BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146794.16 + phase: 0 /pseudo/partial
(1323 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG662087 132 4e-31
BP065719 64 2e-10
TC18698 57 1e-08
AV410603 54 2e-07
AV427333 38 0.009
TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein p... 35 0.10
AU251550 34 0.13
TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycop... 34 0.13
TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protei... 33 0.39
TC14977 similar to UP|CAE45567 (CAE45567) SUMO E2 conjugating en... 32 0.50
TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycop... 32 0.50
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 32 0.50
TC12396 similar to UP|Q940Z6 (Q940Z6) At1g12810/F13K23_4, partia... 32 0.50
BG662122 32 0.66
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 32 0.66
TC19101 weakly similar to UP|Q93WC9 (Q93WC9) Adenylate isopenten... 31 1.1
TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase ... 31 1.1
TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich... 31 1.5
AV409168 28 1.9
TC17405 similar to UP|Q940D1 (Q940D1) At1g64110/F22C12_22, parti... 30 1.9
>BG662087
Length = 373
Score = 132 bits (332), Expect = 4e-31
Identities = 63/119 (52%), Positives = 84/119 (69%)
Frame = +1
Query: 1101 GKVRMCVDFRDLNKASLKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNQIKMSPEDREK 1160
GK RM VD+ DLNKA KD++PLP ID LVD + +++ S MD +SGY+QIKM P D +K
Sbjct: 16 GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDK 195
Query: 1161 TSFITPWGTFCYKVMPFGLINAGATYQRGMTTLFHDMIHKEVEVYVDDMIVKSTDEEQH 1219
T+F+T +CY+ +PFGL NAGATYQ M +F D + + +EVY+D+MIVKS H
Sbjct: 196 TAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKSALRANH 372
>BP065719
Length = 567
Score = 63.9 bits (154), Expect = 2e-10
Identities = 39/153 (25%), Positives = 76/153 (49%), Gaps = 2/153 (1%)
Frame = +3
Query: 9 RHEIKANRGNADSFKTQDLCLVSKVDVPKKFKIPDFDRYNGLTCPQN--HIIKYVRKMGN 66
+H N F + + V + ++P+ +K+P F +++G + HI +Y + G+
Sbjct: 84 QHGFNVGFANRPHFVSAFIEEVLESELPRGWKVPKFTKFSGDSGESTVEHIARYQIEAGD 263
Query: 67 YKDNDSLMIHCFQDSLMEDAAEWYTSLSKNDIHTFDELAVAFKSHYGFNTRLKPNREFLR 126
N++L + F SL ++A W+T+L+ +HT+ +L F + F K + + L
Sbjct: 264 LAINENLKMKYFPSSLTKNAFTWFTTLAPRSVHTWAQLERIFHEQF-FRGECKVSXKDLA 440
Query: 127 SLSQKKEESFREYAQRWRGAAARITPALDEEEM 159
S+ +K ES +Y R+R +R + E E+
Sbjct: 441 SVKRKPAESIDDYLNRFRMLKSRCFTHVSEHEL 539
>TC18698
Length = 808
Score = 57.4 bits (137), Expect = 1e-08
Identities = 31/62 (50%), Positives = 39/62 (62%)
Frame = -2
Query: 1158 REKTSFITPWGTFCYKVMPFGLINAGATYQRGMTTLFHDMIHKEVEVYVDDMIVKSTDEE 1217
++KT+ + Y+VMP GL N TYQR M +FH I K VEVYV+DMIVKS+ E
Sbjct: 807 KKKTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKSSQE* 628
Query: 1218 QH 1219
H
Sbjct: 627 FH 622
>AV410603
Length = 162
Score = 53.9 bits (128), Expect = 2e-07
Identities = 26/54 (48%), Positives = 37/54 (68%)
Frame = +1
Query: 1115 ASLKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNQIKMSPEDREKTSFITPWG 1168
+++KD+FP+P +D L+D S+ FS +D SGY+QI + PEDR KT F T G
Sbjct: 1 STVKDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>AV427333
Length = 387
Score = 38.1 bits (87), Expect = 0.009
Identities = 30/99 (30%), Positives = 36/99 (36%), Gaps = 6/99 (6%)
Frame = +3
Query: 252 PPSYPYVPYS--QHPFFPP----FYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQQNQQ 305
PPS+PY P + HPF PP Y + P PP P P
Sbjct: 87 PPSHPYNPPTPPSHPFNPPPTPSHYFKSPPPPSHSFAP-----PPRGHPSPPPSSPPPPS 251
Query: 306 ARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPPPDPLPP 344
+ P PP P + P H +PPP PLPP
Sbjct: 252 SHPFTPPPPHVRPPPSP---HHPITPPPHVRPPPPPLPP 359
>TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (87%)
Length = 1232
Score = 34.7 bits (78), Expect = 0.10
Identities = 25/91 (27%), Positives = 43/91 (46%), Gaps = 3/91 (3%)
Frame = +3
Query: 247 NAAQMPPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQQNQQA 306
N + S+ +P+S H PP PLP + Q+ ++ +A+ Q+ P +++ +
Sbjct: 30 NGSSFFTSHILLPHSHH-HHPPHTFTCPLPNPRQQLQIHRLARHPAQRRPPSPRRRRLRP 206
Query: 307 RPTFPPI-PMLYAELLPTLL--HRGHCTTRQ 334
+P PP+ P L+ L HR H RQ
Sbjct: 207 QPLHPPLHPRPRHSLVRPRLGPHRLHLRRRQ 299
>AU251550
Length = 329
Score = 34.3 bits (77), Expect = 0.13
Identities = 18/40 (45%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +2
Query: 252 PPSYPYVPYSQHPFFPPFYHQYPLPP-GQPQVPVNAIAQQ 290
PP P PY+ P PPF H +P PP G P P A+ Q
Sbjct: 140 PPGPPRDPYAPAPPPPPFGHHHPPPPHGPPSPPPPAMFYQ 259
>TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycoprotein
DZ-HRGP precursor, partial (8%)
Length = 584
Score = 34.3 bits (77), Expect = 0.13
Identities = 23/76 (30%), Positives = 28/76 (36%)
Frame = +1
Query: 255 YPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQQNQQARPTFPPIP 314
YP +PY QH F+PP P PP P P A P + T PP P
Sbjct: 202 YPSLPYPQHQFYPPPMAPPPPPPPAPAPP----APPPYWAAPPPRPYGPPLFTSTLPPRP 369
Query: 315 MLYAELLPTLLHRGHC 330
A + + HC
Sbjct: 370 PKPAPPKNVVKEKSHC 417
>TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protein, partial
(25%)
Length = 1077
Score = 32.7 bits (73), Expect = 0.39
Identities = 33/109 (30%), Positives = 38/109 (34%)
Frame = +1
Query: 238 QPMATVALINAAQMPPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPV 297
QP + L PPS P P +PF PP P PP P +P P
Sbjct: 553 QPPSPPPLFPNPFQPPSPP--PLIPNPFQPP-----PSPP--PFIPN-----------PF 672
Query: 298 QQQQQNQQARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPPPDPLPPRF 346
Q Q P FP P++ L P PPP P PP F
Sbjct: 673 QPPPSKQPPSPLFPFPPIVIPGLTP--------------PPPPPPPPPF 777
Score = 29.3 bits (64), Expect = 4.3
Identities = 15/31 (48%), Positives = 18/31 (57%)
Frame = +1
Query: 252 PPSYPYVPYSQHPFFPPFYHQYPLPPGQPQV 282
PP P P PFFPPF +P PPG+P +
Sbjct: 748 PPPPPPPP----PFFPPFPPLFP-PPGRPPI 825
>TC14977 similar to UP|CAE45567 (CAE45567) SUMO E2 conjugating enzyme SCE1,
partial (98%)
Length = 871
Score = 32.3 bits (72), Expect = 0.50
Identities = 26/85 (30%), Positives = 35/85 (40%), Gaps = 1/85 (1%)
Frame = +1
Query: 869 GRLIQLRENKHKEGLGFSPTSGVSTGAFYSAGFVNAITEEATGFGPRPVFVIPGGIARDW 928
GRL + R++ K P V+ S G +N + T IPG DW
Sbjct: 148 GRLTEERKSWRKN----HPHGFVAKPETLSDGTINLMVWHCT---------IPGKTGTDW 288
Query: 929 DAIDIPSIMHVS-DNPAFPPNFEFP 952
D P +H S D P+ PP +FP
Sbjct: 289 DGGYFPLTLHFSEDYPSKPPKCKFP 363
>TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (57%)
Length = 941
Score = 32.3 bits (72), Expect = 0.50
Identities = 25/94 (26%), Positives = 33/94 (34%), Gaps = 2/94 (2%)
Frame = +1
Query: 252 PPSYPYVPYSQHPFFPPFYHQYPLPPG-QPQVPVNAIAQQMKQQLPVQQQQQNQQARPT- 309
PP P Y + P PP+Y++ P PP P P + P P+
Sbjct: 31 PPPVPKPYYYKSPPPPPYYYKSPPPPSPSPPPPYYYQSPPPPSHSPPPPYYYKSPPPPSP 210
Query: 310 FPPIPMLYAELLPTLLHRGHCTTRQGKPPPDPLP 343
PP P Y P + PPP P+P
Sbjct: 211 SPPPPYYYKSPPPPSPSPPPPYYYKSPPPPSPIP 312
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 32.3 bits (72), Expect = 0.50
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +2
Query: 280 PQVPVNAIAQQMKQQLPVQQQQQNQQARP--TFPPIPMLYAELLP 322
PQV ++Q +QQLP QQQQ Q +P P + L + LP
Sbjct: 92 PQVQQQQLSQMQQQQLPQLQQQQLSQLQPQQQLPQLQQLQHQQLP 226
>TC12396 similar to UP|Q940Z6 (Q940Z6) At1g12810/F13K23_4, partial (39%)
Length = 408
Score = 32.3 bits (72), Expect = 0.50
Identities = 35/127 (27%), Positives = 46/127 (35%), Gaps = 8/127 (6%)
Frame = +3
Query: 248 AAQMPPS---YPYVPYSQHPFFPP--FYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQQ 302
A+Q+P + Y P +P +PP + YP P G P Q P
Sbjct: 30 ASQIPQTMSNYQRAPQEPYPDYPPPGYGSPYPPPQGYP-------VTQPPPGYPSAPPPP 188
Query: 303 NQQARPTFPPIPMLYAELLPTLLHRGH-CTTRQGKP--PPDPLPPRFRSDLKCDFHQGAL 359
+ P PP P +YA + G+ QG P PP P PP + H
Sbjct: 189 GYEGYP--PPPPPVYAGYAARPQYEGYQGYFNQGYPQQPPPPPPPGYHC-----HHDQHH 347
Query: 360 GHDVEGC 366
HD GC
Sbjct: 348 HHDSPGC 368
>BG662122
Length = 386
Score = 32.0 bits (71), Expect = 0.66
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +2
Query: 329 HCTTRQGKPPPDPLPPRFRSDLK--CDFHQGALGHDVEGCYALKYIVKKLIDQGKL 382
H + G+ P PPR D C++ + + HD++ C+ LK ++KLI G+L
Sbjct: 146 HMVGKSGQS*P---PPRRGIDTTK*CEYRRSVV-HDIDDCFTLKREIEKLIKMGRL 301
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 32.0 bits (71), Expect = 0.66
Identities = 27/94 (28%), Positives = 32/94 (33%)
Frame = -1
Query: 252 PPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQQNQQARPTFP 311
PPS PY P + P PP YP PP P P + P + P +P
Sbjct: 506 PPSPPYPPSRRPP*PPPPPPPYPPPP--PP*P--------RDPPPPPYPPSRRPPPPPYP 357
Query: 312 PIPMLYAELLPTLLHRGHCTTRQGKPPPDPLPPR 345
P P +PPP P PPR
Sbjct: 356 PPP---------------------RPPPPPPPPR 318
>TC19101 weakly similar to UP|Q93WC9 (Q93WC9) Adenylate
isopentenyltransferase (Cytokinin synthase)
(AT3g63110/T20O10_210) , partial (37%)
Length = 880
Score = 31.2 bits (69), Expect = 1.1
Identities = 23/79 (29%), Positives = 31/79 (39%), Gaps = 9/79 (11%)
Frame = +2
Query: 271 HQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQQNQQARPTFPPIP---------MLYAELL 321
H +PL Q + Q + P + QQN Q R T+ P P L+
Sbjct: 521 HLFPLRNHQ-------LRQDSNLRRPRHRHQQNLQGRTTWNPPPPPRNSKP*HRLHRRRF 679
Query: 322 PTLLHRGHCTTRQGKPPPD 340
P L HR H + +PP D
Sbjct: 680 P*LFHRRH*RNHKPRPPSD 736
>TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase F8D20.150
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (24%)
Length = 775
Score = 31.2 bits (69), Expect = 1.1
Identities = 27/100 (27%), Positives = 42/100 (42%), Gaps = 11/100 (11%)
Frame = +3
Query: 252 PPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQL-------PVQQQQQNQ 304
PP P ++ P+F P H PP P +P +A+ + L P +++
Sbjct: 270 PPPPPPHHHNSLPYFNPTSHGLCEPPPPPPLPPP*LARSPQHALRRRPPPPPPPKRRS*T 449
Query: 305 QARPTFPPIPMLYAELLPTLLHRGHC----TTRQGKPPPD 340
+ P + + + E LP L H G C TR G+ PD
Sbjct: 450 RPPPPYSRLRLPPRESLPCLRH-GSCRPGTRTRLGRTRPD 566
>TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich protein 1
(NDPP-1). {Mus musculus;}, partial (8%)
Length = 1134
Score = 30.8 bits (68), Expect = 1.5
Identities = 29/99 (29%), Positives = 33/99 (33%), Gaps = 2/99 (2%)
Frame = +3
Query: 248 AAQMPPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQQNQQAR 307
A Q P SY S PF +H YPL A A P
Sbjct: 45 ATQTPSSYS----STLPFTNKSHHHYPLT-------TTASASSSSSSSPSSS*PSPSLPP 191
Query: 308 PTFPPIPMLYAELLPTLL--HRGHCTTRQGKPPPDPLPP 344
P+ PP+P A LP+ L T PPP P P
Sbjct: 192 PSPPPLPPPQAPPLPSHLPSPEPSSTMPPSTPPPQPTKP 308
>AV409168
Length = 243
Score = 27.7 bits (60), Expect(2) = 1.9
Identities = 16/44 (36%), Positives = 23/44 (51%), Gaps = 3/44 (6%)
Frame = +3
Query: 268 PFYHQYPLPPGQ---PQVPVNAIAQQMKQQLPVQQQQQNQQARP 308
P H + +P Q P P + I+ Q+KQQ QQ QQ ++ P
Sbjct: 18 PVNHGFQIPKNQQQPPPPPPSPISSQLKQQ---QQHQQQWRSNP 140
Score = 21.2 bits (43), Expect(2) = 1.9
Identities = 8/18 (44%), Positives = 9/18 (49%)
Frame = +2
Query: 327 RGHCTTRQGKPPPDPLPP 344
R H + P PDP PP
Sbjct: 146 RRHPRRTRQNPSPDPTPP 199
>TC17405 similar to UP|Q940D1 (Q940D1) At1g64110/F22C12_22, partial (10%)
Length = 522
Score = 30.4 bits (67), Expect = 1.9
Identities = 15/43 (34%), Positives = 24/43 (54%), Gaps = 4/43 (9%)
Frame = +3
Query: 241 ATVALINAAQMPPSYPYVPYS----QHPFFPPFYHQYPLPPGQ 279
A+ +I A +PP YP P+S Q+P F +Q+ +P G+
Sbjct: 42 ASYNIIAAISLPPQYPVYPFSLNLLQNPLNHSFSYQFLIPFGE 170
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.137 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,305,239
Number of Sequences: 28460
Number of extensions: 326175
Number of successful extensions: 2340
Number of sequences better than 10.0: 71
Number of HSP's better than 10.0 without gapping: 2173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2302
length of query: 1323
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1222
effective length of database: 2,023,140
effective search space: 2472277080
effective search space used: 2472277080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146794.16