BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146757.2 + phase: 0
(1715 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 231 4e-66
BP084332 192 5e-49
AV780009 130 1e-30
BP052632 103 2e-22
BP075943 79 8e-15
BP057233 70 4e-12
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 69 5e-12
BP063694 65 9e-11
AV766665 63 4e-10
BP043850 62 6e-10
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 47 1e-09
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 60 3e-09
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 59 6e-09
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 58 1e-08
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 57 2e-08
AV427921 56 5e-08
AU088980 55 9e-08
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 55 9e-08
AV424544 49 7e-06
TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retro... 35 2e-05
>BP066094
Length = 532
Score = 231 bits (589), Expect(3) = 4e-66
Identities = 111/149 (74%), Positives = 133/149 (88%)
Frame = +2
Query: 1293 ARLEAIRLLLSYAINHGIILYQMDVKSAFLNGVIEEEVYVKQPPGFEDLKHPDHVYKLKK 1352
++ EAIRLL+S+++NH IIL+QM+VKSAFLNG I EEVYV QPPG ED K+ DH++KLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1353 SLYGLKQAPRAWYDRLSNFLIKNDFERGQVDTTLFRKTLKKDILIVQIYVDDIIFGSSNA 1412
SLYGLKQAPRAWY+RLS+FL++N+ RG+VDTTLF KT K DILIVQIYVDDIIFGS+N
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1413 SLCKEFSKLMQDEFEMSMMGELKFFMGIQ 1441
SLCKEFS++MQ EFEM MMGELK+F+GIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 4e-66
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 1277 YSQQEGIDYTETFAPVAR 1294
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 4e-66
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1267 RNKARLVAQG 1276
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 192 bits (487), Expect = 5e-49
Identities = 89/112 (79%), Positives = 104/112 (92%)
Frame = +2
Query: 1597 QATIAMSTAEAEYISAASCCTQLLWMKHQLEDYQINANSIPIYCDNTAAICLSKNPILHS 1656
Q+TIA+STAEAEYISAA C TQ+LWMKHQLEDYQI ++IPIYCDNTAAI LSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1657 RAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLSVERFDFIKKNL 1708
RAKHIE+K+HFIRDYVQKG+L ++F+DT+HQWADIFTKPL+ +RF+FI KNL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
>AV780009
Length = 529
Score = 130 bits (328), Expect = 1e-30
Identities = 63/163 (38%), Positives = 100/163 (60%), Gaps = 1/163 (0%)
Frame = -1
Query: 1534 AVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLISWA 1593
A R+ RY+KG GL + KL + D+D+AG R+S +G FLG +LISW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1594 SKRQATIAMSTAEAEYISAASCCTQLLWMKHQLEDYQINAN-SIPIYCDNTAAICLSKNP 1652
+K+Q T++ S++EAEY + A+ ++ W+ + + ++N +P++CDN +A+ ++ NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 1653 ILHSRAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKP 1695
H R KHIE+ H +R +Q G++ + I T HQ ADIFTKP
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 103 bits (257), Expect = 2e-22
Identities = 56/124 (45%), Positives = 74/124 (59%), Gaps = 1/124 (0%)
Frame = -3
Query: 1011 KTAYELFKGRRPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNS 1070
KT YEL++GRRP +SYFH FGC +ILNT D + KFD K + I LGYS SKAY V+NS
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 1071 ETQCVEESMHVKFDD-REPGSKTSEQSESNAGTTDSEDASESDQPSDSEKYTEVESSPEA 1129
TQ VEES++VKFDD + E+ N +D ++ +++ SE E S+
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEEPSTQPQ 128
Query: 1130 EITP 1133
P
Sbjct: 127 TTNP 116
>BP075943
Length = 547
Score = 78.6 bits (192), Expect = 8e-15
Identities = 46/157 (29%), Positives = 79/157 (50%), Gaps = 1/157 (0%)
Frame = -3
Query: 1400 IYVDDIIFGSSNASLCKEFSKLMQDEFEMSMMGELKFFMGIQINQSKEGVYVHQTKYTKE 1459
+YVDD++ ++ + + D+F + +GE K+F+G++I +S G+ ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1460 LLKKFKLEDCKMMNTPMHPTCTLSKEDTGT-VVDQKLYRGMIGSLLYLTASRPDILFSVC 1518
L+ + +TGT + D YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAVN 156
Query: 1519 LCARFQSDPRESHLTAVKRIFRYLKGTTNLGLLYRKS 1555
++F S P + H + +YL G+ GL Y S
Sbjct: 155 QLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPAS 45
>BP057233
Length = 473
Score = 69.7 bits (169), Expect = 4e-12
Identities = 32/73 (43%), Positives = 48/73 (64%)
Frame = -2
Query: 1638 IYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLS 1697
++CDN +A L+ NP+LH+R+KHIEI H+IRD V + + + ++ T Q AD TKPLS
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1698 VERFDFIKKNLNM 1710
RF ++ L +
Sbjct: 265 HTRFSQLRDKLGV 227
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 69.3 bits (168), Expect = 5e-12
Identities = 33/91 (36%), Positives = 53/91 (57%), Gaps = 1/91 (1%)
Frame = +1
Query: 1621 WMKHQLEDYQINANSIP-IYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGILDI 1679
W+ + L+D ++ +YCDN +A ++ NP+ H R KHIEI H +R+ +QKG++ +
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1680 QFIDTEHQWADIFTKPLSVERFDFIKKNLNM 1710
I + ADI+TK LS + F I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP063694
Length = 511
Score = 65.1 bits (157), Expect = 9e-11
Identities = 40/122 (32%), Positives = 62/122 (50%), Gaps = 3/122 (2%)
Frame = -2
Query: 799 VWHKRLGHANWRLISKISK---LQLVKGLPNIDYHSDALCGACQKGKIVKSSFKSKDIVS 855
+WH RLGH N+ +I ++ K L + LP C +CQ K + F +
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNN-KR 199
Query: 856 TSRPLELLHIDLFGPVNTASLYGSKYGLVIVDDYSRWTWVKFIKSKDYACEVFSSFCIQI 915
S L+L+H DL GP AS+ G Y ++ VDD+SR+TW +K K +V F + +
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 916 QS 917
++
Sbjct: 18 EN 13
>AV766665
Length = 601
Score = 62.8 bits (151), Expect = 4e-10
Identities = 34/76 (44%), Positives = 44/76 (57%)
Frame = +3
Query: 1533 TAVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLISW 1592
T RIF YLK + G L++K + GF ADY G ++R ST G FL NL++W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1593 ASKRQATIAMSTAEAE 1608
SK+Q IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>BP043850
Length = 515
Score = 62.4 bits (150), Expect = 6e-10
Identities = 27/93 (29%), Positives = 55/93 (59%), Gaps = 1/93 (1%)
Frame = -1
Query: 1617 TQLLWMKHQLEDYQ-INANSIPIYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKG 1675
++++W++ L + + + P++ DNT+AI ++ NP+ H +HIE+ H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1676 ILDIQFIDTEHQWADIFTKPLSVERFDFIKKNL 1708
++ + + T Q ADI TK L+ +R +F+ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 46.6 bits (109), Expect(2) = 1e-09
Identities = 23/51 (45%), Positives = 33/51 (64%)
Frame = +3
Query: 1660 HIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLSVERFDFIKKNLNM 1710
+IE K+HF+RD V KG + ++ T+ Q ADI TK L ERF ++ LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 34.3 bits (77), Expect(2) = 1e-09
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 1641 DNTAAICLSKNPILHSRAKHIE 1662
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 60.1 bits (144), Expect = 3e-09
Identities = 34/145 (23%), Positives = 73/145 (49%), Gaps = 9/145 (6%)
Frame = +2
Query: 1370 NFLIKNDFERGQVDTTLFRKTLK-KDILIVQIYVDDIIFGSSNASLCKEFSKLMQDEFEM 1428
+F++ + R D + K D +I+ +YVDD++ N +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1429 SMMGELKFFMGIQINQSKEG--VYVHQTKYTKELLKKFKLEDCKMMNTPMHPTCTLSKED 1486
+G +G+QI++ ++ +++ Q Y +++L++F ++DC ++TP+ LS
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1487 TGTVVDQKL------YRGMIGSLLY 1505
+ +++ Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 58.9 bits (141), Expect = 6e-09
Identities = 35/116 (30%), Positives = 63/116 (54%), Gaps = 3/116 (2%)
Frame = +3
Query: 1590 ISWASKRQATIAMSTAEAEYISAASCCTQLLWMKHQLEDYQINANSIPIYC---DNTAAI 1646
++W S+ Q +A+STAEAE+I+A C +LLWMK+ L++ +++ PI C A
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSH--PILCCIVITKALF 200
Query: 1647 CLSKNPILHSRAKHIEIKHHFIRDYVQKGILDIQFIDTEHQWADIFTKPLSVERFD 1702
L++ + + +RD + +L+++ I T+ AD+ TK L E+ +
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREKLE 368
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 58.2 bits (139), Expect = 1e-08
Identities = 44/148 (29%), Positives = 68/148 (45%), Gaps = 6/148 (4%)
Frame = -1
Query: 1453 QTKYTKELLKKFKLEDCKMMNTPM-HPTCTLSKEDTGTVVDQKLYRGMIGSLLYLTASRP 1511
Q Y ++LKKFK+ + K ++T + + + G VD Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1512 DILFSVCLCARFQSDPRESHLTAVKRIFRYLKGTTNLGLLYR-----KSLDYKLIGFCDA 1566
DI+ V L +RF +P + H +R RY+KGT G+ SLD +++
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*NSLDTQIV----- 398
Query: 1567 DYAGDRIERKSTSGNCQFLGENLISWAS 1594
+ G LG ISW+S
Sbjct: 397 ----------TRPGYAFHLGRGAISWSS 344
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 57.4 bits (137), Expect = 2e-08
Identities = 30/81 (37%), Positives = 48/81 (59%), Gaps = 1/81 (1%)
Frame = +3
Query: 1618 QLLWMKHQLEDYQI-NANSIPIYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGI 1676
+ LW+ + L D +I + + IYCDN +A+ L+ N + H R ++IEI H + V GI
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1677 LDIQFIDTEHQWADIFTKPLS 1697
L + + + Q AD+FTK +S
Sbjct: 201 LHLLHVPSSDQVADVFTKTIS 263
>AV427921
Length = 284
Score = 55.8 bits (133), Expect = 5e-08
Identities = 31/79 (39%), Positives = 42/79 (52%)
Frame = +1
Query: 957 TPQQNRVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYEL 1016
TPQQN V ERKNRT+ M R+++ + L K F EAV S +I NR + E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 1017 FKGRRPNISYFHQFGCTCY 1035
+ GR+P + +F C Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>AU088980
Length = 360
Score = 55.1 bits (131), Expect = 9e-08
Identities = 29/112 (25%), Positives = 52/112 (45%)
Frame = +2
Query: 961 NRVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYELFKGR 1020
N +VERK+R + + R ++ + L K+ W AV + Y+ N + + + Y+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 1021 RPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNSET 1072
P + FG CY +K D +A++ +FLG+ + Y V + T
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPT 337
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 55.1 bits (131), Expect = 9e-08
Identities = 29/94 (30%), Positives = 51/94 (53%), Gaps = 1/94 (1%)
Frame = +1
Query: 1618 QLLWMKHQLEDYQINANSIPI-YCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGI 1676
+L W+ + L+D ++ S + YCD+ +A ++ N + H R KH++I H +R+ +Q +
Sbjct: 4 EL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKL 183
Query: 1677 LDIQFIDTEHQWADIFTKPLSVERFDFIKKNLNM 1710
+ I + Q ADI TKPL F + L +
Sbjct: 184 FHLLPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>AV424544
Length = 276
Score = 48.9 bits (115), Expect = 7e-06
Identities = 23/90 (25%), Positives = 50/90 (55%)
Frame = +3
Query: 1367 RLSNFLIKNDFERGQVDTTLFRKTLKKDILIVQIYVDDIIFGSSNASLCKEFSKLMQDEF 1426
+LS++L + + D +LF K ++ +YVDD+I ++ + + + +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 1427 EMSMMGELKFFMGIQINQSKEGVYVHQTKY 1456
+ +G LK+F+G+++ +S G+++ Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 517
Score = 35.0 bits (79), Expect(2) = 2e-05
Identities = 24/56 (42%), Positives = 34/56 (59%), Gaps = 2/56 (3%)
Frame = -2
Query: 1641 DNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGILDI--QFIDTEHQWADIFTK 1694
DN AA+ + K RAKHIEI HF+ D ++ LDI +F+++ Q D+FTK
Sbjct: 354 DNKAALHMPKI*F-SMRAKHIEIDFHFL*D--RRLYLDISTRFVNSNDQLTDVFTK 196
Score = 31.6 bits (70), Expect(2) = 2e-05
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -1
Query: 1585 LGENLISWASKRQATIAMSTAEAEY 1609
L +NLISW SK +A S AEAE+
Sbjct: 517 LSDNLISWKSKETIIVARSRAEAEF 443
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.131 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,950,509
Number of Sequences: 28460
Number of extensions: 353747
Number of successful extensions: 1600
Number of sequences better than 10.0: 92
Number of HSP's better than 10.0 without gapping: 1563
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1590
length of query: 1715
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1612
effective length of database: 1,966,220
effective search space: 3169546640
effective search space used: 3169546640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146757.2