BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146747.9 - phase: 2 /pseudo
(441 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW720177 134 3e-32
TC16884 weakly similar to UP|Q9FI58 (Q9FI58) Genomic DNA, chromo... 80 5e-16
TC8424 weakly similar to GB|BAC22265.1|24414015|AP003803 arm rep... 63 8e-11
AV766757 56 1e-08
AV420018 52 3e-07
TC18540 similar to UP|Q944I3 (Q944I3) AT3g01400/T13O15_4, partia... 46 1e-05
AV426171 45 2e-05
AV407847 36 0.014
AV773479 35 0.025
TC8489 similar to UP|Q9FSF7 (Q9FSF7) O-linked GlcNAc transferase... 31 0.36
TC17548 31 0.46
BP059692 31 0.46
TC8936 similar to UP|Q84TU4 (Q84TU4) Arm repeat-containing prote... 30 0.79
TC18874 similar to UP|Q9FJJ0 (Q9FJJ0) Genomic DNA, chromosome 5,... 30 0.79
TC9250 UP|Q9FLP6 (Q9FLP6) Ubiquitin-like protein SMT3-like (AT5g... 29 1.4
AV413565 29 1.8
TC8341 similar to UP|Q94FX1 (Q94FX1) Heme oxygenase 1 (Fragment)... 29 1.8
TC11854 similar to UP|Q84TU4 (Q84TU4) Arm repeat-containing prot... 28 2.3
TC15229 weakly similar to UP|O60585 (O60585) Ser/Arg-related nuc... 28 3.0
BP073752 28 3.9
>AW720177
Length = 505
Score = 134 bits (337), Expect = 3e-32
Identities = 69/107 (64%), Positives = 81/107 (75%), Gaps = 1/107 (0%)
Frame = +3
Query: 1 WSIFGVKLTQLQTHLTDFSTEYSKSSTTNPLSLHLLHSVLQTLNDAVSLSHHCQSQI-LP 59
WS+ VKLT LQTHLTDFS E+ SS TNPLSL L HS+ TL+ AVSL+ C S LP
Sbjct: 177 WSLVRVKLTNLQTHLTDFSAEFPSSSATNPLSLDLFHSLSATLHSAVSLAQQCHSPTHLP 356
Query: 60 HGKLQTQSQIDSLIATLHRHVTDCDVLFRSGLLLETPAFSKREAVRS 106
GKLQTQS +DSL+ATL H++ CD+L RSGLLL+ PA SKR+AVRS
Sbjct: 357 RGKLQTQSDLDSLLATLDCHLSHCDILLRSGLLLDAPATSKRDAVRS 497
>TC16884 weakly similar to UP|Q9FI58 (Q9FI58) Genomic DNA, chromosome 5, TAC
clone:K3K7, partial (24%)
Length = 682
Score = 80.5 bits (197), Expect = 5e-16
Identities = 40/48 (83%), Positives = 44/48 (91%)
Frame = +1
Query: 370 LTVRIAAARAVYAMGLNGGNKTRKEMGECGCVPSLIKMLDGKGVEEKD 417
L VRIAAARAV+A+GL+ G KTRKEMGECGCVP LIKMLDGKGVEEK+
Sbjct: 1 LAVRIAAARAVHALGLSSG-KTRKEMGECGCVPGLIKMLDGKGVEEKE 141
Score = 31.2 bits (69), Expect = 0.36
Identities = 16/35 (45%), Positives = 18/35 (50%)
Frame = +2
Query: 406 KMLDGKGVEEKDLLQWRCRCCCSTLSIGGSLERMR 440
K L GK + K LLQW CR C T G RM+
Sbjct: 107 KCLMGKVWKRKKLLQWHCRFC*FTRGTEGCFVRMK 211
>TC8424 weakly similar to GB|BAC22265.1|24414015|AP003803 arm repeat
containing protein homolog-like {Oryza sativa (japonica
cultivar-group);} , partial (49%)
Length = 1586
Score = 63.2 bits (152), Expect = 8e-11
Identities = 51/187 (27%), Positives = 96/187 (51%), Gaps = 3/187 (1%)
Frame = +3
Query: 109 RNLIARLQIGSPESRATAIDSLLSLLNQDDKNVTIAVAQGVVPVLVRLLDSSSDMKEKTV 168
++LI L+ G+ S+ A +LLSL ++ +I + + P++ L++ SS K+ +
Sbjct: 489 KSLIYVLKTGTETSKQNAACALLSLALVEENRSSIGASGAIPPLVSLLINGSSRGKKDAL 668
Query: 169 AAISRVSTVESGKNNLLAEGLLLLNHLVR-VLDSGSGLAIEKACIALQALSLSRDNARAI 227
+ ++ +V+ K + G++ LV V + G+GLA EKA + L +L+ +D AI
Sbjct: 669 TTLYKLCSVKLNKERAVNAGVV--KPLVDLVAEQGTGLA-EKAMVVLNSLAAVQDGKDAI 839
Query: 228 GSRGGISSLLGICQGGTPGSQGYAAAVLRNLAKFNEIKEN--FVEENAVIVLLGLASSGT 285
GGI++L+ + G+ + +A L L + ++ V E + L+ L+ SGT
Sbjct: 840 VEEGGIAALVEAIEDGSVKGKEFAVLTLLQLC-VDSVRNRGLLVREGGIPPLVSLSQSGT 1016
Query: 286 GLARENA 292
A+ A
Sbjct: 1017PRAKHKA 1037
Score = 51.6 bits (122), Expect = 3e-07
Identities = 45/150 (30%), Positives = 75/150 (50%), Gaps = 2/150 (1%)
Frame = +3
Query: 111 LIARLQIGSPESRATAIDSLLSLLNQDDKNVTIAVAQGVVPVLVRLL-DSSSDMKEKTVA 169
L++ L GS + A+ +L L + N AV GVV LV L+ + + + EK +
Sbjct: 618 LVSLLINGSSRGKKDALTTLYKLCSVK-LNKERAVNAGVVKPLVDLVAEQGTGLAEKAMV 794
Query: 170 AISRVSTVESGKNNLLAEGLLLLNHLVRVLDSGSGLAIEKACIALQALSLSRDNARAIGS 229
++ ++ V+ GK+ ++ EG + LV ++ GS E A + L L + R +
Sbjct: 795 VLNSLAAVQDGKDAIVEEGGIAA--LVEAIEDGSVKGKEFAVLTLLQLCVDSVRNRGLLV 968
Query: 230 R-GGISSLLGICQGGTPGSQGYAAAVLRNL 258
R GGI L+ + Q GTP ++ A +LR L
Sbjct: 969 REGGIPPLVSLSQSGTPRAKHKAETLLRYL 1058
>AV766757
Length = 601
Score = 55.8 bits (133), Expect = 1e-08
Identities = 44/146 (30%), Positives = 73/146 (49%), Gaps = 1/146 (0%)
Frame = +2
Query: 115 LQIGSPESRATAIDSLLSLLNQDDKNVTIAVAQGVVPVLVRLLDSSSDMKEKTVA-AISR 173
L+ GS E+R A +L SL D+ VTI + G +P LV LL + +K A A+
Sbjct: 134 LRKGSMEARENAAATLFSLSVVDENKVTIG-SSGAIPPLVTLLSEGTQRGKKDAATALFN 310
Query: 174 VSTVESGKNNLLAEGLLLLNHLVRVLDSGSGLAIEKACIALQALSLSRDNARAIGSRGGI 233
+ + K + G++ L+++L SG +++A L LS D AIG+ +
Sbjct: 311 LCIYQGNKGKAVRAGVIPT--LMKLLTEPSGGMVDEALAILAILSSHPDGKAAIGAADAV 484
Query: 234 SSLLGICQGGTPGSQGYAAAVLRNLA 259
L+ G+P ++ +AAVL +L+
Sbjct: 485 PILVEFIGNGSPRNKENSAAVLVHLS 562
>AV420018
Length = 213
Score = 51.6 bits (122), Expect = 3e-07
Identities = 25/67 (37%), Positives = 38/67 (56%)
Frame = +1
Query: 218 SLSRDNARAIGSRGGISSLLGICQGGTPGSQGYAAAVLRNLAKFNEIKENFVEENAVIVL 277
S+S D RAI GG+ L+ +CQ G SQ AA L+N++ E+++ EE V V+
Sbjct: 4 SMSADTTRAIVGHGGVPPLIELCQSGESVSQAAAACALKNISSVPEVRQVLAEEGVVKVM 183
Query: 278 LGLASSG 284
+ L + G
Sbjct: 184 INLLNFG 204
>TC18540 similar to UP|Q944I3 (Q944I3) AT3g01400/T13O15_4, partial (40%)
Length = 433
Score = 46.2 bits (108), Expect = 1e-05
Identities = 40/126 (31%), Positives = 60/126 (46%), Gaps = 2/126 (1%)
Frame = +3
Query: 157 LDSSSDMK--EKTVAAISRVSTVESGKNNLLAEGLLLLNHLVRVLDSGSGLAIEKACIAL 214
L SS D++ E V AI +S + K + + G + LVR L +G+ A E A AL
Sbjct: 9 LISSQDLQLQEYGVTAILNLSLCDENKELIASSGAI--KPLVRALKAGTPTAKENAACAL 182
Query: 215 QALSLSRDNARAIGSRGGISSLLGICQGGTPGSQGYAAAVLRNLAKFNEIKENFVEENAV 274
LS + ++ AIG G I L+ + Q G + A+ L +L E K V+ +
Sbjct: 183 LRLSQAEESKAAIGRSGAIPLLVSLLQTGGIRGKKDASTALYSLCSVRENKIRAVKAGIM 362
Query: 275 IVLLGL 280
VL+ L
Sbjct: 363 KVLVEL 380
Score = 43.5 bits (101), Expect = 7e-05
Identities = 33/112 (29%), Positives = 55/112 (48%)
Frame = +3
Query: 208 EKACIALQALSLSRDNARAIGSRGGISSLLGICQGGTPGSQGYAAAVLRNLAKFNEIKEN 267
E A+ LSL +N I S G I L+ + GTP ++ AA L L++ E K
Sbjct: 39 EYGVTAILNLSLCDENKELIASSGAIKPLVRALKAGTPTAKENAACALLRLSQAEESKAA 218
Query: 268 FVEENAVIVLLGLASSGTGLARENAIGCVANLISEDESMRVLAVKEGGVECL 319
A+ +L+ L +G +++A + +L S E+ ++ AVK G ++ L
Sbjct: 219 IGRSGAIPLLVSLLQTGGIRGKKDASTALYSLCSVREN-KIRAVKAGIMKVL 371
>AV426171
Length = 283
Score = 45.4 bits (106), Expect = 2e-05
Identities = 28/88 (31%), Positives = 51/88 (57%)
Frame = +2
Query: 162 DMKEKTVAAISRVSTVESGKNNLLAEGLLLLNHLVRVLDSGSGLAIEKACIALQALSLSR 221
+++E++ ++ V+ +S K L+ G++ L+RVLD GS L + L L+ +
Sbjct: 23 EIQEESAKVVAVVAGFDSYKGVLVGAGVIA--PLIRVLDCGSELGKVASARCLMKLTENS 196
Query: 222 DNARAIGSRGGISSLLGICQGGTPGSQG 249
DNA + ++GG+++LL IC G S+G
Sbjct: 197 DNAWCVSAQGGVTALLNIC--GDADSKG 274
Score = 31.2 bits (69), Expect = 0.36
Identities = 19/60 (31%), Positives = 30/60 (49%), Gaps = 3/60 (5%)
Frame = +2
Query: 333 EVGVEMLRYLAMTGPIDE---VLVGEGFVGRVIGVLDCDVLTVRIAAARAVYAMGLNGGN 389
E+ E + +A+ D VLVG G + +I VLDC ++A+AR + + N N
Sbjct: 23 EIQEESAKVVAVVAGFDSYKGVLVGAGVIAPLIRVLDCGSELGKVASARCLMKLTENSDN 202
>AV407847
Length = 423
Score = 35.8 bits (81), Expect = 0.014
Identities = 27/105 (25%), Positives = 47/105 (44%), Gaps = 3/105 (2%)
Frame = +1
Query: 102 EAVRSLTRNLIARLQIGSPESRATAIDSLLSLLNQDDKNVTIAVAQGVVPVLVRLLDSSS 161
EA + ++ L+ +L + + + L L D + G +PVLVR L + S
Sbjct: 10 EATKMMSTFLVNKLNVSDLDESNAVVYELRVLAKTDSDSRACIAEAGAIPVLVRYLTTGS 189
Query: 162 ---DMKEKTVAAISRVSTVESGKNNLLAEGLLLLNHLVRVLDSGS 203
++ V I +S +E+ K ++ E LN + VL SG+
Sbjct: 190 HHPSLQVNAVTTILNLSILEANKTRIM-ETDGALNGVAEVLRSGA 321
>AV773479
Length = 483
Score = 35.0 bits (79), Expect = 0.025
Identities = 23/76 (30%), Positives = 37/76 (48%), Gaps = 2/76 (2%)
Frame = +2
Query: 227 IGSRGGISSLLGICQGGTPGSQ--GYAAAVLRNLAKFNEIKENFVEENAVIVLLGLASSG 284
+ ++GG+++LL IC G A V RNL EIK +EE V L GL +
Sbjct: 47 VSAQGGVTALLNICGDADSKGDWVGPACGV*RNLLGVEEIKRFMMEEGVVPRLSGL*NQR 226
Query: 285 TGLARENAIGCVANLI 300
+ N+I + +++
Sbjct: 227 RKQCQVNSIAFIQSIV 274
>TC8489 similar to UP|Q9FSF7 (Q9FSF7) O-linked GlcNAc transferase like
(Fragment), partial (53%)
Length = 606
Score = 31.2 bits (69), Expect = 0.36
Identities = 16/46 (34%), Positives = 24/46 (51%), Gaps = 2/46 (4%)
Frame = +2
Query: 374 IAAARAVYA--MGLNGGNKTRKEMGECGCVPSLIKMLDGKGVEEKD 417
+ A+ YA + L GG TR G C C ++ ++ GK EEK+
Sbjct: 104 LQTAKKYYASTIDLTGGKNTRALFGICLCTSAIAQLSKGKSKEEKE 241
>TC17548
Length = 502
Score = 30.8 bits (68), Expect = 0.46
Identities = 38/146 (26%), Positives = 62/146 (42%)
Frame = +2
Query: 195 LVRVLDSGSGLAIEKACIALQALSLSRDNARAIGSRGGISSLLGICQGGTPGSQGYAAAV 254
L+++LDS +G A AL L+ + DN GG+ L Q G Q V
Sbjct: 41 LLKLLDSKNGSLQHNAAFALYGLADNEDNVSDFIRVGGVQRL----QEGEFIVQATKDCV 208
Query: 255 LRNLAKFNEIKENFVEENAVIVLLGLASSGTGLARENAIGCVANLISEDESMRVLAVKEG 314
+ L + E + V + +L + S G R A+ +A+L S D+ R+ +
Sbjct: 209 AKTLKRLEEKIHDRVLSH---LLYLMRVSEKGFQRRVAL-ALAHLCSADDQRRIF-IDNH 373
Query: 315 GVECLKNYWDSVTMIQSLEVGVEMLR 340
G+E L S + Q L+ V + +
Sbjct: 374 GLELLIGLLGSSSSKQQLDGAVALCK 451
>BP059692
Length = 486
Score = 30.8 bits (68), Expect = 0.46
Identities = 27/87 (31%), Positives = 40/87 (45%)
Frame = -1
Query: 222 DNARAIGSRGGISSLLGICQGGTPGSQGYAAAVLRNLAKFNEIKENFVEENAVIVLLGLA 281
+NA AI + +SSL + GG P S+ E+ E FV++ + +L
Sbjct: 204 NNADAIKGKYDLSSLHTVLSGGAPLSK--------------EVTEGFVDKYPNVTIL--- 76
Query: 282 SSGTGLARENAIGCVANLISEDESMRV 308
G GL + +G A+ S DES RV
Sbjct: 75 -QGYGLTESSGVG--ASTDSLDESRRV 4
>TC8936 similar to UP|Q84TU4 (Q84TU4) Arm repeat-containing protein,
partial (9%)
Length = 561
Score = 30.0 bits (66), Expect = 0.79
Identities = 17/51 (33%), Positives = 25/51 (48%)
Frame = +2
Query: 242 GGTPGSQGYAAAVLRNLAKFNEIKENFVEENAVIVLLGLASSGTGLARENA 292
G G + AAA+L N +++ AV L+ L+ SGT A+E A
Sbjct: 11 GSARGKENAAAALLHLCLHSNRFLSTVLQQGAVPPLVALSQSGTPRAKEKA 163
>TC18874 similar to UP|Q9FJJ0 (Q9FJJ0) Genomic DNA, chromosome 5, TAC
clone:K19B1, partial (33%)
Length = 543
Score = 30.0 bits (66), Expect = 0.79
Identities = 36/129 (27%), Positives = 60/129 (45%)
Frame = +2
Query: 191 LLNHLVRVLDSGSGLAIEKACIALQALSLSRDNARAIGSRGGISSLLGICQGGTPGSQGY 250
LL+ L +++S + A +L LSL + N I G + L+ + +GG SQ +
Sbjct: 44 LLSALRLLVESRYVVVQVNAVASLVNLSLEKTNKVRIVRSGFVPFLIDVLKGGFSESQEH 223
Query: 251 AAAVLRNLAKFNEIKENFVEENAVIVLLGLASSGTGLARENAIGCVANLISEDESMRVLA 310
AA L +LA ++ K A+ L+ S + R ++ + +L + +S RV
Sbjct: 224 AAGALFSLALDDDNKMAIGVLGALQPLMHALRSESERTRHDSALALYHL-TLVQSNRVKL 400
Query: 311 VKEGGVECL 319
VK G V L
Sbjct: 401 VKLGVVPTL 427
>TC9250 UP|Q9FLP6 (Q9FLP6) Ubiquitin-like protein SMT3-like
(AT5g55160/MCO15_11) (Small ubiquitin-like modifier 2),
partial (22%)
Length = 1363
Score = 29.3 bits (64), Expect = 1.4
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = -3
Query: 25 SSTTNPLSLHLLHSVLQTLNDAVSLSHHCQSQILPHGKLQT 65
S T LSL L QTL++A++LS H PH + T
Sbjct: 1115 SKFTKCLSLQALLQTRQTLSEAINLSAHFLQNTCPHELMAT 993
>AV413565
Length = 298
Score = 28.9 bits (63), Expect = 1.8
Identities = 15/31 (48%), Positives = 18/31 (57%), Gaps = 2/31 (6%)
Frame = -1
Query: 31 LSLHLLHSVLQTLNDAVSLS--HHCQSQILP 59
LSLH+L +Q A SL HH Q Q+LP
Sbjct: 109 LSLHILELSIQKFQQAWSLQIFHHLQGQVLP 17
>TC8341 similar to UP|Q94FX1 (Q94FX1) Heme oxygenase 1 (Fragment), partial
(86%)
Length = 1286
Score = 28.9 bits (63), Expect = 1.8
Identities = 10/18 (55%), Positives = 13/18 (71%)
Frame = -3
Query: 421 WRCRCCCSTLSIGGSLER 438
WR RCCC+ L +G +ER
Sbjct: 384 WRKRCCCNNLVVGK*IER 331
>TC11854 similar to UP|Q84TU4 (Q84TU4) Arm repeat-containing protein,
partial (9%)
Length = 615
Score = 28.5 bits (62), Expect = 2.3
Identities = 20/69 (28%), Positives = 31/69 (43%), Gaps = 1/69 (1%)
Frame = +3
Query: 196 VRVLDSGSGLAIEKA-CIALQALSLSRDNARAIGSRGGISSLLGICQGGTPGSQGYAAAV 254
V +++SGS E A CI LQ S + G + L+ + Q GTP ++ A +
Sbjct: 3 VEIVESGSQRGKENAACILLQLCLHSYKLCTLVLPEGAVPPLVALSQSGTPRAREKAPQL 182
Query: 255 LRNLAKFNE 263
L + E
Sbjct: 183 LSHFRNQRE 209
>TC15229 weakly similar to UP|O60585 (O60585) Ser/Arg-related nuclear matrix
protein, partial (4%)
Length = 965
Score = 28.1 bits (61), Expect = 3.0
Identities = 26/83 (31%), Positives = 41/83 (49%), Gaps = 2/83 (2%)
Frame = +3
Query: 91 LLLETPAFSKREAVRSLTRNLIARLQIGSPESRAT--AIDSLLSLLNQDDKNVTIAVAQG 148
LL PA A +LT ++ ++ I RAT A +LLSL ++ ++ AVA G
Sbjct: 369 LLCRVPAGCAEFAGHALTVPMLVKI-ILKISDRATEYAAGALLSLCSESERLQMEAVAAG 545
Query: 149 VVPVLVRLLDSSSDMKEKTVAAI 171
V+ L+ L+ S + K A +
Sbjct: 546 VLTQLLLLVQSDCTERAKRKAQL 614
>BP073752
Length = 403
Score = 27.7 bits (60), Expect = 3.9
Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +1
Query: 24 KSSTTNPLSLHLLHSVLQTLNDAVSLSHHCQSQILPHGK-LQTQSQIDSLIAT 75
K ++ PLS+ S +Q L D +++S CQ + P K L + ++ LIA+
Sbjct: 136 KINSQFPLSISRSSSQVQNLRDLITISPGCQMKKCPR*KYLNLERRVSILIAS 294
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.134 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,897,679
Number of Sequences: 28460
Number of extensions: 91198
Number of successful extensions: 745
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 736
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 742
length of query: 441
length of database: 4,897,600
effective HSP length: 93
effective length of query: 348
effective length of database: 2,250,820
effective search space: 783285360
effective search space used: 783285360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146747.9