Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146757.7 - phase: 0 
         (367 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC19879 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kin...   304  1e-83
TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase ki...   221  2e-58
TC11331 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kin...   126  7e-30
BP085705                                                               30  0.83
TC17163                                                                29  1.1
TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, p...    28  1.8
TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta...    28  1.8
TC15421 similar to GB|AAL36081.1|17380636|AY063731 AT3g49800/T16...    27  5.4
TC12118                                                                26  9.2

>TC19879 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kinase  ,
           partial (47%)
          Length = 614

 Score =  304 bits (779), Expect = 1e-83
 Identities = 143/178 (80%), Positives = 166/178 (92%)
 Frame = +2

Query: 3   AIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIARRA 62
           A E FSK+L+++VH+WGC+KQTGVSLRYMMEFGS PT+KNLLISAQFL KELAIRIARRA
Sbjct: 80  ACETFSKALMEDVHRWGCMKQTGVSLRYMMEFGSTPTEKNLLISAQFLHKELAIRIARRA 259

Query: 63  IELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNNVV 122
           IEL+SLPYGLSKKPA++KVRDWYVDSFRD+RS P++K+M DE++FT++IKAIKVRHNNVV
Sbjct: 260 IELDSLPYGLSKKPAVIKVRDWYVDSFRDLRSYPDIKNMTDEKDFTEMIKAIKVRHNNVV 439

Query: 123 PTMALGVQQLKKELKTKIDSEDLVEIHEFLDRFYLSRIGVRMLIGQHVELHNPNPPPH 180
           PTMALGVQQLKK +  KI  EDL+EIH+FLDRFY+SRIG+RMLIGQHVELHNPNP PH
Sbjct: 440 PTMALGVQQLKKGMDPKIVYEDLIEIHQFLDRFYMSRIGIRMLIGQHVELHNPNPAPH 613


>TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase kinase  ,
           partial (31%)
          Length = 538

 Score =  221 bits (562), Expect = 2e-58
 Identities = 106/118 (89%), Positives = 114/118 (95%)
 Frame = +1

Query: 250 ERYMDSDKVSPPIRIIVADGLEDVTIKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHAD 309
           ER++DSD V+PP+RIIVADGLEDVTIK+SDEGGGIPRSGLPKIFTYLYSTARNPLDEH D
Sbjct: 1   ERFIDSDNVAPPVRIIVADGLEDVTIKVSDEGGGIPRSGLPKIFTYLYSTARNPLDEHED 180

Query: 310 LGVADSVTTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 367
           LG+ D+V TMAGYGYG+PISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP
Sbjct: 181 LGLGDNV-TMAGYGYGIPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 351


>TC11331 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kinase  ,
           partial (21%)
          Length = 384

 Score =  126 bits (316), Expect = 7e-30
 Identities = 60/72 (83%), Positives = 68/72 (94%)
 Frame = +2

Query: 3   AIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIARRA 62
           A E FSK+L+++VH+WGC+KQTGVSLRYMMEFGS PT+KNLLISAQFL KELAIRIARRA
Sbjct: 167 ACETFSKALMEDVHRWGCMKQTGVSLRYMMEFGSTPTEKNLLISAQFLHKELAIRIARRA 346

Query: 63  IELESLPYGLSK 74
           IEL+SLPYGLSK
Sbjct: 347 IELDSLPYGLSK 382


>BP085705 
          Length = 358

 Score = 29.6 bits (65), Expect = 0.83
 Identities = 21/59 (35%), Positives = 26/59 (43%), Gaps = 7/59 (11%)
 Frame = +2

Query: 131 QLKKELKTKIDSEDLVEIHEFLDRFYLSRIGVRMLIGQ-------HVELHNPNPPPHVV 182
           QLK E+ T  +       H+ +     S IGVR    Q       H   HNPNPPP +V
Sbjct: 119 QLKNEIITTPNVYVSGRKHQLIGVHSYSLIGVRSYSSQGPGQYSHHHRHHNPNPPPLMV 295


>TC17163 
          Length = 514

 Score = 29.3 bits (64), Expect = 1.1
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = -3

Query: 155 FYLSRIGVRMLIGQHVELHNPNPPP 179
           FYL R+ + +L+   +E  NPN PP
Sbjct: 338 FYLHRVKITILMSHKLEKKNPNSPP 264


>TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, partial (8%)
          Length = 769

 Score = 28.5 bits (62), Expect = 1.8
 Identities = 11/23 (47%), Positives = 13/23 (55%)
 Frame = +2

Query: 169 HVELHNPNPPPHVVGYIHTKMSP 191
           H  +H P+PP HV  Y H   SP
Sbjct: 263 HDHVHQPSPPQHVNQYSHYPTSP 331


>TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
           [imported] - Arabidopsis                thaliana
           {Arabidopsis thaliana;}, partial (18%)
          Length = 680

 Score = 28.5 bits (62), Expect = 1.8
 Identities = 11/16 (68%), Positives = 12/16 (74%)
 Frame = +2

Query: 324 YGLPISRLYARYFGGD 339
           Y LP  R+YA YFGGD
Sbjct: 524 YKLPSDRIYATYFGGD 571


>TC15421 similar to GB|AAL36081.1|17380636|AY063731 AT3g49800/T16K5_150
           {Arabidopsis thaliana;}, partial (44%)
          Length = 856

 Score = 26.9 bits (58), Expect = 5.4
 Identities = 13/50 (26%), Positives = 24/50 (48%)
 Frame = +3

Query: 165 LIGQHVELHNPNPPPHVVGYIHTKMSPVSVARNASEDARSICMREYGSAP 214
           L   H  +H+P+P PH++        P + +R  S  + +     YG++P
Sbjct: 126 LTSNHNPIHHPHPHPHLL-----TAPPHAASRKISPSSPNPSRASYGASP 260


>TC12118 
          Length = 583

 Score = 26.2 bits (56), Expect = 9.2
 Identities = 11/27 (40%), Positives = 15/27 (54%)
 Frame = -3

Query: 173 HNPNPPPHVVGYIHTKMSPVSVARNAS 199
           HNP+P P+ +  I  K  PV +  N S
Sbjct: 191 HNPSPSPYTILSIIFKPKPVGLTSNKS 111


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.320    0.139    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,482,598
Number of Sequences: 28460
Number of extensions: 68772
Number of successful extensions: 381
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 380
length of query: 367
length of database: 4,897,600
effective HSP length: 92
effective length of query: 275
effective length of database: 2,279,280
effective search space: 626802000
effective search space used: 626802000
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)


Medicago: description of AC146757.7