BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146819.1 + phase: 0
(221 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13455 weakly similar to UP|Q9LG41 (Q9LG41) Dimethylaniline mon... 109 4e-25
AV774936 99 6e-22
AV777386 80 4e-16
BP043294 79 8e-16
BP074117 63 5e-11
TC17820 58 1e-09
TC14075 homologue to UP|PRP2_MEDTR (Q40375) Repetitive proline-r... 29 0.73
BI418441 29 0.73
TC16202 similar to UP|Q9GFF4 (Q9GFF4) Photosystem II D2 protein ... 28 0.96
TC16033 UP|Q7X9C1 (Q7X9C1) NIN-like protein 1, partial (88%) 28 1.6
TC10951 weakly similar to UP|Q93Z11 (Q93Z11) AT5g37360/MNJ8_150,... 28 1.6
TC17127 similar to UP|Q8L751 (Q8L751) AT4g08180/T12G13_20, parti... 27 2.1
TC16506 homologue to UP|Q8VYH4 (Q8VYH4) AT5g15260/F8M21_150, par... 27 2.1
TC18122 similar to UP|Q8VZY4 (Q8VZY4) 1-aminocyclopropanecarboxy... 27 3.6
TC11148 homologue to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane tra... 27 3.6
TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transc... 26 4.7
CB827010 26 6.2
TC18257 weakly similar to UP|Q9LFY5 (Q9LFY5) T7N9.6, partial (46%) 25 8.1
TC8467 similar to UP|Q8L751 (Q8L751) AT4g08180/T12G13_20, partia... 25 8.1
>TC13455 weakly similar to UP|Q9LG41 (Q9LG41) Dimethylaniline
monooxygenase-like protein, partial (9%)
Length = 535
Score = 109 bits (272), Expect = 4e-25
Identities = 53/129 (41%), Positives = 78/129 (60%), Gaps = 1/129 (0%)
Frame = +1
Query: 68 YGIKRPSMGPLQLKNTVGKTPVLDIGALEKIRSGDINVVPGIKRINKNGEVELVNGEKLD 127
YGI RP+ GP +K GK PV+D G + KI+SGD+ V+P ++ V L NGE
Sbjct: 4 YGIARPNEGPFCMKVKYGKYPVIDTGTIHKIKSGDLKVLPSEIEYVRDKNVLLKNGELHP 183
Query: 128 IDAVVLATGYRSNVPSWLQEGEF-FSKNGYPKMPFPHGWKGNSGLYAVGFTKRGLSGASS 186
D+++ TG++ + WL+ ++ + +G PK +P WKG GLY VG ++RGL GA++
Sbjct: 184 FDSIIFCTGFKRSTHKWLKGDDYLLNDDGLPKPSYPMYWKGKKGLYCVGLSRRGLYGAAA 363
Query: 187 DAVKIAQDI 195
D IA DI
Sbjct: 364 DGENIANDI 390
>AV774936
Length = 477
Score = 99.0 bits (245), Expect = 6e-22
Identities = 48/139 (34%), Positives = 79/139 (56%), Gaps = 1/139 (0%)
Frame = -3
Query: 69 GIKRPSMGPLQLKNTVGKTPVLDIGALEKIRSGDINVVPGIKRINKNGEVELVNGEKLDI 128
GI P GP L+ G+ PV+D+G + +I+ G+I V+P + + +G+
Sbjct: 475 GIPVPPEGPFPLQMKYGQFPVIDVGTVNQIQFGEIQVLPAEIESIRGNPMLFRDGQSQPF 296
Query: 129 DAVVLATGYRSNVPSWLQEGE-FFSKNGYPKMPFPHGWKGNSGLYAVGFTKRGLSGASSD 187
D+++ TG++ + WL+ G+ F ++G+PK P+ WKGN GLY VG ++RG GA D
Sbjct: 295 DSIIFCTGFQRSTKKWLKGGDDFLYEDGFPKPGLPNPWKGNYGLYCVGLSRRGFFGAKLD 116
Query: 188 AVKIAQDIGKVWKQETKQK 206
A +A DI + Q K++
Sbjct: 115 APALAHDIASLITQPEKKE 59
>AV777386
Length = 582
Score = 79.7 bits (195), Expect = 4e-16
Identities = 37/59 (62%), Positives = 47/59 (78%), Gaps = 1/59 (1%)
Frame = -3
Query: 143 SWLQEGEFFS-KNGYPKMPFPHGWKGNSGLYAVGFTKRGLSGASSDAVKIAQDIGKVWK 200
S +Q+ E FS K+G P+ PFP+GWKG++GLYA+GFTKRGL GAS DA +IA+DI WK
Sbjct: 469 SVIQDNEMFSEKDGLPRKPFPNGWKGSNGLYAIGFTKRGLLGASIDAKRIAEDIEHSWK 293
>BP043294
Length = 527
Score = 78.6 bits (192), Expect = 8e-16
Identities = 36/103 (34%), Positives = 62/103 (59%), Gaps = 1/103 (0%)
Frame = -3
Query: 105 VVPGIKRINKNGEVELVNGEKLDIDAVVLATGYRSNVPSWLQEGE-FFSKNGYPKMPFPH 163
V+P + +V +G+ D+++ TG++ + WL+ G+ F +++G+PK P+
Sbjct: 438 VLPAEIESIRGNQVLFRDGKSQPFDSIIFCTGFKRSTKKWLKGGDDFLNEDGFPKPGLPN 259
Query: 164 GWKGNSGLYAVGFTKRGLSGASSDAVKIAQDIGKVWKQETKQK 206
WKGN+GLY VG ++RG GA+ DA IA DI + Q+ K++
Sbjct: 258 HWKGNNGLYCVGLSRRGFFGANMDAQNIANDIASLITQQEKKE 130
>BP074117
Length = 395
Score = 62.8 bits (151), Expect = 5e-11
Identities = 29/96 (30%), Positives = 50/96 (51%), Gaps = 1/96 (1%)
Frame = -3
Query: 114 KNGEVELVNGEKLDIDAVVLATGYRSNVPSWLQEGE-FFSKNGYPKMPFPHGWKGNSGLY 172
+ +V +G+ D+++ TG++ + WL+ G+ ++NG K F WKG +GLY
Sbjct: 375 RGNQVLFGDGKSHTFDSIIFCTGFKRSTQKWLKGGDDLLNENGLTKTNFQSNWKGENGLY 196
Query: 173 AVGFTKRGLSGASSDAVKIAQDIGKVWKQETKQKKQ 208
G ++ G G +A IA DI Q+ K K++
Sbjct: 195 CAGLSRMGFFGVKDEAQNIANDIASSIAQQEKFKQR 88
>TC17820
Length = 584
Score = 57.8 bits (138), Expect = 1e-09
Identities = 26/37 (70%), Positives = 31/37 (83%)
Frame = +3
Query: 180 GLSGASSDAVKIAQDIGKVWKQETKQKKQRTTACHRR 216
GLSGASSDA+KI+QDIG+VWK+ETKQKKQ + R
Sbjct: 3 GLSGASSDAMKISQDIGQVWKEETKQKKQNNNRAYHR 113
>TC14075 homologue to UP|PRP2_MEDTR (Q40375) Repetitive proline-rich cell wall
protein 2 precursor, partial (98%)
Length = 1524
Score = 28.9 bits (63), Expect = 0.73
Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 13/58 (22%)
Frame = -2
Query: 137 YRSNVPSWLQEGEF---------FSKNGYPKMP----FPHGWKGNSGLYAVGFTKRGL 181
Y+ + S+L E E+ F GYP P FP+G GLY GF+ GL
Sbjct: 1277 YQG*LNSYLAETEYRSYAF*K*VFPAGGYPG*PPYGGFPYGGFSTGGLYTGGFSTGGL 1104
>BI418441
Length = 554
Score = 28.9 bits (63), Expect = 0.73
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +1
Query: 115 NGEVELVNGEKLDIDAVVLATGY 137
NG VE + K+D D V+LATG+
Sbjct: 388 NGGVEFDDNTKIDADVVILATGF 456
>TC16202 similar to UP|Q9GFF4 (Q9GFF4) Photosystem II D2 protein
(Fragment), partial (24%)
Length = 667
Score = 28.5 bits (62), Expect = 0.96
Identities = 11/29 (37%), Positives = 21/29 (71%)
Frame = +2
Query: 39 VMMLKWLPLWIVDKLLLILTWFILGDMEK 67
+++L ++ LW + KLLL+L F LG +++
Sbjct: 59 IVILAYVQLWSLSKLLLLLGVFALGSLQE 145
>TC16033 UP|Q7X9C1 (Q7X9C1) NIN-like protein 1, partial (88%)
Length = 2554
Score = 27.7 bits (60), Expect = 1.6
Identities = 33/131 (25%), Positives = 52/131 (39%), Gaps = 2/131 (1%)
Frame = +3
Query: 31 GISTFELAVMMLKWLPLWIVDKLLLILTWFIL--GDMEKYGIKRPSMGPLQLKNTVGKTP 88
G S E + LKW+ + +K +LI W + GD RP + L +
Sbjct: 108 GPSIMEKLIRALKWIKQFNWNKDMLIQIWVPVPRGD-------RPILSANNLPFS----- 251
Query: 89 VLDIGALEKIRSGDINVVPGIKRINKNGEVELVNGEKLDIDAVVLATGYRSNVPSWLQEG 148
LD G+ R +I+ G + + ELV G + +R VP W +
Sbjct: 252 -LDSGSENLARYREIS--EGFQFSAEEDSKELVPG--------LPGRVFRDKVPEWTPDV 398
Query: 149 EFFSKNGYPKM 159
FF + YP++
Sbjct: 399 RFFRSDEYPRV 431
>TC10951 weakly similar to UP|Q93Z11 (Q93Z11) AT5g37360/MNJ8_150, partial
(30%)
Length = 571
Score = 27.7 bits (60), Expect = 1.6
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 44 WLPLWIVDKLLLILTWFI 61
W PLW+V LL +L W I
Sbjct: 77 WFPLWVVHLLLQMLNWQI 24
>TC17127 similar to UP|Q8L751 (Q8L751) AT4g08180/T12G13_20, partial (13%)
Length = 588
Score = 27.3 bits (59), Expect = 2.1
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +3
Query: 90 LDIGALEKIRSGDINVVPGIKRINKNGEVELVNGEKLDID 129
L +G EK+ D + P +R +NGE E+ N EKL ++
Sbjct: 57 LTLGLKEKLPPTDSRLRPD-QRYLENGEYEMANSEKLRLE 173
>TC16506 homologue to UP|Q8VYH4 (Q8VYH4) AT5g15260/F8M21_150, partial (28%)
Length = 652
Score = 27.3 bits (59), Expect = 2.1
Identities = 9/18 (50%), Positives = 16/18 (88%)
Frame = -2
Query: 137 YRSNVPSWLQEGEFFSKN 154
+ ++ PSWL+EGEFF+++
Sbjct: 72 FLTSPPSWLKEGEFFTQS 19
>TC18122 similar to UP|Q8VZY4 (Q8VZY4) 1-aminocyclopropanecarboxylic acid
synthase, partial (36%)
Length = 603
Score = 26.6 bits (57), Expect = 3.6
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +1
Query: 48 WIVDKLLLILTWFILG 63
W++ K+ L+LTWF +G
Sbjct: 226 WVLQKISLLLTWFNIG 273
>TC11148 homologue to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane transport
protein DMT1, partial (33%)
Length = 622
Score = 26.6 bits (57), Expect = 3.6
Identities = 13/45 (28%), Positives = 25/45 (54%)
Frame = +1
Query: 27 REIFGISTFELAVMMLKWLPLWIVDKLLLILTWFILGDMEKYGIK 71
+E+ G S + ++ +PLW + ++ L WFI +E YG++
Sbjct: 478 QEVIG-SAIAIRILSNGVVPLW-AEVVITALDWFIFLFLENYGVR 606
>TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transcription
factor PosF21 (AtbZIP59), partial (17%)
Length = 570
Score = 26.2 bits (56), Expect = 4.7
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -1
Query: 37 LAVMMLKWLPLWIVDKLLLILTWFI 61
L + +L WL W++ LLL+L W +
Sbjct: 219 LLLHLLLWLLYWLLLLLLLLLNWLL 145
>CB827010
Length = 555
Score = 25.8 bits (55), Expect = 6.2
Identities = 15/49 (30%), Positives = 25/49 (50%), Gaps = 2/49 (4%)
Frame = +2
Query: 104 NVVPGIKRINKN--GEVELVNGEKLDIDAVVLATGYRSNVPSWLQEGEF 150
++V ++ I K GE++ E + IDA RSN+ + + GEF
Sbjct: 296 SIVEPVRNIIKKRKGEIKFWEAECVKIDAANKKVSCRSNIDNLVGSGEF 442
>TC18257 weakly similar to UP|Q9LFY5 (Q9LFY5) T7N9.6, partial (46%)
Length = 583
Score = 25.4 bits (54), Expect = 8.1
Identities = 22/71 (30%), Positives = 27/71 (37%)
Frame = +3
Query: 139 SNVPSWLQEGEFFSKNGYPKMPFPHGWKGNSGLYAVGFTKRGLSGASSDAVKIAQDIGKV 198
SN+ S + F GY M WKG S + TKR + A +D K Q V
Sbjct: 336 SNLQSLVVPAAAFGVLGYGYM----WWKGISFSDLMYVTKRNMENAVADLTKKLQHASDV 503
Query: 199 WKQETKQKKQR 209
K QR
Sbjct: 504 IADAKKHLTQR 536
>TC8467 similar to UP|Q8L751 (Q8L751) AT4g08180/T12G13_20, partial (13%)
Length = 665
Score = 25.4 bits (54), Expect = 8.1
Identities = 14/37 (37%), Positives = 21/37 (55%)
Frame = +3
Query: 93 GALEKIRSGDINVVPGIKRINKNGEVELVNGEKLDID 129
G EK+ D + P +R +NGE E+ N EKL ++
Sbjct: 54 GLKEKLPPTDSRLRPD-QRYLENGEYEMANSEKLRLE 161
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,084,601
Number of Sequences: 28460
Number of extensions: 54063
Number of successful extensions: 328
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 323
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 324
length of query: 221
length of database: 4,897,600
effective HSP length: 87
effective length of query: 134
effective length of database: 2,421,580
effective search space: 324491720
effective search space used: 324491720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146819.1