BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146776.15 + phase: 0
(536 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI420856 123 7e-29
TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%) 111 3e-25
TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription fact... 110 7e-25
AV408330 90 8e-19
AV427157 89 2e-18
BP046076 87 7e-18
AV420160 82 2e-16
TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcri... 80 8e-16
TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcrip... 80 8e-16
TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%) 80 1e-15
TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcrip... 78 3e-15
TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcri... 76 1e-14
TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-bi... 76 2e-14
AV423663 75 3e-14
TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcri... 74 6e-14
TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding prot... 72 3e-13
TC15932 similar to UP|WR61_ARATH (Q8VWV6) Probable WRKY transcri... 71 5e-13
TC15166 similar to GB|AAK28312.1|13506739|AF224702 WRKY DNA-bind... 59 3e-09
AU240092 57 6e-09
TC8298 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtWRK... 53 1e-08
>BI420856
Length = 354
Score = 123 bits (309), Expect = 7e-29
Identities = 75/114 (65%), Positives = 87/114 (75%), Gaps = 4/114 (3%)
Frame = +2
Query: 1 MGEVREENQRLKMCLNKIMTEYRALEMQFNNMVKQ-ETKKN---NDNNHEEMNAESDLVS 56
MGEVREENQRLKM LNKIM EYR +QF ++ KQ +TKKN +DNNH+E+ ESDLVS
Sbjct: 35 MGEVREENQRLKMSLNKIMNEYRIFVLQFQDIAKQDQTKKNIVADDNNHQEIMEESDLVS 214
Query: 57 LSLGRVPSNNIPKNDQEKVNKVSKLALNNDEEFNKEELSLGLECKFETSKSGST 110
LSLGRVPSN P+N KVSK L + EEFN E+L+LGL+CK ETSKSGST
Sbjct: 215 LSLGRVPSN--PRN-----IKVSK-PLKDHEEFN-EDLTLGLDCKLETSKSGST 349
>TC14849 similar to UP|Q40090 (Q40090) SPF1 protein, partial (37%)
Length = 1389
Score = 111 bits (277), Expect = 3e-25
Identities = 77/231 (33%), Positives = 104/231 (44%), Gaps = 1/231 (0%)
Frame = +2
Query: 123 SSEVVPIKNDEVVETWPPSKTLNKTMRDAEDEVAQQTPAKKARVCVRARCDTPTMNDGCQ 182
SS+ DE+ E P SK + ++ RV V+ D ++DG +
Sbjct: 239 SSQKCRSGGDELDEDEPDSKRWKVEGENEGISAPGSRTVREPRVVVQTTSDIDILDDGYR 418
Query: 183 WRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSLPLSAT 242
WRKYGQK+ KGNP PR+YY+CT P CPVRK V+R D+ +ITTYEG HNH +P
Sbjct: 419 WRKYGQKVVKGNPNPRSYYKCT-HPGCPVRKHVERASHDLRAVITTYEGKHNHDVP---- 583
Query: 243 AMASTTSAAASMLLSGSSTSNSGSMPSAQTNNNLHGLNFYLPDGTKSNQLYLSNPALSSQ 302
A+ S S+ + + + PS+ T+ + H N S Q A
Sbjct: 584 --AARGSGNHSVTRPLPNNTANAIRPSSVTHQHHHNTN------NNSLQSLRPQQAPDQV 739
Query: 303 HSHPTITLDLTSNPSNSSTSSPFVRFNSSYN-NNNQLPRYPSSTLSFSSPE 352
S P L N SS F SYN NN Q + P + S + E
Sbjct: 740 QSSPFTLEMLQQNQGGFGFSSGFGNSMGSYNMNNQQQQQLPDNVFSSRAKE 892
Score = 26.9 bits (58), Expect = 8.3
Identities = 21/65 (32%), Positives = 28/65 (42%), Gaps = 10/65 (15%)
Frame = +2
Query: 221 DMSILITTYEGTHNHSLPLSATAMASTTSAAASMLLSGS----------STSNSGSMPSA 270
D I Y+GTHNH P AT S++S++ + S +T SG M S
Sbjct: 11 DGQITEIVYKGTHNHPKP-QATRRNSSSSSSLPIPHSNHISNDIQDQSYATHGSGQMDSV 187
Query: 271 QTNNN 275
T N
Sbjct: 188 ATPEN 202
>TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription factor 1,
partial (41%)
Length = 1717
Score = 110 bits (274), Expect = 7e-25
Identities = 56/114 (49%), Positives = 73/114 (63%), Gaps = 2/114 (1%)
Frame = +1
Query: 161 AKKARVCVRARC-DTP-TMNDGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRC 218
AK +R VR DT + DG QWRKYGQK+ + NPCPRAY++C+ APSCPV+K+VQR
Sbjct: 526 AKISRAYVRTEATDTGFVVKDGYQWRKYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRS 705
Query: 219 VEDMSILITTYEGTHNHSLPLSATAMASTTSAAASMLLSGSSTSNSGSMPSAQT 272
V+D S+L+ TYEG HNH P A + SG + S GSMPS+++
Sbjct: 706 VDDQSVLVATYEGEHNHPQPSQMEATSG----------SGRNVSLVGSMPSSKS 837
>AV408330
Length = 316
Score = 90.1 bits (222), Expect = 8e-19
Identities = 42/95 (44%), Positives = 59/95 (61%)
Frame = +3
Query: 145 NKTMRDAEDEVAQQTPAKKARVCVRARCDTPTMNDGCQWRKYGQKIAKGNPCPRAYYRCT 204
NKT + + + Q ++ R + + + DG +WRKYGQK K +P PR+YYRCT
Sbjct: 30 NKTKKQLKAKKTNQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCT 209
Query: 205 VAPSCPVRKQVQRCVEDMSILITTYEGTHNHSLPL 239
A SC V+K+V+R D SI++TTYEG H HS P+
Sbjct: 210 SA-SCNVKKRVERSFTDPSIVVTTYEGQHTHSSPV 311
>AV427157
Length = 379
Score = 89.0 bits (219), Expect = 2e-18
Identities = 43/96 (44%), Positives = 62/96 (63%), Gaps = 1/96 (1%)
Frame = +3
Query: 141 SKTLNKTMRDAEDEVAQQTPA-KKARVCVRARCDTPTMNDGCQWRKYGQKIAKGNPCPRA 199
S++ + + E++ T A ++ RV V+ + ++DG +WRKYGQK+ KGNP PR+
Sbjct: 93 SESKRRKLESYATELSGATRAIREPRVVVQTTSEVDILDDGYRWRKYGQKVVKGNPNPRS 272
Query: 200 YYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNH 235
YY+CT A C VRK V+R D+ +ITTYEG HNH
Sbjct: 273 YYKCTNA-GCMVRKHVERASHDLKSVITTYEGKHNH 377
>BP046076
Length = 593
Score = 87.0 bits (214), Expect = 7e-18
Identities = 39/85 (45%), Positives = 58/85 (67%)
Frame = -2
Query: 152 EDEVAQQTPAKKARVCVRARCDTPTMNDGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPV 211
+ +V + ++ R C + R D ++DG +WRKYGQK+ K + PR+YYRCT + +C V
Sbjct: 535 KSKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHS-NCRV 359
Query: 212 RKQVQRCVEDMSILITTYEGTHNHS 236
+K+V+R ED ++ITTYEG HNHS
Sbjct: 358 KKRVERLSEDCRMVITTYEGRHNHS 284
>AV420160
Length = 417
Score = 82.4 bits (202), Expect = 2e-16
Identities = 41/91 (45%), Positives = 58/91 (63%), Gaps = 6/91 (6%)
Frame = +1
Query: 169 RARCDTPTMNDGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITT 228
+++ +T +D WRKYGQK KG+P PR YYRC+ + CP RKQV+R D + L+ T
Sbjct: 127 KSKGETYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTKLMVT 306
Query: 229 YEGTHNHSLPL------SATAMASTTSAAAS 253
Y HNHSLP+ +A+A A+T+S A+
Sbjct: 307 YSYEHNHSLPVPKSHSSAASATATTSSDGAA 399
>TC14909 similar to UP|WR21_ARATH (O04336) Probable WRKY transcription
factor 21 (WRKY DNA-binding protein 21), partial (22%)
Length = 590
Score = 80.1 bits (196), Expect = 8e-16
Identities = 32/57 (56%), Positives = 41/57 (71%)
Frame = +3
Query: 179 DGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNH 235
D WRKYGQK KG+P PR YY+C+ CP RK V+RC+E+ ++L+ TYEG HNH
Sbjct: 63 DDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPTMLLVTYEGEHNH 233
>TC9067 similar to UP|WR15_ARATH (O22176) Probable WRKY transcription
factor 15 (WRKY DNA-binding protein 15), partial (31%)
Length = 660
Score = 80.1 bits (196), Expect = 8e-16
Identities = 32/59 (54%), Positives = 42/59 (70%)
Frame = +3
Query: 179 DGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSL 237
D WRKYGQK KG+P PR YY+C+ CP RK V+R ++D ++L+ TYEG HNH+L
Sbjct: 126 DDYSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERALDDAAMLVVTYEGEHNHAL 302
>TC8297 similar to UP|Q40828 (Q40828) WRKY3, partial (27%)
Length = 617
Score = 79.7 bits (195), Expect = 1e-15
Identities = 36/69 (52%), Positives = 45/69 (65%)
Frame = +1
Query: 173 DTPTMNDGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGT 232
D PT D QWRKYGQK KG+P PR YY+C+ CP RK V+R +D ++LI TYEG
Sbjct: 160 DIPT--DDFQWRKYGQKPIKGSPYPRGYYKCSSEKGCPARKHVERAQDDPNMLIVTYEGE 333
Query: 233 HNHSLPLSA 241
H H + +A
Sbjct: 334 HRHLITTTA 360
>TC7955 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcription
factor 11 (WRKY DNA-binding protein 11), partial (27%)
Length = 579
Score = 78.2 bits (191), Expect = 3e-15
Identities = 32/59 (54%), Positives = 40/59 (67%)
Frame = +3
Query: 179 DGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSL 237
D WRKYGQK KG+P PR YY+C+ CP RK V+R +D ++LI TYEG H H+L
Sbjct: 93 DEYSWRKYGQKPIKGSPYPRGYYKCSTVRGCPARKHVERATDDPTMLIVTYEGEHRHAL 269
>TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcription
factor 17 (WRKY DNA-binding protein 17), partial (53%)
Length = 1284
Score = 76.3 bits (186), Expect = 1e-14
Identities = 35/77 (45%), Positives = 44/77 (56%)
Frame = +3
Query: 179 DGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSLP 238
D WRKYGQK KG P PR YY+C+ CP RK V+R +D ++LI TYE H+H +
Sbjct: 741 DEYSWRKYGQKPIKGTPYPRGYYKCSTVRGCPARKHVERATDDPAMLIVTYEDEHDHGI- 917
Query: 239 LSATAMASTTSAAASML 255
TAM S A +
Sbjct: 918 --QTAMHDNISGAVGFV 962
>TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (45%)
Length = 1257
Score = 75.9 bits (185), Expect = 2e-14
Identities = 32/67 (47%), Positives = 41/67 (60%)
Frame = +3
Query: 179 DGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSLP 238
D WRKYGQK KG+P PR YY+C+ CP RK V+R +D ++LI TYEG H H
Sbjct: 654 DEYSWRKYGQKPIKGSPYPRGYYKCSTVRGCPARKHVERAQDDPNMLIVTYEGEHRHQQQ 833
Query: 239 LSATAMA 245
+A +
Sbjct: 834 QTAVGFS 854
>AV423663
Length = 447
Score = 74.7 bits (182), Expect = 3e-14
Identities = 32/66 (48%), Positives = 42/66 (63%)
Frame = +1
Query: 183 WRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSLPLSAT 242
WRKYGQK KG+P PR YYRC+ + C RKQV+R + ++ I TY G HNH P
Sbjct: 94 WRKYGQKPIKGSPYPRGYYRCSSSKGCLARKQVERNKSEPAMFIVTYTGEHNHPAPTHRN 273
Query: 243 AMASTT 248
++A +T
Sbjct: 274 SLAGST 291
>TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcription
factor 49 (WRKY DNA-binding protein 49), partial (28%)
Length = 1009
Score = 73.9 bits (180), Expect = 6e-14
Identities = 58/184 (31%), Positives = 85/184 (45%), Gaps = 1/184 (0%)
Frame = +3
Query: 178 NDGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNH-S 236
+DG +WRKYGQK K +P PR+YYRCT P C +KQV+R ED LI TYEG H H +
Sbjct: 333 DDGYKWRKYGQKSIKNSPNPRSYYRCT-NPRCSAKKQVERSNEDPETLIITYEGLHLHFA 509
Query: 237 LPLSATAMASTTSAAASMLLSGSSTSNSGSMPSAQTNNNLHGLNFYLPDGTKSNQLYLSN 296
P + + + S ++S + AQ +F + + S + L++
Sbjct: 510 YPCFLVGQPQQSQSHPPVKKSKFTSSQVQAQAQAQ--------DFIVNEAQASASVGLTS 665
Query: 297 PALSSQHSHPTITLDLTSNPSNSSTSSPFVRFNSSYNNNNQLPRYPSSTLSFSSPESNPM 356
P L +S + + PF+ N S N N+ + S + SP S P+
Sbjct: 666 PTL--LNSPQDMAQQNLGSQGLLEDMVPFMVRNPSNNLNSGYSKLSCS--ADPSPTSPPL 833
Query: 357 HWNS 360
W S
Sbjct: 834 -WTS 842
>TC11124 similar to UP|Q8H9D7 (Q8H9D7) WRKY-type DNA binding protein,
partial (45%)
Length = 517
Score = 71.6 bits (174), Expect = 3e-13
Identities = 31/59 (52%), Positives = 39/59 (65%)
Frame = +1
Query: 179 DGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSL 237
DG +WRKYGQK K N PR+YYRCT C V+K V R +D +++TTYEG H H +
Sbjct: 1 DGYRWRKYGQKAVKNNKFPRSYYRCT-HHGCHVKKPVPRLTQDEGVVVTTYEGVHTHPI 174
>TC15932 similar to UP|WR61_ARATH (Q8VWV6) Probable WRKY transcription
factor 61 (WRKY DNA-binding protein 61), partial (6%)
Length = 454
Score = 70.9 bits (172), Expect = 5e-13
Identities = 49/97 (50%), Positives = 58/97 (59%), Gaps = 5/97 (5%)
Frame = -2
Query: 231 GTHNHSLPLSATAMASTTSAAASMLLSGSSTSN-----SGSMPSAQTNNNLHGLNFYLPD 285
GTHNH LP+SATAM S+TSAA SML SGS TS + SM SA T GLNF +
Sbjct: 336 GTHNHPLPVSATAMDSSTSAALSML*SGSYTSQPTGHCAASMGSAST--MFIGLNFSSYN 163
Query: 286 GTKSNQLYLSNPALSSQHSHPTITLDLTSNPSNSSTS 322
+++ Q +L P + H PTITLDLT S S S
Sbjct: 162 QSRATQAFLPTP---TPHLFPTITLDLT*TTSESWNS 61
>TC15166 similar to GB|AAK28312.1|13506739|AF224702 WRKY DNA-binding protein
6 {Arabidopsis thaliana;} , partial (26%)
Length = 668
Score = 58.5 bits (140), Expect = 3e-09
Identities = 54/169 (31%), Positives = 75/169 (43%), Gaps = 11/169 (6%)
Frame = +3
Query: 306 PTITLDLTSNPSNSSTSSPFVRFNSSYNNNNQLPR-YPSSTLSFSSPESNPMHWNSFLNY 364
PT+TLDLT NP+ S P ++ Q+P+ + S SF+ ++ P++
Sbjct: 15 PTVTLDLTHNPNPLQFSRP------QHSAPFQIPQNFMSGPASFA--QAAPLY------- 149
Query: 365 ATTQNQPYSNNRNNNNLSTLNFGRQNTMESIYQTYMQKNNNSSNISQHVGLQDSTISAAT 424
N + S L Q + S +Q Q+ Q L D T+SAAT
Sbjct: 150 ------------NQSKFSGLQLSSQQEVGSSHQLASQQPPQ-----QQPSLAD-TVSAAT 275
Query: 425 KAITADPTFQSALAAALSSLIG----------NTTNQGNQNQSAGENLS 463
AITADP F + LAAA+SS+IG N N N N+S N S
Sbjct: 276 AAITADPNFTAVLAAAISSIIGGSHANNNGSHNNNNNNNNNKSTMSNFS 422
>AU240092
Length = 300
Score = 57.4 bits (137), Expect = 6e-09
Identities = 42/91 (46%), Positives = 50/91 (54%), Gaps = 2/91 (2%)
Frame = +2
Query: 233 HNHSLPLSATAMASTTSAAASMLLSGSSTSNSGSMPSAQTNNNLHGL--NFYLPDGTKSN 290
H+H LP +A AMASTTSAAASMLL SGSMPSA HGL L + +
Sbjct: 5 HSHPLPPTAQAMASTTSAAASMLL-------SGSMPSAD-----HGLINPTILETASLHS 148
Query: 291 QLYLSNPALSSQHSHPTITLDLTSNPSNSST 321
+ LS+ PTITLDLT S +S+
Sbjct: 149 SAQQNMATLSASAPFPTITLDLTQTDSTNSS 241
>TC8298 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtWRKY3,
partial (16%)
Length = 514
Score = 52.8 bits (125), Expect(2) = 1e-08
Identities = 21/46 (45%), Positives = 29/46 (62%)
Frame = +3
Query: 196 CPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSLPLSA 241
C R YY+C+ CP RK V+R +D ++LI TYEG H H + +A
Sbjct: 153 CCRGYYKCSSEKGCPARKHVERAQDDPNMLIVTYEGEHRHLITTTA 290
Score = 23.1 bits (48), Expect(2) = 1e-08
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +1
Query: 186 YGQKIAKGNPCPR 198
YGQK KG+P PR
Sbjct: 1 YGQKPIKGSPYPR 39
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.306 0.121 0.337
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,877,463
Number of Sequences: 28460
Number of extensions: 129309
Number of successful extensions: 1030
Number of sequences better than 10.0: 118
Number of HSP's better than 10.0 without gapping: 962
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1003
length of query: 536
length of database: 4,897,600
effective HSP length: 95
effective length of query: 441
effective length of database: 2,193,900
effective search space: 967509900
effective search space used: 967509900
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146776.15