
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146757.7 - phase: 0
(367 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19879 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kin... 304 1e-83
TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase ki... 221 2e-58
TC11331 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kin... 126 7e-30
BP085705 30 0.83
TC17163 29 1.1
TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, p... 28 1.8
TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta... 28 1.8
TC15421 similar to GB|AAL36081.1|17380636|AY063731 AT3g49800/T16... 27 5.4
TC12118 26 9.2
>TC19879 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kinase ,
partial (47%)
Length = 614
Score = 304 bits (779), Expect = 1e-83
Identities = 143/178 (80%), Positives = 166/178 (92%)
Frame = +2
Query: 3 AIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIARRA 62
A E FSK+L+++VH+WGC+KQTGVSLRYMMEFGS PT+KNLLISAQFL KELAIRIARRA
Sbjct: 80 ACETFSKALMEDVHRWGCMKQTGVSLRYMMEFGSTPTEKNLLISAQFLHKELAIRIARRA 259
Query: 63 IELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNNVV 122
IEL+SLPYGLSKKPA++KVRDWYVDSFRD+RS P++K+M DE++FT++IKAIKVRHNNVV
Sbjct: 260 IELDSLPYGLSKKPAVIKVRDWYVDSFRDLRSYPDIKNMTDEKDFTEMIKAIKVRHNNVV 439
Query: 123 PTMALGVQQLKKELKTKIDSEDLVEIHEFLDRFYLSRIGVRMLIGQHVELHNPNPPPH 180
PTMALGVQQLKK + KI EDL+EIH+FLDRFY+SRIG+RMLIGQHVELHNPNP PH
Sbjct: 440 PTMALGVQQLKKGMDPKIVYEDLIEIHQFLDRFYMSRIGIRMLIGQHVELHNPNPAPH 613
>TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase kinase ,
partial (31%)
Length = 538
Score = 221 bits (562), Expect = 2e-58
Identities = 106/118 (89%), Positives = 114/118 (95%)
Frame = +1
Query: 250 ERYMDSDKVSPPIRIIVADGLEDVTIKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHAD 309
ER++DSD V+PP+RIIVADGLEDVTIK+SDEGGGIPRSGLPKIFTYLYSTARNPLDEH D
Sbjct: 1 ERFIDSDNVAPPVRIIVADGLEDVTIKVSDEGGGIPRSGLPKIFTYLYSTARNPLDEHED 180
Query: 310 LGVADSVTTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 367
LG+ D+V TMAGYGYG+PISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP
Sbjct: 181 LGLGDNV-TMAGYGYGIPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 351
>TC11331 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kinase ,
partial (21%)
Length = 384
Score = 126 bits (316), Expect = 7e-30
Identities = 60/72 (83%), Positives = 68/72 (94%)
Frame = +2
Query: 3 AIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIARRA 62
A E FSK+L+++VH+WGC+KQTGVSLRYMMEFGS PT+KNLLISAQFL KELAIRIARRA
Sbjct: 167 ACETFSKALMEDVHRWGCMKQTGVSLRYMMEFGSTPTEKNLLISAQFLHKELAIRIARRA 346
Query: 63 IELESLPYGLSK 74
IEL+SLPYGLSK
Sbjct: 347 IELDSLPYGLSK 382
>BP085705
Length = 358
Score = 29.6 bits (65), Expect = 0.83
Identities = 21/59 (35%), Positives = 26/59 (43%), Gaps = 7/59 (11%)
Frame = +2
Query: 131 QLKKELKTKIDSEDLVEIHEFLDRFYLSRIGVRMLIGQ-------HVELHNPNPPPHVV 182
QLK E+ T + H+ + S IGVR Q H HNPNPPP +V
Sbjct: 119 QLKNEIITTPNVYVSGRKHQLIGVHSYSLIGVRSYSSQGPGQYSHHHRHHNPNPPPLMV 295
>TC17163
Length = 514
Score = 29.3 bits (64), Expect = 1.1
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -3
Query: 155 FYLSRIGVRMLIGQHVELHNPNPPP 179
FYL R+ + +L+ +E NPN PP
Sbjct: 338 FYLHRVKITILMSHKLEKKNPNSPP 264
>TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, partial (8%)
Length = 769
Score = 28.5 bits (62), Expect = 1.8
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = +2
Query: 169 HVELHNPNPPPHVVGYIHTKMSP 191
H +H P+PP HV Y H SP
Sbjct: 263 HDHVHQPSPPQHVNQYSHYPTSP 331
>TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (18%)
Length = 680
Score = 28.5 bits (62), Expect = 1.8
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = +2
Query: 324 YGLPISRLYARYFGGD 339
Y LP R+YA YFGGD
Sbjct: 524 YKLPSDRIYATYFGGD 571
>TC15421 similar to GB|AAL36081.1|17380636|AY063731 AT3g49800/T16K5_150
{Arabidopsis thaliana;}, partial (44%)
Length = 856
Score = 26.9 bits (58), Expect = 5.4
Identities = 13/50 (26%), Positives = 24/50 (48%)
Frame = +3
Query: 165 LIGQHVELHNPNPPPHVVGYIHTKMSPVSVARNASEDARSICMREYGSAP 214
L H +H+P+P PH++ P + +R S + + YG++P
Sbjct: 126 LTSNHNPIHHPHPHPHLL-----TAPPHAASRKISPSSPNPSRASYGASP 260
>TC12118
Length = 583
Score = 26.2 bits (56), Expect = 9.2
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -3
Query: 173 HNPNPPPHVVGYIHTKMSPVSVARNAS 199
HNP+P P+ + I K PV + N S
Sbjct: 191 HNPSPSPYTILSIIFKPKPVGLTSNKS 111
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.139 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,482,598
Number of Sequences: 28460
Number of extensions: 68772
Number of successful extensions: 381
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 380
length of query: 367
length of database: 4,897,600
effective HSP length: 92
effective length of query: 275
effective length of database: 2,279,280
effective search space: 626802000
effective search space used: 626802000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146757.7