Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146757.12 + phase: 0 /partial
         (192 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC16848 homologue to UP|BAD08451 (BAD08451) Glucose-6-phosphate ...    27  3.0
TC17020 weakly similar to UP|Q8LDT8 (Q8LDT8) GDSL-motif lipase/h...    26  5.1
TC12527 homologue to UP|Q9SWV9 (Q9SWV9) Ethylene-responsive RNA ...    26  5.1
AV418747                                                               25  6.6
BP065779                                                               25  8.6
TC10147 weakly similar to GB|AAO39907.1|28372850|BT003679 At1g67...    25  8.6
TC8843                                                                 25  8.6

>TC16848 homologue to UP|BAD08451 (BAD08451) Glucose-6-phosphate isomerase,
           partial (7%)
          Length = 723

 Score = 26.6 bits (57), Expect = 3.0
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = +1

Query: 166 NDVETFCQHLVQMEEEVIKKVSA 188
           ND+  +C H +QM E ++ K +A
Sbjct: 10  NDLPNYCSHWLQMTEHLLPKAAA 78


>TC17020 weakly similar to UP|Q8LDT8 (Q8LDT8) GDSL-motif
           lipase/hydrolase-like protein, partial (8%)
          Length = 604

 Score = 25.8 bits (55), Expect = 5.1
 Identities = 14/36 (38%), Positives = 20/36 (54%)
 Frame = +3

Query: 114 FVYMNTNAKDAYVTSVDRKGFDVLAKVTGPVSKDGV 149
           +V++NT A    +     +GF V  KV  P +KDGV
Sbjct: 24  YVFINTTA----IIHDKSQGFTVTEKVCCPTNKDGV 119


>TC12527 homologue to UP|Q9SWV9 (Q9SWV9) Ethylene-responsive RNA helicase,
           partial (26%)
          Length = 690

 Score = 25.8 bits (55), Expect = 5.1
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = +2

Query: 148 GVGQYQWKELRFMFEQEANDVETFCQHLVQMEEEVIKKVS 187
           G+G+   KELR+ F   AN    F + L+ + EE  ++VS
Sbjct: 239 GLGELVQKELRYTFFTAAN--ARFAKELITILEEAGQRVS 352


>AV418747 
          Length = 386

 Score = 25.4 bits (54), Expect = 6.6
 Identities = 11/31 (35%), Positives = 17/31 (54%)
 Frame = +1

Query: 160 MFEQEANDVETFCQHLVQMEEEVIKKVSASS 190
           +F    ND+E   QHL + E  V+ K++  S
Sbjct: 274 IFRSNGNDLEKRLQHLSKAEASVMNKIANRS 366


>BP065779 
          Length = 424

 Score = 25.0 bits (53), Expect = 8.6
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = -2

Query: 40  SDNLQKLLEVTEKYSVYRFKTRSCTFI 66
           SDN+Q L++  + +    F+TRSC FI
Sbjct: 144 SDNVQ*LVKANQSF--IGFRTRSCIFI 70


>TC10147 weakly similar to GB|AAO39907.1|28372850|BT003679 At1g67250
           {Arabidopsis thaliana;}, partial (96%)
          Length = 622

 Score = 25.0 bits (53), Expect = 8.6
 Identities = 13/43 (30%), Positives = 19/43 (43%)
 Frame = +1

Query: 150 GQYQWKELRFMFEQEANDVETFCQHLVQMEEEVIKKVSASSGL 192
           G+ +WK+L+  F  ++    T C  L  ME      V   S L
Sbjct: 55  GRRRWKKLQRPFHTKSEVSTTICSALESMESRATSLVLTLSNL 183


>TC8843 
          Length = 710

 Score = 25.0 bits (53), Expect = 8.6
 Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
 Frame = -2

Query: 18  ATRKFSYTVSGVLSSGGSSTSRSDNLQKLLEVTEKY-SVYRFKTRSCTFIDGHGGT 72
           ATR FS+ +S V +      S+ DNL K  E  +K   V + +  SC++   HG T
Sbjct: 391 ATRGFSHYISVVDTLVSIKKSQDDNLLKQQENKQKAGKVDKSRY*SCSWGISHG*T 224


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.316    0.131    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,653,838
Number of Sequences: 28460
Number of extensions: 27646
Number of successful extensions: 154
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of query: 192
length of database: 4,897,600
effective HSP length: 85
effective length of query: 107
effective length of database: 2,478,500
effective search space: 265199500
effective search space used: 265199500
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)


Medicago: description of AC146757.12