BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.1 - phase: 0
(371 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17948 similar to UP|Q9FZ13 (Q9FZ13) Tuber-specific and sucrose... 35 0.020
TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromos... 33 0.099
CB829198 33 0.099
TC14217 32 0.13
CN825310 31 0.38
BP037078 30 0.49
CN825403 30 0.64
BP049555 29 1.1
BI419295 29 1.4
BI420780 28 1.9
TC8520 homologue to UP|Q9FZ15 (Q9FZ15) Tuber-specific and sucros... 28 1.9
BG662133 28 2.4
BP064305 27 4.2
TC10412 homologue to UP|Q9FVE8 (Q9FVE8) Plasma membrane Ca2+-ATP... 27 4.2
TC9457 homologue to UP|Q9FZ15 (Q9FZ15) Tuber-specific and sucros... 27 4.2
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 27 4.2
TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%) 27 5.5
TC9431 weakly similar to UP|Q84UI1 (Q84UI1) Allergen len c 3.02 ... 27 7.1
TC8280 27 7.1
AV769448 27 7.1
>TC17948 similar to UP|Q9FZ13 (Q9FZ13) Tuber-specific and sucrose-responsive
element binding factor, partial (50%)
Length = 487
Score = 35.0 bits (79), Expect = 0.020
Identities = 15/54 (27%), Positives = 31/54 (56%), Gaps = 1/54 (1%)
Frame = +2
Query: 6 NHQNQNKNPNQDLQG-WGTWEELLLASAVDRHGFKDWDTIAMEVQSRTNRTSLL 58
N ++ N N + ++G W E+ +L V+RHG ++W I+ ++ R+ ++ L
Sbjct: 131 NPRSNNPNRPERIKGPWSAEEDRILTRLVERHGARNWSLISRYIKGRSGKSCRL 292
>TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromosome 3, P1
clone: MSJ11, partial (39%)
Length = 1403
Score = 32.7 bits (73), Expect = 0.099
Identities = 26/113 (23%), Positives = 51/113 (45%), Gaps = 7/113 (6%)
Frame = +3
Query: 202 NQSVNESNSTGSRFDALKTGEVDVKLGSGPGHGSGSVQVKSGLNEPD-QTGRKRKSVEEE 260
NQ ++ +T + D + + DVK + PG + + LN D + ++ V+++
Sbjct: 540 NQDEKKAEATDANQDEKRAEDADVKEPTHPGENTDGPNDEGKLNTADEELQASKEKVDDK 719
Query: 261 SNNGS-----YDNNEAKAVTCESVPPSEERKVEDDSVTRSE-GGGTRESSEVQ 307
GS D+ + E+ P +E+ K+E + + E G ES E++
Sbjct: 720 GAEGSDAVQNKDDGSGEPEKSEAAPTTEQ-KIEVEGENKEEHDRGIHESGEIK 875
>CB829198
Length = 562
Score = 32.7 bits (73), Expect = 0.099
Identities = 20/81 (24%), Positives = 39/81 (47%), Gaps = 5/81 (6%)
Frame = +3
Query: 244 LNEPDQTGRKRKSVEEESNNGSYDNNEAKAVTCESVPPSEER-KVEDDSVTRSEGGGTRE 302
+ E + K ++ EEE +G +NN+ K ++ER D+ V + +GGG +
Sbjct: 276 VEESQHSDSKEETEEEEDEDGDNENNKPKRAKAAKKKKNKERSNKSDNEVVKKKGGGFCK 455
Query: 303 ----SSEVQSSSSLSKTRRTQ 319
S ++Q + + + RT+
Sbjct: 456 LCSLSPQLQEFTGVPEMARTE 518
>TC14217
Length = 985
Score = 32.3 bits (72), Expect = 0.13
Identities = 30/162 (18%), Positives = 70/162 (42%), Gaps = 5/162 (3%)
Frame = +1
Query: 178 RRLDESTTNTDKLLPAAGEESDRDNQSVNESNSTGSRFDALKTGEVDVKLGSGPGHGSGS 237
R ++ T ++ A ++ ++ V + D K D K+
Sbjct: 112 REVEVETKKVEEQNEAEAATTETESVKVENVKNDTQAVDDKKEENADTKVD--------- 264
Query: 238 VQVKSGLNEPDQTGRKRKSVEEESNNGSYDNNEAKAVTCESVPPSEERKVEDDSVTRSEG 297
++ + ++EP + K +EES DN E + V E +E + ++ VT++
Sbjct: 265 -EISTAISEPVRETLASKFKDEESIETGVDNTEKEQV--EEPVKTEVQDTKESDVTKTSK 435
Query: 298 GGTRES----SEVQSSSSLSKTRRT-QRRRKEVSGEDAKMEN 334
+E+ ++ QS++ +SK +++ + +K ++G+ +N
Sbjct: 436 DLPKETPAKPAQKQSNNIISKVKQSLAKAKKAITGKSPSSKN 561
>CN825310
Length = 747
Score = 30.8 bits (68), Expect = 0.38
Identities = 23/88 (26%), Positives = 40/88 (45%), Gaps = 2/88 (2%)
Frame = +1
Query: 258 EEESNNGSYDNNEAKAVTCESVPPSEERKVEDDSVTRSEGGGTRESSEVQSSSSL--SKT 315
E E ++ Y +N + + E + ++ V +S + EG +ESS SSL S +
Sbjct: 481 ENEQSDQPYKDNALQIIVREQM---DDVHVSPESSQKKEGNQVKESSSQHQVSSLTQSTS 651
Query: 316 RRTQRRRKEVSGEDAKMENEDVAVVKSE 343
R++ RR + K + +KSE
Sbjct: 652 ARSEGRRSRSGKKAKKRHTRQKSQLKSE 735
>BP037078
Length = 529
Score = 30.4 bits (67), Expect = 0.49
Identities = 22/78 (28%), Positives = 41/78 (52%)
Frame = +1
Query: 105 LRKQRVAELRRDVQLSDVSILSLQLQVKKLEDEKAKENEEKVETEPDLAVSGEGRLPENE 164
L+K+ + + +V + LQ QV+ L+ E K+N+EK V+ E R+ E E
Sbjct: 316 LQKKGSLDAQEEVGKAHARAGELQKQVENLKRELEKQNKEK--------VNWETRVAELE 471
Query: 165 KTGGDIDEPVPAIRRLDE 182
K D++ + I++++E
Sbjct: 472 KKIHDLNSKLEDIQKVNE 525
>CN825403
Length = 688
Score = 30.0 bits (66), Expect = 0.64
Identities = 29/112 (25%), Positives = 50/112 (43%), Gaps = 9/112 (8%)
Frame = +3
Query: 227 LGSGPGHGSGSVQVKSGLNEPDQTGRKRKSVEEESNNGSYDNNEAKAVTCESVPPSEE-- 284
+GS SG+ S +P + +K S + E+++ + + + + VPP E+
Sbjct: 321 IGSSSRTSSGT----SNTGKPVKKAQKTLSSQLETDSSETSSVQDEPEVSKPVPPPEKVQ 488
Query: 285 -------RKVEDDSVTRSEGGGTRESSEVQSSSSLSKTRRTQRRRKEVSGED 329
E +VTR+EGG SSS +S TR RR+ + G++
Sbjct: 489 RGLHGEVENSESGNVTRTEGG---------SSSLVSNTR--SRRKPALRGQE 611
>BP049555
Length = 555
Score = 29.3 bits (64), Expect = 1.1
Identities = 18/55 (32%), Positives = 25/55 (44%), Gaps = 1/55 (1%)
Frame = +2
Query: 128 QLQVKKLEDEKAKEN-EEKVETEPDLAVSGEGRLPENEKTGGDIDEPVPAIRRLD 181
Q Q + +EK +E EEK E +P E PE EK + P P + +D
Sbjct: 248 QPQAEAKPEEKTEEKAEEKAEEKPAAEEKKEEEKPEEEKKEEEPKPPAPCVLYVD 412
>BI419295
Length = 578
Score = 28.9 bits (63), Expect = 1.4
Identities = 16/56 (28%), Positives = 24/56 (42%)
Frame = +2
Query: 218 LKTGEVDVKLGSGPGHGSGSVQVKSGLNEPDQTGRKRKSVEEESNNGSYDNNEAKA 273
LK G + +G+ P G K GL +P S++ +NN + AKA
Sbjct: 140 LKPGSLLENVGAQPAKGKEIATAKVGLEKPQGVNESGPSLDGSANNSNASAPAAKA 307
>BI420780
Length = 475
Score = 28.5 bits (62), Expect = 1.9
Identities = 10/39 (25%), Positives = 24/39 (60%)
Frame = +1
Query: 16 QDLQGWGTWEELLLASAVDRHGFKDWDTIAMEVQSRTNR 54
++ Q W + E+ LL + V ++G ++W+ ++ + + NR
Sbjct: 196 KERQRWSSEEDALLHAYVQQYGPREWNLVSQRMNTPLNR 312
>TC8520 homologue to UP|Q9FZ15 (Q9FZ15) Tuber-specific and
sucrose-responsive element binding factor, partial (36%)
Length = 1778
Score = 28.5 bits (62), Expect = 1.9
Identities = 11/38 (28%), Positives = 20/38 (51%)
Frame = +1
Query: 21 WGTWEELLLASAVDRHGFKDWDTIAMEVQSRTNRTSLL 58
W E+ L V+RHG ++W I+ + R+ ++ L
Sbjct: 193 WSPEEDEALQKLVERHGPRNWSLISKSIPGRSGKSCRL 306
>BG662133
Length = 428
Score = 28.1 bits (61), Expect = 2.4
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +3
Query: 243 GLNEPDQTGRKRKSVEEESNNGSYDNNEAKAVTCESVPPSE 283
G NEPD T E N +Y NN + V +S PPS+
Sbjct: 285 GANEPDAT---------EKNAETYSNNMIRRVMNDSGPPSQ 380
>BP064305
Length = 510
Score = 27.3 bits (59), Expect = 4.2
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = -3
Query: 216 DALKTGEVDVKLGSGPGHGSGSVQVKS 242
+AL G K GSG G GSV +KS
Sbjct: 82 EALHVGNRKAKFGSGDGSRGGSVYIKS 2
>TC10412 homologue to UP|Q9FVE8 (Q9FVE8) Plasma membrane Ca2+-ATPase,
partial (33%)
Length = 1061
Score = 27.3 bits (59), Expect = 4.2
Identities = 15/35 (42%), Positives = 23/35 (64%), Gaps = 2/35 (5%)
Frame = +2
Query: 188 DKLLPAAGEESDRDNQSVNESNSTGSRF--DALKT 220
DK+L + GE D +S+N NST ++F +AL+T
Sbjct: 152 DKVLNSNGEVVPLDEESINHLNSTINQFASEALRT 256
>TC9457 homologue to UP|Q9FZ15 (Q9FZ15) Tuber-specific and
sucrose-responsive element binding factor, partial
(43%)
Length = 860
Score = 27.3 bits (59), Expect = 4.2
Identities = 14/57 (24%), Positives = 25/57 (43%), Gaps = 7/57 (12%)
Frame = +3
Query: 9 NQNKNPNQDLQG-------WGTWEELLLASAVDRHGFKDWDTIAMEVQSRTNRTSLL 58
N N N N + W E+ L V++HG ++W I+ + R+ ++ L
Sbjct: 30 NTNTNTNTSITKMDRIKGPWSPEEDEALQRLVEKHGPRNWSLISKSIPGRSGKSCRL 200
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 27.3 bits (59), Expect = 4.2
Identities = 24/98 (24%), Positives = 39/98 (39%), Gaps = 11/98 (11%)
Frame = -2
Query: 229 SGPGHGSGSVQVKSGLNEPDQTGRK-RKSVEEESNNGSYDNNEAKAVTCES--------- 278
S P SGS + + + T R+ R SV + S++ S ++ + + + S
Sbjct: 327 SSPSSSSGSKSLDASSSSAAATVRRTRYSVPKSSSSSSSSSSSSSSSSSSSGSPPPPRPP 148
Query: 279 -VPPSEERKVEDDSVTRSEGGGTRESSEVQSSSSLSKT 315
PPS S + G + SS SSS S +
Sbjct: 147 STPPSSSSSSSSSSSSPPPGASSSSSSSPSSSSPSSSS 34
>TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%)
Length = 1196
Score = 26.9 bits (58), Expect = 5.5
Identities = 24/92 (26%), Positives = 35/92 (37%), Gaps = 12/92 (13%)
Frame = +2
Query: 252 RKRKSVEEESNNGSYDNNEAKAVTC------------ESVPPSEERKVEDDSVTRSEGGG 299
R SV + N D+ E KA + +S SE+ E +S + S G
Sbjct: 539 RVNVSVSYDLENNDVDDKEEKAKSSSKKAKRKHRSDSDSGSDSEDSVFETESGSSSVTGS 718
Query: 300 TRESSEVQSSSSLSKTRRTQRRRKEVSGEDAK 331
S SSS +RRRK+ + + K
Sbjct: 719 DYSSGSSSDSSSSDSEEERRRRRKKKTQKKKK 814
>TC9431 weakly similar to UP|Q84UI1 (Q84UI1) Allergen len c 3.02
(Fragment), partial (67%)
Length = 1328
Score = 26.6 bits (57), Expect = 7.1
Identities = 37/154 (24%), Positives = 61/154 (39%), Gaps = 13/154 (8%)
Frame = +2
Query: 231 PGHGSGSVQVKSGLNEPD------QTGRKRKSVEEESNNGSYDNNEAKAVTCESVPPSEE 284
PGH + Q G E D Q GR + E + D +E + V + +
Sbjct: 125 PGHRERARQ--EGEKEEDERQPWWQPGRGEEEGEWRGSRRLEDPDERERVRERTERAKKW 298
Query: 285 RKV--EDDSVTRSEGGGTRESSEVQSSSSLSKTRRTQRR-----RKEVSGEDAKMENEDV 337
R+ E D+ R + E SSSS +RR+QRR R S + +NE
Sbjct: 299 RRETEERDTPRRPHHRESEEEEGSSSSSSSESSRRSQRRNPFYFRSSSSRFQTRFQNEYG 478
Query: 338 AVVKSEPLFGVLEMIKRHQKFSLFERRLEKNQVL 371
V + ++ + Q + +FE + + + V+
Sbjct: 479 YVRVLQRFDERSKLFENLQNYRIFEFKAKPHTVV 580
>TC8280
Length = 502
Score = 26.6 bits (57), Expect = 7.1
Identities = 15/40 (37%), Positives = 24/40 (59%), Gaps = 5/40 (12%)
Frame = -3
Query: 67 KFHDLNRRFKDDV-PPPQQNGDVS----AVTAEDSDHVPW 101
KF + NR+F +D+ PPP ++ D S +++ SD PW
Sbjct: 122 KFLEKNRKFSNDIKPPPLKSVDGSLACQSISFHWSDIKPW 3
>AV769448
Length = 447
Score = 26.6 bits (57), Expect = 7.1
Identities = 12/32 (37%), Positives = 22/32 (68%)
Frame = -1
Query: 330 AKMENEDVAVVKSEPLFGVLEMIKRHQKFSLF 361
AK++N+ +++ K+ P L+ + +HQ FSLF
Sbjct: 357 AKLQNK-LSMKKAHPQDAQLQQVNQHQMFSLF 265
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.306 0.126 0.345
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,353,578
Number of Sequences: 28460
Number of extensions: 62514
Number of successful extensions: 252
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 248
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 251
length of query: 371
length of database: 4,897,600
effective HSP length: 92
effective length of query: 279
effective length of database: 2,279,280
effective search space: 635919120
effective search space used: 635919120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (22.0 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146756.1