BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146743.16 - phase: 2 /pseudo
(270 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC7962 homologue to UP|Q84V97 (Q84V97) Alcohol dehydrogenase 1 ... 207 2e-54
BP048139 151 1e-40
TC15281 similar to UP|Q8LJR2 (Q8LJR2) Alcohol dehydrogenase 1 (F... 155 9e-39
AU251635 133 3e-32
TC17561 87 4e-18
TC18550 82 1e-16
BP028705 61 2e-10
TC16542 similar to UP|Q93X81 (Q93X81) Sorbitol dehydrogenase, pa... 43 6e-05
CB826968 37 0.004
AV415744 30 0.43
AV407757 30 0.43
TC7966 similar to UP|Q84V25 (Q84V25) Quinone oxidoreductase, par... 29 0.74
TC14844 similar to UP|Q8L553 (Q8L553) SCARECROW transcriptional ... 29 0.74
AV415490 24 1.2
AV765905 28 1.6
TC16413 similar to GB|AAF63224.1|7532410|AC002329 3-hydroxy-3-me... 27 2.8
BP074212 27 3.7
TC13462 27 4.8
TC10352 similar to UP|Q9SK63 (Q9SK63) Expressed protein, partial... 27 4.8
AV764937 27 4.8
>TC7962 homologue to UP|Q84V97 (Q84V97) Alcohol dehydrogenase 1 , complete
Length = 1489
Score = 207 bits (527), Expect = 2e-54
Identities = 106/245 (43%), Positives = 158/245 (64%)
Frame = +1
Query: 26 NGNTRFTDLNGEIIYHFMFISSFSEYTVVDIANVLKIDPQIPPHRACLLGCGVSTGVGAA 85
+ +RF+ + G+ IYHF+ S+FSEYTV+ V KI+P P + C+L CG+ TG GA
Sbjct: 496 DNQSRFS-IKGKPIYHFVGTSTFSEYTVLHAGCVAKINPAAPLDKVCILSCGICTGFGAT 672
Query: 86 WRTAGVEPGSSVAIFGLGSVGLAVAEGARLCGATRIIGVDVNHEKFEIGKKFGLTDFVHG 145
A +PGSSVAIFGLG+VGLA AEGAR+ GA+RIIGVD+ +FE KKFG+ +FV+
Sbjct: 673 VNVAKPKPGSSVAIFGLGAVGLAAAEGARVSGASRIIGVDLVSSRFEGAKKFGVNEFVNP 852
Query: 146 EECGNKSVSQIIIEMTDGGADYCFECVGMASLVHEAYASCRKGWGKTIVLGVDKPGARLS 205
++ +K V ++I EMT+GG D EC G + A+ GWG +++GV
Sbjct: 853 KD-HDKPVQEVIAEMTNGGVDRAVECTGSIQAMISAFECVHDGWGVAVLVGVPNQDDAFQ 1029
Query: 206 LSSSEVLHDGKSLMGSVYGGLKPKSHVPILLKRYMDKELQLDEFVTHELEFKDINKAFDL 265
L++ ++L G+ YG KP++ +P +++ YM EL+L++F+TH + F +IN+AFD
Sbjct: 1030THPVNFLNE-RTLKGTFYGNYKPRTDLPNVVEMYMRGELELEKFITHTVSFSEINQAFDY 1206
Query: 266 LSKGD 270
+ KG+
Sbjct: 1207MLKGE 1221
>BP048139
Length = 567
Score = 151 bits (381), Expect(2) = 1e-40
Identities = 70/82 (85%), Positives = 77/82 (93%)
Frame = -2
Query: 23 LPRNGNTRFTDLNGEIIYHFMFISSFSEYTVVDIANVLKIDPQIPPHRACLLGCGVSTGV 82
+PR+G TRFTDLNGEII+HFMFISSFSEYTVVD+ANV KIDP+IPP RACLL CG+STGV
Sbjct: 248 MPRDGTTRFTDLNGEIIHHFMFISSFSEYTVVDVANVTKIDPEIPPDRACLLSCGISTGV 69
Query: 83 GAAWRTAGVEPGSSVAIFGLGS 104
GAAWRTA VEPGS+VAIFGLGS
Sbjct: 68 GAAWRTASVEPGSTVAIFGLGS 3
Score = 31.6 bits (70), Expect(2) = 1e-40
Identities = 15/19 (78%), Positives = 16/19 (83%)
Frame = -3
Query: 3 IANQPRATVVLISHSKFLL 21
IANQPRA VLISHS+ LL
Sbjct: 307 IANQPRAIAVLISHSRCLL 251
>TC15281 similar to UP|Q8LJR2 (Q8LJR2) Alcohol dehydrogenase 1 (Fragment),
partial (50%)
Length = 785
Score = 155 bits (391), Expect = 9e-39
Identities = 77/175 (44%), Positives = 116/175 (66%)
Frame = +1
Query: 96 SVAIFGLGSVGLAVAEGARLCGATRIIGVDVNHEKFEIGKKFGLTDFVHGEECGNKSVSQ 155
+VA+FGLG+VGLA AEGARL GA+RIIGVD+ +FE KKFG+TDFV+ ++ +K V Q
Sbjct: 1 TVAVFGLGAVGLAAAEGARLSGASRIIGVDLVAGRFEQAKKFGVTDFVNPKD-HDKPVQQ 177
Query: 156 IIIEMTDGGADYCFECVGMASLVHEAYASCRKGWGKTIVLGVDKPGARLSLSSSEVLHDG 215
+I+EMT+G D EC G A+ GWG +++GV A + +G
Sbjct: 178 VIVEMTNG*VDRAIECTGNIQATISAFECLHDGWGVAVIVGVPTKDAEFKTHPIHFM-EG 354
Query: 216 KSLMGSVYGGLKPKSHVPILLKRYMDKELQLDEFVTHELEFKDINKAFDLLSKGD 270
++L G+ YG + +S +P L+++YM+KEL+L++F+TH + F +IN+AF + KG+
Sbjct: 355 RTLKGTFYGNFQTRSDIPSLVEKYMNKELELEKFITHSVPFSEINQAFAYMLKGE 519
>AU251635
Length = 383
Score = 133 bits (335), Expect = 3e-32
Identities = 60/115 (52%), Positives = 85/115 (73%)
Frame = +3
Query: 91 VEPGSSVAIFGLGSVGLAVAEGARLCGATRIIGVDVNHEKFEIGKKFGLTDFVHGEECGN 150
VEPGS VA+FGLG+VGLAVAEGA+ GA+RIIG+D++ KFE K FG+T+F++ +E
Sbjct: 6 VEPGSIVAVFGLGTVGLAVAEGAKAAGASRIIGIDIDSNKFERAKNFGVTEFINPKE-HE 182
Query: 151 KSVSQIIIEMTDGGADYCFECVGMASLVHEAYASCRKGWGKTIVLGVDKPGARLS 205
K + Q+I+++TDGG DY FEC+G S++ A C KGWG ++++GV G +S
Sbjct: 183 KPIQQVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAASGQEIS 347
>TC17561
Length = 581
Score = 86.7 bits (213), Expect = 4e-18
Identities = 49/136 (36%), Positives = 82/136 (60%), Gaps = 2/136 (1%)
Frame = +3
Query: 137 FGLTDFVHGEECGNKSVSQIIIEMTDG-GADYCFECVGMASLVHEAYASCRKGWGKTIVL 195
FG+T F++ + G KS+S+++ E++ G G DY EC G L+ E+ A+ + G GKTI +
Sbjct: 6 FGMTHFINPGDSG-KSLSELVKELSGGVGVDYSIECTGAPPLLTESVAATKVGTGKTIAV 182
Query: 196 GV-DKPGARLSLSSSEVLHDGKSLMGSVYGGLKPKSHVPILLKRYMDKELQLDEFVTHEL 254
G+ +P L + L G++L GS +GG+K + + I+ + + +E L E THE+
Sbjct: 183 GMAPEPVVPFGLLA---LLFGRTLKGSAFGGIKTTTDLSIIANKCLKQEFPLQELFTHEV 353
Query: 255 EFKDINKAFDLLSKGD 270
D++KAF+LL + D
Sbjct: 354 PLSDVDKAFELLKQPD 401
>TC18550
Length = 688
Score = 81.6 bits (200), Expect = 1e-16
Identities = 51/135 (37%), Positives = 80/135 (58%), Gaps = 2/135 (1%)
Frame = +2
Query: 134 GKKFGLTDFVHGEECGNKSVSQIIIEMTDG-GADYCFECVGMASLVHEAYASCRKGWGKT 192
G+ FG+T ++ + KS S+++ E++ G G DY FEC G A L E+ + + G G+T
Sbjct: 5 GEAFGMTHSINPGD-STKSASELVKELSGGIGMDYSFECSGFAPLPTESLEATKVGTGET 181
Query: 193 IVLGVD-KPGARLSLSSSEVLHDGKSLMGSVYGGLKPKSHVPILLKRYMDKELQLDEFVT 251
I +GV +P ++ S +L+ G++L GS+ GGLK S I+ + +E L E T
Sbjct: 182 IAIGVGTEPIVPFAILS--ILY-GRTLKGSILGGLKAISDFSIIANKCQKEEFPLQELFT 352
Query: 252 HELEFKDINKAFDLL 266
HE+ DI+KAF+LL
Sbjct: 353 HEVTLADISKAFELL 397
>BP028705
Length = 474
Score = 61.2 bits (147), Expect = 2e-10
Identities = 25/56 (44%), Positives = 40/56 (70%)
Frame = -1
Query: 214 DGKSLMGSVYGGLKPKSHVPILLKRYMDKELQLDEFVTHELEFKDINKAFDLLSKG 269
DGK+L G+ +G KPK+ +P ++ +M +L+LD+F+TH + +INKAFD + KG
Sbjct: 441 DGKTLTGTAFGDYKPKTGLPSVVDLFMSNKLELDKFITHRVPLSEINKAFDYMIKG 274
>TC16542 similar to UP|Q93X81 (Q93X81) Sorbitol dehydrogenase, partial (60%)
Length = 724
Score = 42.7 bits (99), Expect = 6e-05
Identities = 24/61 (39%), Positives = 33/61 (53%)
Frame = +1
Query: 78 VSTGVGAAWRTAGVEPGSSVAIFGLGSVGLAVAEGARLCGATRIIGVDVNHEKFEIGKKF 137
+S GV A R A + P ++V I G G +GL AR GA RI+ VDV+ + + K
Sbjct: 538 LSVGVHAC-RRANIGPETNVLIMGAGPIGLVTMLAARAFGAPRIVIVDVDDHRLSVAKTL 714
Query: 138 G 138
G
Sbjct: 715 G 717
>CB826968
Length = 473
Score = 37.0 bits (84), Expect = 0.004
Identities = 13/28 (46%), Positives = 23/28 (81%)
Frame = +1
Query: 242 KELQLDEFVTHELEFKDINKAFDLLSKG 269
+E+++DE++TH L +INKAFD++ +G
Sbjct: 271 QEIKVDEYITHNLTLVEINKAFDIMHEG 354
>AV415744
Length = 415
Score = 30.0 bits (66), Expect = 0.43
Identities = 30/112 (26%), Positives = 50/112 (43%), Gaps = 5/112 (4%)
Frame = +1
Query: 89 AGVEPGSSVAIFGL-GSVGLAVAEGARLCGATRIIGVDVNHEKFEIGKKFGLTDFVHGEE 147
A + G + + G G VGLA + + CGA +I V EK ++ K G+ H +
Sbjct: 37 AQLTSGQVLLVLGAAGGVGLAAVQIGKACGAI-VIAVARGAEKVQLLKSHGVD---HVVD 204
Query: 148 CGNKSVSQIIIEMTDG----GADYCFECVGMASLVHEAYASCRKGWGKTIVL 195
N++V++ + E G D ++ VG L E + WG I++
Sbjct: 205 LTNENVTESVKEFLKARKLKGIDVLYDPVG-GKLTKECLRLLK--WGANILI 351
>AV407757
Length = 430
Score = 30.0 bits (66), Expect = 0.43
Identities = 14/21 (66%), Positives = 16/21 (75%)
Frame = +3
Query: 88 TAGVEPGSSVAIFGLGSVGLA 108
T VEPG +VAIFGLG + LA
Sbjct: 123 TESVEPGYTVAIFGLGFIELA 185
>TC7966 similar to UP|Q84V25 (Q84V25) Quinone oxidoreductase, partial (98%)
Length = 1908
Score = 29.3 bits (64), Expect = 0.74
Identities = 28/114 (24%), Positives = 50/114 (43%), Gaps = 2/114 (1%)
Frame = +2
Query: 27 GNTRFTDLNGEIIYHFMFISSFSEYTVVDIANVLKIDPQIPPHRACLLGCGVSTGVGAAW 86
G+ + D+N + + + S +EYT V+ + + A L + T
Sbjct: 986 GDEVYGDINENALEYPKVVGSLAEYTAVEEKLLAHKPKNLSFVEAASLPLAIETAYEGLE 1165
Query: 87 RTAGVEPGSSVAIF-GLGSVGLAVAEGAR-LCGATRIIGVDVNHEKFEIGKKFG 138
R AG G S+ + G G VG V + A+ + GA+++ + K E+ +K G
Sbjct: 1166 R-AGFSAGKSILVLGGAGGVGTHVIQLAKHVFGASKVAATS-STGKLELLRKLG 1321
>TC14844 similar to UP|Q8L553 (Q8L553) SCARECROW transcriptional
regulator-like, partial (49%)
Length = 1051
Score = 29.3 bits (64), Expect = 0.74
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = -3
Query: 245 QLDEFVTHELEFKDINKAFDLLSK 268
QL EF TH LEFK ++F LL++
Sbjct: 182 QLGEFATHHLEFKPDPESFSLLTQ 111
>AV415490
Length = 377
Score = 24.3 bits (51), Expect(2) = 1.2
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -3
Query: 99 IFGLGSVGLAVAEGARLCGATRI 121
+FGLG EG RLCGAT +
Sbjct: 102 VFGLG------IEGKRLCGATAL 52
Score = 22.7 bits (47), Expect(2) = 1.2
Identities = 13/34 (38%), Positives = 15/34 (43%)
Frame = -1
Query: 64 PQIPPHRACLLGCGVSTGVGAAWRTAGVEPGSSV 97
P PP R C G VS + +A GSSV
Sbjct: 194 PISPPLRVCFPGKSVSGKISTPESSAHSREGSSV 93
>AV765905
Length = 559
Score = 28.1 bits (61), Expect = 1.6
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = +3
Query: 188 GWGKTIVLGVDKPGARLSLSSSEVLHDGK 216
GWG I+ D+P L SS+ L +GK
Sbjct: 444 GWGHAILEDRDRPAMNLHFSSTYALQEGK 530
>TC16413 similar to GB|AAF63224.1|7532410|AC002329
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
{Arabidopsis thaliana;}, partial (41%)
Length = 674
Score = 27.3 bits (59), Expect = 2.8
Identities = 14/55 (25%), Positives = 25/55 (45%), Gaps = 4/55 (7%)
Frame = +2
Query: 60 LKIDPQIPPHRACLLGCGVST----GVGAAWRTAGVEPGSSVAIFGLGSVGLAVA 110
L++DPQ+ P C+ W AG+ + + G+ SVGL+++
Sbjct: 320 LRVDPQVHPGEICISHISGQNPNKLSKNPLWNCAGIAAIEVMKMLGIRSVGLSLS 484
>BP074212
Length = 433
Score = 26.9 bits (58), Expect = 3.7
Identities = 20/59 (33%), Positives = 25/59 (41%)
Frame = +2
Query: 88 TAGVEPGSSVAIFGLGSVGLAVAEGARLCGATRIIGVDVNHEKFEIGKKFGLTDFVHGE 146
T + S ++ GL VG EGA G VD H F + KKF L HG+
Sbjct: 188 TRDINSYESTSVRGLYPVG----EGAGYAGGIISAAVDGMHAGFAVAKKFNL---FHGD 343
>TC13462
Length = 625
Score = 26.6 bits (57), Expect = 4.8
Identities = 17/78 (21%), Positives = 33/78 (41%), Gaps = 3/78 (3%)
Frame = +3
Query: 117 GATRIIGVDVNHEKFEIGKKFGLTDFVHGEECGNKSVS---QIIIEMTDGGADYCFECVG 173
GA +I + +FE G V GEE G +V ++ + + G++ + +
Sbjct: 369 GAAAMIAAEEYARRFESGDVLDTPGSVTGEELGQSNVKVFCRMCNRVENEGSERAKKMLS 548
Query: 174 MASLVHEAYASCRKGWGK 191
S + + +C K W +
Sbjct: 549 CKSCSKKYHRTCLKSWAQ 602
>TC10352 similar to UP|Q9SK63 (Q9SK63) Expressed protein, partial (77%)
Length = 631
Score = 26.6 bits (57), Expect = 4.8
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = -2
Query: 222 VYGGLKPKSHVPILLKRYMDKEL 244
V G + PK +P+L+ RYM++ +
Sbjct: 615 VVGDINPKRSMPLLIPRYMEESI 547
>AV764937
Length = 392
Score = 26.6 bits (57), Expect = 4.8
Identities = 24/93 (25%), Positives = 37/93 (38%), Gaps = 2/93 (2%)
Frame = +1
Query: 53 VVDIANVLKIDPQIPPHRACLLGC-GVSTGVGAAWRTAGVEPGSSVAIFGLGSVG-LAVA 110
V D V+ I +P A L C G++ PG + + GLG +G +AV
Sbjct: 7 VADYRYVVHIPENLPMDAAAPLLCAGITVFSPLKDHDLVSSPGKKIGVVGLGGLGHIAVK 186
Query: 111 EGARLCGATRIIGVDVNHEKFEIGKKFGLTDFV 143
G +I + E E ++ G DF+
Sbjct: 187 FGKAFGHHVTVISTSPSKEA-EAKQRLGADDFI 282
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.141 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,720,505
Number of Sequences: 28460
Number of extensions: 66085
Number of successful extensions: 318
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 312
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 312
length of query: 270
length of database: 4,897,600
effective HSP length: 89
effective length of query: 181
effective length of database: 2,364,660
effective search space: 428003460
effective search space used: 428003460
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146743.16