BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146789.8 + phase: 0 /pseudo
(2263 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI417841 63 6e-10
TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial ... 55 2e-07
BP058026 37 0.045
BP082711 35 0.13
BG662087 34 0.29
BP029370 32 1.1
TC12062 weakly similar to UP|Q9WSV7 (Q9WSV7) DNA, complete genom... 32 1.4
AU089225 31 1.9
TC15036 similar to UP|Q8LKG1 (Q8LKG1) Drm3, partial (95%) 30 4.2
AV418552 30 4.2
TC7897 weakly similar to UP|ENL2_ARATH (Q9T076) Early nodulin-li... 30 5.4
TC15728 similar to AAS09986 (AAS09986) MYB transcription factor,... 30 5.4
TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial ... 30 5.4
BP029871 27 7.5
TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%) 29 9.3
AW720177 29 9.3
TC17842 similar to PIR|B86422|B86422 F1N18.10 protein - Arabidop... 29 9.3
BG662279 29 9.3
TC17205 weakly similar to PIR|S50830|S50830 Machado-Joseph disea... 29 9.3
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 29 9.3
>BI417841
Length = 617
Score = 62.8 bits (151), Expect = 6e-10
Identities = 34/109 (31%), Positives = 58/109 (53%)
Frame = +1
Query: 1705 GIGAVLLTPKGAHIPFTARLRFDCTNNIAEYEACIMGIEEAIDLRIKNIEIYGDSALVIN 1764
G GAVL G+ + + + TNN AEY I+G++ A + ++I + GDS LV
Sbjct: 184 GAGAVLRAEDGSKVYLREGVG-NQTNNQAEYRGLILGLKHAHEQGYQHINVKGDSQLVCK 360
Query: 1765 QIKGKWETLHAGLIPYRDYARRLLTFFNKVELHHIPRDENQMADALATV 1813
Q++G W+ + + + A+ L + F +++H+PR N AD A +
Sbjct: 361 QVEGSWKARNPNIASLCNEAKELKSKFQSFDINHVPRQYNSEADVQANL 507
>TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (11%)
Length = 632
Score = 54.7 bits (130), Expect = 2e-07
Identities = 27/77 (35%), Positives = 43/77 (55%)
Frame = +2
Query: 1735 YEACIMGIEEAIDLRIKNIEIYGDSALVINQIKGKWETLHAGLIPYRDYARRLLTFFNKV 1794
Y I+G++ AI K+I++ GDS LV NQ++G W+ + + A+ L F
Sbjct: 2 YRGLILGLKHAIKEGYKHIQVKGDSMLVCNQVQGLWKIKNQNIASLCSEAKELKNKFLSF 181
Query: 1795 ELHHIPRDENQMADALA 1811
+++HIPR+ N AD A
Sbjct: 182 KINHIPREYNSEADVQA 232
>BP058026
Length = 389
Score = 36.6 bits (83), Expect = 0.045
Identities = 14/27 (51%), Positives = 20/27 (73%)
Frame = +3
Query: 436 ILNNHTNNNHTNNSHTNNTLNKISNNN 462
I+NN+ NNN+ NN++ NN N +NNN
Sbjct: 3 IINNNNNNNNNNNNNNNNNNNNNNNNN 83
Score = 35.8 bits (81), Expect = 0.076
Identities = 15/29 (51%), Positives = 21/29 (71%)
Frame = +3
Query: 436 ILNNHTNNNHTNNSHTNNTLNKISNNNLI 464
I NN+ NNN+ NN++ NN N +NN+LI
Sbjct: 6 INNNNNNNNNNNNNNNNNNNNNNNNNDLI 92
Score = 31.6 bits (70), Expect = 1.4
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +3
Query: 442 NNNHTNNSHTNNTLNKISNNNLINN 466
NNN+ NN++ NN N +NNN NN
Sbjct: 9 NNNNNNNNNNNNNNNNNNNNNNNNN 83
>BP082711
Length = 379
Score = 35.0 bits (79), Expect = 0.13
Identities = 25/87 (28%), Positives = 42/87 (47%), Gaps = 5/87 (5%)
Frame = +3
Query: 427 NSHKILHHKILNNHTNNNHTNNSHTNNTLNKISNNNLINNAHNNLDHQECRLTQYQSPMQ 486
N + +LH++ + NNN+ NN++ NN NNN NN + + D + L + S
Sbjct: 132 NIYPVLHYRARHLFYNNNNNNNNNNNN------NNNNNNNKNIS*DSPKLLLMSHISTSS 293
Query: 487 NYFPAYSRKTLS-----KLEQLLLSQK 508
P + R +S K+ +L+L K
Sbjct: 294 T*QPDHLRDNVSLA*PKKMIELILHSK 374
>BG662087
Length = 373
Score = 33.9 bits (76), Expect = 0.29
Identities = 28/77 (36%), Positives = 37/77 (47%)
Frame = +2
Query: 1286 CVLTTVI*TKLAQKTTFLYLILTCWLTVLPSLKSSHSWMDSPVIIKSRWHPKIERKHHLL 1345
C TT I*T+ AQKT + T W P++ WM + VI KSR ++ K
Sbjct: 29 CGWTTRI*TRHAQKTLIHCQV*TNWWMERPTMSC*A*WMPTQVITKSRCTHQMRTKRPS* 208
Query: 1346 HLGALFATK*CRSV*SM 1362
G + AT+ RS * M
Sbjct: 209 PPG*ITATRQFRSG*RM 259
>BP029370
Length = 364
Score = 32.0 bits (71), Expect = 1.1
Identities = 19/54 (35%), Positives = 33/54 (60%), Gaps = 3/54 (5%)
Frame = +1
Query: 422 YHNILNSHKILHHKILNNHTNNNHTNNSHTNNT--LNKISNN-NLINNAHNNLD 472
Y+ I++ +ILH + N+ NNN+ NN++ NN + KI+N+ +N H+ D
Sbjct: 124 YNIIISYKRILHII*IYNNNNNNNKNNNNNNNNSII*KITNHITYASNFHHLWD 285
>TC12062 weakly similar to UP|Q9WSV7 (Q9WSV7) DNA, complete genome,
isolate:TTV SANBAN, partial (4%)
Length = 578
Score = 31.6 bits (70), Expect = 1.4
Identities = 33/97 (34%), Positives = 40/97 (41%), Gaps = 20/97 (20%)
Frame = -3
Query: 12 HRFKHKRKH--WHKRKLKLRP*QKRRPDH------KHLHLLLSELRPRHLLLGHYVLTL- 62
HR K +R H W RK R +KRR D K S L+PRHLLL L L
Sbjct: 420 HRNKSRRSHRRWRNRKRPRRRSKKRRVDRTTRKQKKQKPTGQSRLQPRHLLLQSRQLGLL 241
Query: 63 -----------QRSPLRNVPRLGFHLSLLGRYFVLSL 88
+RS R P +G H +G +L L
Sbjct: 240 FRHRRRRRLRRRRSGRRQRPCVG-HRRCIGELVLLHL 133
>AU089225
Length = 651
Score = 31.2 bits (69), Expect = 1.9
Identities = 15/59 (25%), Positives = 32/59 (53%), Gaps = 1/59 (1%)
Frame = +2
Query: 382 NKLIQPINTLPPSHPPLTLFNKQITILKY-RNILKYRNILKYHNILNSHKILHHKILNN 439
N+L++ + L P+ + T+LK RN+++Y + KY + ++ ++ I+NN
Sbjct: 71 NRLLKALEVLKSEVSPMQTASILQTLLKVIRNVIEYPELEKYKRLRKTNPVIERDIVNN 247
>TC15036 similar to UP|Q8LKG1 (Q8LKG1) Drm3, partial (95%)
Length = 803
Score = 30.0 bits (66), Expect = 4.2
Identities = 13/40 (32%), Positives = 22/40 (54%)
Frame = -3
Query: 429 HKILHHKILNNHTNNNHTNNSHTNNTLNKISNNNLINNAH 468
H++LHH +++ HT++ T +S N +S N AH
Sbjct: 594 HELLHHNLISIHTDSYKTESSKGQKLTNILSFRRRENVAH 475
>AV418552
Length = 386
Score = 30.0 bits (66), Expect = 4.2
Identities = 14/35 (40%), Positives = 24/35 (68%), Gaps = 1/35 (2%)
Frame = +3
Query: 437 LNNHTNNNHTNNSHTNNTLN-KISNNNLINNAHNN 470
L++ NN+ + N H +N+ N SNNN ++N++NN
Sbjct: 162 LHSSNNNSRSCNLHCSNSSN*HSSNNNNLHNSNNN 266
>TC7897 weakly similar to UP|ENL2_ARATH (Q9T076) Early nodulin-like protein
2 precursor (Phytocyanin-like protein), partial (31%)
Length = 1482
Score = 29.6 bits (65), Expect = 5.4
Identities = 26/74 (35%), Positives = 34/74 (45%), Gaps = 2/74 (2%)
Frame = +1
Query: 6 LRLKLKHRFKHKRKHWHKRKLKLRP*QKRRPDHKHLHLLLSELRP--RHLLLGHYVLTLQ 63
LR +L + R+ R + RP R H HLHL LR RH L H+ L L+
Sbjct: 619 LRQELLRLYCLLRRELQYRPVDRRPELLRLHQHLHLHLR*VPLRRLLRHRELNHH-LRLR 795
Query: 64 RSPLRNVPRLGFHL 77
++ LR V HL
Sbjct: 796 QATLRRVDLRRLHL 837
>TC15728 similar to AAS09986 (AAS09986) MYB transcription factor, partial
(34%)
Length = 1207
Score = 29.6 bits (65), Expect = 5.4
Identities = 17/42 (40%), Positives = 24/42 (56%)
Frame = +3
Query: 4 RKLRLKLKHRFKHKRKHWHKRKLKLRP*QKRRPDHKHLHLLL 45
R+ R +L H +H+R+ W +R LR R H+ LHLLL
Sbjct: 210 RQQRPQLPHMHRHRRR-WRQRHHALR-----RAPHRRLHLLL 317
>TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial (7%)
Length = 841
Score = 29.6 bits (65), Expect = 5.4
Identities = 20/73 (27%), Positives = 35/73 (47%)
Frame = +2
Query: 418 NILKYHNILNSHKILHHKILNNHTNNNHTNNSHTNNTLNKISNNNLINNAHNNLDHQECR 477
NI++ ++ +H N +N NH N S NT + + N+NL +N +NL+H
Sbjct: 74 NIIRIFSLTPNHSYSLSPNPNTFSNLNH-NLSPNPNTFSNL-NHNLSSNTFSNLNHNRNF 247
Query: 478 LTQYQSPMQNYFP 490
S +++ P
Sbjct: 248 RINIFSTLEHILP 286
>BP029871
Length = 519
Score = 27.3 bits (59), Expect(2) = 7.5
Identities = 17/75 (22%), Positives = 32/75 (42%), Gaps = 4/75 (5%)
Frame = +3
Query: 429 HKILHHKILNNHTNNNHTNNSHTNNTLNKISNNNLINNAHNNLD----HQECRLTQYQSP 484
H LHHK NNN + N + + L +D + L + +S
Sbjct: 267 HHPLHHKDEEKEENNNTVQPNPMNFQIERDQRQQLPQLQQQQVDPWTNDEVLALLRIRSS 446
Query: 485 MQNYFPAYSRKTLSK 499
M+++FP ++ + +S+
Sbjct: 447 MESWFPEFTWEHVSR 491
Score = 20.0 bits (40), Expect(2) = 7.5
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = +1
Query: 381 LNKLIQPINTLPPS 394
L+ L+QP+ PPS
Sbjct: 166 LSMLLQPLTITPPS 207
>TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%)
Length = 1231
Score = 28.9 bits (63), Expect = 9.3
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = -3
Query: 42 HLLLSELRPRHLLLGHYVLTLQRSPLRNVPRLGFHLSLLGRYFVLSLVKH 91
H L +L HLLL L L +++ P LG HLS VL L+ H
Sbjct: 506 HFDLLKLGDYHLLLRLLFLLLLTKKIKDTPVLGLHLSGTTLILVLVLLCH 357
>AW720177
Length = 505
Score = 28.9 bits (63), Expect = 9.3
Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 7/73 (9%)
Frame = +2
Query: 7 RLKLKHRFKHKRKHWHKRK----LKLRP*QKRRPD---HKHLHLLLSELRPRHLLLGHYV 59
RL+ F +R++ H+ K + RP Q +P H+ L + LR + L
Sbjct: 107 RLRSPSNFPPRRRNPHRPKPQNQVVTRPSQTHQPPNPPHRFLRRVSLLLRHQPTLSRPLP 286
Query: 60 LTLQRSPLRNVPR 72
L L+ SPLR +PR
Sbjct: 287 LPLRHSPLRRLPR 325
>TC17842 similar to PIR|B86422|B86422 F1N18.10 protein - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (6%)
Length = 445
Score = 28.9 bits (63), Expect = 9.3
Identities = 11/35 (31%), Positives = 21/35 (59%)
Frame = +3
Query: 436 ILNNHTNNNHTNNSHTNNTLNKISNNNLINNAHNN 470
I NN+ NNN+ +S ++ + +NN+L + H +
Sbjct: 30 IFNNNNNNNNAPSSTNSSMYSHYNNNSLFQHQHQH 134
>BG662279
Length = 337
Score = 28.9 bits (63), Expect = 9.3
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +1
Query: 1301 TFLYLILTCWLTVLPSLKSSHSWMDSP 1327
+F Y I+T W T + KS+H+W P
Sbjct: 43 SFSYSIITTWRTCTSTTKSNHTWFCYP 123
>TC17205 weakly similar to PIR|S50830|S50830 Machado-Joseph disease MJD1a
protein - human {Homo sapiens;}, partial (7%)
Length = 528
Score = 28.9 bits (63), Expect = 9.3
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Frame = +1
Query: 420 LKYHNILNSHKILHHKILNNHTN----NNHTNNSHTNNTLNKISNNNLINNAHNNLD 472
L +H+ + S+ HH + H + + ++ TNN + +NNN N A +N++
Sbjct: 211 LHHHSQMLSYATTHHDNTDTHIQQPIKHGYPYSAKTNNNNSNGTNNNSNNKAQSNIN 381
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 28.9 bits (63), Expect = 9.3
Identities = 14/40 (35%), Positives = 27/40 (67%), Gaps = 2/40 (5%)
Frame = +3
Query: 433 HHKILNNHTNNNHTNNSHTNNTLN--KISNNNLINNAHNN 470
HH + ++ NNN ++N H +N+ + + S+NN +N ++NN
Sbjct: 48 HHNLPSS--NNNSSSNFHKSNSSSFPRCSSNNSLNFSNNN 161
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.357 0.157 0.557
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,886,720
Number of Sequences: 28460
Number of extensions: 605201
Number of successful extensions: 8179
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 5706
Number of HSP's successfully gapped in prelim test: 213
Number of HSP's that attempted gapping in prelim test: 1797
Number of HSP's gapped (non-prelim): 6602
length of query: 2263
length of database: 4,897,600
effective HSP length: 105
effective length of query: 2158
effective length of database: 1,909,300
effective search space: 4120269400
effective search space used: 4120269400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146789.8