BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146751.5 + phase: 0 /pseudo
(455 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV406949 139 1e-33
TC16764 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp prot... 130 3e-31
CB829689 127 3e-30
TC15538 similar to UP|CLPA_PEA (P35100) ATP-dependent Clp protea... 100 4e-22
TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , par... 91 3e-19
AV766174 76 1e-14
TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp prot... 72 1e-13
TC17248 similar to UP|CLPA_PEA (P35100) ATP-dependent Clp protea... 55 2e-08
TC15017 51 3e-07
TC11285 37 0.005
TC19529 similar to UP|CLPA_PEA (P35100) ATP-dependent Clp protea... 37 0.007
BP042523 31 0.37
AV422700 30 1.1
AV778312 29 1.4
TC9990 similar to UP|Q9FJW0 (Q9FJW0) RuvB DNA helicase-like prot... 28 3.1
AV417257 28 3.1
TC16249 similar to UP|O63067 (O63067) Aspartokinase-homoserine d... 28 4.1
TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 27 5.3
TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase... 27 7.0
BP048612 27 9.1
>AV406949
Length = 443
Score = 139 bits (350), Expect = 1e-33
Identities = 82/183 (44%), Positives = 108/183 (58%)
Frame = +1
Query: 87 AKNVLDFSLKHAKSLGYDNVDTMHLLLGLLQGSNGTVTQLIQNQGADVNKIREQVIRQIE 146
AK V + SL+ A+ LG++ + + HLLLGLL+ G +++Q+ GAD N IR QVIR +
Sbjct: 7 AKRVPELSLEEARQLGHNYIGSEHLLLGLLREGEGVAARVLQDLGADPNNIRAQVIRMVG 186
Query: 147 ENVHQVNLPAEGSKNQMGGQVEENVTAESSKSNQIKFPVLTQNNNLGARKDDNANKQKSA 206
E S N+ G + + ++NK +
Sbjct: 187 E-----------SNNETAG--------------------------VAVGRGGSSNKMPT- 252
Query: 207 LEIFGTNLTKLAQEGKLHPFVGREEQVERVIQIICRRMKNNPCLVGEPGVGKTSIIQGLA 266
LE +GTNLTKLA EGKL P VGR++Q+ERVIQI+ RR KNNPCLVGEPGVGKT+I +GLA
Sbjct: 253 LEEYGTNLTKLANEGKLDPVVGRQQQIERVIQILGRRTKNNPCLVGEPGVGKTAIAEGLA 432
Query: 267 QRI 269
QRI
Sbjct: 433 QRI 441
Score = 59.3 bits (142), Expect = 1e-09
Identities = 28/60 (46%), Positives = 43/60 (71%)
Frame = +1
Query: 9 AKKVIMAAQKEARFVGHKYISTEHILLGILGDSNGLAAKILKSYGIDFNVSREQVMKLVG 68
AK+V + +EAR +GH YI +EH+LLG+L + G+AA++L+ G D N R QV+++VG
Sbjct: 7 AKRVPELSLEEARQLGHNYIGSEHLLLGLLREGEGVAARVLQDLGADPNNIRAQVIRMVG 186
>TC16764 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (24%)
Length = 653
Score = 130 bits (328), Expect = 3e-31
Identities = 64/91 (70%), Positives = 78/91 (85%)
Frame = +3
Query: 362 EHRMHMENDTTLKRIFQPVKVVEPSVEETIEILKGLRGTYESHYKLHYTDEALVAAANLS 421
E+R H+E D L+R FQPVKV EP+V+ETI+ILKGLR YE H+KL YTD+ALVAAA+LS
Sbjct: 3 EYRKHIEKDPALERRFQPVKVPEPTVDETIQILKGLRERYEIHHKLRYTDDALVAAAHLS 182
Query: 422 QQYVSERFLPDKAIDLIDEAGSHVQLCHAKV 452
QY+S+RFLPDKAIDLIDEAGS V+L HA++
Sbjct: 183 YQYISDRFLPDKAIDLIDEAGSRVRLRHAQL 275
>CB829689
Length = 558
Score = 127 bits (320), Expect = 3e-30
Identities = 62/142 (43%), Positives = 98/142 (68%), Gaps = 2/142 (1%)
Frame = +1
Query: 197 DDNANKQKSALEIFGTNLTKLAQEGKLHPFVGREEQVERVIQIICRRMKNNPCLVGEPGV 256
D + + ALE +G +LT +A+ GKL P +GR++++ R IQI+ RR KNNP L+GEPGV
Sbjct: 115 DQDPEGKYEALEKYGKDLTAMAKAGKLDPVIGRDDEIRRCIQILSRRTKNNPVLIGEPGV 294
Query: 257 GKTSIIQGLAQRILSGSVPEKLKGKKVVALDVADFLYVISNQGSSEERIRCLIKEI-ELC 315
GKT+I +GLAQRI+ G VP+ L +++++LD+ + +G E+R++ ++KE+ E
Sbjct: 295 GKTAISEGLAQRIVQGDVPQALMNRRLISLDMGALIAGAKYRGEFEDRLKAVLKEVTESD 474
Query: 316 GNVILFVKEVHHIFDA-ATSGA 336
G +LF+ E+H + A AT+GA
Sbjct: 475 GQTVLFIDEIHTVVGAGATNGA 540
>TC15538 similar to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (21%)
Length = 658
Score = 100 bits (250), Expect = 4e-22
Identities = 53/104 (50%), Positives = 73/104 (69%)
Frame = +1
Query: 1 MLENLTECAKKVIMAAQKEARFVGHKYISTEHILLGILGDSNGLAAKILKSYGIDFNVSR 60
M E TE A KVIM AQ+EAR +GH ++ TE ILLG++G+ G+AAK+LKS GI+ +R
Sbjct: 352 MFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDAR 531
Query: 61 EQVMKLVGRGGGCSGFSCKDIRFTFDAKNVLDFSLKHAKSLGYD 104
+V K++GRG SGF +I FT AK VL+ SL+ A+ LG++
Sbjct: 532 VEVEKIIGRG---SGFVAVEIPFTPRAKRVLELSLEEARQLGHN 654
Score = 35.8 bits (81), Expect = 0.015
Identities = 25/90 (27%), Positives = 45/90 (49%), Gaps = 1/90 (1%)
Frame = +1
Query: 53 GIDFNVSREQVMKLVGRGGGCSGF-SCKDIRFTFDAKNVLDFSLKHAKSLGYDNVDTMHL 111
G+DF+ S+ + RG G RFT A V+ + + A+ LG++ V T +
Sbjct: 274 GLDFH-SKVSIATSSRRGKATRGVPKAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQI 450
Query: 112 LLGLLQGSNGTVTQLIQNQGADVNKIREQV 141
LLGL+ G +++++ G ++ R +V
Sbjct: 451 LLGLIGEGTGIAAKVLKSMGINLKDARVEV 540
>TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , partial (28%)
Length = 1001
Score = 91.3 bits (225), Expect = 3e-19
Identities = 73/266 (27%), Positives = 128/266 (47%), Gaps = 34/266 (12%)
Frame = +3
Query: 82 RFTFDAKNVLDFSLKHAKSLGYDNVDTMHLLLGLLQGSNGTVTQLIQNQ-GADVNKIREQ 140
+FT L + + A S G+ + +HL L+ NG Q I N G + + E+
Sbjct: 213 KFTHKTNEALAGAHELAMSSGHAQMTPLHLASTLISDPNGIFFQAISNSSGEESARAVER 392
Query: 141 VIRQIEENVHQVNLP----------------AEGSKNQMG-----------GQVEENVTA 173
V+ Q + + + P A+ ++ G G +E++
Sbjct: 393 VLNQALKKLPSQSPPPDEIPASTTLIKAIRRAQAAQKSRGDTHLAVDQLILGILEDSQIG 572
Query: 174 ESSK-----SNQIKFPVLTQNNNLGARKDD-NANKQKSALEIFGTNLTKLAQEGKLHPFV 227
+ K + ++K + +G + + + + AL+ +G +L + Q GKL P +
Sbjct: 573 DLLKEAGVAAAKVKSELDKLRGKVGKKVESASGDTTFQALKTYGRDLVE--QAGKLDPVI 746
Query: 228 GREEQVERVIQIICRRMKNNPCLVGEPGVGKTSIIQGLAQRILSGSVPEKLKGKKVVALD 287
GR+E++ RV++I+ RR KNNP L+GEPGVGKT++++GLAQRI+ G VP ++
Sbjct: 747 GRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPSIFLMLGLLHWT 926
Query: 288 VADFLYVISNQGSSEERIRCLIKEIE 313
+L V+S G + R ++KE+E
Sbjct: 927 WVLWLLVLS-IGENLRRGEAVLKEVE 1001
>AV766174
Length = 416
Score = 75.9 bits (185), Expect = 1e-14
Identities = 37/93 (39%), Positives = 62/93 (65%), Gaps = 1/93 (1%)
Frame = -2
Query: 250 LVGEPGVGKTSIIQGLAQRILSGSVPEKLKGKKVVALDVADFLYVISNQGSSEERIRCLI 309
L+GEPGVGKT+I +GLAQRI+ G VP+ L +++++LD+ + +G E+R++ ++
Sbjct: 412 LIGEPGVGKTAISEGLAQRIVQGDVPQALMNRRLISLDMGALIAGAKYRGEFEDRLKAVL 233
Query: 310 KEI-ELCGNVILFVKEVHHIFDAATSGARSFAY 341
KE+ E G +LF+ E+H + A +G SF +
Sbjct: 232 KEVTESDGQTVLFIDEIHTVVGAG-NGI*SFPF 137
>TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (33%)
Length = 908
Score = 72.4 bits (176), Expect = 1e-13
Identities = 35/46 (76%), Positives = 41/46 (89%)
Frame = +1
Query: 407 LHYTDEALVAAANLSQQYVSERFLPDKAIDLIDEAGSHVQLCHAKV 452
L YTDE+LVAAA LS QY+S+RFLPDKAIDLIDEAGS V+L HA++
Sbjct: 1 LRYTDESLVAAAQLSYQYISDRFLPDKAIDLIDEAGSRVRLQHAQL 138
>TC17248 similar to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (11%)
Length = 515
Score = 55.5 bits (132), Expect = 2e-08
Identities = 25/46 (54%), Positives = 34/46 (73%)
Frame = +3
Query: 1 MLENLTECAKKVIMAAQKEARFVGHKYISTEHILLGILGDSNGLAA 46
M E TE A KVIM AQ+EAR +GH ++ TE +LLG++G+ G+AA
Sbjct: 378 MFERFTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 515
Score = 26.9 bits (58), Expect = 7.0
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +3
Query: 82 RFTFDAKNVLDFSLKHAKSLGYDNVDTMHLLLGLLQGSNG 121
RFT A V+ + + A+ +G++ V T +LLGL+ G
Sbjct: 387 RFTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTG 506
>TC15017
Length = 1155
Score = 51.2 bits (121), Expect = 3e-07
Identities = 37/130 (28%), Positives = 64/130 (48%), Gaps = 1/130 (0%)
Frame = +2
Query: 11 KVIMAAQKEARFVGHKYISTEHILLGILGDSNGLAAKILKSYGIDFNVSREQVMKLVGRG 70
K + EAR + + TE +L+GIL + AAK L++ GI R++ ++L+G+
Sbjct: 284 KSFAMGELEARKLKYPTTGTEALLMGILVEGTSKAAKFLRANGITLFKVRDETVELLGK- 460
Query: 71 GGCSGFSCKDIRFTFDAKNVLDFSL-KHAKSLGYDNVDTMHLLLGLLQGSNGTVTQLIQN 129
FS + T A+ LD+++ + KS G ++ HLLLG+ Q++
Sbjct: 461 SDMYFFSPEHPPLTEPARKALDWAIDEKLKSGGEGEINVAHLLLGIWSQKESAGQQILAG 640
Query: 130 QGADVNKIRE 139
G + K +E
Sbjct: 641 LGFNDEKAKE 670
Score = 27.7 bits (60), Expect = 4.1
Identities = 18/65 (27%), Positives = 30/65 (45%), Gaps = 1/65 (1%)
Frame = +2
Query: 5 LTECAKKVI-MAAQKEARFVGHKYISTEHILLGILGDSNGLAAKILKSYGIDFNVSREQV 63
LTE A+K + A ++ + G I+ H+LLGI +IL G + ++E
Sbjct: 497 LTEPARKALDWAIDEKLKSGGEGEINVAHLLLGIWSQKESAGQQILAGLGFNDEKAKELA 676
Query: 64 MKLVG 68
+ G
Sbjct: 677 KSVDG 691
>TC11285
Length = 456
Score = 37.4 bits (85), Expect = 0.005
Identities = 19/61 (31%), Positives = 35/61 (57%)
Frame = +1
Query: 9 AKKVIMAAQKEARFVGHKYISTEHILLGILGDSNGLAAKILKSYGIDFNVSREQVMKLVG 68
A K + EAR + + TE +L+G+L + +AAK L++ G+ R++ +KL+G
Sbjct: 265 AIKSFAMGELEARKLKYPTTGTEALLMGVLIEGTNVAAKFLRANGVTLFKVRDETVKLLG 444
Query: 69 R 69
+
Sbjct: 445 K 447
>TC19529 similar to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (8%)
Length = 560
Score = 37.0 bits (84), Expect = 0.007
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = -1
Query: 1 MLENLTECAKKVIMAAQKEARFVGHKYISTE 31
M E TE A KVIM +Q+EAR +GH ++ TE
Sbjct: 95 MFERFTEKAIKVIMLSQEEARRLGHNFVGTE 3
>BP042523
Length = 479
Score = 31.2 bits (69), Expect = 0.37
Identities = 22/57 (38%), Positives = 31/57 (53%), Gaps = 2/57 (3%)
Frame = +1
Query: 222 KLHPFVGREEQVERVIQIICRRMKNNPCLV--GEPGVGKTSIIQGLAQRILSGSVPE 276
K+ VG ++ V R +Q+I R N P L+ G PG GKT+ I LA +L + E
Sbjct: 103 KVADIVGNQDAVSR-LQVIARD-GNMPNLILSGPPGTGKTTSILALAHELLGPNCKE 267
>AV422700
Length = 472
Score = 29.6 bits (65), Expect = 1.1
Identities = 24/87 (27%), Positives = 35/87 (39%)
Frame = +2
Query: 181 IKFPVLTQNNNLGARKDDNANKQKSALEIFGTNLTKLAQEGKLHPFVGREEQVERVIQII 240
I+ VL + N+N Q A E G N L +L F R R + +
Sbjct: 125 IRVGVLCSVKMESSMSSSNSNNQWRAEEAIGGNTEALEALRELIIFPQRFSHEARKLGL- 301
Query: 241 CRRMKNNPCLVGEPGVGKTSIIQGLAQ 267
+ L G PG GKTS+++ + Q
Sbjct: 302 --KWPRGLLLYGPPGTGKTSLVRAVVQ 376
>AV778312
Length = 610
Score = 29.3 bits (64), Expect = 1.4
Identities = 16/35 (45%), Positives = 21/35 (59%)
Frame = +2
Query: 82 RFTFDAKNVLDFSLKHAKSLGYDNVDTMHLLLGLL 116
RFT A + FS + AK+LG + V HL LGL+
Sbjct: 503 RFTERAIKAIVFSQREAKALGSELVYAQHLWLGLV 607
>TC9990 similar to UP|Q9FJW0 (Q9FJW0) RuvB DNA helicase-like protein
(AT5g67630/K9I9_20), partial (39%)
Length = 642
Score = 28.1 bits (61), Expect = 3.1
Identities = 12/40 (30%), Positives = 25/40 (62%)
Frame = +2
Query: 250 LVGEPGVGKTSIIQGLAQRILSGSVPEKLKGKKVVALDVA 289
L G+PG GKT+I G+A+ + + + G ++ +L+++
Sbjct: 290 LAGQPGTGKTAIAMGMAKSLGLETPFAMIAGSELFSLEMS 409
>AV417257
Length = 345
Score = 28.1 bits (61), Expect = 3.1
Identities = 21/71 (29%), Positives = 33/71 (45%), Gaps = 2/71 (2%)
Frame = +3
Query: 256 VGKTSIIQGLAQRILSGSVPEKLKGK--KVVALDVADFLYVISNQGSSEERIRCLIKEIE 313
+ T +I LA + VPE LKG + A+ + FLY N+ + ++ R + E
Sbjct: 69 IAATQLIGALANSSRASQVPEILKGLGIDIGAVSIFAFLYFRDNKAKNAQQAR--LSREE 242
Query: 314 LCGNVILFVKE 324
N+ L V E
Sbjct: 243 FLSNLKLRVDE 275
>TC16249 similar to UP|O63067 (O63067) Aspartokinase-homoserine
dehydrogenase (HDH) (Aspartate kinase) , partial (13%)
Length = 590
Score = 27.7 bits (60), Expect = 4.1
Identities = 21/83 (25%), Positives = 32/83 (38%), Gaps = 7/83 (8%)
Frame = +3
Query: 195 RKDDNANKQKSALEIFGT-------NLTKLAQEGKLHPFVGREEQVERVIQIICRRMKNN 247
+K ++A L G + +L + K HPF + +I RR +N
Sbjct: 72 KKQEDAESAGEVLRYVGVVDVRNEKGVVELRRYKKDHPFAQLSGS-DNIIAFTTRRYQNQ 248
Query: 248 PCLVGEPGVGKTSIIQGLAQRIL 270
P +V PG G G+ IL
Sbjct: 249 PLIVRGPGAGAQVTAGGIFSDIL 317
>TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (65%)
Length = 901
Score = 27.3 bits (59), Expect = 5.3
Identities = 26/104 (25%), Positives = 41/104 (39%), Gaps = 4/104 (3%)
Frame = +3
Query: 250 LVGEPGVGKTSIIQGLAQRILSGSVPEKLKGKKVVALDVADFLYVISNQGSSEERIRCLI 309
L G PG GKT + + +A + + +VV + +V G +R +
Sbjct: 615 LYGPPGTGKTMLAKAVANHTTAAFI-------RVVGSE-----FVQKYLGEGPRMVRDVF 758
Query: 310 KEIELCGNVILFVKEVHHI----FDAATSGARSFAYILKHALER 349
+ + I+F+ EV I FDA T R IL L +
Sbjct: 759 RLAKENAPAIIFIDEVDAIATARFDAQTGADREVQRILMELLNQ 890
>TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase subunit
RPT2a, partial (69%)
Length = 916
Score = 26.9 bits (58), Expect = 7.0
Identities = 31/126 (24%), Positives = 47/126 (36%), Gaps = 17/126 (13%)
Frame = +1
Query: 250 LVGEPGVGKTSIIQGLAQRILSGSVPEKLKGKKVVALDVADFLYVISNQ------GSSEE 303
L GEPG GKT L K V A FL V+ ++ G +
Sbjct: 499 LYGEPGTGKT------------------LLAKAVANSTSATFLRVVGSELIQKYLGDGPK 624
Query: 304 RIRCLIKEIELCGNVILFVKEVHHI----FDAATSGARSFAYILKHAL-------ERGVI 352
+R L + + I+F+ E+ + +DA + G R + L RG +
Sbjct: 625 LVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDV 804
Query: 353 QCIFAT 358
+ I AT
Sbjct: 805 KVILAT 822
>BP048612
Length = 478
Score = 26.6 bits (57), Expect = 9.1
Identities = 10/28 (35%), Positives = 18/28 (63%)
Frame = -3
Query: 24 GHKYISTEHILLGILGDSNGLAAKILKS 51
G + + EH+ +G++GDS G AA + +
Sbjct: 374 GVRGVVVEHLSVGLIGDSGGAAAVFIST 291
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,335,088
Number of Sequences: 28460
Number of extensions: 74044
Number of successful extensions: 375
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 365
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 374
length of query: 455
length of database: 4,897,600
effective HSP length: 93
effective length of query: 362
effective length of database: 2,250,820
effective search space: 814796840
effective search space used: 814796840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146751.5