BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146757.4 - phase: 0 /pseudo
(755 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780705 92 4e-19
AV767121 91 5e-19
AV780221 69 2e-12
BP060613 59 4e-09
BP085058 57 1e-08
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 47 1e-05
BE122611 42 5e-04
TC18710 39 0.003
TC12829 32 0.068
TC13572 31 0.83
BP033834 29 3.2
TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane pro... 28 5.4
CB826728 28 7.1
BI420752 27 9.2
AV767022 27 9.2
BP028574 27 9.2
>AV780705
Length = 524
Score = 91.7 bits (226), Expect = 4e-19
Identities = 51/143 (35%), Positives = 70/143 (48%), Gaps = 1/143 (0%)
Frame = -2
Query: 137 GMYLGANLAPGRSLRGKFNHIVNKIQSKLNGWKQQCLTLAGRITLAKSVISTIPYYHMQY 196
G YLG GR+ I K++ KL GWK+ L AG+ L K++I IP Y M
Sbjct: 490 GHYLGLPAIWGRNKSHSLVWIEEKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYAMTI 311
Query: 197 AKIPKTICDEIDKIQRGFIWGDSDQGRKAHLISWDVC-CLPKADGGLGFRQTHKMNEAFL 255
PKT C+ + + F W +G + + L K GG+GFR N AFL
Sbjct: 310 VHFPKTFCNSLYALVADFWWKSQGEGAVEYTGAVGTK*PLSKEKGGVGFRDLRTQNLAFL 131
Query: 256 MKILWNLIKNPEDLWCRVLRSKY 278
+ W ++ NPE LW RV++S Y
Sbjct: 130 ARQAWRVLTNPEALWVRVMKSLY 62
>AV767121
Length = 525
Score = 91.3 bits (225), Expect = 5e-19
Identities = 49/173 (28%), Positives = 83/173 (47%), Gaps = 7/173 (4%)
Frame = +2
Query: 165 LNGWKQQCLTLAGRITLAKSVISTIPYYHMQYAKIPKTICDEIDKIQRGFIWGDSDQGRK 224
L+ WKQ+ ++ GRI L +SV++ +P + + + K+P + ++ R F+WG S+ K
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENK 181
Query: 225 AHLISWDVCCLPKADGGLGFRQTHKMNEAFLMKILWNLIKNPEDLWCRVLRSKYGRN--- 281
+ W C PK GGLG + N+A L K W + P+ LW RV+ ++
Sbjct: 182 IA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVIEAQPDHYSCG 361
Query: 282 ----NDLIASINAHPYDSPLWKALVNIWNDFKGHVVWNIGDGRNTNFWLDKWV 330
ND+++ P D+ W F + +G+G T FW + W+
Sbjct: 362 SSWWNDILSLC---PEDADGW---------FSSGLKKLVGEGDQTKFWSEDWL 484
>AV780221
Length = 461
Score = 69.3 bits (168), Expect = 2e-12
Identities = 45/166 (27%), Positives = 70/166 (42%), Gaps = 14/166 (8%)
Frame = +3
Query: 179 ITLAKSVISTIPYYHMQYAKIPKT-ICDEIDKIQRGFIWGDSDQGRKAHLISWDVCCLPK 237
+ L +SV+ +P +H+ + K+P ++ F + + G+K + W C PK
Sbjct: 3 LCLIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGGKKIAWVKWSTVCRPK 182
Query: 238 ADGGLGFRQTHKMNEAFLMKILWNLIKNPEDLWCRVLRSKYGRNNDLIASINAHPYDSPL 297
+GGLG + N+A L K W ++K + LW +VL KY NA P +
Sbjct: 183 DEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYK---------NAIPQSASS 335
Query: 298 WKALVNIWNDF------KGHVVW-------NIGDGRNTNFWLDKWV 330
W WND G W IG+G FW + W+
Sbjct: 336 W------WNDLHSTCFEDGGGGWMQRGLCRRIGEGTEVKFWNENWL 455
>BP060613
Length = 378
Score = 58.5 bits (140), Expect = 4e-09
Identities = 30/105 (28%), Positives = 51/105 (48%)
Frame = +1
Query: 215 IWGDSDQGRKAHLISWDVCCLPKADGGLGFRQTHKMNEAFLMKILWNLIKNPEDLWCRVL 274
+W S R H WD+ K +GGLGF+ N+A L K W ++ NP+ LW ++L
Sbjct: 43 VWWSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQIL 222
Query: 275 RSKYGRNNDLIASINAHPYDSPLWKALVNIWNDFKGHVVWNIGDG 319
++ Y ++D + + S +W +L++ W +G G
Sbjct: 223 KALYFPHHDFLQT-TKRTGASWVWSSLLHGRELLLKQGQWQLGSG 354
>BP085058
Length = 452
Score = 57.0 bits (136), Expect = 1e-08
Identities = 36/129 (27%), Positives = 65/129 (49%)
Frame = +2
Query: 116 VDTQLREDILHHTGFNQVNSLGMYLGANLAPGRSLRGKFNHIVNKIQSKLNGWKQQCLTL 175
++ LR+ ++ T +++G Y+ + G + F ++++ S+L K L
Sbjct: 68 ININLRDRMVSITSIPFTDNIGKYMSFPIHQGHV*KEDFVFTLDRVISRLAC*KAYLLNK 247
Query: 176 AGRITLAKSVISTIPYYHMQYAKIPKTICDEIDKIQRGFIWGDSDQGRKAHLISWDVCCL 235
R+TLAK V+S I Y MQ +P++ICD+I + R F+W + L++W+
Sbjct: 248 PARLTLAKPVLSAISMYVMQLNWLPQSICDQICTVVRHFVW---ENVNGIPLVAWETIQS 418
Query: 236 PKADGGLGF 244
K+ GL F
Sbjct: 419 RKSMVGLIF 445
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 46.6 bits (109), Expect = 1e-05
Identities = 34/123 (27%), Positives = 57/123 (45%)
Frame = -3
Query: 64 QISHLLFADDLLLFAEASIEQAHCVLHCLDIFCQASGQKINRNKTCVYFSKNVDTQLRED 123
+ISHL FAD+L++F+ +E + + L IF SG N K+ V+ V +
Sbjct: 376 EISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSEVFIL--VKETTKNQ 203
Query: 124 ILHHTGFNQVNSLGMYLGANLAPGRSLRGKFNHIVNKIQSKLNGWKQQCLTLAGRITLAK 183
I + G+ + LG + +V++ SKL W + L+ AGR+ L
Sbjct: 202 ITNMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLIN 23
Query: 184 SVI 186
S++
Sbjct: 22 SIL 14
>BE122611
Length = 357
Score = 41.6 bits (96), Expect = 5e-04
Identities = 23/91 (25%), Positives = 43/91 (46%)
Frame = -3
Query: 389 GPDTIGWGGTNTHHFTVQSAYSLQHQDCPILEGDWKSIWKWHGPHRIQTFIWLDTHERIL 448
G D I W + ++ +S Y+L +D W +WK + P +++ F+WL +
Sbjct: 337 GTDRILWTEESNGCYSAKSTYNLLIEDGGNESRFWSLVWKANAPEKVRFFLWLVGRNALP 158
Query: 449 TNFQRSNGVVEYLLIARIVEMKMKLFFMCFA 479
+N +R + + L + VE+ + M FA
Sbjct: 157 SNARRHHITLLVLQLVSAVELLRRTRTMYFA 65
>TC18710
Length = 843
Score = 38.9 bits (89), Expect = 0.003
Identities = 19/43 (44%), Positives = 24/43 (55%)
Frame = +2
Query: 2 IEYIIMWNGEKTDTFFSTRGIRQGDPLSPYLFVICMDRLSHMI 44
+ Y NG RG+RQGDP SPYLF+ M+ LS +I
Sbjct: 686 VSYNYKINGVVGPKLLPQRGLRQGDPFSPYLFLFTMEVLSLLI 814
>TC12829
Length = 448
Score = 32.0 bits (71), Expect(2) = 0.068
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3
Query: 170 QQCLTLAGRITLAKSVISTIPYYHMQYAKIPKTICDEID 208
++ L AGR L KSV IP Y M +P +IC +I+
Sbjct: 252 EKFLYRAGREVLIKSVTKAIPTYIMSCFALPDSICSQIE 368
Score = 21.2 bits (43), Expect(2) = 0.068
Identities = 7/21 (33%), Positives = 8/21 (37%)
Frame = +1
Query: 214 FIWGDSDQGRKAHLISWDVCC 234
FIW R H + W C
Sbjct: 385 FIWSGDVSRRSIHWLGWKKLC 447
>TC13572
Length = 534
Score = 30.8 bits (68), Expect = 0.83
Identities = 19/60 (31%), Positives = 28/60 (46%)
Frame = -3
Query: 270 WCRVLRSKYGRNNDLIASINAHPYDSPLWKALVNIWNDFKGHVVWNIGDGRNTNFWLDKW 329
WCRV+ +KYG +I++ D N W + H V + G +T FWL+ W
Sbjct: 478 WCRVVLAKYGDGG--CTNISSWWRDVQTAGGGENGWFEEGVHKV--LRSGNHTRFWLENW 311
>BP033834
Length = 534
Score = 28.9 bits (63), Expect = 3.2
Identities = 12/40 (30%), Positives = 24/40 (60%)
Frame = +1
Query: 226 HLISWDVCCLPKADGGLGFRQTHKMNEAFLMKILWNLIKN 265
HL+SW K GLGFR+ K + +++ ++++ +K+
Sbjct: 25 HLVSWGKATHVKNY*GLGFREARKDHRKYVIHLVYHHLKS 144
>TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane protein-like,
partial (10%)
Length = 879
Score = 28.1 bits (61), Expect = 5.4
Identities = 16/57 (28%), Positives = 25/57 (43%), Gaps = 6/57 (10%)
Frame = -1
Query: 359 GDWNIDFLINNLPTNTVNQILA------LSIPNDDDGPDTIGWGGTNTHHFTVQSAY 409
G W F+ P+ N ++A S+P +D D+ W +TVQ+AY
Sbjct: 774 GVWTWSFIRRRNPSTRENLLVADLCNLLESVPLSEDTNDSWSWTANPEGQYTVQTAY 604
>CB826728
Length = 509
Score = 27.7 bits (60), Expect = 7.1
Identities = 16/58 (27%), Positives = 28/58 (47%)
Frame = +3
Query: 327 DKWVPNNESLMSIGNQTYMDITLSVRDVLSPSGDWNIDFLINNLPTNTVNQILALSIP 384
D W+PN+ L+ ++D+ L L +G WN + + +T LA+S+P
Sbjct: 15 DAWLPNSAPLVYC-EDLFVDLQLKKVADLIENGRWNENLVSMVFSPSTTAVFLAVSLP 185
>BI420752
Length = 593
Score = 27.3 bits (59), Expect = 9.2
Identities = 21/80 (26%), Positives = 33/80 (41%), Gaps = 18/80 (22%)
Frame = -1
Query: 390 PDTIGWGGTNTHH----------FTVQSAYSLQHQDCPIL--------EGDWKSIWKWHG 431
P+T+ THH F + +L H CP+L EG +K+H
Sbjct: 434 PETMAAPSAETHHPSRYCPDEPHFCHNTGAALSH--CPLLQGKPQQRVEGQRPQAYKFHA 261
Query: 432 PHRIQTFIWLDTHERILTNF 451
P+R T + H+ ++ NF
Sbjct: 260 PNRSSTQL-SQAHKLLILNF 204
>AV767022
Length = 289
Score = 27.3 bits (59), Expect = 9.2
Identities = 13/32 (40%), Positives = 14/32 (43%)
Frame = +1
Query: 399 NTHHFTVQSAYSLQHQDCPILEGDWKSIWKWH 430
N+ H TVQ L P L DW KWH
Sbjct: 127 NSPHKTVQGGLYLPDLTKPFLSYDWNPKIKWH 222
>BP028574
Length = 454
Score = 27.3 bits (59), Expect = 9.2
Identities = 20/79 (25%), Positives = 32/79 (40%), Gaps = 1/79 (1%)
Frame = +3
Query: 363 IDFLINNLPTNTVNQILALSIPNDDDGPDTIGWGGTNTHHFTVQSAYSLQHQDCPILEGD 422
IDF+ L + LSI + D W + FTV+SAY H + +E +
Sbjct: 189 IDFIYLKLKVTQT*NVKVLSINHRDSWV----WIKDGSGSFTVKSAYEELHGEIQEIEDN 356
Query: 423 -WKSIWKWHGPHRIQTFIW 440
++ +W+ P W
Sbjct: 357 FFRKLWQSKAPSNSIALAW 413
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.338 0.149 0.503
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,035,217
Number of Sequences: 28460
Number of extensions: 232792
Number of successful extensions: 1839
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1783
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1824
length of query: 755
length of database: 4,897,600
effective HSP length: 97
effective length of query: 658
effective length of database: 2,136,980
effective search space: 1406132840
effective search space used: 1406132840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146757.4