVSRQITMNAC---SSLGSGLQNQASRSTATHPHSVNLMPQNQVSRQI 281
+H HS+N + ++ +QI + C S ++SRS+ + N +
Sbjct: 91 SHSHSINNRRKRKIKKQIWVRKCLHWRRNSSPQPPESSRSSELTA*TANSTAASPPPAPC 270
Query: 282 TMNACSSLGSGLQNQASRSTATHPQSVNLIPQNQASQPITINAMPLQNYRLQNQISRPNN 341
+ SS S L AS+++ T P + P S + +A P +R ++ + P++
Sbjct: 271 SCGPSSSSPSPLPTSASKASLT-PAAATSPPPGAGSSGRSKSAPP---HRSTDRAACPSS 438
Query: 342 APGCSTASNFTIPNGANNGICQESGPT 368
+P +S T P+ SG T
Sbjct: 439 SPAPPASSAHTFPSP*KGEATASSGST 519
Score = 28.1 bits (61), Expect = 4.3
Identities = 50/281 (17%), Positives = 96/281 (33%), Gaps = 10/281 (3%)
Frame = +1
Query: 247 CSSLGSGLQNQASRSTATHPHSVNLMPQNQVSRQITMNAC---SSLGSGLQNQASRSTAT 303
C S + ++ +H HS+N + ++ +QI + C S ++SRS+
Sbjct: 40 CFSFFISIFTNQTQPNPSHSHSINNRRKRKIKKQIWVRKCLHWRRNSSPQPPESSRSSEL 219
Query: 304 HPQSVNLIPQNQASQPITINAMPLQNYRLQNQISRPNNAPGCSTASNFTIPNGANNGICQ 363
+ N + P + L S+ + P +T+ P ++G +
Sbjct: 220 TA*TANSTAASPPPAPCSCGPSSSSPSPLPTSASKASLTPAAATSPP---PGAGSSGRSK 390
Query: 364 ESGPTLARNRYPSNTAARMPLGVRNHAIQKK-RGHGVGTGNPVTMNHVTAP--GAPGFSN 420
+ P + +R +++ P H +G + T T P +
Sbjct: 391 SAPPHRSTDRAACPSSSPAPPASSAHTFPSP*KGEATASSGSTTSTTTTTHR*KKPAKPS 570
Query: 421 HINTQYSDRSNAAAATGFSNSRNGYGQDGIRITNAINPLLTQPSHSL----AYETQPSYQ 476
T +S + FS S + + + + T+ P P L + PS
Sbjct: 571 SPPTAFSSWKATSTMPSFSPSSSTWWRSPMSCTSPRRPESATPWRILIPTFTATSPPSSP 750
Query: 477 SNSIQNLYGYNNFQGDESLSSYVARLNTNRYLNEHQIGSQN 517
S NL N L + ++T R+ + + IG N
Sbjct: 751 SWKPANLQTRNPLL--SGLLQAPSTVSTRRFRSRNPIGLTN 867
>TC17474 similar to UP|Q8VXT1 (Q8VXT1) Protein kinase 2, partial (30%)
Length = 560
Score = 30.0 bits (66), Expect = 1.1
Identities = 11/32 (34%), Positives = 16/32 (49%)
Frame = -2
Query: 7 PHSRHLCGNFPYSSTPHERHCGQCHCYVCDSP 38
PH + LC + P++R CHC+ C P
Sbjct: 313 PHMK*LCSHRAIFCCPNQREIWMCHCFSCR*P 218
>TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%)
Length = 1240
Score = 29.3 bits (64), Expect = 1.9
Identities = 42/144 (29%), Positives = 51/144 (35%), Gaps = 1/144 (0%)
Frame = +1
Query: 83 SQPNHMLPLETARLSPNSVLVNHTSRSTALLPLSANLIPQNQAYWNACSSLSSRLQNQVS 142
S P PL SP+S S STA SA P A S+ S R Q S
Sbjct: 181 SPPASTSPLTPPTTSPSS------STSTAAPSASA---PLTTTPTTATSTTSPRRQTPSS 333
Query: 143 R-STETHPHSVNLIPQNQAYLPIAMNARSSLGSGLQLQVSRSAETHPLSVNLMPQNQASR 201
ST P S P SGL +R + T P+ + P + ASR
Sbjct: 334 SPSTTASPRSTPS--------PSPTTTPGKPSSGLPASTTRGSRTTPIPILFSPSSTASR 489
Query: 202 QITMNAYSSLGSGLQNQASRSTET 225
+ A G + SRS ET
Sbjct: 490 RR*HRAQ----HGDARKCSRSVET 549
>AW163946
Length = 460
Score = 28.5 bits (62), Expect = 3.3
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 4/73 (5%)
Frame = -2
Query: 253 GLQNQASRSTATHPHSVNLMPQNQVSRQITMNACSSLGSGLQNQASRSTATHPQSVNLIP 312
GLQ QAS TH S + + + + + + SS+ S + + ATH L P
Sbjct: 303 GLQCQASSWACTHQRSSHQLACTLLPQLESYHNISSVNSSREVHSGP*HATHNLEEKLHP 124
Query: 313 Q----NQASQPIT 321
Q N+AS P T
Sbjct: 123 QSRPANRASHPAT 85
>TC13530 similar to UP|C5P1_ARATH (Q8H0V1) CDK5RAP1-like protein, partial
(11%)
Length = 627
Score = 28.5 bits (62), Expect = 3.3
Identities = 23/77 (29%), Positives = 34/77 (43%), Gaps = 13/77 (16%)
Frame = +2
Query: 217 NQASRSTETHPHSVNLMPQNQVSRQITMNACSSLGSGLQNQASRSTATH----------- 265
+QAS S H HS NL+P Q+ Q ++ S L A+ S + H
Sbjct: 137 SQASPSILFHHHSQNLLPPTQIQTQHFFSSTHIWLSRLHFTAAPSQSHHRLHNLPSFHQI 316
Query: 266 --PHSVNLMPQNQVSRQ 280
PHS L+ ++ SR+
Sbjct: 317 RGPHSPPLLHRSSHSRR 367
Score = 27.7 bits (60), Expect = 5.6
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +2
Query: 256 NQASRSTATHPHSVNLMPQNQVSRQITMNACSSLGSGLQNQASRSTATH 304
+QAS S H HS NL+P Q+ Q ++ S L A+ S + H
Sbjct: 137 SQASPSILFHHHSQNLLPPTQIQTQHFFSSTHIWLSRLHFTAAPSQSHH 283
>TC15308 similar to UP|Q41725 (Q41725) TED3, partial (16%)
Length = 1103
Score = 28.5 bits (62), Expect = 3.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -3
Query: 12 LCGNFPYSSTPHERHCGQCHCYVCDSPAPC 41
LC +F SS+ +C C CY C S PC
Sbjct: 609 LCHSFLPSSSHSHYYCHNC-CYCCCSKVPC 523
>TC13077
Length = 670
Score = 28.5 bits (62), Expect = 3.3
Identities = 27/119 (22%), Positives = 47/119 (38%), Gaps = 4/119 (3%)
Frame = +1
Query: 57 TDKSDIWK--TLRQDSLSASTKSGNVVNSQPNHMLPLETARLSPNSVLVNHTSRSTALLP 114
+ K+D W L+ + + G VNS P ++ + N +L + + +
Sbjct: 199 SSKADEWTGDMLQTGDIVEELRIGGSVNSLIRFKSPFKSGKSGVNKILQDAYKKKETSIC 378
Query: 115 LSANLIPQNQAYWNAC--SSLSSRLQNQVSRSTETHPHSVNLIPQNQAYLPIAMNARSS 171
+ P A W AC + SS +N V RS + V + + +A +RSS
Sbjct: 379 VRVRRGPDLFAEWQACIVPNESSGKKNYVLRSISDPNYVVGFLDRTEAECFQLQASRSS 555
>BP037162
Length = 518
Score = 28.1 bits (61), Expect = 4.3
Identities = 14/44 (31%), Positives = 19/44 (42%)
Frame = -2
Query: 24 ERHCGQCHCYVCDSPAPCLKWGDGLLNTDHCHATDKSDIWKTLR 67
+R+C C C+ C S A C + HCH W+ LR
Sbjct: 307 QRNCHCCCCWWCSSSASC---------SGHCHEWFHWRWWRKLR 203
>TC14555 similar to UP|Q43558 (Q43558) Proline rich protein precursor,
partial (43%)
Length = 1038
Score = 27.3 bits (59), Expect = 7.3
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +3
Query: 83 SQPNHMLPLETARL-SPNSVLVNHTSRSTALLPLSANLIPQN 123
S+ NH L+ L P+ L+NH S LLPL+ L+P N
Sbjct: 177 SEHNHHPTLQPPPLLHPHQPLLNHLLLSLLLLPLNLLLLPFN 302
>NP817340 galactomannan galactosyltransferase [Lotus japonicus]
Length = 1314
Score = 27.3 bits (59), Expect = 7.3
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -3
Query: 27 CGQCHCYVCDSPAPC 41
C C C+VCDS PC
Sbjct: 484 CRLCLCHVCDSSPPC 440
>AV764700
Length = 434
Score = 27.3 bits (59), Expect = 7.3
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = -2
Query: 512 QIGSQNENIINSGATVQDVFQQNPHGGIQNEGF 544
++G + E+I+ G T DVF Q+P G Q++ F
Sbjct: 400 RVGVKAESILYEGKTHTDVFLQDPMRGGQDDMF 302
>AV779582
Length = 345
Score = 26.9 bits (58), Expect = 9.5
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +2
Query: 426 YSDRSNAAAATGFSNSRNGYGQDGIRITNAINPLLTQPSHSLAYETQPSYQS 477
YSD N + T S+ G+G +G N+ NP + PS+ + + +Y+S
Sbjct: 50 YSDPINPSEPTRRSSGWGGFGSEG---ENS*NPTQSNPSNGNGFGS*LAYKS 196
>TC15125 weakly similar to UP|Q84P20 (Q84P20) Acyl-activating enzyme 12,
partial (48%)
Length = 1164
Score = 26.9 bits (58), Expect = 9.5
Identities = 12/42 (28%), Positives = 17/42 (39%)
Frame = -1
Query: 389 HAIQKKRGHGVGTGNPVTMNHVTAPGAPGFSNHINTQYSDRS 430
H RGH P+ M H P F+ H ++Y +S
Sbjct: 888 HISTASRGHTPWESPPIAMEHWQRPKVDRFTTHFPSKYEAQS 763
>TC8002 similar to GB|AAA28385.1|156990|DROBCD segmentation protein PRD 4
{Drosophila melanogaster;} , partial (50%)
Length = 640
Score = 26.9 bits (58), Expect = 9.5
Identities = 21/103 (20%), Positives = 42/103 (40%), Gaps = 1/103 (0%)
Frame = +1
Query: 227 PHSVNLMPQNQVSRQITMNACSSLGSGLQNQASRSTATHPHSV-NLMPQNQVSRQITMNA 285
PH+ + M Q + + + + + L + S+ T H L P+N + A
Sbjct: 127 PHNTHTM-QTMSTTMASFSPLTRFSATLSSSNSKPHLTQNHFFFTLRPKNLPLSPLKWKA 303
Query: 286 CSSLGSGLQNQASRSTATHPQSVNLIPQNQASQPITINAMPLQ 328
S G+G + A+ + + P+ +++ AS A L+
Sbjct: 304 SSDNGAGASDSAATAVESPPEKISVGTNGSASSAAAAAAEELK 432
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.125 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,395,043
Number of Sequences: 28460
Number of extensions: 179943
Number of successful extensions: 816
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 743
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 813
length of query: 599
length of database: 4,897,600
effective HSP length: 95
effective length of query: 504
effective length of database: 2,193,900
effective search space: 1105725600
effective search space used: 1105725600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146819.5