BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146751.9 - phase: 0
(186 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CN825120 75 9e-15
TC14831 homologue to UP|P93499 (P93499) DnaJ-like protein (Fragm... 69 4e-13
AV767621 62 5e-11
TC16999 similar to UP|Q9T024 (Q9T024) DNAJ-like protein (AT4g391... 59 4e-10
AV411199 58 9e-10
TC15224 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein ... 57 1e-09
BP039305 57 1e-09
TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial ... 57 2e-09
TC12876 56 3e-09
AV775648 55 7e-09
TC8761 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein P... 55 1e-08
TC13524 similar to UP|O48678 (O48678) F3I6.4 protein, partial (19%) 51 1e-07
TC16983 similar to UP|Q9ZSY2 (Q9ZSY2) ALTERED response to gravit... 48 9e-07
BP059356 35 1e-06
BP035098 36 0.004
BP037331 34 0.013
TC8367 similar to GB|AAM65667.1|21593700|AY088122 J8-like protei... 34 0.018
BP050331 34 0.018
TC19451 similar to GB|AAM65667.1|21593700|AY088122 J8-like prote... 33 0.030
TC15462 33 0.039
>CN825120
Length = 667
Score = 74.7 bits (182), Expect = 9e-15
Identities = 37/80 (46%), Positives = 49/80 (61%)
Frame = +3
Query: 47 ATFNDGMAAEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQV 106
+T DG A S Y++LGI + S +IK AY++LAR HPDV+ DR T F+++
Sbjct: 168 STHGDGDMASCTSLYEILGIAAAASDQEIKAAYRRLARVRHPDVAAVDRKDSSTNEFMKI 347
Query: 107 QEAYETLSDPSRRIMYDRDM 126
AY TLSDP +R YDR M
Sbjct: 348 HAAYSTLSDPEKRASYDRSM 407
>TC14831 homologue to UP|P93499 (P93499) DnaJ-like protein (Fragment),
partial (63%)
Length = 851
Score = 69.3 bits (168), Expect = 4e-13
Identities = 32/68 (47%), Positives = 43/68 (63%)
Frame = +3
Query: 59 SFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSR 118
S Y++LGIP S +IK AY++LAR HPDV+ DR F+++ AY TLSDP +
Sbjct: 243 SLYEILGIPAGASSQEIKAAYRRLARVCHPDVAAIDRKNSSADDFMKIHAAYSTLSDPEK 422
Query: 119 RIMYDRDM 126
R YDR +
Sbjct: 423 RATYDRSL 446
>AV767621
Length = 560
Score = 62.4 bits (150), Expect = 5e-11
Identities = 28/65 (43%), Positives = 45/65 (69%)
Frame = +1
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y +L + S D+K+AY++LA K+HPD +P ++ KE +F Q+ EAY+ LSDP +R
Sbjct: 64 YYKILQVDRGASDDDLKKAYRKLAMKWHPDKNPNNK-KEAEAKFKQISEAYDVLSDPQKR 240
Query: 120 IMYDR 124
+YD+
Sbjct: 241 AVYDQ 255
>TC16999 similar to UP|Q9T024 (Q9T024) DNAJ-like protein
(AT4g39150/T22F8_50), partial (35%)
Length = 648
Score = 59.3 bits (142), Expect = 4e-10
Identities = 31/75 (41%), Positives = 45/75 (59%)
Frame = +3
Query: 56 EERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSD 115
+E ++YD+LG+ S DIK+AY AR HPD +P D + + F + EAY+ LSD
Sbjct: 117 KESAYYDVLGVNVDASAADIKKAYYIKARIVHPDKNPGD--PKAAENFQMLGEAYQVLSD 290
Query: 116 PSRRIMYDRDMAIGI 130
P +R YD++ GI
Sbjct: 291 PEKREAYDKNGKAGI 335
>AV411199
Length = 423
Score = 58.2 bits (139), Expect = 9e-10
Identities = 26/65 (40%), Positives = 44/65 (67%)
Frame = +2
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y++L + + ++K+AY++LA K+HPD +P ++ TK F + E+YE LSDP +R
Sbjct: 71 YYEILEVDHHATDEELKKAYRRLAMKWHPDKNPDNKNDAETK-FKLISESYEVLSDPQKR 247
Query: 120 IMYDR 124
+YDR
Sbjct: 248 AIYDR 262
>TC15224 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein PM37,
partial (71%)
Length = 1031
Score = 57.4 bits (137), Expect = 1e-09
Identities = 28/71 (39%), Positives = 46/71 (64%), Gaps = 1/71 (1%)
Frame = +3
Query: 55 AEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDV-SPPDRVKEYTKRFIQVQEAYETL 113
++ +Y++LG+P++ S D+K+AYK+ A K HPD P++ KE + +AYE L
Sbjct: 162 SDSTRYYEILGVPKNASQDDLKKAYKKAAIKNHPDKGGDPEKFKE-------LAQAYEVL 320
Query: 114 SDPSRRIMYDR 124
SDP +R +YD+
Sbjct: 321 SDPEKREIYDQ 353
>BP039305
Length = 555
Score = 57.4 bits (137), Expect = 1e-09
Identities = 27/65 (41%), Positives = 42/65 (64%)
Frame = +3
Query: 55 AEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLS 114
A +Y+ LG+P+S ++ +IK AY++LAR+YHPDV + T +F ++ AYE LS
Sbjct: 369 AASADYYNTLGVPKSATVKEIKAAYRRLARQYHPDVY---KEPGATDKFKEISNAYEVLS 539
Query: 115 DPSRR 119
D +R
Sbjct: 540 DDKKR 554
>TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial (90%)
Length = 1737
Score = 57.0 bits (136), Expect = 2e-09
Identities = 28/71 (39%), Positives = 46/71 (64%), Gaps = 1/71 (1%)
Frame = +1
Query: 55 AEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDV-SPPDRVKEYTKRFIQVQEAYETL 113
++ FY++LG+P+S S +IK+AY++ A K HPD P++ KE + +AYE L
Sbjct: 172 SDNTKFYEVLGVPKSASEDEIKKAYRKAAMKNHPDKGGDPEKFKE-------LGQAYEVL 330
Query: 114 SDPSRRIMYDR 124
SDP ++ +YD+
Sbjct: 331 SDPEKKELYDQ 363
>TC12876
Length = 820
Score = 56.2 bits (134), Expect = 3e-09
Identities = 28/72 (38%), Positives = 44/72 (60%), Gaps = 4/72 (5%)
Frame = -1
Query: 56 EERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDR----VKEYTKRFIQVQEAYE 111
+ FY +LG+ + + +++ AYK+LA K+HPD V+E K+F +QEAY
Sbjct: 244 KSNDFYAVLGLNKECTESELRNAYKKLALKWHPDRCSASGNLKFVEEAKKKFQSIQEAYS 65
Query: 112 TLSDPSRRIMYD 123
LSD ++R+MYD
Sbjct: 64 VLSDANKRLMYD 29
>AV775648
Length = 371
Score = 55.1 bits (131), Expect = 7e-09
Identities = 28/83 (33%), Positives = 47/83 (55%)
Frame = +1
Query: 56 EERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSD 115
+E YDL+G+ ++ + +IK+AY +L+ K+HPD +P K K ++V AYE L D
Sbjct: 130 DEDDCYDLVGVSQNANSSEIKKAYYKLSLKHHPDTNPDPESK---KLLVKVANAYEILKD 300
Query: 116 PSRRIMYDRDMAIGIHLAFNACK 138
+ R YD +A + +N +
Sbjct: 301 EATREQYDYAIAHPEEVFYNTAR 369
>TC8761 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein PM37,
partial (74%)
Length = 1050
Score = 54.7 bits (130), Expect = 1e-08
Identities = 35/95 (36%), Positives = 54/95 (56%), Gaps = 5/95 (5%)
Frame = +3
Query: 34 TRISFGASFPKLKATFNDGMAAEERS----FYDLLGIPESGSLMDIKRAYKQLARKYHPD 89
TR+S F + A G A ++S +YD+LG+ ++ S D+K+AYK+ A K HPD
Sbjct: 39 TRVS---EFRSIGAAKMFGRAPPKKSDSTRYYDILGVSKTASQDDLKKAYKKAAIKNHPD 209
Query: 90 V-SPPDRVKEYTKRFIQVQEAYETLSDPSRRIMYD 123
P++ KE + +AYE L+DP +R +YD
Sbjct: 210 KGGDPEKFKE-------LAQAYEVLNDPEKREIYD 293
>TC13524 similar to UP|O48678 (O48678) F3I6.4 protein, partial (19%)
Length = 485
Score = 50.8 bits (120), Expect = 1e-07
Identities = 26/66 (39%), Positives = 39/66 (58%)
Frame = +1
Query: 58 RSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPS 117
R Y++LG+ S + +IK AY+++A KYHPD + D + F +V +Y LSDP
Sbjct: 289 RDPYEVLGVSRSSTDQEIKTAYRKMALKYHPDKNAND--PKAADMFKEVTFSYNILSDPD 462
Query: 118 RRIMYD 123
+R YD
Sbjct: 463 KRRQYD 480
>TC16983 similar to UP|Q9ZSY2 (Q9ZSY2) ALTERED response to gravity (ARG1
protein), partial (42%)
Length = 716
Score = 48.1 bits (113), Expect = 9e-07
Identities = 25/66 (37%), Positives = 39/66 (58%)
Frame = +2
Query: 58 RSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPS 117
R Y++L + + +IK AY++LA KYHPD + + E ++ F +V +Y LSDP
Sbjct: 236 RDPYEVLSVSRDSTDQEIKTAYRKLALKYHPDKNVNN--PEASELFKEVAYSYSILSDPE 409
Query: 118 RRIMYD 123
+R YD
Sbjct: 410 KRRQYD 427
>BP059356
Length = 436
Score = 35.0 bits (79), Expect(2) = 1e-06
Identities = 14/32 (43%), Positives = 21/32 (64%)
Frame = +1
Query: 93 PDRVKEYTKRFIQVQEAYETLSDPSRRIMYDR 124
P+ KE F ++ EAY+ LSDP +R +YD+
Sbjct: 193 PNNKKEAEANFKKISEAYDVLSDPQKRAVYDQ 288
Score = 32.0 bits (71), Expect(2) = 1e-06
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +3
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSP 92
+Y +L + S D+K+AY++LA K+HP P
Sbjct: 96 YYKVLQVDRSAKDDDLKKAYRKLAMKWHPG*EP 194
>BP035098
Length = 458
Score = 36.2 bits (82), Expect = 0.004
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = -1
Query: 103 FIQVQEAYETLSDPSRRIMYDRDMA 127
FIQ++ AYETLSDPS R YD ++
Sbjct: 440 FIQIRNAYETLSDPSARATYDMSLS 366
>BP037331
Length = 405
Score = 34.3 bits (77), Expect = 0.013
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = +3
Query: 56 EERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDV 90
EE+S Y+ L + IK AY++LA+ YHPDV
Sbjct: 279 EEKSPYETLELERDADEEQIKIAYRRLAKFYHPDV 383
>TC8367 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;}, partial (64%)
Length = 962
Score = 33.9 bits (76), Expect = 0.018
Identities = 18/53 (33%), Positives = 29/53 (53%)
Frame = +2
Query: 61 YDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETL 113
Y L I S ++++A++QLA +YHPDV R +F + EAY+ +
Sbjct: 431 YKTLKIQPGASETEVRKAFRQLALQYHPDVC---RGSNCGVQFHLINEAYDVV 580
>BP050331
Length = 519
Score = 33.9 bits (76), Expect = 0.018
Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +3
Query: 60 FYDLLGIP--ESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPS 117
FY +L + +S L IKR Y++LA HPD + F V A+ LSDP+
Sbjct: 270 FYAILQVDHRDSQDLNLIKRQYRRLALLLHPD---KNLFSFSHHTFDLVSHAWALLSDPA 440
Query: 118 RRIMYDRDMAIGIHLAFNAC 137
++ +YD + + AC
Sbjct: 441 QKEIYDAGLGCAPGSFWTAC 500
>TC19451 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;}, partial (35%)
Length = 480
Score = 33.1 bits (74), Expect = 0.030
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +2
Query: 61 YDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETL 113
Y L + S ++K+A++ LA +YHPDV + +F + EAY+ +
Sbjct: 233 YKTLRVQPGASQTEVKKAFRHLALQYHPDVCQGSKC---GVQFHHINEAYDVV 382
>TC15462
Length = 515
Score = 32.7 bits (73), Expect = 0.039
Identities = 18/53 (33%), Positives = 28/53 (51%), Gaps = 2/53 (3%)
Frame = +2
Query: 63 LLGIPESGSLM--DIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETL 113
+LG+P +G L D+K A++ A K+HPD ++F AY+TL
Sbjct: 77 ILGLPSTGPLKIEDVKNAFRLSALKWHPDKHQGSSQAMAEEKFKLCVNAYKTL 235
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.134 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,132,433
Number of Sequences: 28460
Number of extensions: 36906
Number of successful extensions: 266
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 254
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 254
length of query: 186
length of database: 4,897,600
effective HSP length: 85
effective length of query: 101
effective length of database: 2,478,500
effective search space: 250328500
effective search space used: 250328500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146751.9