BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146784.5 + phase: 0
(283 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV408330 70 5e-13
BP046076 69 9e-13
TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%) 69 1e-12
TC16534 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcri... 68 2e-12
TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcri... 67 3e-12
TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding prot... 67 3e-12
TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcrip... 65 1e-11
TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcri... 62 8e-11
AV423663 62 8e-11
AV420160 61 2e-10
TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-bi... 60 3e-10
BP045724 60 4e-10
TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcrip... 59 7e-10
TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%) 59 1e-09
TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcri... 57 3e-09
AV427157 56 8e-09
TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription fact... 55 2e-08
TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcrip... 54 2e-08
TC11031 homologue to UP|WR65_ARATH (Q9LP56) Probable WRKY transc... 48 2e-06
TC8298 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtWRK... 40 4e-04
>AV408330
Length = 316
Score = 69.7 bits (169), Expect = 5e-13
Identities = 36/99 (36%), Positives = 56/99 (56%), Gaps = 1/99 (1%)
Frame = +3
Query: 83 KSEDSLESNCKSSIVKERRGCYKRRKISQTWEKESEQPE-EDGHQWRKYGQKKILHTDFP 141
++ED + K + ++ ++R+ + +SE EDG++WRKYGQK + ++ FP
Sbjct: 9 QTEDDEHNKTKKQLKAKKTNQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFP 188
Query: 142 RNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHHT 180
R+YYRCT C K+V+R DP + TTY G HT
Sbjct: 189 RSYYRCT---SASCNVKKRVERSFTDPSIVVTTYEGQHT 296
>BP046076
Length = 593
Score = 68.9 bits (167), Expect = 9e-13
Identities = 37/87 (42%), Positives = 52/87 (59%)
Frame = -2
Query: 93 KSSIVKERRGCYKRRKISQTWEKESEQPEEDGHQWRKYGQKKILHTDFPRNYYRCTHKND 152
+ S VK RR + R QT + +DG++WRKYGQK + ++ PR+YYRCTH N
Sbjct: 538 EKSKVKVRRKLREPRFCFQT--RSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSN- 368
Query: 153 QGCKAIKQVQRIQEDPPLHRTTYSGHH 179
C+ K+V+R+ ED + TTY G H
Sbjct: 367 --CRVKKRVERLSEDCRMVITTYEGRH 293
>TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%)
Length = 617
Score = 68.6 bits (166), Expect = 1e-12
Identities = 41/103 (39%), Positives = 57/103 (54%), Gaps = 1/103 (0%)
Frame = +1
Query: 78 DMQLTKSEDSLESNCKSSIVKERRGCYKRR-KISQTWEKESEQPEEDGHQWRKYGQKKIL 136
D LT S S C S K+ R KR ++ K ++ P +D QWRKYGQK I
Sbjct: 43 DATLTGKTSSSGSRCHCSKKKKLR--VKRTIRVPAISSKTADIPTDD-FQWRKYGQKPIK 213
Query: 137 HTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
+ +PR YY+C+ +++GC A K V+R Q+DP + TY G H
Sbjct: 214 GSPYPRGYYKCS--SEKGCPARKHVERAQDDPNMLIVTYEGEH 336
>TC16534 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcription
factor 41 (WRKY DNA-binding protein 41), partial (20%)
Length = 697
Score = 68.2 bits (165), Expect = 2e-12
Identities = 49/157 (31%), Positives = 71/157 (45%), Gaps = 13/157 (8%)
Frame = +1
Query: 14 VVDELLRGRELANKLRNILNESGDIYNNDGST--IPPFAEDLLKEVLTTFTNSLLFLNNN 71
+++EL++G E+A +L+ L + D I E L+ + + S L N
Sbjct: 223 LINELIQGMEVARRLKEDLKTPYSVNTRDLQVQRILSSYEKALQILRWNESTSKLQTTNG 402
Query: 72 PT---SEGSDMQLTKSEDSLESNCKSSIVKERRGCYKRRKISQTW--------EKESEQP 120
T SE + S E + +E + K+RK W E E P
Sbjct: 403 ATTLLSESPPLSANGSPMREEIDWAIQDHQEAKRDSKKRKTMPKWIDQIRVVSENGLEGP 582
Query: 121 EEDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKA 157
+DG+ WRKYGQK IL +PR+YYRCT +N Q C A
Sbjct: 583 HDDGYNWRKYGQKDILGAKYPRSYYRCTFRNTQNCWA 693
>TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcription
factor 49 (WRKY DNA-binding protein 49), partial (28%)
Length = 1009
Score = 67.0 bits (162), Expect = 3e-12
Identities = 36/87 (41%), Positives = 48/87 (54%), Gaps = 5/87 (5%)
Frame = +3
Query: 122 EDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH-- 179
+DG++WRKYGQK I ++ PR+YYRCT+ C A KQV+R EDP TY G H
Sbjct: 333 DDGYKWRKYGQKSIKNSPNPRSYYRCTNPR---CSAKKQVERSNEDPETLIITYEGLHLH 503
Query: 180 ---TCRILQNPEIIVDYPTDHSSMFLS 203
C ++ P+ +P S F S
Sbjct: 504 FAYPCFLVGQPQQSQSHPPVKKSKFTS 584
>TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding protein,
partial (45%)
Length = 517
Score = 67.0 bits (162), Expect = 3e-12
Identities = 30/58 (51%), Positives = 38/58 (64%)
Frame = +1
Query: 123 DGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHHT 180
DG++WRKYGQK + + FPR+YYRCTH GC K V R+ +D + TTY G HT
Sbjct: 1 DGYRWRKYGQKAVKNNKFPRSYYRCTH---HGCHVKKPVPRLTQDEGVVVTTYEGVHT 165
>TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcription
factor 53 (WRKY DNA-binding protein 53), partial (15%)
Length = 1044
Score = 65.1 bits (157), Expect = 1e-11
Identities = 26/46 (56%), Positives = 32/46 (69%)
Frame = +3
Query: 136 LHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHHTC 181
L FPR YYRCTH+N QGC+A KQVQ+ +DP ++ Y G HTC
Sbjct: 3 LGAKFPRGYYRCTHRNVQGCQATKQVQKSDKDPTMYEINYRGKHTC 140
>TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcription
factor 17 (WRKY DNA-binding protein 17), partial (53%)
Length = 1284
Score = 62.4 bits (150), Expect = 8e-11
Identities = 35/97 (36%), Positives = 51/97 (52%), Gaps = 1/97 (1%)
Frame = +3
Query: 84 SEDSLESNCKSSIVKERRGCYKRR-KISQTWEKESEQPEEDGHQWRKYGQKKILHTDFPR 142
S D SN K K R+ K+ ++ K ++ P ++ + WRKYGQK I T +PR
Sbjct: 624 SGDPSGSNTKCHCTKRRKNRVKKTIRVPAVSSKVADIPADE-YSWRKYGQKPIKGTPYPR 800
Query: 143 NYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
YY+C+ +GC A K V+R +DP + TY H
Sbjct: 801 GYYKCS--TVRGCPARKHVERATDDPAMLIVTYEDEH 905
>AV423663
Length = 447
Score = 62.4 bits (150), Expect = 8e-11
Identities = 32/77 (41%), Positives = 45/77 (57%), Gaps = 2/77 (2%)
Frame = +1
Query: 105 KRRK--ISQTWEKESEQPEEDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQ 162
KRRK + + +E D WRKYGQK I + +PR YYRC+ + +GC A KQV+
Sbjct: 22 KRRKNQFKKVCQVSAENLSSDIWAWRKYGQKPIKGSPYPRGYYRCS--SSKGCLARKQVE 195
Query: 163 RIQEDPPLHRTTYSGHH 179
R + +P + TY+G H
Sbjct: 196 RNKSEPAMFIVTYTGEH 246
>AV420160
Length = 417
Score = 61.2 bits (147), Expect = 2e-10
Identities = 39/103 (37%), Positives = 48/103 (45%), Gaps = 5/103 (4%)
Frame = +1
Query: 115 KESEQPEEDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTT 174
K P D WRKYGQK I + +PR YYRC+ + +GC A KQV+R + DP T
Sbjct: 133 KGETYPPSDSWAWRKYGQKPIKGSPYPRGYYRCS--SSKGCPARKQVERSRVDPTKLMVT 306
Query: 175 YSGHHTCRILQNPEIIVDYPTDHSS-----MFLSFDNSFPTPA 212
YS H + P HSS S D + TPA
Sbjct: 307 YSYEHNHSL--------PVPKSHSSAASATATTSSDGAADTPA 411
>TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (45%)
Length = 1257
Score = 60.5 bits (145), Expect = 3e-10
Identities = 27/57 (47%), Positives = 36/57 (62%)
Frame = +3
Query: 123 DGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
D + WRKYGQK I + +PR YY+C+ +GC A K V+R Q+DP + TY G H
Sbjct: 654 DEYSWRKYGQKPIKGSPYPRGYYKCS--TVRGCPARKHVERAQDDPNMLIVTYEGEH 818
>BP045724
Length = 490
Score = 60.1 bits (144), Expect = 4e-10
Identities = 48/102 (47%), Positives = 56/102 (54%), Gaps = 16/102 (15%)
Frame = -2
Query: 189 IIVD---YPTDHSSMFLSFDNSFPTPAKQDCPFL------SVKKEECKEEVVHPPP---- 235
IIVD P SSMF+SFDNS PT AKQ CPFL S K +CKEE P
Sbjct: 489 IIVDDSVCPNSDSSMFVSFDNSLPTSAKQQCPFLSSFPSPSSVKRKCKEEEEEEAPPSIS 310
Query: 236 SSNDYL---SGLTFDDPEKDVTLSSTLDSHDHLGVHISDIMY 274
SS+D+L S LTF D T DS D++GV I D++Y
Sbjct: 309 SSSDHLIPSSELTFHDS------PLTFDS-DYMGV-IPDVLY 208
>TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcription
factor 11 (WRKY DNA-binding protein 11), partial (27%)
Length = 579
Score = 59.3 bits (142), Expect = 7e-10
Identities = 30/87 (34%), Positives = 44/87 (50%)
Frame = +3
Query: 93 KSSIVKERRGCYKRRKISQTWEKESEQPEEDGHQWRKYGQKKILHTDFPRNYYRCTHKND 152
K K+R+ K+ ++ D + WRKYGQK I + +PR YY+C+
Sbjct: 3 KCHCTKKRKNRVKKTVRVPAISSKTADIPPDEYSWRKYGQKPIKGSPYPRGYYKCS--TV 176
Query: 153 QGCKAIKQVQRIQEDPPLHRTTYSGHH 179
+GC A K V+R +DP + TY G H
Sbjct: 177 RGCPARKHVERATDDPTMLIVTYEGEH 257
>TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%)
Length = 1389
Score = 58.5 bits (140), Expect = 1e-09
Identities = 27/58 (46%), Positives = 34/58 (58%)
Frame = +2
Query: 122 EDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
+DG++WRKYGQK + PR+YY+CTH GC K V+R D TTY G H
Sbjct: 404 DDGYRWRKYGQKVVKGNPNPRSYYKCTH---PGCPVRKHVERASHDLRAVITTYEGKH 568
>TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcription
factor 21 (WRKY DNA-binding protein 21), partial (22%)
Length = 590
Score = 57.4 bits (137), Expect = 3e-09
Identities = 26/57 (45%), Positives = 35/57 (60%)
Frame = +3
Query: 123 DGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
D + WRKYGQK I + PR YY+C+ + +GC A K V+R E+P + TY G H
Sbjct: 63 DDYSWRKYGQKPIKGSPHPRGYYKCS--SMRGCPARKHVERCLEEPTMLLVTYEGEH 227
>AV427157
Length = 379
Score = 55.8 bits (133), Expect = 8e-09
Identities = 26/58 (44%), Positives = 34/58 (57%)
Frame = +3
Query: 122 EDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
+DG++WRKYGQK + PR+YY+CT + GC K V+R D TTY G H
Sbjct: 207 DDGYRWRKYGQKVVKGNPNPRSYYKCT---NAGCMVRKHVERASHDLKSVITTYEGKH 371
>TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription factor 1,
partial (41%)
Length = 1717
Score = 54.7 bits (130), Expect = 2e-08
Identities = 47/171 (27%), Positives = 69/171 (39%), Gaps = 5/171 (2%)
Frame = +1
Query: 70 NNPTSEGSDMQLTKSEDSLESNCKSSIVKERRGCYKRRKISQTWEKESEQPEEDGHQWRK 129
NN SE S + EDS + + +I + Y R + + T +DG+QWRK
Sbjct: 454 NNGNSESS----STDEDSFKKPREETIKAKISRAYVRTEATDTGFVV-----KDGYQWRK 606
Query: 130 YGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHHTCRILQNPEI 189
YGQK PR Y++C+ C K+VQR +D + TY G H +P+
Sbjct: 607 YGQKVTRDNPCPRAYFKCSFA--PSCPVKKKVQRSVDDQSVLVATYEGEH-----NHPQP 765
Query: 190 IVDYPTDHSSMFLSFDNSFPTPAKQDCP-----FLSVKKEECKEEVVHPPP 235
T S +S S P+ P L + K C + + P
Sbjct: 766 SQMEATSGSGRNVSLVGSMPSSKSLSSPAPAVVTLDLTKSRCSNDSKNAEP 918
>TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcription
factor 15 (WRKY DNA-binding protein 15), partial (31%)
Length = 660
Score = 54.3 bits (129), Expect = 2e-08
Identities = 25/57 (43%), Positives = 34/57 (58%)
Frame = +3
Query: 123 DGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
D + WRKYGQK I + PR YY+C+ + +GC A K V+R +D + TY G H
Sbjct: 126 DDYSWRKYGQKPIKGSPHPRGYYKCS--SVRGCPARKHVERALDDAAMLVVTYEGEH 290
>TC11031 homologue to UP|WR65_ARATH (Q9LP56) Probable WRKY transcription
factor 65 (WRKY DNA-binding protein 65), partial (15%)
Length = 453
Score = 48.1 bits (113), Expect = 2e-06
Identities = 26/80 (32%), Positives = 39/80 (48%), Gaps = 8/80 (10%)
Frame = +3
Query: 86 DSLESNCKSSIVKERRGCYKRRKIS--------QTWEKESEQPEEDGHQWRKYGQKKILH 137
D L ++ +S K R ++R + + E+ P D WRKYGQK I
Sbjct: 216 DGLVTSSSTSSAKRSRRAIQKRVVQIPIKETEGSRLKAENNTPPSDSWAWRKYGQKPIKG 395
Query: 138 TDFPRNYYRCTHKNDQGCKA 157
+ +PR YYRC+ + +GC A
Sbjct: 396 SPYPRGYYRCS--SSKGCPA 449
>TC8298 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtWRKY3,
partial (16%)
Length = 514
Score = 40.0 bits (92), Expect = 4e-04
Identities = 17/38 (44%), Positives = 25/38 (65%)
Frame = +3
Query: 142 RNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 179
R YY+C+ +++GC A K V+R Q+DP + TY G H
Sbjct: 159 RGYYKCS--SEKGCPARKHVERAQDDPNMLIVTYEGEH 266
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,741,524
Number of Sequences: 28460
Number of extensions: 91655
Number of successful extensions: 403
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 388
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 388
length of query: 283
length of database: 4,897,600
effective HSP length: 89
effective length of query: 194
effective length of database: 2,364,660
effective search space: 458744040
effective search space used: 458744040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146784.5