BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146784.1 - phase: 0
(151 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16534 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcri... 47 2e-06
AV408330 39 5e-04
TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcri... 37 0.001
TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%) 37 0.001
BP046076 37 0.001
TC11031 homologue to UP|WR65_ARATH (Q9LP56) Probable WRKY transc... 37 0.002
TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding prot... 36 0.003
TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription fact... 35 0.004
TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcri... 34 0.009
TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-bi... 34 0.012
TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcrip... 32 0.036
AV427157 32 0.047
TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%) 32 0.047
AV420160 32 0.062
TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcrip... 30 0.14
TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcri... 29 0.31
AV774172 29 0.31
AV423663 29 0.40
TC19181 similar to UP|Q40090 (Q40090) SPF1 protein, partial (19%) 29 0.40
TC9312 similar to UP|TGA4_ARATH (Q39162) Transcription factor TG... 28 0.68
>TC16534 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcription
factor 41 (WRKY DNA-binding protein 41), partial (20%)
Length = 697
Score = 46.6 bits (109), Expect = 2e-06
Identities = 42/156 (26%), Positives = 68/156 (42%), Gaps = 24/156 (15%)
Frame = +1
Query: 15 VIDELRRGRELANQLKNILNESGDFDDINGSTTPFAEDL-LKEVLTTFTNSLLFLNNNPT 73
+I+EL +G E+A +LK +D+ + DL ++ +L+++ +L L N +
Sbjct: 223 LINELIQGMEVARRLK---------EDLKTPYSVNTRDLQVQRILSSYEKALQILRWNES 375
Query: 74 SEGSDMQLTKSEDSLESNCKSTSIV---------------KERRGCYKRRKVSQTW---- 114
+ S +Q T +L S S +E + K+RK W
Sbjct: 376 T--SKLQTTNGATTLLSESPPLSANGSPMREEIDWAIQDHQEAKRDSKKRKTMPKWIDQI 549
Query: 115 ----EKESDRPVEDGHQWRKYGQKKILHTDFPRYIY 146
E + P +DG+ WRKYGQK IL +PR Y
Sbjct: 550 RVVSENGLEGPHDDGYNWRKYGQKDILGAKYPRSYY 657
>AV408330
Length = 316
Score = 38.5 bits (88), Expect = 5e-04
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +3
Query: 122 VEDGHQWRKYGQKKILHTDFPRYIY 146
+EDG++WRKYGQK + ++ FPR Y
Sbjct: 126 LEDGYRWRKYGQKAVKNSPFPRSYY 200
>TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcription
factor 17 (WRKY DNA-binding protein 17), partial (53%)
Length = 1284
Score = 37.0 bits (84), Expect = 0.001
Identities = 22/63 (34%), Positives = 30/63 (46%)
Frame = +3
Query: 84 SEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDGHQWRKYGQKKILHTDFPR 143
S D SN K + + K +V K +D P ++ + WRKYGQK I T +PR
Sbjct: 624 SGDPSGSNTKCHCTKRRKNRVKKTIRVPAVSSKVADIPADE-YSWRKYGQKPIKGTPYPR 800
Query: 144 YIY 146
Y
Sbjct: 801 GYY 809
>TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%)
Length = 617
Score = 37.0 bits (84), Expect = 0.001
Identities = 24/69 (34%), Positives = 33/69 (47%)
Frame = +1
Query: 78 DMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDGHQWRKYGQKKIL 137
D LT S S C + K++ + +V K +D P +D QWRKYGQK I
Sbjct: 43 DATLTGKTSSSGSRCHCSK--KKKLRVKRTIRVPAISSKTADIPTDD-FQWRKYGQKPIK 213
Query: 138 HTDFPRYIY 146
+ +PR Y
Sbjct: 214 GSPYPRGYY 240
>BP046076
Length = 593
Score = 37.0 bits (84), Expect = 0.001
Identities = 26/75 (34%), Positives = 41/75 (54%), Gaps = 1/75 (1%)
Frame = -2
Query: 73 TSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPV-EDGHQWRKY 131
TS+G++ +S S +S K ++E R C++ R SD V +DG++WRKY
Sbjct: 580 TSDGNNTWW-RSGGSEKSKVKVRRKLREPRFCFQTR---------SDVDVLDDGYKWRKY 431
Query: 132 GQKKILHTDFPRYIY 146
GQK + ++ PR Y
Sbjct: 430 GQKVVKNSLHPRSYY 386
>TC11031 homologue to UP|WR65_ARATH (Q9LP56) Probable WRKY transcription
factor 65 (WRKY DNA-binding protein 65), partial (15%)
Length = 453
Score = 36.6 bits (83), Expect = 0.002
Identities = 23/68 (33%), Positives = 30/68 (43%), Gaps = 7/68 (10%)
Frame = +3
Query: 86 DSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDR-------PVEDGHQWRKYGQKKILH 138
D L ++ ++S + RR KR E E R P D WRKYGQK I
Sbjct: 216 DGLVTSSSTSSAKRSRRAIQKRVVQIPIKETEGSRLKAENNTPPSDSWAWRKYGQKPIKG 395
Query: 139 TDFPRYIY 146
+ +PR Y
Sbjct: 396 SPYPRGYY 419
>TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding protein,
partial (45%)
Length = 517
Score = 35.8 bits (81), Expect = 0.003
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +1
Query: 124 DGHQWRKYGQKKILHTDFPRYIY 146
DG++WRKYGQK + + FPR Y
Sbjct: 1 DGYRWRKYGQKAVKNNKFPRSYY 69
>TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription factor 1,
partial (41%)
Length = 1717
Score = 35.4 bits (80), Expect = 0.004
Identities = 28/81 (34%), Positives = 36/81 (43%)
Frame = +1
Query: 70 NNPTSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDGHQWR 129
NN SE S + EDS + + T K R Y R + + T V+DG+QWR
Sbjct: 454 NNGNSESS----STDEDSFKKPREETIKAKISRA-YVRTEATDT-----GFVVKDGYQWR 603
Query: 130 KYGQKKILHTDFPRYIYFYEF 150
KYGQK PR + F
Sbjct: 604 KYGQKVTRDNPCPRAYFKCSF 666
>TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcription
factor 49 (WRKY DNA-binding protein 49), partial (28%)
Length = 1009
Score = 34.3 bits (77), Expect = 0.009
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = +3
Query: 123 EDGHQWRKYGQKKILHTDFPRYIY 146
+DG++WRKYGQK I ++ PR Y
Sbjct: 333 DDGYKWRKYGQKSIKNSPNPRSYY 404
>TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (45%)
Length = 1257
Score = 33.9 bits (76), Expect = 0.012
Identities = 41/130 (31%), Positives = 56/130 (42%), Gaps = 25/130 (19%)
Frame = +3
Query: 42 INGSTTPFAEDLLKE----VLTTFTNSLLFLNNNPTSEGSDMQLTKSEDSLESNCKSTSI 97
I G T FA+ K+ + TT TNS F ++ T++GS + K L K
Sbjct: 342 IKGLTLDFAKPTNKDDTLTISTTNTNSSSF-KSSITADGS-VSDGKIGPFLTPAAKPPLS 515
Query: 98 VKERRGCY-------------KRRK--VSQTWE------KESDRPVEDGHQWRKYGQKKI 136
R+ C+ K+RK V QT K +D P D + WRKYGQK I
Sbjct: 516 TSHRKRCHDAAALSSGPCHCSKKRKSRVKQTIRVPAVSSKIADIP-PDEYSWRKYGQKPI 692
Query: 137 LHTDFPRYIY 146
+ +PR Y
Sbjct: 693 KGSPYPRGYY 722
>TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcription
factor 11 (WRKY DNA-binding protein 11), partial (27%)
Length = 579
Score = 32.3 bits (72), Expect = 0.036
Identities = 20/51 (39%), Positives = 25/51 (48%), Gaps = 8/51 (15%)
Frame = +3
Query: 104 CYKRRK--------VSQTWEKESDRPVEDGHQWRKYGQKKILHTDFPRYIY 146
C K+RK V K +D P D + WRKYGQK I + +PR Y
Sbjct: 12 CTKKRKNRVKKTVRVPAISSKTADIP-PDEYSWRKYGQKPIKGSPYPRGYY 161
>AV427157
Length = 379
Score = 32.0 bits (71), Expect = 0.047
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 122 VEDGHQWRKYGQKKILHTDFPRYIY 146
++DG++WRKYGQK + PR Y
Sbjct: 204 LDDGYRWRKYGQKVVKGNPNPRSYY 278
>TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%)
Length = 1389
Score = 32.0 bits (71), Expect = 0.047
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +2
Query: 122 VEDGHQWRKYGQKKILHTDFPRYIY 146
++DG++WRKYGQK + PR Y
Sbjct: 401 LDDGYRWRKYGQKVVKGNPNPRSYY 475
>AV420160
Length = 417
Score = 31.6 bits (70), Expect = 0.062
Identities = 20/54 (37%), Positives = 24/54 (44%), Gaps = 6/54 (11%)
Frame = +1
Query: 99 KERRGCYKR------RKVSQTWEKESDRPVEDGHQWRKYGQKKILHTDFPRYIY 146
K+RR KR V + K P D WRKYGQK I + +PR Y
Sbjct: 64 KKRREMKKRVVTLPIGDVDGSKSKGETYPPSDSWAWRKYGQKPIKGSPYPRGYY 225
>TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcription
factor 15 (WRKY DNA-binding protein 15), partial (31%)
Length = 660
Score = 30.4 bits (67), Expect = 0.14
Identities = 15/31 (48%), Positives = 19/31 (60%)
Frame = +3
Query: 116 KESDRPVEDGHQWRKYGQKKILHTDFPRYIY 146
K +D P +D + WRKYGQK I + PR Y
Sbjct: 105 KMADIPPDD-YSWRKYGQKPIKGSPHPRGYY 194
>TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcription
factor 21 (WRKY DNA-binding protein 21), partial (22%)
Length = 590
Score = 29.3 bits (64), Expect = 0.31
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +3
Query: 124 DGHQWRKYGQKKILHTDFPRYIY 146
D + WRKYGQK I + PR Y
Sbjct: 63 DDYSWRKYGQKPIKGSPHPRGYY 131
>AV774172
Length = 478
Score = 29.3 bits (64), Expect = 0.31
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +1
Query: 85 EDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDG 125
EDS S T K + C ++R VS+ EK S P++DG
Sbjct: 301 EDSKSSE*A*TLFTKSKH*CSEKRSVSE**EKNSTCPIKDG 423
>AV423663
Length = 447
Score = 28.9 bits (63), Expect = 0.40
Identities = 16/43 (37%), Positives = 22/43 (50%), Gaps = 2/43 (4%)
Frame = +1
Query: 106 KRRK--VSQTWEKESDRPVEDGHQWRKYGQKKILHTDFPRYIY 146
KRRK + + ++ D WRKYGQK I + +PR Y
Sbjct: 22 KRRKNQFKKVCQVSAENLSSDIWAWRKYGQKPIKGSPYPRGYY 150
>TC19181 similar to UP|Q40090 (Q40090) SPF1 protein, partial (19%)
Length = 402
Score = 28.9 bits (63), Expect = 0.40
Identities = 9/15 (60%), Positives = 14/15 (93%)
Frame = +3
Query: 122 VEDGHQWRKYGQKKI 136
++DG++WRKYGQK +
Sbjct: 342 LDDGYRWRKYGQKVV 386
>TC9312 similar to UP|TGA4_ARATH (Q39162) Transcription factor TGA4
(Ocs-element binding factor 4) (OBF4) (AtbZIP57),
partial (12%)
Length = 620
Score = 28.1 bits (61), Expect = 0.68
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = +2
Query: 20 RRGRELANQLKNILNESGDFDDINGSTT 47
+RGR LAN+ N +N G+ I G+TT
Sbjct: 116 QRGRTLANKPMNEVNAQGEIFKIKGNTT 199
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.132 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,473,656
Number of Sequences: 28460
Number of extensions: 28240
Number of successful extensions: 179
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of query: 151
length of database: 4,897,600
effective HSP length: 82
effective length of query: 69
effective length of database: 2,563,880
effective search space: 176907720
effective search space used: 176907720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146784.1