BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146719.4 - phase: 0 /pseudo
(471 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 67 8e-12
AU251673 59 1e-09
BI418821 55 2e-08
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 52 3e-07
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 49 2e-06
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 49 2e-06
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 38 0.004
BE122516 33 0.13
TC9306 similar to UP|Q42922 (Q42922) Annexin (Fragment), partial... 32 0.22
TC17929 31 0.50
TC13690 similar to UP|Q9SGS0 (Q9SGS0) T23E18.6, partial (31%) 29 1.4
TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial ... 29 1.4
TC18767 29 1.9
TC9039 28 3.2
BP079571 23 3.7
TC9283 28 4.2
AV420911 27 5.5
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 27 5.5
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 27 5.5
BP039068 27 7.2
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 66.6 bits (161), Expect = 8e-12
Identities = 43/147 (29%), Positives = 68/147 (46%), Gaps = 7/147 (4%)
Frame = -2
Query: 224 KPYSAPTDKGKQRL------NDERRPKRRDAPTEIICFKCGEKGHKSNVCDRDEKKCFRC 277
KP+ P ++G N RP + D +EI+C +C +KGH +N C + C+ C
Sbjct: 434 KPFQRPQNRGTSSGYSHSFGNFVPRPTQSDT-SEIVCHRCSKKGHFANRC--PDLVCWNC 264
Query: 278 GQKGHTLADCKRGDV-VCYNCNEEGHISSQCTQPKKVRTGGKVFALTGTQTVNEDRLIRG 336
+ GH+ DC V N + + K+ +V+ ++G ++ D LIR
Sbjct: 263 QKTGHSGKDCTNPKVEAATNAIAARRPAPAANKGKRPVASARVYTVSGAESHRADGLIRS 84
Query: 337 TCFFNSTHLIAIIDTGATHCFIVVDCA 363
N L + D+GATH FI + CA
Sbjct: 83 VGSVNCKPLTILFDSGATHSFIDLACA 3
>AU251673
Length = 413
Score = 59.3 bits (142), Expect = 1e-09
Identities = 39/139 (28%), Positives = 67/139 (48%), Gaps = 2/139 (1%)
Frame = +2
Query: 69 CSEVQKVRFGTHMLAEEADDWWVSLLPILEQDGAAVTWVVFRREFLNMYFPEDVRG--KK 126
CS+ + V + L A DW+ L TW F EF+N + P+ VR +
Sbjct: 5 CSDTRAVELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVR 184
Query: 127 EIEFLEMKQGDMSVTEYAAKFVELAKFYPHYTTETAEFSKCIKFENGLRADIKRAIGYEK 186
+ E LE +G M+V+EY+A F L+++ P+ E + +F GL+ + +++ K
Sbjct: 185 DFERLEQAEG-MTVSEYSAHFTHLSRYVPY---PLLEEERVKRFVRGLKEYLFKSVVGSK 352
Query: 187 IRIFSELVSSCRIYEEDTK 205
SE++S + E+ K
Sbjct: 353 SSTLSEVLSLALLVEQRQK 409
>BI418821
Length = 614
Score = 55.1 bits (131), Expect = 2e-08
Identities = 23/71 (32%), Positives = 31/71 (43%), Gaps = 15/71 (21%)
Frame = +2
Query: 254 CFKCGEKGHKSNVCDRDEKK--------CFRCGQKGHTLADCKR-------GDVVCYNCN 298
C+ CG+ GH + C R C+ CG GH DC R G CYNC
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGGAGCYNCG 571
Query: 299 EEGHISSQCTQ 309
+ GH++ C +
Sbjct: 572 DTGHLARDCNR 604
Score = 45.1 bits (105), Expect = 3e-05
Identities = 18/52 (34%), Positives = 23/52 (43%), Gaps = 8/52 (15%)
Frame = +2
Query: 274 CFRCGQKGHTLADCKR--------GDVVCYNCNEEGHISSQCTQPKKVRTGG 317
C+ CG GH DC R G CYNC + GH++ C + GG
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGG 547
Score = 40.4 bits (93), Expect = 6e-04
Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 7/45 (15%)
Frame = +2
Query: 254 CFKCGEKGHKSNVCDRDEKK-------CFRCGQKGHTLADCKRGD 291
C+ CG+ GH + C+R C+ CG GH DC R +
Sbjct: 476 CYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDCNRSN 610
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 51.6 bits (122), Expect = 3e-07
Identities = 28/96 (29%), Positives = 42/96 (43%)
Frame = +2
Query: 212 SERRGKGQ*SRPKPYSAPTDKGKQRLNDERRPKRRDAPTEIICFKCGEKGHKSNVCDRDE 271
S R + + P +D+ R RR RR + +C C GH + C +
Sbjct: 218 SRSRSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECP-NV 394
Query: 272 KKCFRCGQKGHTLADCKRGDVVCYNCNEEGHISSQC 307
C CG GH ++C + C+NC E GH++S C
Sbjct: 395 AICHNCGLPGHIASECTTKSL-CWNCKEPGHMASSC 499
Score = 36.2 bits (82), Expect = 0.012
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +2
Query: 274 CFRCGQKGHTLADCKRGDVVCYNCNEEGHISSQCT 308
C C + GH +C +C+NC GHI+S+CT
Sbjct: 344 CKNCKRPGHFARECPNV-AICHNCGLPGHIASECT 445
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 48.9 bits (115), Expect = 2e-06
Identities = 24/57 (42%), Positives = 31/57 (54%), Gaps = 5/57 (8%)
Frame = +2
Query: 273 KCFRCGQKGHTLADCKRGD--VVCYNCNEEGHISSQC-TQPKK--VRTGGKVFALTG 324
+CF CG GH DCK GD CY C + GH+ C PKK +T ++F+ TG
Sbjct: 368 RCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKNEWQTWKELFSFTG 538
Score = 40.8 bits (94), Expect = 5e-04
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +2
Query: 254 CFKCGEKGHKSNVCDRDE--KKCFRCGQKGHTLADCK 288
CF CG GH + C + KC+RCG +GH +CK
Sbjct: 371 CFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCK 481
Score = 27.3 bits (59), Expect = 5.5
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +2
Query: 254 CFKCGEKGHKSNVCDRDEKK 273
C++CG++GH C KK
Sbjct: 437 CYRCGDRGHVERNCKNSPKK 496
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 48.5 bits (114), Expect = 2e-06
Identities = 22/44 (50%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
Frame = +1
Query: 273 KCFRCGQKGHTLADCKRGD--VVCYNCNEEGHISSQC-TQPKKV 313
+CF CG GH DCK GD CY C E GHI C PKK+
Sbjct: 388 RCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKKL 519
Score = 42.0 bits (97), Expect = 2e-04
Identities = 21/58 (36%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Frame = +1
Query: 233 GKQRLNDERRPKRRDAPTEIICFKCGEKGHKSNVCDRDE--KKCFRCGQKGHTLADCK 288
G R D R P CF CG GH + C + KC+RCG++GH +CK
Sbjct: 328 GGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCK 501
Score = 28.9 bits (63), Expect = 1.9
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +1
Query: 254 CFKCGEKGHKSNVCDRDEKKCFR 276
C++CGE+GH C KK R
Sbjct: 457 CYRCGERGHIEKNCKNSPKKLSR 525
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 37.7 bits (86), Expect = 0.004
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +3
Query: 274 CFRCGQKGHTLADCKRGDVVCYNCNEEGHISSQC 307
C C Q GH DC ++C+NC GH++ +C
Sbjct: 9 CRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
Score = 34.3 bits (77), Expect = 0.045
Identities = 13/39 (33%), Positives = 18/39 (45%)
Frame = +3
Query: 252 IICFKCGEKGHKSNVCDRDEKKCFRCGQKGHTLADCKRG 290
++C C + GH S C C CG +GH +C G
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECPSG 119
Score = 27.7 bits (60), Expect = 4.2
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = +3
Query: 292 VVCYNCNEEGHISSQCTQP 310
VVC NC + GH+S C P
Sbjct: 3 VVCRNCQQLGHMSRDCMGP 59
>BE122516
Length = 364
Score = 32.7 bits (73), Expect = 0.13
Identities = 17/42 (40%), Positives = 23/42 (54%)
Frame = +2
Query: 422 GMNWLEYNHFHINCFSKTVHFSSAEEESEAKFLTTKQLKQLE 463
GMNWL N +NC KTV F ++E +++ T K K E
Sbjct: 17 GMNWLTANDATLNCRKKTVTFGTSEGDAKRVKRTDKVGKASE 142
>TC9306 similar to UP|Q42922 (Q42922) Annexin (Fragment), partial (59%)
Length = 662
Score = 32.0 bits (71), Expect = 0.22
Identities = 22/65 (33%), Positives = 32/65 (48%), Gaps = 6/65 (9%)
Frame = -3
Query: 358 IVVDCAYKLGLIVSDMNGEMVVETPAKGSVTTSLVCLSCLLSM----FGRDFEVDLVCL- 412
I+ +C +K ++D+ M+ G + TSLVCL CL + G +F D V
Sbjct: 570 IITECPHKRNQKLADVPRSMIGNVFFNGPLETSLVCLLCLKEVK*RSSGSNFH*DPVVFS 391
Query: 413 -PLSG 416
PLSG
Sbjct: 390 QPLSG 376
>TC17929
Length = 791
Score = 30.8 bits (68), Expect = 0.50
Identities = 10/37 (27%), Positives = 20/37 (54%)
Frame = +2
Query: 234 KQRLNDERRPKRRDAPTEIICFKCGEKGHKSNVCDRD 270
+ ++ +E + D+ C++CGE GHK C ++
Sbjct: 2 RAQIGEETSNRPNDSKFRQTCYRCGESGHKMRNCPKE 112
Score = 27.3 bits (59), Expect = 5.5
Identities = 7/16 (43%), Positives = 12/16 (74%)
Frame = +2
Query: 274 CFRCGQKGHTLADCKR 289
C+RCG+ GH + +C +
Sbjct: 62 CYRCGESGHKMRNCPK 109
Score = 26.6 bits (57), Expect = 9.4
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +2
Query: 294 CYNCNEEGHISSQCTQPKKVRTGGKV 319
CY C E GH C PK+ +G K+
Sbjct: 62 CYRCGESGHKMRNC--PKEHSSGEKI 133
>TC13690 similar to UP|Q9SGS0 (Q9SGS0) T23E18.6, partial (31%)
Length = 621
Score = 29.3 bits (64), Expect = 1.4
Identities = 19/55 (34%), Positives = 32/55 (57%), Gaps = 1/55 (1%)
Frame = -2
Query: 363 AYKLGLIVSDMNGEMVVETPAKGSVTTSLVCLSCLLSMFGR-DFEVDLVCLPLSG 416
A++L L S +N + P++ +VT ++CLSCL++ G F + L L L+G
Sbjct: 224 AHQLYL*DSQLNA*KSISFPSRKTVT-EIICLSCLITWGGSLSFLISLALLFLAG 63
>TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial (90%)
Length = 1737
Score = 29.3 bits (64), Expect = 1.4
Identities = 22/78 (28%), Positives = 30/78 (38%), Gaps = 10/78 (12%)
Frame = +1
Query: 244 KRRDAPTEIICFKCGEKGHKSNVCDRDEKKCFRCGQKGHT-------LADCKRGDVVCYN 296
K+ ++C KC KG KS R CF C G L ++ VC +
Sbjct: 559 KKLSLSRNVLCLKCKGKGSKSGTAGR----CFGCQGTGMKVIRRQIGLGMVQQMQHVCSD 726
Query: 297 CNEEGHISSQ---CTQPK 311
C G + S+ C Q K
Sbjct: 727 CRGTGEVISERDRCPQCK 780
>TC18767
Length = 1004
Score = 28.9 bits (63), Expect = 1.9
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +2
Query: 270 DEKKCFRCGQKGHTLADCKR 289
D +CF CG H+L +C R
Sbjct: 155 DASRCFNCGSYNHSLRECSR 214
>TC9039
Length = 1218
Score = 28.1 bits (61), Expect = 3.2
Identities = 9/22 (40%), Positives = 14/22 (62%)
Frame = +2
Query: 288 KRGDVVCYNCNEEGHISSQCTQ 309
++ D CY CN GH+ +CT+
Sbjct: 827 QKEDDTCYFCNVSGHMKKKCTK 892
Score = 28.1 bits (61), Expect = 3.2
Identities = 22/85 (25%), Positives = 32/85 (36%), Gaps = 6/85 (7%)
Frame = +2
Query: 191 SELVSSCRIYEEDTKAHYKVMSERRGKGQ*SRPKPYSAPTDKGKQRLNDERRPKRRDAPT 250
+EL+S C EE K K + S DKGK++ E + + DAP
Sbjct: 671 NELISFCVQEEERLKQERKESAHF-----------VSTSKDKGKRKKTVEPKNEAADAPA 817
Query: 251 ------EIICFKCGEKGHKSNVCDR 269
+ C+ C GH C +
Sbjct: 818 PKKQKEDDTCYFCNVSGHMKKKCTK 892
>BP079571
Length = 414
Score = 23.5 bits (49), Expect(2) = 3.7
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = -1
Query: 254 CFKCGEKGHKSNVCD 268
CF+ GE GH + CD
Sbjct: 357 CFRFGEVGHLARDCD 313
Score = 22.7 bits (47), Expect(2) = 3.7
Identities = 7/12 (58%), Positives = 8/12 (66%)
Frame = -3
Query: 271 EKKCFRCGQKGH 282
E CF CG+ GH
Sbjct: 271 ETHCFHCGKPGH 236
>TC9283
Length = 1307
Score = 27.7 bits (60), Expect = 4.2
Identities = 11/42 (26%), Positives = 25/42 (59%)
Frame = +2
Query: 60 VERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPILEQDG 101
VER+ R M +E ++ +G H L+ ++ +++ L+++G
Sbjct: 905 VERLCRAMGGAEKVEIEYGNHSLSNRVEEAVQAIIDFLKREG 1030
>AV420911
Length = 418
Score = 27.3 bits (59), Expect = 5.5
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +2
Query: 291 DVVCYNCNEEGHISSQC 307
D+ CY C E GH + +C
Sbjct: 365 DLKCYECGEPGHFAREC 415
Score = 26.9 bits (58), Expect = 7.2
Identities = 7/19 (36%), Positives = 13/19 (67%)
Frame = +2
Query: 270 DEKKCFRCGQKGHTLADCK 288
++ KC+ CG+ GH +C+
Sbjct: 362 EDLKCYECGEPGHFARECR 418
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 27.3 bits (59), Expect = 5.5
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +1
Query: 291 DVVCYNCNEEGHISSQC 307
D+ CY C E GH + +C
Sbjct: 157 DLKCYECGEPGHFAREC 207
Score = 26.9 bits (58), Expect = 7.2
Identities = 7/19 (36%), Positives = 13/19 (67%)
Frame = +1
Query: 270 DEKKCFRCGQKGHTLADCK 288
++ KC+ CG+ GH +C+
Sbjct: 154 EDLKCYECGEPGHFARECR 210
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 27.3 bits (59), Expect = 5.5
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +1
Query: 291 DVVCYNCNEEGHISSQC 307
D+ CY C E GH + +C
Sbjct: 370 DMKCYECGEPGHFAREC 420
Score = 26.6 bits (57), Expect = 9.4
Identities = 7/18 (38%), Positives = 12/18 (65%)
Frame = +1
Query: 271 EKKCFRCGQKGHTLADCK 288
+ KC+ CG+ GH +C+
Sbjct: 370 DMKCYECGEPGHFARECR 423
>BP039068
Length = 467
Score = 26.9 bits (58), Expect = 7.2
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +3
Query: 293 VCYNCNEEGHISSQC 307
VC NC E GH S+ C
Sbjct: 309 VCMNCQETGHASNDC 353
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,993,880
Number of Sequences: 28460
Number of extensions: 137895
Number of successful extensions: 649
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 579
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 626
length of query: 471
length of database: 4,897,600
effective HSP length: 94
effective length of query: 377
effective length of database: 2,222,360
effective search space: 837829720
effective search space used: 837829720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146719.4