BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.3 - phase: 0
(138 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8048 homologue to UP|PSA6_SOYBN (O48551) Proteasome subunit al... 266 9e-73
TC14473 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 sub... 77 1e-15
TC12945 similar to UP|PSA2_ARATH (O23708) Proteasome subunit alp... 72 4e-14
TC8157 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit al... 70 2e-13
TC10276 homologue to UP|PSA7_CICAR (Q9SXU1) Proteasome subunit a... 60 2e-10
TC18392 homologue to UP|PSA2_ORYSA (Q9LSU2) Proteasome subunit a... 58 7e-10
TC14699 homologue to UP|PSA5_ORYSA (Q9LSU1) Proteasome subunit a... 50 1e-07
TC14662 similar to UP|PSA3_SPIOL (O24362) Proteasome subunit alp... 40 2e-04
TC16454 similar to UP|O81152 (O81152) 20S proteasome beta subuni... 34 0.008
BP032681 30 0.20
TC12551 weakly similar to UP|Q9LUV5 (Q9LUV5) Genomic DNA, chromo... 25 3.7
TC16001 25 4.8
BP048060 24 8.2
TC15480 similar to UP|V712_ARATH (Q9SIQ9) Vesicle-associated mem... 24 8.2
>TC8048 homologue to UP|PSA6_SOYBN (O48551) Proteasome subunit alpha type 6
(20S proteasome alpha subunit A) (20S proteasome
subunit alpha-1) (Proteasome iota subunit) , complete
Length = 1060
Score = 266 bits (680), Expect = 9e-73
Identities = 131/138 (94%), Positives = 135/138 (96%)
Frame = +3
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENG QLYKCDPAGHYFGHKATSA
Sbjct: 465 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGAQLYKCDPAGHYFGHKATSA 644
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFR 120
G KDQEAINFLEKKMKNDPSFTY+ETVQTAI+ALQSVLQEDFKATEIEVGVV+KD PEFR
Sbjct: 645 GLKDQEAINFLEKKMKNDPSFTYEETVQTAISALQSVLQEDFKATEIEVGVVRKDNPEFR 824
Query: 121 VLSTEEIDEHLTAISERD 138
VLST+EIDEHLTAISERD
Sbjct: 825 VLSTDEIDEHLTAISERD 878
>TC14473 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 subunit,
partial (86%)
Length = 1302
Score = 77.0 bits (188), Expect = 1e-15
Identities = 46/134 (34%), Positives = 80/134 (59%), Gaps = 1/134 (0%)
Frame = +3
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L +ADK+QV TQ ++ RP GV +V G+D E+G LY P+G+YF ++A +
Sbjct: 444 LPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVGGLD-ESGAHLYYNCPSGNYFEYQAFAI 620
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQ-EDFKATEIEVGVVQKDKPEF 119
GS+ Q A +LE++ +N S + ++ ++ A+ A + LQ E +++ + VV +P F
Sbjct: 621 GSRSQAAKTYLERRFENFVSSSREDLIKDALIATRESLQGEKLRSSVCTIAVVGVGEP-F 797
Query: 120 RVLSTEEIDEHLTA 133
+L E + + + A
Sbjct: 798 HILDQETVQQMIDA 839
>TC12945 similar to UP|PSA2_ARATH (O23708) Proteasome subunit alpha type 2
(20S proteasome alpha subunit B) , partial (93%)
Length = 744
Score = 71.6 bits (174), Expect = 4e-14
Identities = 40/116 (34%), Positives = 58/116 (49%)
Frame = +3
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L + +A Q +TQ +RP GV ++ G DD NGPQLY+ P+G YF KA++
Sbjct: 369 IPVTQLVREVAAVMQEFTQSGGVRPFGVSLLIAGFDD-NGPQLYQVXPSGSYFSWKASAM 545
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDK 116
G A FL KK + D+ TA+ L + IE+G++ K
Sbjct: 546 GKNVSNAKTFLXKKYTD--XMXLDDAXHTALLTLXEXFEGXISXKNIEIGIIGXXK 707
>TC8157 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit alpha type 4
(20S proteasome alpha subunit C) (20S proteasome
subunit alpha-3) (Proteasome 27 kDa subunit) , complete
Length = 1080
Score = 69.7 bits (169), Expect = 2e-13
Identities = 45/135 (33%), Positives = 69/135 (50%), Gaps = 4/135 (2%)
Frame = +1
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
MPV+ L + + D Q YTQ +RP GV + G D G QLY DP+G+Y G KA +
Sbjct: 445 MPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNFGFQLYMSDPSGNYGGWKAGAI 624
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATE----IEVGVVQKDK 116
G+ +Q A + L++ K+D T +E VQ A+ L + ++ EV + K
Sbjct: 625 GANNQAAQSILKQDYKDD--ITREEAVQLALKVLSKTMDSTSLTSDKLELAEVYLSPSGK 798
Query: 117 PEFRVLSTEEIDEHL 131
++ V S E + + L
Sbjct: 799 VKYEVCSPESLTKLL 843
>TC10276 homologue to UP|PSA7_CICAR (Q9SXU1) Proteasome subunit alpha type 7
(20S proteasome alpha subunit D) (20S proteasome
subunit alpha-4) , partial (82%)
Length = 688
Score = 59.7 bits (143), Expect = 2e-10
Identities = 35/102 (34%), Positives = 51/102 (49%), Gaps = 1/102 (0%)
Frame = +2
Query: 3 VDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENG-PQLYKCDPAGHYFGHKATSAG 61
V+ + ++IA Q YTQ +RP G+ +++G D P LY+ DP+G + KA + G
Sbjct: 392 VEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYTATPSLYQTDPSGTFSAWKANATG 571
Query: 62 SKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFK 103
FLEK K ETV+ AI AL V++ K
Sbjct: 572 RNSNSIREFLEKNFKETSG---QETVKLAIRALLEVVESGGK 688
>TC18392 homologue to UP|PSA2_ORYSA (Q9LSU2) Proteasome subunit alpha type 2
(20S proteasome alpha subunit B) (20S proteasome
subunit alpha-2) , partial (71%)
Length = 548
Score = 57.8 bits (138), Expect = 7e-10
Identities = 29/61 (47%), Positives = 39/61 (63%)
Frame = +1
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L + +A Q +TQ +RP GV +V G DD NGPQLY+ DP+G YF KA++
Sbjct: 355 IPVTQLVREVAAVMQEFTQSGGVRPFGVSLLVAGFDD-NGPQLYQVDPSGSYFSWKASAM 531
Query: 61 G 61
G
Sbjct: 532 G 534
>TC14699 homologue to UP|PSA5_ORYSA (Q9LSU1) Proteasome subunit alpha type 5
(20S proteasome alpha subunit E) (20S proteasome
subunit alpha-5) , complete
Length = 1044
Score = 50.4 bits (119), Expect = 1e-07
Identities = 29/110 (26%), Positives = 58/110 (52%)
Frame = +1
Query: 19 QHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKND 78
+ + RP GV ++ G DENGP LY DP+G ++ A + GS + A + L+++ +
Sbjct: 463 EESMSRPFGVSLLIAG-HDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKE 639
Query: 79 PSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFRVLSTEEID 128
+ ET+ A++ L+ V++E +++ V P + + + E++
Sbjct: 640 LTLQEAETI--ALSILKQVMEEKVTPNNVDIAQV---SPTYHLYTPAEVE 774
>TC14662 similar to UP|PSA3_SPIOL (O24362) Proteasome subunit alpha type 3
(20S proteasome alpha subunit G) (20S proteasome subunit
alpha-7) (Proteasome component C8) , complete
Length = 1110
Score = 39.7 bits (91), Expect = 2e-04
Identities = 24/73 (32%), Positives = 38/73 (51%)
Frame = +2
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV LA +A + T + ++RP G ++LG D GPQLY +P+G + + +
Sbjct: 377 IPVKELADRVASYVHLCTLYWWLRPFGC-GVILGGYDRYGPQLYMVEPSGVSYRYFGAAI 553
Query: 61 GSKDQEAINFLEK 73
G Q A +EK
Sbjct: 554 GKGRQAAKTEIEK 592
>TC16454 similar to UP|O81152 (O81152) 20S proteasome beta subunit PBB1
(20S proteasome beta subunit, multicatalytic
endopeptidase) (At3g27430) , complete
Length = 1277
Score = 34.3 bits (77), Expect = 0.008
Identities = 26/91 (28%), Positives = 42/91 (45%), Gaps = 1/91 (1%)
Frame = +1
Query: 30 AMVLGIDDENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKNDPSFTYDETVQT 89
A+VLG D GP L+ P G + GS A++ E K K S T DE ++
Sbjct: 496 ALVLGGVDATGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFESKYKE--SLTRDEGIKI 669
Query: 90 AIAALQSVLQEDF-KATEIEVGVVQKDKPEF 119
+ A+ + + D + ++V V+ K E+
Sbjct: 670 VVEAICAGVFNDLGSGSNVDVCVITKGHKEY 762
>BP032681
Length = 392
Score = 29.6 bits (65), Expect = 0.20
Identities = 14/28 (50%), Positives = 21/28 (75%)
Frame = -2
Query: 104 ATEIEVGVVQKDKPEFRVLSTEEIDEHL 131
A IE+G++ DK +FRVL+ EID++L
Sbjct: 382 AKNIEIGIIGADK-KFRVLTPAEIDDYL 302
>TC12551 weakly similar to UP|Q9LUV5 (Q9LUV5) Genomic DNA, chromosome 3, P1
clone: MCB17, partial (66%)
Length = 568
Score = 25.4 bits (54), Expect = 3.7
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +2
Query: 28 VVAMVLGIDDENGPQLYKCD 47
V +LG DDEN P L K D
Sbjct: 248 VQPQILGKDDENNPNLVKVD 307
>TC16001
Length = 706
Score = 25.0 bits (53), Expect = 4.8
Identities = 9/26 (34%), Positives = 17/26 (64%)
Frame = +2
Query: 103 KATEIEVGVVQKDKPEFRVLSTEEID 128
KA ++ GV+ D P+F++ S E ++
Sbjct: 359 KAEKVAAGVIDYDVPDFKLPSKEAVE 436
>BP048060
Length = 479
Score = 24.3 bits (51), Expect = 8.2
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +1
Query: 9 WIADKSQVYTQHAYMRP 25
W D++Q +QHAY+ P
Sbjct: 250 WYEDETQCSSQHAYLLP 300
>TC15480 similar to UP|V712_ARATH (Q9SIQ9) Vesicle-associated membrane
protein 712 (AtVAMP712), partial (53%)
Length = 482
Score = 24.3 bits (51), Expect = 8.2
Identities = 8/19 (42%), Positives = 14/19 (73%)
Frame = -3
Query: 62 SKDQEAINFLEKKMKNDPS 80
S D+ +NFLEK+ ++ P+
Sbjct: 396 SPDESLVNFLEKRKRDSPA 340
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.132 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,810,148
Number of Sequences: 28460
Number of extensions: 18383
Number of successful extensions: 78
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 74
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of query: 138
length of database: 4,897,600
effective HSP length: 81
effective length of query: 57
effective length of database: 2,592,340
effective search space: 147763380
effective search space used: 147763380
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 50 (23.9 bits)
Medicago: description of AC146756.3