BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146751.14 - phase: 1 /pseudo
(387 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB827710 303 3e-83
BP034429 30 0.89
AW428701 29 1.2
TC13083 29 1.5
TC14457 similar to UP|Q8GTZ1 (Q8GTZ1) bZIP transcription factor,... 29 1.5
TC12165 weakly similar to UP|Q9FFY6 (Q9FFY6) Similarity to nasce... 28 2.0
AV777131 28 2.6
TC17313 similar to UP|Q9FH68 (Q9FH68) Genomic DNA, chromosome 5,... 27 4.4
TC15421 similar to GB|AAL36081.1|17380636|AY063731 AT3g49800/T16... 27 5.8
TC14495 UP|O22618 (O22618) Aspartate aminotransferase , complete 27 7.5
CB827458 27 7.5
TC18924 homologue to UP|Q94FN3 (Q94FN3) Phosphatidylinositol tra... 26 9.8
>CB827710
Length = 560
Score = 303 bits (776), Expect = 3e-83
Identities = 162/189 (85%), Positives = 176/189 (92%)
Frame = +1
Query: 192 ERKKTLEESLYSSSPDAHVMLQKLREMQQEEKSISSEIRKREEEHSKLLADLEKQQKVAS 251
ERK+ LEESLYS+SPDA MLQKLRE+QQEE+ ISSEI+ REEEH+KLLADLEKQQKVAS
Sbjct: 1 ERKRNLEESLYSNSPDAQAMLQKLREVQQEEQFISSEIQNREEEHTKLLADLEKQQKVAS 180
Query: 252 RKSYTHRIEEITKNSRKQDADIERILKETREVQLESNSIHERLHRTYAVADEMVFREAKK 311
RKSYTHRI+EITKNSRKQDADIERILKETREVQLESNSI ERLHRTYAVADE+VFREAKK
Sbjct: 181 RKSYTHRIKEITKNSRKQDADIERILKETREVQLESNSIQERLHRTYAVADEIVFREAKK 360
Query: 312 DPTGQQVYKLLVNIHKGFEQISEKILAADRIRREVSEYEMKLAAAPSTSRSLDLSKLQSD 371
DPTG QVY+LLV+IHKGFEQISEKILA DRIRREV+EYEMKLAA ST SLD+SKLQ+D
Sbjct: 361 DPTGLQVYRLLVSIHKGFEQISEKILATDRIRREVAEYEMKLAATAST--SLDVSKLQAD 534
Query: 372 LDAVIIRED 380
LD VIIRE+
Sbjct: 535 LD-VIIREN 558
>BP034429
Length = 567
Score = 29.6 bits (65), Expect = 0.89
Identities = 18/62 (29%), Positives = 30/62 (48%)
Frame = -1
Query: 89 TGEVDAGRSSFGIEEDPSPIDDGHLTVLQQKEKELIDEVTVRSSEVEHLEQELEMMKAAA 148
TGE G + E + G V++ KEK ++DE+T + LE+E +++K
Sbjct: 495 TGEDFEGLEFDDLLELEKTLPSGLKRVIELKEKRIMDEITAVQKKGIQLEEENKLLKQKM 316
Query: 149 EM 150
M
Sbjct: 315 AM 310
>AW428701
Length = 322
Score = 29.3 bits (64), Expect = 1.2
Identities = 28/92 (30%), Positives = 41/92 (44%), Gaps = 12/92 (13%)
Frame = +1
Query: 119 KEKELIDEVTVRSSEVEHLEQELEMMKAAAE----MAFNNQQSVDSYL-------EQLTE 167
+ K I+E R E+ LE+EL MM++ E Q++ Y E L +
Sbjct: 13 RHKAKIEESQRRQMEMYRLEKELYMMQSLVEGYRKAMKETQKAFAEYRARCPQADEPLYK 192
Query: 168 QILAKGNH-LSTLELEWDAIRKPLEERKKTLE 198
+ G LS ELE + ++K EER K E
Sbjct: 193 DVPGSGGLVLSVTELEKERLKKEEEERAKMKE 288
>TC13083
Length = 528
Score = 28.9 bits (63), Expect = 1.5
Identities = 18/80 (22%), Positives = 37/80 (45%), Gaps = 4/80 (5%)
Frame = +3
Query: 221 EEKSISSEIRKREEEHSKLLADLEKQQKVAS----RKSYTHRIEEITKNSRKQDADIERI 276
++K + E+ + + SKL +LEK+ + R ++ + +S K + D +RI
Sbjct: 75 QDKEFNGEVTRTQRLDSKLPLELEKKMVIRGDTERELDKVRRAKDESSSSCKVELDPKRI 254
Query: 277 LKETREVQLESNSIHERLHR 296
++ R S ++ HR
Sbjct: 255 IRRARSKSEHSMKREDKDHR 314
>TC14457 similar to UP|Q8GTZ1 (Q8GTZ1) bZIP transcription factor, partial
(50%)
Length = 645
Score = 28.9 bits (63), Expect = 1.5
Identities = 24/103 (23%), Positives = 51/103 (49%)
Frame = +1
Query: 209 HVMLQKLREMQQEEKSISSEIRKREEEHSKLLADLEKQQKVASRKSYTHRIEEITKNSRK 268
HVM QK R+ Q + + R R++EH + ++ +Q K + + T+ I T+
Sbjct: 4 HVMDQKKRKRMQSNRESARRSRMRKQEHLEGMSAQVEQLKKENNQISTN-IGVTTQMYLN 180
Query: 269 QDADIERILKETREVQLESNSIHERLHRTYAVADEMVFREAKK 311
+A+ + + E+ S++E +H + ++ +F EA++
Sbjct: 181 VEAENAILRVQMAELSNRLQSLNEIIHYIES-SNNYLFHEAQE 306
>TC12165 weakly similar to UP|Q9FFY6 (Q9FFY6) Similarity to nascent
polypeptide associated complex alpha chain, partial
(32%)
Length = 552
Score = 28.5 bits (62), Expect = 2.0
Identities = 29/95 (30%), Positives = 44/95 (45%), Gaps = 5/95 (5%)
Frame = +3
Query: 55 GSTSSDASISQANKDES-----NSVNDELNSVLNVNKLDTGEVDAGRSSFGIEEDPSPID 109
GS S A +S +D S N++N + +V N +V A R + E +
Sbjct: 282 GSVGSPARVSSQTEDPSEVCKRNAINKLVETVHN-------DVTALRKTRESEMEGLFSW 440
Query: 110 DGHLTVLQQKEKELIDEVTVRSSEVEHLEQELEMM 144
G VL+Q+E L V E+E LEQ+L+M+
Sbjct: 441 QG---VLKQREDHLNRGVREMQGEIEGLEQQLQMV 536
>AV777131
Length = 408
Score = 28.1 bits (61), Expect = 2.6
Identities = 28/95 (29%), Positives = 41/95 (42%), Gaps = 4/95 (4%)
Frame = +2
Query: 182 EWDA----IRKPLEERKKTLEESLYSSSPDAHVMLQKLREMQQEEKSISSEIRKREEEHS 237
EW A RK E+ +T ++ LY D+ + SS R+++ S
Sbjct: 86 EWIAGEYYYRKRKEKHAQTKDDVLYGVFADS-------------DDDYSSRKRRKDRSMS 226
Query: 238 KLLADLEKQQKVASRKSYTHRIEEITKNSRKQDAD 272
K DL K S ++ EEI KNSR++D D
Sbjct: 227 KK-QDLTKPVNFVSTGTFMPN-EEIDKNSREKDED 325
>TC17313 similar to UP|Q9FH68 (Q9FH68) Genomic DNA, chromosome 5, TAC
clone:K16E1, partial (73%)
Length = 959
Score = 27.3 bits (59), Expect = 4.4
Identities = 17/55 (30%), Positives = 29/55 (51%), Gaps = 4/55 (7%)
Frame = +3
Query: 141 LEMMKAAAEMAFNNQQS----VDSYLEQLTEQILAKGNHLSTLELEWDAIRKPLE 191
L M+K A + + + Q + S +++L + KGN TLE E +A++K E
Sbjct: 477 LMMIKMAMQRSIRHWQKKSWHLKSKVKKLESECEVKGNKAKTLETEVEALKKQSE 641
>TC15421 similar to GB|AAL36081.1|17380636|AY063731 AT3g49800/T16K5_150
{Arabidopsis thaliana;}, partial (44%)
Length = 856
Score = 26.9 bits (58), Expect = 5.8
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 11/73 (15%)
Frame = +2
Query: 208 AHVMLQKLREMQQEEKSISSEIRKREEEHSKLLADLEKQ--QKVASRKSYTHR------- 258
A + LR ++EE+ + +++E+H+ ADL+ Q AS + TH
Sbjct: 26 AKTIANSLRLDEEEEEEEEEQDEQQDEQHNNNGADLKSQSDSPSASASASTHSPTARGVK 205
Query: 259 --IEEITKNSRKQ 269
I E+TK+ +Q
Sbjct: 206 EDISELTKSFSRQ 244
>TC14495 UP|O22618 (O22618) Aspartate aminotransferase , complete
Length = 2030
Score = 26.6 bits (57), Expect = 7.5
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -1
Query: 211 MLQKLREMQQEEKSISSEIRK 231
MLQKLRE+ Q E S+ +I K
Sbjct: 287 MLQKLRELDQVEPSVFPQIEK 225
>CB827458
Length = 566
Score = 26.6 bits (57), Expect = 7.5
Identities = 15/58 (25%), Positives = 30/58 (50%)
Frame = +3
Query: 182 EWDAIRKPLEERKKTLEESLYSSSPDAHVMLQKLREMQQEEKSISSEIRKREEEHSKL 239
E D + L+ + +L+ +L +++ + H Q + E S+S+ +RK + SKL
Sbjct: 210 EKDHLIAELQSQLDSLDATLSATADNLHRAEQDKESLINENASLSNTVRKLNRDVSKL 383
>TC18924 homologue to UP|Q94FN3 (Q94FN3) Phosphatidylinositol transfer-like
protein II, complete
Length = 1976
Score = 26.2 bits (56), Expect = 9.8
Identities = 14/51 (27%), Positives = 29/51 (56%)
Frame = +3
Query: 114 TVLQQKEKELIDEVTVRSSEVEHLEQELEMMKAAAEMAFNNQQSVDSYLEQ 164
T + +++E+++ R+ + LEQEL K A E + Q+ + +Y+E+
Sbjct: 1515 TCMPPEKEEMLNAAISRA---DALEQELMSTKKALEDSLAKQEELSAYIEK 1658
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.128 0.331
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,861,363
Number of Sequences: 28460
Number of extensions: 35919
Number of successful extensions: 180
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of query: 387
length of database: 4,897,600
effective HSP length: 92
effective length of query: 295
effective length of database: 2,279,280
effective search space: 672387600
effective search space used: 672387600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146751.14