Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146759.1 + phase: 0 /pseudo
         (188 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BP053584                                                               28  1.3
TC9442 similar to GB|BAC55960.1|28071265|AB087408 actin binding ...    27  2.9
TC8427 homologue to UP|Q84L15 (Q84L15) Cytosolic glutamine synth...    27  2.9
TC19952                                                                26  3.7
TC9573                                                                 25  6.4
TC17084                                                                25  6.4
BP084505                                                               25  8.3
BP064023                                                               25  8.3

>BP053584 
          Length = 514

 Score = 27.7 bits (60), Expect = 1.3
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = -1

Query: 139 CFSYYLHSCWLSEFSF 154
           C+ +  HSCWL  FSF
Sbjct: 373 CYEFIWHSCWLVMFSF 326


>TC9442 similar to GB|BAC55960.1|28071265|AB087408 actin binding protein
          {Arabidopsis thaliana;} , partial (9%)
          Length = 601

 Score = 26.6 bits (57), Expect = 2.9
 Identities = 16/39 (41%), Positives = 25/39 (64%), Gaps = 3/39 (7%)
 Frame = +3

Query: 60 FQEFKIKV---LEPGLVDHIVVSNKELAKIYVKNNESEV 95
          ++EF I V   L+ G+V  I +S+ +LAK Y+K   SE+
Sbjct: 21 YKEFGIPVNWLLDSGVVGKIKLSSVQLAKKYMKRVTSEL 137


>TC8427 homologue to UP|Q84L15 (Q84L15) Cytosolic glutamine synthetase,
           partial (41%)
          Length = 683

 Score = 26.6 bits (57), Expect = 2.9
 Identities = 14/46 (30%), Positives = 24/46 (51%)
 Frame = +1

Query: 1   MGNDKKKTNSQSLAQWMKVAVVGSFQEALKKLGTLMTLIGGLILSF 46
           +GN K+ T++ SL  W  V      +E  +K+G +     GL L++
Sbjct: 250 LGNMKQLTSTPSLGVWQTVEPQLELEEKQRKMGKVTLRTEGLPLTW 387


>TC19952 
          Length = 542

 Score = 26.2 bits (56), Expect = 3.7
 Identities = 18/50 (36%), Positives = 22/50 (44%), Gaps = 9/50 (18%)
 Frame = +3

Query: 125 LYVLLILLKPTGFRCFSY---------YLHSCWLSEFSFIIRNVLMEC*T 165
           L  LL L    GFRC+S+         YL S W+S    I+R     C T
Sbjct: 183 LATLLRLASEHGFRCYSWKMEMKILCMYLGSKWISVM*QILRKKFCGCWT 332


>TC9573 
          Length = 538

 Score = 25.4 bits (54), Expect = 6.4
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = -1

Query: 127 VLLILLKPTGFRCFSYYLH 145
           +LL+ L+    RCFS Y+H
Sbjct: 472 ILLLQLRRKNIRCFSIYIH 416


>TC17084 
          Length = 597

 Score = 25.4 bits (54), Expect = 6.4
 Identities = 28/100 (28%), Positives = 48/100 (48%), Gaps = 5/100 (5%)
 Frame = +1

Query: 76  IVVSNKELAKIYVKNNESEVAKYKYYFKIGS---VDSFERKLKKD*ALIIMILYVLLILL 132
           I  S +EL  + ++     V+K  Y+  +G    V  FE  +  D A +   +  +  +L
Sbjct: 181 IFASLEELFNLPLQTKLLNVSKKPYHGYVGQYPMVPLFE-SMGIDDANVSEKVEAMANIL 357

Query: 133 KPTGFRCFSYYLHSC--WLSEFSFIIRNVLMEC*TLEKHM 170
            P G   FS  ++S    LSE   IIR +++E   +EK++
Sbjct: 358 WPDGNPSFSKTINSFSEQLSELDHIIRKMVLESLGVEKYL 477


>BP084505 
          Length = 291

 Score = 25.0 bits (53), Expect = 8.3
 Identities = 8/13 (61%), Positives = 10/13 (76%)
 Frame = -1

Query: 140 FSYYLHSCWLSEF 152
           F +YL SCWL +F
Sbjct: 192 FCFYLFSCWLQKF 154


>BP064023 
          Length = 442

 Score = 25.0 bits (53), Expect = 8.3
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = -2

Query: 120 LIIMILYVLLILLKPTGFRCFSYYLHSCWLSEFSFI 155
           L+    Y+L  L+K   F  ++Y+L+      FS+I
Sbjct: 129 LVCEFPYLLPFLIKRPCFSMYAYFLYGTMFGPFSYI 22


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.337    0.148    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,925,544
Number of Sequences: 28460
Number of extensions: 37715
Number of successful extensions: 392
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 391
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 392
length of query: 188
length of database: 4,897,600
effective HSP length: 85
effective length of query: 103
effective length of database: 2,478,500
effective search space: 255285500
effective search space used: 255285500
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 52 (24.6 bits)


Medicago: description of AC146759.1