BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146806.4 + phase: 0 /pseudo
(796 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU089582 108 5e-40
AV427422 140 1e-33
TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%) 91 6e-25
BP055130 102 4e-24
BF177840 101 4e-22
BP054462 87 8e-18
BP046863 55 3e-08
BP085845 55 4e-08
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 51 8e-07
TC13302 28 4.3
TC9810 similar to UP|HEM3_PEA (Q43082) Porphobilinogen deaminase... 28 4.3
TC12580 weakly similar to UP|Q8H761 (Q8H761) Polygalacturonase 1... 27 9.7
>AU089582
Length = 383
Score = 108 bits (271), Expect(2) = 5e-40
Identities = 48/76 (63%), Positives = 65/76 (85%)
Frame = +2
Query: 482 ARLAEIYIEQIVRLHGIPSSIVSDRDPRFTSNFWESLQAALGTKLSLSSAYHPQTDGQTE 541
++ A+IY+++IV LHG+P SI+SDR +FTS+FW S Q ALGT+L +S+A+HPQTDGQ+E
Sbjct: 14 SQYAKIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQSE 193
Query: 542 RTIQSLEDLLRACVLE 557
RTIQ LED+LRACV +
Sbjct: 194 RTIQILEDMLRACVXD 241
Score = 73.6 bits (179), Expect(2) = 5e-40
Identities = 32/44 (72%), Positives = 37/44 (83%)
Frame = +3
Query: 561 SWDECLPLIEFTYNNSFHSSIRMAPFEALYGRRCRTPLCWFESG 604
SWD+ L L+EF YNNS+ SSI MAPFEALYGRRCR+P+ WFE G
Sbjct: 252 SWDQYLSLMEFAYNNSYRSSI*MAPFEALYGRRCRSPIGWFEVG 383
>AV427422
Length = 417
Score = 140 bits (352), Expect = 1e-33
Identities = 67/137 (48%), Positives = 93/137 (66%)
Frame = +1
Query: 449 LPKTSKSFDTIWVIVDRLTKSTHFVPIKTSMSIARLAEIYIEQIVRLHGIPSSIVSDRDP 508
LPK SK ++ + V+VDRL+K +HFVP+K + +A+I++ ++VRLHG+P SIVSDRDP
Sbjct: 10 LPK-SKGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDP 186
Query: 509 RFTSNFWESLQAALGTKLSLSSAYHPQTDGQTERTIQSLEDLLRACVLEQGVSWDECLPL 568
F SNFW+ L GTKL +S+AYHP++DGQTE + LE LR + +Q SW +P
Sbjct: 187 LFMSNFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPW 366
Query: 569 IEFTYNNSFHSSIRMAP 585
E+ YN S+H S P
Sbjct: 367 AEYWYNTSYHVSTGQTP 417
>TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%)
Length = 519
Score = 90.5 bits (223), Expect(3) = 6e-25
Identities = 43/78 (55%), Positives = 61/78 (78%)
Frame = +3
Query: 658 VNPVTGVGHALKCRKLTPRFVGPFDVIEKVGVVAYRIALPPSLSNLHNVFHVPKLRKYVH 717
V+P+ GV K KL+PRF+GPF+V+E+VG V+YR+ALPP LS +H VFHV LRKY++
Sbjct: 6 VSPMKGVLRFGKKGKLSPRFIGPFEVLERVGSVSYRLALPPDLSAVHPVFHVSMLRKYLY 185
Query: 718 DASHVIRVDDLEVRDNLT 735
D SHVIR +D+++ +L+
Sbjct: 186 DPSHVIRHEDVQLDVHLS 239
Score = 38.9 bits (89), Expect(3) = 6e-25
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +2
Query: 754 KEIVLVKVIWVGPTGESATWEPESGMMVSYRSCF 787
K++ VKV+W GP+GE ATWE E M Y F
Sbjct: 296 KDVGSVKVLWRGPSGEEATWEAEDIMREKYPHLF 397
Score = 22.3 bits (46), Expect(3) = 6e-25
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 737 ETWPVRIEDRELKRLR 752
++ PV I DR++KRLR
Sbjct: 244 QSTPVAIVDRQVKRLR 291
>BP055130
Length = 567
Score = 102 bits (254), Expect(2) = 4e-24
Identities = 52/107 (48%), Positives = 70/107 (64%)
Frame = +2
Query: 456 FDTIWVIVDRLTKSTHFVPIKTSMSIARLAEIYIEQIVRLHGIPSSIVSDRDPRFTSNFW 515
F I V+VDRL+K HF P + + + +++AE ++ IV+LHG+P +IVSDRD FTS FW
Sbjct: 182 FTVIIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAFW 361
Query: 516 ESLQAALGTKLSLSSAYHPQTDGQTERTIQSLEDLLRACVLEQGVSW 562
+ GT L++SS+YHPQTDGQTE + LE LR V E W
Sbjct: 362 KHFFKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLW 502
Score = 26.6 bits (57), Expect(2) = 4e-24
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = +3
Query: 565 CLPLIEFTYNNSFHSSI 581
CLP E+ YN+SF +SI
Sbjct: 513 CLPWAEYCYNSSFQTSI 563
>BF177840
Length = 410
Score = 101 bits (252), Expect = 4e-22
Identities = 58/131 (44%), Positives = 80/131 (60%), Gaps = 1/131 (0%)
Frame = +2
Query: 500 SSIVSDRDPRFTSNFWESLQAALGTKLSLSSAYHPQTDGQTERTIQSLEDLLRACVLEQG 559
+SIVSDRD +F S+FW +L +GTKL S+ HPQTDGQTE ++L LLR+ VLE+
Sbjct: 11 TSIVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRS-VLERN 187
Query: 560 VS-WDECLPLIEFTYNNSFHSSIRMAPFEALYGRRCRTPLCWFESGESAMLAPEVVQETT 618
+ W+ LP IEF YN HS+ + +PFE +YG TPL + +L + +
Sbjct: 188 LKMWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDLLPMPNTYLLKHKDGKAKA 367
Query: 619 EKVKMIQEKMK 629
E VK + EK+K
Sbjct: 368 EFVKRLHEKIK 400
>BP054462
Length = 422
Score = 87.4 bits (215), Expect = 8e-18
Identities = 48/141 (34%), Positives = 81/141 (57%), Gaps = 1/141 (0%)
Frame = +1
Query: 574 NNSFHSSIRMAPFEALYGRRCRTPLCWFESGESAMLAPEVVQETTEKVKMIQEKMKASQS 633
N++++ S +M+PF+ALYGR L ++ E + ++ + SQ
Sbjct: 1 NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD 180
Query: 634 T*KSYHDKRRKDIEFQVGDHVFLWVNPVTGVGHALKCR-KLTPRFVGPFDVIEKVGVVAY 692
++Y +K+R+D+++Q+GD VFL + P A K KL+PR+ GP+ ++ K+G VAY
Sbjct: 181 MMRTYANKKRRDVDYQIGDEVFLKLQPYRRRSLAKKMNEKLSPRYYGPYPIVAKIGAVAY 360
Query: 693 RIALPPSLSNLHNVFHVPKLR 713
R+ L P+ S +H VFHV L+
Sbjct: 361 RLEL-PAHSRVHPVFHVSLLK 420
>BP046863
Length = 580
Score = 55.5 bits (132), Expect = 3e-08
Identities = 36/103 (34%), Positives = 60/103 (57%), Gaps = 1/103 (0%)
Frame = +1
Query: 613 VVQETTEKVKMIQEKMKASQST*KSYHDKRRKDIEFQVGDHVFLWVNPVTGVGHAL-KCR 671
+++E + ++ + +Q ++ +K R+ +++QVG+ VFL + P A K +
Sbjct: 274 LIEERDALLLELRGNLLKAQDQMRAQANKHRRYVDYQVGNWVFLKLQPYKLQNLAQRKNQ 453
Query: 672 KLTPRFVGPFDVIEKVGVVAYRIALPPSLSNLHNVFHVPKLRK 714
KL+PRF GPF V+E+V VAY + L S S +H VFH+ L K
Sbjct: 454 KLSPRFYGPFKVLERVVQVAY*LDL-XSESRVHPVFHLSLLEK 579
>BP085845
Length = 464
Score = 55.1 bits (131), Expect = 4e-08
Identities = 33/106 (31%), Positives = 57/106 (53%)
Frame = -2
Query: 669 KCRKLTPRFVGPFDVIEKVGVVAYRIALPPSLSNLHNVFHVPKLRKYVHDASHVIRVDDL 728
K RK + +GPF ++E V ++AY + L H V HV L + ++D S I + +
Sbjct: 421 KKRKTESKIIGPFKILEMVCLIAY*LTHSLYLLAAHIVLHVTLLWRNLYDQSQNICHEGV 242
Query: 729 EVRDNLTVETWPVRIEDRELKRLRGKEIVLVKVIWVGPTGESATWE 774
+ ++ + P+ + D +++ +R K I VKVIW G +G +WE
Sbjct: 241 XLGEHWSHMEHPIVMVDMKVRCMRPKNIDDVKVIWRGLSG*EKSWE 104
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 50.8 bits (120), Expect = 8e-07
Identities = 28/90 (31%), Positives = 46/90 (51%)
Frame = +2
Query: 509 RFTSNFWESLQAALGTKLSLSSAYHPQTDGQTERTIQSLEDLLRACVLEQGVSWDECLPL 568
+FTS + +G ++ SS HPQT+GQTE + + ++ + E W + LP+
Sbjct: 20 QFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWIDELPI 199
Query: 569 IEFTYNNSFHSSIRMAPFEALYGRRCRTPL 598
+ ++YN SSI+ PF YG P+
Sbjct: 200 VLWSYNTMPQSSIKETPF*LTYGADTMLPV 289
>TC13302
Length = 533
Score = 28.5 bits (62), Expect = 4.3
Identities = 19/55 (34%), Positives = 26/55 (46%), Gaps = 5/55 (9%)
Frame = -2
Query: 641 KRRKDIEFQVGDHV-----FLWVNPVTGVGHALKCRKLTPRFVGPFDVIEKVGVV 690
+ R+DI+ VGD V L V H KL PRF G +++EK+ V
Sbjct: 241 RHRRDIQLHVGDMV*SNNPTL*TRAYQRVNH-----KLCPRFYGTHEMVEKINPV 92
>TC9810 similar to UP|HEM3_PEA (Q43082) Porphobilinogen deaminase,
chloroplast precursor (PBG) (Hydroxymethylbilane
synthase) (HMBS) (Pre-uroporphyrinogen synthase) ,
partial (44%)
Length = 884
Score = 28.5 bits (62), Expect = 4.3
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = -3
Query: 476 KTSMSIARLAEIYIEQIVRLHGIPSSIVSDRDPRFTSNFWESLQ 519
+TSM+ + L + I Q+ + +GIP S + DP S F S Q
Sbjct: 753 ETSMNSSELTIVEIIQVTKKNGIPISKIQHFDPNERSTFSISSQ 622
>TC12580 weakly similar to UP|Q8H761 (Q8H761) Polygalacturonase 1, partial
(7%)
Length = 714
Score = 27.3 bits (59), Expect = 9.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1
Query: 654 VFLWVNPVTGVGHALKCRKLTP 675
+ L++ P+T GH L C LTP
Sbjct: 207 ILLFITPITRRGHVLDCLLLTP 142
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.340 0.151 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,786,580
Number of Sequences: 28460
Number of extensions: 168487
Number of successful extensions: 1141
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1137
length of query: 796
length of database: 4,897,600
effective HSP length: 98
effective length of query: 698
effective length of database: 2,108,520
effective search space: 1471746960
effective search space used: 1471746960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146806.4