BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.8 - phase: 0 /pseudo
(469 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP046665 143 5e-35
TC8084 similar to UP|PIF1_YEAST (P07271) DNA repair and recombin... 127 3e-30
TC15490 weakly similar to UP|Q9LTU4 (Q9LTU4) Helicase-like prote... 94 1e-25
BP085968 75 6e-23
TC8086 similar to UP|DMC1_HUMAN (Q14565) Meiotic recombination p... 75 4e-15
BP065488 58 3e-09
AU089042 56 1e-08
TC11129 weakly similar to UP|PDA6_MEDSA (P38661) Probable protei... 35 0.020
TC14212 similar to UP|Q9FN61 (Q9FN61) Gb|AAF07369.1, partial (10%) 32 0.22
TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete 28 3.2
TC9303 28 4.2
BP068480 27 5.5
TC8586 UP|RPOA_LOTJA (Q9BBQ4) DNA-directed RNA polymerase alpha ... 27 7.1
NP459554 RNA polymerase alpha subunit [Lotus japonicus] 27 7.1
BP064797 27 7.1
BP067424 27 9.3
BP033261 27 9.3
TC13256 similar to UP|Q9XI49 (Q9XI49) F9L1.14 protein, partial (... 27 9.3
AV418236 27 9.3
>BP046665
Length = 524
Score = 143 bits (361), Expect = 5e-35
Identities = 71/120 (59%), Positives = 87/120 (72%)
Frame = -1
Query: 335 YGLPNHSIILKVGTPIMLLRNLDQAEGLCNGTRLTITRLANHVIEVKIITEKNIGNQVYI 394
+G+PNH I LK G PIMLLRN+ QA G CNGTRL + L +VI+ +IT NIG+ ++I
Sbjct: 512 FGIPNHKITLKEGAPIMLLRNIYQAVGFCNGTRLIVADLGTNVIKATVIT*TNIGDDIFI 333
Query: 395 PGISMSPSKLPWPFKLVRRQFSIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVAL 454
P + M PS +PFK RRQF I + A+TINKSQGQSL HVGLYL + VF+H QLYVAL
Sbjct: 332 PRMDMVPSDSGYPFKFERRQFPISLCSAMTINKSQGQSLSHVGLYLSRHVFTHGQLYVAL 153
>TC8084 similar to UP|PIF1_YEAST (P07271) DNA repair and recombination
protein PIF1, mitochondrial precursor, partial (4%)
Length = 560
Score = 127 bits (320), Expect = 3e-30
Identities = 63/114 (55%), Positives = 82/114 (71%)
Frame = +1
Query: 356 LDQAEGLCNGTRLTITRLANHVIEVKIITEKNIGNQVYIPGISMSPSKLPWPFKLVRRQF 415
+ + E LCNGTRL + L +VI+ +IT NIG+ ++IP + M PS +PFK RR F
Sbjct: 43 IQKFENLCNGTRLIVVDLGTYVIKATVITGTNIGDDIFIPRLDMVPSDSGYPFKFERR*F 222
Query: 416 SIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKILIHD 469
I + FA+TINKSQGQSL HV LYL + VF+H QLYVALSRV+S +GLK+L+ D
Sbjct: 223 PISLCFAMTINKSQGQSLSHVSLYLSRPVFTHGQLYVALSRVRSRKGLKLLVLD 384
>TC15490 weakly similar to UP|Q9LTU4 (Q9LTU4) Helicase-like protein, partial
(8%)
Length = 634
Score = 93.6 bits (231), Expect(2) = 1e-25
Identities = 49/92 (53%), Positives = 65/92 (70%)
Frame = +2
Query: 378 IEVKIITEKNIGNQVYIPGISMSPSKLPWPFKLVRRQFSIVVSFAITINKSQGQSLDHVG 437
I+V +IT +IG+ + IP + M PS +PFK RRQ I + FA+TINKSQG+SL HVG
Sbjct: 137 IQVTVITGTHIGDDISIPRMDMVPSDSSYPFKFERRQSPISLCFAMTINKSQGRSLSHVG 316
Query: 438 LYLPKDVFSHEQLYVALSRVKSNEGLKILIHD 469
LYL + V +H LYVAL RV+S + LK+L+ D
Sbjct: 317 LYLSRPVSTHG*LYVALPRVRSRK*LKLLVLD 412
Score = 39.7 bits (91), Expect(2) = 1e-25
Identities = 19/29 (65%), Positives = 21/29 (71%)
Frame = +1
Query: 345 KVGTPIMLLRNLDQAEGLCNGTRLTITRL 373
K G IMLLRN+ QA GLCNGTRL + L
Sbjct: 43 KEGALIMLLRNIVQASGLCNGTRLIVVDL 129
>BP085968
Length = 341
Score = 75.5 bits (184), Expect(2) = 6e-23
Identities = 33/66 (50%), Positives = 49/66 (74%)
Frame = +2
Query: 14 EQKNIYNRIMEVVTNEKGGVFFLYGYGGIGKTYMWNILAASLRSRKDIVFNVATSGIVAL 73
+Q +Y +IM V ++ GG +FLYG+GG GKT++WN ++ LRS+ +V NVA+SGI +
Sbjct: 17 KQSKVYKQIMSSVWSDDGGFYFLYGFGGSGKTFVWNTWSSGLRSQGLMVLNVASSGIASW 196
Query: 74 LLPGGR 79
LLPGG+
Sbjct: 197LLPGGK 214
Score = 48.9 bits (115), Expect(2) = 6e-23
Identities = 22/43 (51%), Positives = 30/43 (69%)
Frame = +3
Query: 79 RTVHSRFKIPVPTLQSSICDIEKGTELAELLKQTKLIIWDEAP 121
RT HSRF I + S C+I++G++ AELL++ IIWDEAP
Sbjct: 213 RTAHSRFSIHISINDISTCNIKQGSQKAELLQKASSIIWDEAP 341
>TC8086 similar to UP|DMC1_HUMAN (Q14565) Meiotic recombination protein
DMC1/LIM15 homolog, partial (12%)
Length = 628
Score = 75.1 bits (183), Expect(2) = 4e-15
Identities = 36/75 (48%), Positives = 50/75 (66%)
Frame = +1
Query: 356 LDQAEGLCNGTRLTITRLANHVIEVKIITEKNIGNQVYIPGISMSPSKLPWPFKLVRRQF 415
+ + E LC+GTRL + L +VI+ +IT NIG+ ++IP + M PS +PFK RR F
Sbjct: 151 IQKFENLCHGTRLIVVDLGTYVIKATVITGTNIGDDIFIPRLDMVPSDSGYPFKFERR*F 330
Query: 416 SIVVSFAITINKSQG 430
I + FA+TINKSQG
Sbjct: 331 PISLCFAMTINKSQG 375
Score = 22.7 bits (47), Expect(2) = 4e-15
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = +3
Query: 455 SRVKSNEGLKILIHD 469
SRV+S +GLK+L+ D
Sbjct: 369 SRVRSRKGLKLLVLD 413
>BP065488
Length = 439
Score = 58.2 bits (139), Expect = 3e-09
Identities = 37/85 (43%), Positives = 51/85 (59%), Gaps = 2/85 (2%)
Frame = -1
Query: 385 EKNIGNQVYIPGISMSPSKLPWPFKLVRRQFSIVVSFAITINKSQGQ-SLDHVGLYLPKD 443
E G+ ++IP ++M PS P K R QF I + FA+TINKSQ V L
Sbjct: 391 EPTXGDDIFIPRMNMVPSVSGDPLKFERCQFPISLCFAMTINKSQXSVHYPTVALISFLA 212
Query: 444 VFSH-EQLYVALSRVKSNEGLKILI 467
+SH ++LYVALS V+S +GLK+L+
Sbjct: 211 CYSHMDKLYVALSGVRSRKGLKLLV 137
>AU089042
Length = 191
Score = 55.8 bits (133), Expect = 1e-08
Identities = 27/60 (45%), Positives = 39/60 (65%)
Frame = +3
Query: 343 ILKVGTPIMLLRNLDQAEGLCNGTRLTITRLANHVIEVKIITEKNIGNQVYIPGISMSPS 402
+LK G P+ML+ NL + GLCNGTRL + L +VI I++ +IG VYI +++ PS
Sbjct: 6 VLKXGVPVMLMXNLXISTGLCNGTRLIVDYLGPNVIGATILSGTHIGXVVYISMMNLXPS 185
>TC11129 weakly similar to UP|PDA6_MEDSA (P38661) Probable protein disulfide
isomerase A6 precursor (P5) , partial (7%)
Length = 600
Score = 35.4 bits (80), Expect = 0.020
Identities = 14/35 (40%), Positives = 24/35 (68%)
Frame = +2
Query: 282 TIQVVDDINNYVLNLIPGEEKEYFSSDSIDRSKED 316
T++ V+ +N ++L+L+PG EY SSD+ + ED
Sbjct: 461 TLESVEKVNEFMLDLLPGNTTEYLSSDTTCKYDED 565
>TC14212 similar to UP|Q9FN61 (Q9FN61) Gb|AAF07369.1, partial (10%)
Length = 613
Score = 32.0 bits (71), Expect = 0.22
Identities = 15/18 (83%), Positives = 17/18 (94%)
Frame = +2
Query: 450 LYVALSRVKSNEGLKILI 467
LYVA+SRVKS +GLKILI
Sbjct: 2 LYVAVSRVKSKDGLKILI 55
>TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete
Length = 1288
Score = 28.1 bits (61), Expect = 3.2
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +3
Query: 300 EEKEYFSSDSIDRSKEDGNDAFEQ 323
E+KEYF D +E GN+ F+Q
Sbjct: 501 EQKEYFDPKLADEEREKGNEFFKQ 572
>TC9303
Length = 609
Score = 27.7 bits (60), Expect = 4.2
Identities = 19/94 (20%), Positives = 44/94 (46%), Gaps = 1/94 (1%)
Frame = +3
Query: 237 IEISNELLISNFTDP-IEAIVQSTYSNLLENFQDGDFLRNRAILASTIQVVDDINNYVLN 295
+E++++ ++ +P I + + + LL+N + + ++V ++NY+ N
Sbjct: 135 VELTDDDTVTGSEEPEIHSGIAFDFIGLLQNLK-----------LTVAEMVSRVSNYLQN 281
Query: 296 LIPGEEKEYFSSDSIDRSKEDGNDAFEQVTPEFL 329
+ P EKE S D+ +K + D + F+
Sbjct: 282 IFPTGEKEKSSGDADGNTKANVMDNMNVMGGSFM 383
>BP068480
Length = 560
Score = 27.3 bits (59), Expect = 5.5
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = -3
Query: 157 YLEVILDKYYQ*CQEEIDLISSILQ*CLYIWNHCQVLKLTKNMRL 201
Y+E I +KY CQ +++ ++L L +++ CQ +K N+ L
Sbjct: 234 YIEGIGNKYIWYCQSLFEIVWTVLWILL*LFSLCQTVKCICNITL 100
>TC8586 UP|RPOA_LOTJA (Q9BBQ4) DNA-directed RNA polymerase alpha chain
(PEP) (Plastid-encoded RNA polymerase alpha subunit)
(RNA polymerase alpha subunit) , partial (79%)
Length = 856
Score = 26.9 bits (58), Expect = 7.1
Identities = 18/72 (25%), Positives = 34/72 (47%), Gaps = 7/72 (9%)
Frame = +2
Query: 239 ISNELLISNFTDPIEAIV-------QSTYSNLLENFQDGDFLRNRAILASTIQVVDDINN 291
+ N I+N T+PI+ + + N L+NFQDG + + + V + N+
Sbjct: 455 VDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSY-----NIDAIFMPVRNANH 619
Query: 292 YVLNLIPGEEKE 303
+ + + G EK+
Sbjct: 620 SIHSYVNGNEKQ 655
>NP459554 RNA polymerase alpha subunit [Lotus japonicus]
Length = 1002
Score = 26.9 bits (58), Expect = 7.1
Identities = 18/72 (25%), Positives = 34/72 (47%), Gaps = 7/72 (9%)
Frame = +1
Query: 239 ISNELLISNFTDPIEAIV-------QSTYSNLLENFQDGDFLRNRAILASTIQVVDDINN 291
+ N I+N T+PI+ + + N L+NFQDG + + + V + N+
Sbjct: 388 VDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSY-----NIDAIFMPVRNANH 552
Query: 292 YVLNLIPGEEKE 303
+ + + G EK+
Sbjct: 553 SIHSYVNGNEKQ 588
>BP064797
Length = 425
Score = 26.9 bits (58), Expect = 7.1
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -2
Query: 359 AEGLCNGTRLTITRLANHVIEVKIITEKNIGNQVYIPGISMSPSKLPWPFKLVRRQFSIV 418
A + + R + R ++H+I + I Q+ PG S S S PF RR F I+
Sbjct: 367 AVSIFSQARFMLKRFSSHMIIINSIRRII---QIRFPGASSSLSSSHKPFNTTRRXFVII 197
>BP067424
Length = 473
Score = 26.6 bits (57), Expect = 9.3
Identities = 8/13 (61%), Positives = 12/13 (91%)
Frame = +3
Query: 401 PSKLPWPFKLVRR 413
P +LPWP++L+RR
Sbjct: 375 PHRLPWPYRLLRR 413
>BP033261
Length = 528
Score = 26.6 bits (57), Expect = 9.3
Identities = 15/60 (25%), Positives = 28/60 (46%), Gaps = 5/60 (8%)
Frame = +1
Query: 363 CNGTRLTITRLANHVIEVKIITEKNIGNQVYIPGISMSP-----SKLPWPFKLVRRQFSI 417
CN ++T++ ++ V+ + +G + PG+S P + PW F V FS+
Sbjct: 241 CNANQITVSAPSSLVLSFPESDLEKLGKLLATPGVSAQPGCIE*NPTPW-FSYVSAHFSV 417
>TC13256 similar to UP|Q9XI49 (Q9XI49) F9L1.14 protein, partial (22%)
Length = 552
Score = 26.6 bits (57), Expect = 9.3
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 203 NDQAQSSENTERFSE*ILKVGDGKLGEENDGYNDIE 238
ND R S ++K+ DG+L E+ D ND++
Sbjct: 226 NDSDDQPPAKRRLSSAVVKLEDGELPEDADAGNDVK 333
>AV418236
Length = 411
Score = 26.6 bits (57), Expect = 9.3
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = +3
Query: 395 PGISMSPSKLPWPFKLVRRQFSIVVSFAITINKSQGQSLDHVG 437
P ISM S PW + F++ +SF I + +SLD G
Sbjct: 42 PAISMEFSPPPW----FQPSFAVAISFLIAFRAHRKKSLDTSG 158
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.144 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,931,001
Number of Sequences: 28460
Number of extensions: 85242
Number of successful extensions: 506
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 503
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 469
length of database: 4,897,600
effective HSP length: 94
effective length of query: 375
effective length of database: 2,222,360
effective search space: 833385000
effective search space used: 833385000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146756.8