BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146758.11 + phase: 0 /pseudo
(1309 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP043850 48 9e-06
BI418295 47 1e-05
AV427609 41 0.001
AV779281 40 0.003
BP066067 39 0.004
AV780009 39 0.005
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 37 0.015
BP061908 37 0.026
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 36 0.035
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 35 0.059
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 35 0.077
TC11658 35 0.10
BP041457 35 0.10
TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting pr... 34 0.13
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 34 0.13
TC8301 34 0.17
AV777202 33 0.29
BP051686 32 0.50
BP073434 32 0.65
BI418821 31 1.5
>BP043850
Length = 515
Score = 48.1 bits (113), Expect = 9e-06
Identities = 37/91 (40%), Positives = 45/91 (48%)
Frame = -2
Query: 1214 FGFVVFWLNLDFLK*SQPHFMLTIQVPSKLLQILFSMSAPSISKLIVIPFVTHMMTD*YP 1273
FG VVF+ N +Q +MLT QVP KL QILF+M+ +LIV P M +
Sbjct: 505 FGCVVFFQNSVSYNHNQLRYMLTTQVPFKLQQILFTMNGRGT*RLIVTPSEKPMTAELSH 326
Query: 1274 FLMSVPNCKLQIFLPKLFRAHVISFLLAN*C 1304
+LMS KL P I LAN*C
Sbjct: 325 YLMSPHLFKLLTS*PNH*HVSAIIS*LAN*C 233
>BI418295
Length = 523
Score = 47.4 bits (111), Expect = 1e-05
Identities = 20/79 (25%), Positives = 42/79 (52%)
Frame = +2
Query: 47 APTEKAALEEWETKDAQIITWILSTINPQMINNLRSFSFAQEMWNYLKRIYNQDNPAKRF 106
A TE A W +D+ + TW+L+T++P ++ + + + ++W + + A+
Sbjct: 116 AATENPAYTTWIKQDSMVFTWLLTTLSPFVLPTVINCERSSQVWREILDFFENQCRAQSA 295
Query: 107 QLELEIANYKQGNSSVQEY 125
+L E+ N +G+ S+ EY
Sbjct: 296 KLRSELKNITKGSKSITEY 352
>AV427609
Length = 332
Score = 40.8 bits (94), Expect = 0.001
Identities = 23/92 (25%), Positives = 43/92 (46%), Gaps = 4/92 (4%)
Frame = +1
Query: 29 RMYVKGERLWSHLNGVSKAPTEKA---ALEEWETKDAQIITWILSTINPQMINN-LRSFS 84
R++ + R+ H+ + P A E+W T DA ++ WI STI+ ++ + S
Sbjct: 1 RIHARSHRVLHHIVPTADKPPPPVTDPAYEQWATLDATVLQWIYSTISVDLMTTIMEPGS 180
Query: 85 FAQEMWNYLKRIYNQDNPAKRFQLELEIANYK 116
A W +L ++ + A+ LE E + +
Sbjct: 181 TAFTAWTHLADLFQDNQNARAVTLEQEFSTVR 276
>AV779281
Length = 505
Score = 39.7 bits (91), Expect = 0.003
Identities = 24/117 (20%), Positives = 54/117 (45%), Gaps = 11/117 (9%)
Frame = +1
Query: 20 NYSAWEFQFRMYVKGERLWSHLNGVSKAP-----------TEKAALEEWETKDAQIITWI 68
N+ +W+ V+ R S L+ P E A + WE +D+ + TW+
Sbjct: 115 NFLSWKQHVEGIVRTHRWQSFLDHPDVPPRFLSDEDRVNAVENPAYQLWEQQDSALFTWL 294
Query: 69 LSTINPQMINNLRSFSFAQEMWNYLKRIYNQDNPAKRFQLELEIANYKQGNSSVQEY 125
LS+++P ++ + + S + ++ + + + A+ QL E+ + +G+ + +Y
Sbjct: 295 LSSLSPTVLPTVVNCSQSWQVREAVLDFFKAHSFAQSTQLRSELRSIAKGSKTCSDY 465
>BP066067
Length = 505
Score = 39.3 bits (90), Expect = 0.004
Identities = 26/101 (25%), Positives = 45/101 (43%), Gaps = 11/101 (10%)
Frame = -2
Query: 324 SHPWFLDSGASYHMTGSLEYLHNLHSYDGNKKIQIADGNTLSITDVG----------DIN 373
+H W DSGAS+H+T ++L + G +++ + G L I +G +
Sbjct: 306 THSW*ADSGASHHVTNDPQHLMESITMPGQEQVYVCSGQGLPILSIGFTSFSSPLISNTK 127
Query: 374 SDFRDVLVSPGLASNL-LSVGQLVVILMLIFHVLVVLCKSR 413
+D P + N+ + VG L +IL ++ KSR
Sbjct: 126 FSLKDFFHLPTITKNIVVLVGLLEIILSILVSSTKFNVKSR 4
>AV780009
Length = 529
Score = 38.9 bits (89), Expect = 0.005
Identities = 52/162 (32%), Positives = 69/162 (42%)
Frame = -2
Query: 1130 VVLFVT*REALTVAYSFPLEIRLS*VLIMMLIGQDALILDVLSLVGACFLALH*FHGKVR 1189
+V FV +E L + L + S I++L G A ILDVLSL FLA *FHG R
Sbjct: 519 LVFFVMSKEHLLKVFFSQLILH*SSKPIVILTGLVAPILDVLSLDIPYFLAPL*FHGARR 340
Query: 1190 NKQESPNLPLNLSIVLCLPLVQK*FGFVVFWLNLDFLK*SQPHFMLTIQVPSKLLQILFS 1249
NK PL SI L L K G + + + + +TI + LL I
Sbjct: 339 NKPLFLVHPLRQSIGPLLLLCVKSSGSPICSSSSSSMFHYRFLSFVTISLRFTLLTIRHF 160
Query: 1250 MSAPSISKLIVIPFVTHMMTD*YPFLMSVPNCKLQIFLPKLF 1291
P+ IV ++SVP+CKL + LF
Sbjct: 159 TRGPNT*NWIV--------------MLSVPSCKLA*SISCLF 76
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 37.4 bits (85), Expect = 0.015
Identities = 20/57 (35%), Positives = 26/57 (45%), Gaps = 4/57 (7%)
Frame = +1
Query: 228 GKGHDMQQVQCFSCKQFGHIARSCS----KKFCNYCKQRGHIITECYVRPPPSTQSP 280
G+G +CF+C GH AR C K C C +RGHI C P ++ P
Sbjct: 361 GRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKKLSRHP 531
>BP061908
Length = 472
Score = 36.6 bits (83), Expect = 0.026
Identities = 27/117 (23%), Positives = 54/117 (46%)
Frame = +3
Query: 54 LEEWETKDAQIITWILSTINPQMINNLRSFSFAQEMWNYLKRIYNQDNPAKRFQLELEIA 113
+++ E +D ++IT L N M+N L S F ++ N + + +L ++
Sbjct: 96 IQQKEKQDNKLITQFLHQDNKLMLNKLNSIPFIYDIHN---------S*LTKLKLNS*LS 248
Query: 114 NYKQGNSSVQEYYSGFLNLWTEHSAIIHADVPNIYLAAVQEVYNTSKQDQFLMILRP 170
+ N+ + SGF N H ++ V N L +V+N+S++ ++ + RP
Sbjct: 249 DGFNSNTRIG*KVSGFFNWGFPHWGFFNSGVFNF*LTFKLQVFNSSREREWWCVGRP 419
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 36.2 bits (82), Expect = 0.035
Identities = 15/36 (41%), Positives = 21/36 (57%), Gaps = 2/36 (5%)
Frame = +3
Query: 236 VQCFSCKQFGHIARSC--SKKFCNYCKQRGHIITEC 269
V C +C+Q GH++R C C+ C RGH+ EC
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 35.4 bits (80), Expect = 0.059
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = +2
Query: 226 RRGKGHDMQQVQCFSCKQFGHIARSC-SKKFCNYCKQRGHIITEC 269
RRG D C +CK+ GH AR C + C+ C GHI +EC
Sbjct: 317 RRGFSRDNL---CKNCKRPGHFARECPNVAICHNCGLPGHIASEC 442
Score = 33.5 bits (75), Expect = 0.22
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = +2
Query: 238 CFSCKQFGHIARSCS-KKFCNYCKQRGHIITEC 269
C +C GHIA C+ K C CK+ GH+ + C
Sbjct: 401 CHNCGLPGHIASECTTKSLCWNCKEPGHMASSC 499
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 35.0 bits (79), Expect = 0.077
Identities = 18/50 (36%), Positives = 22/50 (44%), Gaps = 4/50 (8%)
Frame = +2
Query: 228 GKGHDMQQVQCFSCKQFGHIARSCS----KKFCNYCKQRGHIITECYVRP 273
G+G +CF+C GH AR C K C C RGH+ C P
Sbjct: 341 GRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSP 490
>TC11658
Length = 528
Score = 34.7 bits (78), Expect = 0.10
Identities = 22/67 (32%), Positives = 36/67 (52%)
Frame = +3
Query: 652 LIIFLLWFLTLTLLSFVFSKLILIIVICILLGVCVLFIYLRLKGISLEHNLFSVHLWGIV 711
L++ LLW + + SF S L L V+ L+ C L YLR + N++ V+ +G V
Sbjct: 153 LLVGLLWLVARLISSFDCSYLQL*GVLGGLIMNCYLLFYLR--NFGYDENIWQVYCFGNV 326
Query: 712 TFIRDLC 718
I+++C
Sbjct: 327 DNIKEVC 347
>BP041457
Length = 495
Score = 34.7 bits (78), Expect = 0.10
Identities = 26/126 (20%), Positives = 51/126 (39%), Gaps = 15/126 (11%)
Frame = +2
Query: 15 RFSGKNYSAWEFQFRMYVKGERLWSHLNGVSKAPTEKAALEE-----------WETKDAQ 63
+ S K + W+ Q +K L ++ + P + E WET D
Sbjct: 122 KLSAKKFLLWKQQVEPIIKAHHLHCYIVEPAIPPRYSSDEEHANGVVNPAYPIWETHDQF 301
Query: 64 IITW----ILSTINPQMINNLRSFSFAQEMWNYLKRIYNQDNPAKRFQLELEIANYKQGN 119
+++W + S+++ MI + SF ++W + ++ AK Q+ E+ + K G
Sbjct: 302 LLSWLQSLLSSSVHTCMIGCVNSF----QLWVKIHSFFHNQTRAKSTQIRTELRSAKLGG 469
Query: 120 SSVQEY 125
+ Y
Sbjct: 470 RLLHGY 487
>TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting protein 102
(Fragment), partial (13%)
Length = 667
Score = 34.3 bits (77), Expect = 0.13
Identities = 36/111 (32%), Positives = 60/111 (53%), Gaps = 2/111 (1%)
Frame = -2
Query: 648 LCS*LIIFLLWFLTLTLLSFV--FSKLILIIVICILLGVCVLFIYLRLKGISLEHNLFSV 705
LC + +FL+ LT+L V FS +IL I++ +LL LF+ L+ + + +F++
Sbjct: 345 LCVLVFLFLMLLFALTVLLSVTNFSSIILSILVFLLL----LFL---LRLV*IW*TIFNL 187
Query: 706 HLWGIVTFIRDLCFMMSLIIASEFRGMLHFLIINSCFILFLLT*MILLFFP 756
+L ++ F+ L F LI F +L L+ +LFLLT + L+FP
Sbjct: 186 YL--VLLFL--LLFSFLLI*RCHFTILLVLLLF--LLLLFLLTLNLHLYFP 52
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 34.3 bits (77), Expect = 0.13
Identities = 18/65 (27%), Positives = 27/65 (40%)
Frame = -2
Query: 232 DMQQVQCFSCKQFGHIARSCSKKFCNYCKQRGHIITECYVRPPPSTQSPM*ALHAYSTTN 291
D ++ C C + GH A C C C++ GH +C P ++ A+ A
Sbjct: 347 DTSEIVCHRCSKKGHFANRCPDLVCWNCQKTGHSGKDC---TNPKVEAATNAIAARRPAP 177
Query: 292 ATNGG 296
A N G
Sbjct: 176 AANKG 162
>TC8301
Length = 1243
Score = 33.9 bits (76), Expect = 0.17
Identities = 15/83 (18%), Positives = 42/83 (50%)
Frame = +2
Query: 15 RFSGKNYSAWEFQFRMYVKGERLWSHLNGVSKAPTEKAALEEWETKDAQIITWILSTINP 74
+FSG N+S W+ + + ++ + ++G T+ ++W+ D + + +
Sbjct: 524 KFSGSNFSLWKLKIKAILRKDNCLPAIDGRPADITD----DKWKEMDDNAVANLHLAVAD 691
Query: 75 QMINNLRSFSFAQEMWNYLKRIY 97
+++++ A+E+W+ L ++Y
Sbjct: 692 SVLSSIAEKKTAKEIWDTLIQLY 760
>AV777202
Length = 421
Score = 33.1 bits (74), Expect = 0.29
Identities = 17/97 (17%), Positives = 43/97 (43%), Gaps = 11/97 (11%)
Frame = +3
Query: 5 SQKQIDNMCVRFSGKNYSAWEFQFRMYVKGERLWSHLNGVSKAP-----------TEKAA 53
S + + ++ N+ +W+ V+ RL + L P E A
Sbjct: 81 SSSLVHKLVLKLDDSNFLSWKQHVEGIVRTHRLQNFLVHPDIPPRFLSEEDRVNAVENPA 260
Query: 54 LEEWETKDAQIITWILSTINPQMINNLRSFSFAQEMW 90
+ W+ +D+ + TW+LS+++ ++ + + + + ++W
Sbjct: 261 YQVWDQQDSALFTWLLSSLSSSVLPTVVNCTQSWQIW 371
>BP051686
Length = 502
Score = 32.3 bits (72), Expect = 0.50
Identities = 20/69 (28%), Positives = 37/69 (52%), Gaps = 9/69 (13%)
Frame = -1
Query: 1065 QWILLLRLMLNIIVMMVVSCLI---------RYCIDNLWVVLII*LLLVLTYLLLFNK*V 1115
+WIL+ + N++++ C++ YCI +LW V + L L++T L+LF +
Sbjct: 418 EWILVYNVA-NLLMLPYH*CMLA*M*KKE*WNYCIVSLWFVCFLCLNLLMTLLVLF---I 251
Query: 1116 NSCILHAIF 1124
C+ A+F
Sbjct: 250 LLCLFFAVF 224
Score = 28.5 bits (62), Expect = 7.2
Identities = 11/36 (30%), Positives = 21/36 (57%)
Frame = -1
Query: 654 IFLLWFLTLTLLSFVFSKLILIIVICILLGVCVLFI 689
I LWF+ L+ + + L+L I++C+ V ++ I
Sbjct: 319 IVSLWFVCFLCLNLLMTLLVLFILLCLFFAVFLVLI 212
>BP073434
Length = 490
Score = 32.0 bits (71), Expect = 0.65
Identities = 37/146 (25%), Positives = 58/146 (39%), Gaps = 1/146 (0%)
Frame = -2
Query: 327 WFLDSGASYHMTGSLEYLHNLHSYDGNKKIQIADGNTLSITDVGDINSDFRDVLVSPGLA 386
W+ DSGAS+H+T + ++G I + +G LS+ G F +
Sbjct: 456 WYPDSGASHHVTPHGNNIQYPDPFEGPDHIFVGNGQGLSVASSGFSPQSFS*AMWE---- 289
Query: 387 SNLLSVGQLVVILMLIFHVLVVLCKSRCRGKLSRRGLKWEDCFLFSL-FPISYLLLVTMF 445
L V + +L+F VL+ D L +L P LLL++
Sbjct: 288 ---LMVYTPFLAYLLLFPVLI-------------------DLHLSTLSIP*LLLLLIS-- 181
Query: 446 *ILMRTGIENWVIQTLLFCLIYLNLV 471
+GI VI T + C ++ NLV
Sbjct: 180 ---S*SGIAG*VILTRMLCKLFFNLV 112
>BI418821
Length = 614
Score = 30.8 bits (68), Expect = 1.5
Identities = 12/41 (29%), Positives = 19/41 (46%), Gaps = 9/41 (21%)
Frame = +2
Query: 238 CFSCKQFGHIARSCSKK---------FCNYCKQRGHIITEC 269
C++C GH+AR C++ C C GH+ +C
Sbjct: 476 CYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDC 598
Score = 30.4 bits (67), Expect = 1.9
Identities = 15/55 (27%), Positives = 22/55 (39%), Gaps = 10/55 (18%)
Frame = +2
Query: 225 QRRGKGHDMQQVQCFSCKQFGHIARSCSKK----------FCNYCKQRGHIITEC 269
+R G G C++C GH+AR C + C C GH+ +C
Sbjct: 353 RRNGGGGGGGGGGCYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDC 517
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.347 0.154 0.508
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,266,118
Number of Sequences: 28460
Number of extensions: 439791
Number of successful extensions: 5225
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 4998
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5206
length of query: 1309
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1208
effective length of database: 2,023,140
effective search space: 2443953120
effective search space used: 2443953120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146758.11