BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146721.2 - phase: 1 /pseudo
(407 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC20160 99 1e-21
TC13610 weakly similar to UP|Q9LSB0 (Q9LSB0) Emb|CAB70981.1, par... 84 4e-17
AV415989 50 7e-07
BP057557 38 0.003
TC14217 33 0.086
TC17801 weakly similar to GB|AAO42978.1|28416895|BT004732 At3g13... 31 0.43
TC15938 similar to UP|O81340 (O81340) 26S proteasome regulatory ... 31 0.43
TC15092 similar to UP|Q8QRX5 (Q8QRX5) UL145, partial (8%) 30 0.95
TC8528 weakly similar to GB|AAO42978.1|28416895|BT004732 At3g139... 29 1.6
AV421856 28 2.1
BP072468 28 3.6
BP064496 28 3.6
AW720152 27 4.7
BG662174 27 4.7
TC18970 weakly similar to UP|Q8I8F0 (Q8I8F0) Chromosome scaffold... 27 6.2
TC9315 27 6.2
TC19530 27 6.2
BP075633 27 6.2
AV410313 27 8.0
BP046365 27 8.0
>TC20160
Length = 466
Score = 99.4 bits (246), Expect = 1e-21
Identities = 51/135 (37%), Positives = 87/135 (63%), Gaps = 2/135 (1%)
Frame = +1
Query: 274 GNNQDKGTPIFLGKNAFSFFIVTDSLSLIASASSVLMFIGILTSRYAEEDFNTSLPAKLL 333
GNN + G+P +L K +F F ++D+ +LI+S++S+L+F+ IL SRYAE+DF SLP KL+
Sbjct: 1 GNNDNTGSPNYLEKPSFLLFALSDATALISSSTSILIFLSILISRYAEDDFLKSLPLKLI 180
Query: 334 FGLFTIFLSVVFMMCSFCSALAL-MLKGYRWIIITAIASSVIPILVFMFSLLRLFSEVCI 392
GL +F+S++ MM +F SA + G +W+ + +PI +F++ L+S++
Sbjct: 181 SGLIALFISIISMMVAFSSAFFITYYHGLKWVPYLISGLAFLPIPLFIYLQFSLWSDIAY 360
Query: 393 S-FLRSYFLRKKRRM 406
S ++ S R ++RM
Sbjct: 361 SAYICSSLFRPRKRM 405
>TC13610 weakly similar to UP|Q9LSB0 (Q9LSB0) Emb|CAB70981.1, partial (7%)
Length = 528
Score = 84.0 bits (206), Expect = 4e-17
Identities = 44/107 (41%), Positives = 72/107 (67%), Gaps = 1/107 (0%)
Frame = +3
Query: 288 NAFSFFIVTDSLSLIASASSVLMFIGILTSRYAEEDFNTSLPAKLLFGLFTIFLSVVFMM 347
N F FIV+D+L+L +S +S+LMF+ IL + YAEEDF +LP +L+ GL ++F ++V M
Sbjct: 6 NTFLVFIVSDALALCSSMASLLMFLAILNAPYAEEDFLNALPHRLIIGLASLFFAIVTTM 185
Query: 348 CSFCSALALML-KGYRWIIITAIASSVIPILVFMFSLLRLFSEVCIS 393
+F +AL+L+L + +W+ I + + +PI +F L LF ++ IS
Sbjct: 186 IAFSAALSLLLQERLKWVPIPIVLLACVPITLFARLQLPLFIQMIIS 326
>AV415989
Length = 281
Score = 50.1 bits (118), Expect = 7e-07
Identities = 27/77 (35%), Positives = 44/77 (57%), Gaps = 1/77 (1%)
Frame = +1
Query: 109 AAKYGIIEFINSMREANPDLLWAMDKYKRGIFAHAILNRQDKVFKLIYEMEGQKE-LKTT 167
AA+ G EF++ + PD++W ++ + I A L+R +F LI+E+ K+ + T
Sbjct: 37 AAQVGNFEFLSVVMSTYPDMIWELNTMDQSIIHIAALHRHASIFNLIHEIGPTKDIILTF 216
Query: 168 KDIFENNLLHLAAELGP 184
D +NNLLH A+L P
Sbjct: 217 IDKKKNNLLHCVAKLAP 267
>BP057557
Length = 497
Score = 38.1 bits (87), Expect = 0.003
Identities = 25/64 (39%), Positives = 32/64 (49%), Gaps = 6/64 (9%)
Frame = -1
Query: 251 SSFTIVGTLIITIMFAAAFTVPGGNNQDKGTPIFLG------KNAFSFFIVTDSLSLIAS 304
+S T+V LI T+ FAA FTVPG + K LG AF F V D L+L S
Sbjct: 362 NSATVVAVLIATVAFAAIFTVPGQYVEAKEQSFSLGPANIANNAAFMIFFVFDILALFIS 183
Query: 305 ASSV 308
+ +
Sbjct: 182 LAVI 171
>TC14217
Length = 985
Score = 33.1 bits (74), Expect = 0.086
Identities = 33/131 (25%), Positives = 53/131 (40%), Gaps = 16/131 (12%)
Frame = -3
Query: 281 TPIFLGKNAFSFFIVTDSLSLIASASSVLMFIGILTSRYAEEDFNTSLPAK--------- 331
T +F G + SF +++ +S+ +S+ + + + S A E +T + A
Sbjct: 395 TSVFTGSSTCSFSVLSTPVSIDSSSLNFDANVSLTGSLMAVEISSTFVSAFSSFLSSTAC 216
Query: 332 ---LLFGLFTIFLSVVFMMCSFCSALALMLKGY----RWIIITAIASSVIPILVFMFSLL 384
L F FT+ +SVV SFCS+ L+ W A S +
Sbjct: 215 VSFLTFSTFTLSVSVVAASASFCSSTFLVSTSTSLEDTWTASGASGSFLSSFAATAICS* 36
Query: 385 RLFSEVCISFL 395
L S C+SFL
Sbjct: 35 TLSSTACVSFL 3
>TC17801 weakly similar to GB|AAO42978.1|28416895|BT004732 At3g13950
{Arabidopsis thaliana;}, partial (22%)
Length = 423
Score = 30.8 bits (68), Expect = 0.43
Identities = 21/94 (22%), Positives = 41/94 (43%), Gaps = 22/94 (23%)
Frame = +2
Query: 241 EGRQWAKDIASSFTIVGTLIITIMFAAAFTVPGGNNQDKG-------------------T 281
+G +W K++ ++ ++I T+ F A PGG Q G
Sbjct: 122 DGEKWIKEMRGGLSMTASIIATMTFQLATNPPGGVFQANGGVPKTDARICWHNNTIQCPG 301
Query: 282 PIFLG---KNAFSFFIVTDSLSLIASASSVLMFI 312
LG ++ ++ F++ ++LS I+S S L+ +
Sbjct: 302 EAVLGVVYRHTYTNFLICNTLSFISSLSVCLLLV 403
>TC15938 similar to UP|O81340 (O81340) 26S proteasome regulatory subunit
S5A, partial (56%)
Length = 768
Score = 30.8 bits (68), Expect = 0.43
Identities = 18/71 (25%), Positives = 30/71 (41%), Gaps = 11/71 (15%)
Frame = -2
Query: 6 CRCCNKSCKVLSSNNDVVSSIWCTISTDQVTL*VYHEASS*LG-----------SLICVN 54
C CC+ SC +S + S WCT + ++ S G L+C++
Sbjct: 707 CCCCSSSCC*TTSTLSITSHNWCTYKYIR*SIWTRWNMSHAAGIIIINGCKECIQLLCLS 528
Query: 55 LIKFTMLSGIK 65
L+ FT + +K
Sbjct: 527 LVFFTKIDNVK 495
>TC15092 similar to UP|Q8QRX5 (Q8QRX5) UL145, partial (8%)
Length = 672
Score = 29.6 bits (65), Expect = 0.95
Identities = 20/56 (35%), Positives = 29/56 (51%)
Frame = -3
Query: 332 LLFGLFTIFLSVVFMMCSFCSALALMLKGYRWIIITAIASSVIPILVFMFSLLRLF 387
LLFG+ TIF+ + F++ SF S + Y II+ PI V +F L +F
Sbjct: 226 LLFGITTIFIFISFIIVSFISTRRRVFNFYTIHIIS------YPINVVIF*LTNIF 77
>TC8528 weakly similar to GB|AAO42978.1|28416895|BT004732 At3g13950
{Arabidopsis thaliana;}, partial (17%)
Length = 788
Score = 28.9 bits (63), Expect = 1.6
Identities = 19/91 (20%), Positives = 38/91 (40%), Gaps = 22/91 (24%)
Frame = +2
Query: 244 QWAKDIASSFTIVGTLIITIMFAAAFTVPGGNNQDKG----------------------T 281
+W K++ S ++ ++I T+ F A PGG Q G
Sbjct: 134 KWVKNMRGSVSLTASIIATMAFQLATNPPGGVFQANGGDSVAKIKSCLDNDAIQCPGEAV 313
Query: 282 PIFLGKNAFSFFIVTDSLSLIASASSVLMFI 312
+ ++ +S F+ +++S I+S S L+ +
Sbjct: 314 LAVVNEDDYSLFLTFNTISFISSLSVCLLLV 406
>AV421856
Length = 409
Score = 28.5 bits (62), Expect = 2.1
Identities = 16/49 (32%), Positives = 27/49 (54%), Gaps = 2/49 (4%)
Frame = +1
Query: 223 DGLTPHELFTKNHEHLL--NEGRQWAKDIASSFTIVGTLIITIMFAAAF 269
DG+T E TK H+HLL N + A D + S+ +++ ++ A +F
Sbjct: 58 DGVTTDESKTKKHQHLLFRNYLSRIAADSSGSYLPCVAVVLLLLLALSF 204
>BP072468
Length = 412
Score = 27.7 bits (60), Expect = 3.6
Identities = 27/107 (25%), Positives = 47/107 (43%), Gaps = 7/107 (6%)
Frame = -1
Query: 301 LIASASSVLMFIGILTSRYAEEDFNTSLPAKLLFGLFTIFLSVVFMMCSFCSALALMLKG 360
L+ S VL + +L +A+ + LP L +F + L + + F G
Sbjct: 322 LLLFQSGVLTLVSLL---FADA*ACSPLPLFFLLTIFLLLLFPISQLLIFNDTFDFSF*G 152
Query: 361 YRWI----IITAIASSVIPILVFMFSLLRL---FSEVCISFLRSYFL 400
I ++ + SS+ + F FSLLRL F+ +CI ++ F+
Sbjct: 151 RGMINIFRLLIGLVSSLSLVFYF*FSLLRLRFRFTFLCIGKWKAVFI 11
>BP064496
Length = 526
Score = 27.7 bits (60), Expect = 3.6
Identities = 16/52 (30%), Positives = 30/52 (56%)
Frame = -1
Query: 339 IFLSVVFMMCSFCSALALMLKGYRWIIITAIASSVIPILVFMFSLLRLFSEV 390
+ + VFM CS+ ++L G + +I A+ SSV+ ++F++ LFS +
Sbjct: 160 VLIYPVFMFCSY-----VLLLGPQCCLILALCSSVLFHVLFLYLFPVLFSSL 20
>AW720152
Length = 503
Score = 27.3 bits (59), Expect = 4.7
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = -1
Query: 361 YRWIIITAIASSVIPILVFMFSLLRLFSEVCISF 394
YRW + ++ ++ + F+ SLLR F C++F
Sbjct: 452 YRWRLSASLYQAISILW*FLSSLLRCFQRNCLNF 351
>BG662174
Length = 167
Score = 27.3 bits (59), Expect = 4.7
Identities = 14/51 (27%), Positives = 28/51 (54%)
Frame = -3
Query: 317 SRYAEEDFNTSLPAKLLFGLFTIFLSVVFMMCSFCSALALMLKGYRWIIIT 367
SR + +++TS K FG + SVV ++ S +A+ ++ + W++ T
Sbjct: 165 SRDSSREYSTSADRKCFFGAGLVAASVVVVVGSVVAAVVMVDEHGPWLVWT 13
>TC18970 weakly similar to UP|Q8I8F0 (Q8I8F0) Chromosome scaffold protein
p85, partial (4%)
Length = 562
Score = 26.9 bits (58), Expect = 6.2
Identities = 18/62 (29%), Positives = 30/62 (48%)
Frame = +1
Query: 326 TSLPAKLLFGLFTIFLSVVFMMCSFCSALALMLKGYRWIIITAIASSVIPILVFMFSLLR 385
T++ K + GL + L V+ ++CSFC A+ L + +T S + P + F L
Sbjct: 319 TNILIKHMHGL--V*LVVLLLLCSFCIAMPLQVNAQNGKSMTITMSCLFPSSIRTF*FLS 492
Query: 386 LF 387
F
Sbjct: 493 *F 498
>TC9315
Length = 632
Score = 26.9 bits (58), Expect = 6.2
Identities = 15/29 (51%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Frame = +1
Query: 373 VIPILVFMFSLLRL-FSEVCISFLRSYFL 400
++ L F+ SLLR+ FS VCISF R+ L
Sbjct: 448 IVSRLYFLGSLLRVRFSSVCISFHRNALL 534
>TC19530
Length = 602
Score = 26.9 bits (58), Expect = 6.2
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 235 HEHLLNEGRQWAKDIASSFTIVGTL 259
H H+LN G+ W K I+ + ++ TL
Sbjct: 373 HFHILNTGKFWVKCISEKYCLLFTL 299
>BP075633
Length = 481
Score = 26.9 bits (58), Expect = 6.2
Identities = 17/40 (42%), Positives = 24/40 (59%), Gaps = 2/40 (5%)
Frame = -3
Query: 295 VTDSL--SLIASASSVLMFIGILTSRYAEEDFNTSLPAKL 332
VTD++ S + ++ +L FIG LTS Y DF T+ KL
Sbjct: 131 VTDNVHHSSVFPSNDLLTFIGDLTSTYYVFDFATTKKKKL 12
>AV410313
Length = 367
Score = 26.6 bits (57), Expect = 8.0
Identities = 12/22 (54%), Positives = 17/22 (76%)
Frame = +3
Query: 87 KIEKISSESELHQCSIHDAMLQ 108
K K SSE++LHQ S+ D++LQ
Sbjct: 132 KSAKSSSEAKLHQTSVLDSILQ 197
>BP046365
Length = 516
Score = 26.6 bits (57), Expect = 8.0
Identities = 14/59 (23%), Positives = 28/59 (46%)
Frame = -3
Query: 331 KLLFGLFTIFLSVVFMMCSFCSALALMLKGYRWIIITAIASSVIPILVFMFSLLRLFSE 389
++LF F +FLS ++C Y+++ S+ P L F+ S+++ F +
Sbjct: 253 RILFSWFLLFLSYYLLLCR-------KEYNYKFLSFVCT*STCRPFLCFIVSMIKSFKQ 98
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.139 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,378,792
Number of Sequences: 28460
Number of extensions: 101154
Number of successful extensions: 763
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 756
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 759
length of query: 407
length of database: 4,897,600
effective HSP length: 92
effective length of query: 315
effective length of database: 2,279,280
effective search space: 717973200
effective search space used: 717973200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146721.2