BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146752.14 - phase: 2 /pseudo
(1086 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18710 81 8e-16
BP041113 79 5e-15
AV428268 77 1e-14
TC13572 70 2e-12
AV412895 67 2e-11
BP056597 67 2e-11
AV414139 63 3e-10
BP078534 51 1e-06
AU089037 50 2e-06
TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane pro... 46 3e-05
AV418280 46 4e-05
BP066992 35 5e-05
AV411435 44 2e-04
BP028574 43 3e-04
AV427022 43 3e-04
AU089120 43 3e-04
TC20217 41 0.001
AV780221 39 0.003
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 38 0.010
AV777945 37 0.017
>TC18710
Length = 843
Score = 81.3 bits (199), Expect = 8e-16
Identities = 44/119 (36%), Positives = 65/119 (53%), Gaps = 3/119 (2%)
Frame = +2
Query: 541 QSAFIKGRQILDGILIANELVDDAKRNN---KELLMFKVDFEKAYDSVDWRYLDEVMAKM 597
QS FI+GRQI D +LI E R + + K+D KAYD V+W++L+ +
Sbjct: 458 QSGFIQGRQIQDNLLIVQEAFHAINRPGALGRNHSIIKLDMNKAYDRVEWKFLESSLLAF 637
Query: 598 NFPRLWRSWIMECVTTATTSVLVNGCPTDEFHFERGLRQGDPLSPFLFLLAAEGLNVMM 656
F W IM V+ + + +NG + +RGLRQGDP SP+LFL E L++++
Sbjct: 638 GFSTNWVKMIMILVSGVSYNYKINGVVGPKLLPQRGLRQGDPFSPYLFLFTMEVLSLLI 814
>BP041113
Length = 503
Score = 78.6 bits (192), Expect = 5e-15
Identities = 57/171 (33%), Positives = 76/171 (44%), Gaps = 3/171 (1%)
Frame = +1
Query: 848 FWEDPWLLNVPLAVSFSRLFELAENKGVSVREMFLLGWGADGGAWR--WRRRLFAWEEEL 905
FW + W+ L + RLF L++ K + E GW W+ WRR L E
Sbjct: 1 FWTEDWIGMGTLRDRYYRLFNLSKEKRCCILECG--GWNHGVWTWKLSWRRALVGRELGW 174
Query: 906 VEECVARLSNFVLQADLSDRWVWRLHSSQLYTVQSAYSYLTAVDSNITAEFDRFLWLKAV 965
+E + LS L + D+W+W + YTV SAYS+L A T +W
Sbjct: 175 LELMMKDLSGVCLSEGVEDKWLWLPGGT--YTVNSAYSFLQAPTLADTDPIFATIWRTVA 348
Query: 966 PLKVNIFVWRLFLNRLATKDNLRKRNVLEATNVSSGALC-GKEEERDHLFF 1015
P V F WR L RL T DNL KR V+ ++ C G+ E HL F
Sbjct: 349 P-SVKAFAWRCLLGRLPTYDNLIKRQVVVDPAMTVCKFCQGEVESVTHLLF 498
>AV428268
Length = 429
Score = 77.0 bits (188), Expect = 1e-14
Identities = 49/144 (34%), Positives = 72/144 (49%), Gaps = 1/144 (0%)
Frame = +3
Query: 936 YTVQSAYSYLTAVDSNITAEFDRFLWLKAVPLKVNIFVWRLFLNRLATKDNLRKRNVLEA 995
Y+V+SAY +L + + LW VP W++ +NR+ TK NL +R V+
Sbjct: 6 YSVKSAYDFLHGCANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVV-I 182
Query: 996 TNVSSGALCGKEEER-DHLFFQCDYYGRLWLLISNWLGFVTVFHDNLYTHANQFCALGGF 1054
+NV LC +EE DHL F C R+W IS G +VF ++ + H Q G
Sbjct: 183 SNVCP--LCSLDEESTDHLLFSCPIV*RIWSKISE*FGVFSVFPNDSHGHFLQHLGSCG- 353
Query: 1055 SKNYMKAFTIIWISVLFTIWKDRN 1078
S N+ K +WI+ + IW+ RN
Sbjct: 354 SLNFRKRGWFVWIAAVVCIWQGRN 425
>TC13572
Length = 534
Score = 70.1 bits (170), Expect = 2e-12
Identities = 48/160 (30%), Positives = 72/160 (45%), Gaps = 2/160 (1%)
Frame = -3
Query: 788 WNVVLCAKYGEVGGRVQFSEGVGPIWWRQLNQIRCGVGLAENAWLVDNIVRKVGDGSTTL 847
W V+ AKYG+ GG S WWR + G EN W + + + + G+ T
Sbjct: 478 WCRVVLAKYGD-GGCTNISS-----WWRDVQT----AGGGENGWFEEGVHKVLRSGNHTR 329
Query: 848 FWEDPWLLNVPLAVSFSRLFELAENKGVSVREMFLLGWGADGGAW--RWRRRLFAWEEEL 905
FW + W L + RL+ L++ K S+ E GW W RWRR L E +
Sbjct: 328 FWLENWTGVGILREKYYRLYNLSKLKWASIDECG--GWSQGVWNWDFRWRRPLAGRELDW 155
Query: 906 VEECVARLSNFVLQADLSDRWVWRLHSSQLYTVQSAYSYL 945
++ + L A + D+WVW+ Y+V SA+ +L
Sbjct: 154 LQALSLDIVRVPLLAGVPDKWVWKPSEDGSYSVNSAFIFL 35
>AV412895
Length = 405
Score = 67.0 bits (162), Expect = 2e-11
Identities = 39/109 (35%), Positives = 52/109 (46%), Gaps = 1/109 (0%)
Frame = +1
Query: 960 LWLKAVPLKVNIFVWRLFLNRLATKDNLRKRNVLEATNVSSGALCG-KEEERDHLFFQCD 1018
+W+ PL + FVWR+ L R+ T+DNL KR V+ + LCG EE HL F C
Sbjct: 82 IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIHNALDAICPLCGLAEESGSHLLFSCA 261
Query: 1019 YYGRLWLLISNWLGFVTVFHDNLYTHANQFCALGGFSKNYMKAFTIIWI 1067
+W WLG T + H QF ++G SK T IW+
Sbjct: 262 ESMLIWYECFAWLGVSTAQVSDPKVHLLQFSSIGR-SKAQKLGETTIWM 405
>BP056597
Length = 414
Score = 66.6 bits (161), Expect = 2e-11
Identities = 44/138 (31%), Positives = 65/138 (46%), Gaps = 7/138 (5%)
Frame = +3
Query: 862 SFSRLFELAENKGVSVREMFLLGWGADGGAWRWRRRLFAWEEELVEECVARL-------S 914
+F LF ++ + + EM G + W W F+W+ + E V +L S
Sbjct: 6 TFDSLFLISNQQNCKIVEM---GHWLEN-KWVWD---FSWKNPISGEDVLKLGVMKQIVS 164
Query: 915 NFVLQADLSDRWVWRLHSSQLYTVQSAYSYLTAVDSNITAEFDRFLWLKAVPLKVNIFVW 974
+F L D WVW L Y+V+SAY L+ +D+ I LW P W
Sbjct: 165 SFSLINGKKDTWVWILEGDGQYSVKSAYDLLSGLDTTIGISVFSKLWKACAPSNAVALGW 344
Query: 975 RLFLNRLATKDNLRKRNV 992
R+FL+R+ TKDNL +R+V
Sbjct: 345 RVFLDRIQTKDNLSRRHV 398
>AV414139
Length = 309
Score = 62.8 bits (151), Expect = 3e-10
Identities = 33/93 (35%), Positives = 45/93 (47%), Gaps = 4/93 (4%)
Frame = -2
Query: 890 GAW----RWRRRLFAWEEELVEECVARLSNFVLQADLSDRWVWRLHSSQLYTVQSAYSYL 945
G W RWRR L E+ + + L+ LQ DRW W L + +YTV+SAY L
Sbjct: 281 GVWEWEFRWRRELSVREQRQEADLIQALAGVCLQQGTEDRWKWTLDTEDIYTVRSAY*LL 102
Query: 946 TAVDSNITAEFDRFLWLKAVPLKVNIFVWRLFL 978
++ +E +W+ P N FVWRL L
Sbjct: 101 LDPIADSDSEVYEKVWVGIAPSNANAFVWRLLL 3
>BP078534
Length = 410
Score = 50.8 bits (120), Expect = 1e-06
Identities = 26/61 (42%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
Frame = -3
Query: 849 WEDPWLLNVPLAVSFSRLFELAENKGVSVREMFLLGWGADGGAWRW----RRRLFAWEEE 904
W DPW+ L F RLF L ENKG V ++ GG+W W RR F WEEE
Sbjct: 216 WLDPWIRERALCDMFPRLFTLCENKGALVADV----GSYHGGSWHWNLSFRREFFQWEEE 49
Query: 905 L 905
+
Sbjct: 48 I 46
>AU089037
Length = 245
Score = 49.7 bits (117), Expect = 2e-06
Identities = 23/59 (38%), Positives = 33/59 (54%), Gaps = 1/59 (1%)
Frame = -1
Query: 960 LWLKAVPLKVNIFVWRLFLNRLATKDNLRKRNVLEATNVSSGALCGK-EEERDHLFFQC 1017
LW P F WRL L+R+ T+ NLR+R V++ + + +C + EE HLFF C
Sbjct: 191 LWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLFFSC 15
>TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane protein-like,
partial (10%)
Length = 879
Score = 46.2 bits (108), Expect = 3e-05
Identities = 25/76 (32%), Positives = 33/76 (42%), Gaps = 4/76 (5%)
Frame = -1
Query: 890 GAWRW----RRRLFAWEEELVEECVARLSNFVLQADLSDRWVWRLHSSQLYTVQSAYSYL 945
G W W RR E LV + L + L D +D W W + YTVQ+AY +L
Sbjct: 774 GVWTWSFIRRRNPSTRENLLVADLCNLLESVPLSEDTNDSWSWTANPEGQYTVQTAYKFL 595
Query: 946 TAVDSNITAEFDRFLW 961
A + T F+W
Sbjct: 594 RASSNEFTDPIFHFVW 547
>AV418280
Length = 398
Score = 45.8 bits (107), Expect = 4e-05
Identities = 24/78 (30%), Positives = 38/78 (47%)
Frame = -2
Query: 1007 EEERDHLFFQCDYYGRLWLLISNWLGFVTVFHDNLYTHANQFCALGGFSKNYMKAFTIIW 1066
EE HLFF C + +W + WLG + + QF ++ +KN IW
Sbjct: 367 EECSGHLFFTCVFSMGVWQALHRWLGISVALPASTLANFAQF-SITARNKNQRLGELAIW 191
Query: 1067 ISVLFTIWKDRNRRIFQN 1084
I+ ++++W RN IF+N
Sbjct: 190 IATVWSLWIQRNSIIFRN 137
>BP066992
Length = 525
Score = 35.0 bits (79), Expect(2) = 5e-05
Identities = 16/36 (44%), Positives = 22/36 (60%)
Frame = +2
Query: 960 LWLKAVPLKVNIFVWRLFLNRLATKDNLRKRNVLEA 995
LW P+ FVWR+ LNR+ TK LRK N++ +
Sbjct: 5 LWKVVAPIS-QCFVWRVMLNRIQTKTYLRK*NIINS 109
Score = 29.6 bits (65), Expect(2) = 5e-05
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +1
Query: 1006 KEEERDHLFFQCDYYGRLWLLISNWLGFVTVFHDNLYTHANQ 1047
+EE HL F C + + WLGF TV +L +A +
Sbjct: 142 EEETTSHLLFTCSLSSIVCNICYKWLGFHTVLSFDLINYAEE 267
>AV411435
Length = 426
Score = 43.5 bits (101), Expect = 2e-04
Identities = 32/112 (28%), Positives = 49/112 (43%), Gaps = 11/112 (9%)
Frame = +3
Query: 924 DRWVWRLHSSQLYTVQSAYSYLTAVDSNIT------AEFDRFLWLKA----VPLKVNIFV 973
DR W S Y+V+S Y + A N++ + FLW VP KV F+
Sbjct: 39 DRLFWPFIPSGEYSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHTIWGAQVPKKVRSFL 218
Query: 974 WRLFLNRLATKDNLRKRNVLEATNVSSGALCGK-EEERDHLFFQCDYYGRLW 1024
WR N + K NL++RN+ +C K E+ +H C++ +W
Sbjct: 219 WRAANNAIPVKRNLKRRNM---GRDDFCPICNKGPEDINHALLACEWTRAVW 365
>BP028574
Length = 454
Score = 42.7 bits (99), Expect = 3e-04
Identities = 24/65 (36%), Positives = 29/65 (43%)
Frame = +3
Query: 917 VLQADLSDRWVWRLHSSQLYTVQSAYSYLTAVDSNITAEFDRFLWLKAVPLKVNIFVWRL 976
VL + D WVW S +TV+SAY L I F R LW P WR+
Sbjct: 240 VLSINHRDSWVWIKDGSGSFTVKSAYEELHGEIQEIEDNFFRKLWQSKAPSNSIALAWRV 419
Query: 977 FLNRL 981
LNR+
Sbjct: 420 GLNRV 434
>AV427022
Length = 429
Score = 42.7 bits (99), Expect = 3e-04
Identities = 27/79 (34%), Positives = 37/79 (46%)
Frame = +1
Query: 1004 CGKEEERDHLFFQCDYYGRLWLLISNWLGFVTVFHDNLYTHANQFCALGGFSKNYMKAFT 1063
C EE HL F C + WLG T + H QF ++G +SK +
Sbjct: 52 CLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLLQFSSIG-WSKAQKLGES 228
Query: 1064 IIWISVLFTIWKDRNRRIF 1082
IW+SVL++IW RN +F
Sbjct: 229 AIWMSVLWSIWCLRNMVVF 285
>AU089120
Length = 586
Score = 42.7 bits (99), Expect = 3e-04
Identities = 26/99 (26%), Positives = 42/99 (42%)
Frame = +2
Query: 987 LRKRNVLEATNVSSGALCGKEEERDHLFFQCDYYGRLWLLISNWLGFVTVFHDNLYTHAN 1046
L++R +++ S EE HLFF+C LW + WL F V + H
Sbjct: 8 LKRRRIIQTL---SSFCLHSEESIPHLFFRCFQSWNLWSSVHRWLVFTVVTPEEGKMHFI 178
Query: 1047 QFCALGGFSKNYMKAFTIIWISVLFTIWKDRNRRIFQNQ 1085
F ++ IW+S ++ IW RN+ +F +
Sbjct: 179 MF*DYYS-GRDIKNGLGFIWLSTIWFIWHLRNKVVFNGR 292
>TC20217
Length = 561
Score = 40.8 bits (94), Expect = 0.001
Identities = 23/72 (31%), Positives = 34/72 (46%)
Frame = +1
Query: 1013 LFFQCDYYGRLWLLISNWLGFVTVFHDNLYTHANQFCALGGFSKNYMKAFTIIWISVLFT 1072
L F C +W W+G T N H F GG +K + + IW++V++T
Sbjct: 304 LLFSCPVSLDIWRHCYRWMGVCTTLPRNPRQHLL*F-QFGG-NKKHQRGADAIWLAVIWT 477
Query: 1073 IWKDRNRRIFQN 1084
+W RN IF+N
Sbjct: 478 LWLIRNEIIFRN 513
>AV780221
Length = 461
Score = 39.3 bits (90), Expect = 0.003
Identities = 21/75 (28%), Positives = 34/75 (45%)
Frame = +3
Query: 780 VLEDRESLWNVVLCAKYGEVGGRVQFSEGVGPIWWRQLNQIRCGVGLAENAWLVDNIVRK 839
+L++R+ LW VL KY + S WW L+ G W+ + R+
Sbjct: 255 MLKERDGLWYKVLLIKYKNAIPQSASS------WWNDLHSTCFEDG--GGGWMQRGLCRR 410
Query: 840 VGDGSTTLFWEDPWL 854
+G+G+ FW + WL
Sbjct: 411 IGEGTEVKFWNENWL 455
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 37.7 bits (86), Expect = 0.010
Identities = 22/78 (28%), Positives = 43/78 (54%)
Frame = -3
Query: 678 VSHLQFADDTLLIGVKSWANVRAMKTVLLLFEDVSGLKVNFHKSMFFGVNINESWLHEAA 737
+SHL FAD+ ++ ++ + VL +F+ +SGL N KS F + + E+ ++
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSEVF-ILVKETTKNQIT 197
Query: 738 VVMHCRHGRIPFIYLGLP 755
++ + G++P LG+P
Sbjct: 196 NMLGYKEGKLPVR*LGVP 143
>AV777945
Length = 279
Score = 37.0 bits (84), Expect = 0.017
Identities = 15/35 (42%), Positives = 19/35 (53%)
Frame = +1
Query: 959 FLWLKAVPLKVNIFVWRLFLNRLATKDNLRKRNVL 993
FLW P W + + +L TKD LRKRNV+
Sbjct: 157 FLWASKAPSNSLALAWNVLIKKLQTKDELRKRNVI 261
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.347 0.155 0.542
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,488,652
Number of Sequences: 28460
Number of extensions: 358806
Number of successful extensions: 3971
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 3836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3955
length of query: 1086
length of database: 4,897,600
effective HSP length: 100
effective length of query: 986
effective length of database: 2,051,600
effective search space: 2022877600
effective search space used: 2022877600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146752.14