BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146776.7 + phase: 0
(271 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14209 similar to UP|AAQ72788 (AAQ72788) Hypersensitive-induced... 429 e-121
TC7840 similar to UP|AAQ72788 (AAQ72788) Hypersensitive-induced ... 422 e-119
TC17049 similar to UP|Q9FM19 (Q9FM19) Hypersensitive-induced res... 228 1e-60
TC9262 similar to UP|Q9FHM7 (Q9FHM7) Gb|AAF03497.1 (At5g51570), ... 165 7e-42
AV775598 120 3e-28
TC17023 homologue to UP|Q9FM19 (Q9FM19) Hypersensitive-induced r... 98 2e-21
TC14853 similar to UP|PZ12_LUPPO (P16148) PPLZ12 protein, partia... 91 2e-19
TC7841 homologue to UP|1433_OENHO (P29307) 14-3-3-like protein, ... 44 3e-05
TC14757 similar to UP|O04361 (O04361) Prohibitin, complete 31 0.20
TC13946 homologue to UP|MSI4_ARATH (O22607) WD-40 repeat protein... 26 8.2
TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 26 8.2
BP034123 26 8.2
>TC14209 similar to UP|AAQ72788 (AAQ72788) Hypersensitive-induced response
protein, complete
Length = 1298
Score = 429 bits (1102), Expect = e-121
Identities = 221/282 (78%), Positives = 245/282 (86%), Gaps = 13/282 (4%)
Frame = +1
Query: 1 MGNIVCCVQVDQSQVAMKEGFGKFEKVLHPGCHCMPWFLGKRIAGHLSLR---------- 50
MG + CVQVDQS VA+KE FGKF ++L PGCHC+PW LG +IAG LSLR
Sbjct: 64 MGQALGCVQVDQSNVAIKEHFGKFSEILEPGCHCLPWCLGYQIAGGLSLRVQQLDVRCET 243
Query: 51 ---DNVFVNVVASIQYRALANKANDAFYKLSNTRGQIQAYVFDVIRASVPKLNLDDTFEQ 107
DNVFV VVAS+QYRA+A+KA+DAFY+L+NTR QIQ+YVFDVIRASVPKL LD FEQ
Sbjct: 244 KTKDNVFVTVVASVQYRAVADKASDAFYRLTNTREQIQSYVFDVIRASVPKLELDSVFEQ 423
Query: 108 KNEIAKAVEEEREKAMSAYGYEIVQTLITDIEPDQHVKTAMNEINAAARMRIAANEKAEA 167
KN+IAK+VEEE EKAMS YGYEIVQTLI DIEPD +VK AMNEINAAARMR+AANEKAEA
Sbjct: 424 KNDIAKSVEEELEKAMSTYGYEIVQTLIVDIEPDVNVKRAMNEINAAARMRLAANEKAEA 603
Query: 168 EKILQIKRAEGEAESKYLSGMGIARQRQAIVDGLRDSVIGFSENVPGPSAKDVMDMVLVT 227
EKILQIK+AEGEAESKYLSG+GIARQRQAIVDGLRDSV+ FSENVPG SAKDVMDMVLVT
Sbjct: 604 EKILQIKKAEGEAESKYLSGLGIARQRQAIVDGLRDSVLAFSENVPGTSAKDVMDMVLVT 783
Query: 228 QYFDTMKEIGAASKSSAVFIPHGPGAVRDVASQICDGLLQGS 269
QYFDTMKEIGA+SKSS+VFIPHGPGAVRD+A QI DGLLQG+
Sbjct: 784 QYFDTMKEIGASSKSSSVFIPHGPGAVRDIAVQIRDGLLQGN 909
>TC7840 similar to UP|AAQ72788 (AAQ72788) Hypersensitive-induced response
protein, complete
Length = 1585
Score = 422 bits (1084), Expect = e-119
Identities = 215/282 (76%), Positives = 243/282 (85%), Gaps = 13/282 (4%)
Frame = +1
Query: 1 MGNIVCCVQVDQSQVAMKEGFGKFEKVLHPGCHCMPWFLGKRIAGHLSLR---------- 50
MG + CVQVDQS VA+KE FGK++ VL PGCHC+PW +G +I+G LSLR
Sbjct: 244 MGQTLGCVQVDQSSVAIKEVFGKYDDVLQPGCHCVPWCIGSQISGSLSLRVKQLDVRCET 423
Query: 51 ---DNVFVNVVASIQYRALANKANDAFYKLSNTRGQIQAYVFDVIRASVPKLNLDDTFEQ 107
DNVFV VVASIQYRALA+KA DA+YKLS+T+ QIQAYVFDVIRASVPK+ LD FEQ
Sbjct: 424 KTKDNVFVTVVASIQYRALADKAVDAYYKLSDTKAQIQAYVFDVIRASVPKMELDSAFEQ 603
Query: 108 KNEIAKAVEEEREKAMSAYGYEIVQTLITDIEPDQHVKTAMNEINAAARMRIAANEKAEA 167
KNEIAKAVEEE EKAMSAYGYEIVQTLI DIEPD+ VK AMNEINAAAR+R+A EKAEA
Sbjct: 604 KNEIAKAVEEELEKAMSAYGYEIVQTLIVDIEPDERVKKAMNEINAAARLRVATKEKAEA 783
Query: 168 EKILQIKRAEGEAESKYLSGMGIARQRQAIVDGLRDSVIGFSENVPGPSAKDVMDMVLVT 227
EKILQIKRAEG+AESKYL+G+GIARQRQAIVDGLRDSV+ FSENVPG S+KD+MDMVLVT
Sbjct: 784 EKILQIKRAEGDAESKYLAGLGIARQRQAIVDGLRDSVLAFSENVPGTSSKDIMDMVLVT 963
Query: 228 QYFDTMKEIGAASKSSAVFIPHGPGAVRDVASQICDGLLQGS 269
QYFDTMKEIGA++KS+AVFIPHGPGAV+D+ SQI DGLLQG+
Sbjct: 964 QYFDTMKEIGASAKSNAVFIPHGPGAVKDITSQIRDGLLQGN 1089
>TC17049 similar to UP|Q9FM19 (Q9FM19) Hypersensitive-induced response
protein, partial (53%)
Length = 602
Score = 228 bits (580), Expect = 1e-60
Identities = 114/152 (75%), Positives = 127/152 (83%), Gaps = 13/152 (8%)
Frame = +3
Query: 1 MGNIVCCVQVDQSQVAMKEGFGKFEKVLHPGCHCMPWFLGKRIAGHLSLR---------- 50
MGN+ CCVQVDQS VA+KE FG+FE+VL PGCHC+PW LG ++AGHLS+R
Sbjct: 147 MGNLFCCVQVDQSTVAIKERFGRFEEVLQPGCHCLPWILGSQLAGHLSIRVQQLDVKCET 326
Query: 51 ---DNVFVNVVASIQYRALANKANDAFYKLSNTRGQIQAYVFDVIRASVPKLNLDDTFEQ 107
DNVFVNVVAS+QYRAL+ KA+DAFYKLSNT+ QIQAYVFDVIRASVPKL LDD FEQ
Sbjct: 327 KTKDNVFVNVVASVQYRALSEKASDAFYKLSNTKTQIQAYVFDVIRASVPKLLLDDAFEQ 506
Query: 108 KNEIAKAVEEEREKAMSAYGYEIVQTLITDIE 139
KN+IAKAVEEE EKAMSAYGYEIVQTLI DIE
Sbjct: 507 KNDIAKAVEEELEKAMSAYGYEIVQTLIVDIE 602
>TC9262 similar to UP|Q9FHM7 (Q9FHM7) Gb|AAF03497.1 (At5g51570), partial
(63%)
Length = 584
Score = 165 bits (418), Expect = 7e-42
Identities = 92/185 (49%), Positives = 115/185 (61%), Gaps = 15/185 (8%)
Frame = +1
Query: 1 MGNIVC--CVQVDQSQVAMKEGFGKFEKVLHPGCHCMPWFLGKRIAGHLSLR-------- 50
MGN C C + Q+ V + E +G+F +V PG H G+ +AG LS R
Sbjct: 28 MGNTFCFFCGCIGQASVGVVEHWGRFHRVAQPGFHFFNPLAGECLAGVLSTRIASLDVRI 207
Query: 51 -----DNVFVNVVASIQYRALANKANDAFYKLSNTRGQIQAYVFDVIRASVPKLNLDDTF 105
DNVFV ++ SIQYR + A+DAFY+L N + QIQAYVFDV RA VP++NLDD F
Sbjct: 208 ETKTKDNVFVQLLCSIQYRVVKENADDAFYELQNPQEQIQAYVFDVTRAIVPRMNLDDLF 387
Query: 106 EQKNEIAKAVEEEREKAMSAYGYEIVQTLITDIEPDQHVKTAMNEINAAARMRIAANEKA 165
EQK E+AKAV EE K M YGY I L+ DI PD V+ AMNEINAA RM +A+ K
Sbjct: 388 EQKGEVAKAVLEELHKVMGEYGYSIEHILMVDIIPDPSVRKAMNEINAAQRMLLASEFKG 567
Query: 166 EAEKI 170
+AEK+
Sbjct: 568 DAEKV 582
>AV775598
Length = 448
Score = 120 bits (300), Expect = 3e-28
Identities = 61/79 (77%), Positives = 67/79 (84%)
Frame = +3
Query: 63 YRALANKANDAFYKLSNTRGQIQAYVFDVIRASVPKLNLDDTFEQKNEIAKAVEEEREKA 122
YRAL +KA DA+YKLS+T+ QIQAYVFDVIRA PK+ LD EQKNE+A AVEEE EKA
Sbjct: 210 YRALTDKAVDAYYKLSDTKTQIQAYVFDVIRAQCPKMELDSAPEQKNEMATAVEEELEKA 389
Query: 123 MSAYGYEIVQTLITDIEPD 141
MSAYGYEIVQTLI DIEPD
Sbjct: 390 MSAYGYEIVQTLIVDIEPD 446
>TC17023 homologue to UP|Q9FM19 (Q9FM19) Hypersensitive-induced response
protein, partial (29%)
Length = 510
Score = 97.8 bits (242), Expect = 2e-21
Identities = 50/65 (76%), Positives = 55/65 (83%)
Frame = +3
Query: 187 GMGIARQRQAIVDGLRDSVIGFSENVPGPSAKDVMDMVLVTQYFDTMKEIGAASKSSAVF 246
G+GIARQRQAIVDGLRDSV+GFS NVPG +AKDV+DMVLVTQYFDTMKEIGA S+ F
Sbjct: 6 GVGIARQRQAIVDGLRDSVLGFSGNVPGTTAKDVLDMVLVTQYFDTMKEIGATSQVFCCF 185
Query: 247 IPHGP 251
H P
Sbjct: 186 --HSP 194
Score = 56.6 bits (135), Expect = 4e-09
Identities = 28/37 (75%), Positives = 30/37 (80%)
Frame = +1
Query: 234 KEIGAASKSSAVFIPHGPGAVRDVASQICDGLLQGSH 270
K++ KSSAVFIPHGPGAVRDVAS I DGLLQ SH
Sbjct: 148 KKLVLPPKSSAVFIPHGPGAVRDVASPIRDGLLQASH 258
>TC14853 similar to UP|PZ12_LUPPO (P16148) PPLZ12 protein, partial (40%)
Length = 531
Score = 91.3 bits (225), Expect = 2e-19
Identities = 37/71 (52%), Positives = 59/71 (82%)
Frame = +3
Query: 199 DGLRDSVIGFSENVPGPSAKDVMDMVLVTQYFDTMKEIGAASKSSAVFIPHGPGAVRDVA 258
DGLR++++ FS + G SAK+VMD+++VTQYFDT+K++G +SK++ +FIPHGPG VRD+
Sbjct: 3 DGLRENILNFSGKLEGTSAKEVMDLIMVTQYFDTIKDLGNSSKNTTIFIPHGPGHVRDIG 182
Query: 259 SQICDGLLQGS 269
QI +G+++ +
Sbjct: 183 DQIRNGVMEAA 215
>TC7841 homologue to UP|1433_OENHO (P29307) 14-3-3-like protein, partial
(12%)
Length = 446
Score = 43.9 bits (102), Expect = 3e-05
Identities = 18/27 (66%), Positives = 22/27 (80%)
Frame = -1
Query: 10 VDQSQVAMKEGFGKFEKVLHPGCHCMP 36
VDQS VA+KE GK++ VL PGCHC+P
Sbjct: 83 VDQSSVAIKEVNGKYDDVLQPGCHCVP 3
>TC14757 similar to UP|O04361 (O04361) Prohibitin, complete
Length = 1141
Score = 31.2 bits (69), Expect = 0.20
Identities = 27/115 (23%), Positives = 48/115 (41%), Gaps = 10/115 (8%)
Frame = +3
Query: 90 DVIRASVPKLNLDDTFEQKNEIAKAVEEEREKAMSAYGYEIVQTLITDIEPDQHVKTAMN 149
+V++A V + N D ++ +++ V E + + + IT + A+
Sbjct: 423 EVLKAVVAQFNADQLLTERPQVSALVRESLLRRAKDFNILLDDVAITHLSYGAEFSRAVE 602
Query: 150 EINAAARMRIAAN---EKAEAEKILQIKRAEGEAESKYL-------SGMGIARQR 194
+ A + + KAE E+ I RAEGE+E+ L GMG+ R
Sbjct: 603 QKQVAQQEAERSKFVVMKAEQERRAAIIRAEGESEAAKLISDATAAVGMGLIELR 767
>TC13946 homologue to UP|MSI4_ARATH (O22607) WD-40 repeat protein MSI4,
partial (15%)
Length = 573
Score = 25.8 bits (55), Expect = 8.2
Identities = 17/46 (36%), Positives = 23/46 (49%), Gaps = 1/46 (2%)
Frame = -1
Query: 41 KRIAGHLSLRDNVFVNVVASIQYRALAN-KANDAFYKLSNTRGQIQ 85
KR+ G + LRD A+IQY L+N F KL N + I+
Sbjct: 462 KRVNGIIILRDINLRKFYAAIQYSQLSNIPTRGWFKKLRNIQSMIE 325
>TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (74%)
Length = 778
Score = 25.8 bits (55), Expect = 8.2
Identities = 19/75 (25%), Positives = 27/75 (35%)
Frame = -1
Query: 190 IARQRQAIVDGLRDSVIGFSENVPGPSAKDVMDMVLVTQYFDTMKEIGAASKSSAVFIPH 249
+A + V GL + E PG + V + + AA+ + A PH
Sbjct: 319 LAIEPTGAVSGLAPAAASTVEVAPGAAPDTGHCSACVDPQVPCLAAVEAAASTRAGSCPH 140
Query: 250 GPGAVRDVASQICDG 264
GAV D C G
Sbjct: 139 SVGAVVDDLKVCCSG 95
>BP034123
Length = 548
Score = 25.8 bits (55), Expect = 8.2
Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = +1
Query: 155 ARMRIAANEKAEAEKILQIKRAEGEAESKYLSGMGIARQRQAIVDGLRDSVIGFSENVP- 213
AR + + E+ EA K +IKR + E E +G+A +R G R FSE V
Sbjct: 205 ARDKKSQTEEMEAAKE-EIKRIKEEEEQAMREALGLAPKRANRPQGNRLDKHEFSELVKR 381
Query: 214 GPSAKDV 220
G +A+D+
Sbjct: 382 GSTAEDL 402
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.133 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,386,116
Number of Sequences: 28460
Number of extensions: 33820
Number of successful extensions: 184
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of query: 271
length of database: 4,897,600
effective HSP length: 89
effective length of query: 182
effective length of database: 2,364,660
effective search space: 430368120
effective search space used: 430368120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146776.7