BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146719.10 + phase: 0 /pseudo
(112 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP030832 99 3e-26
TC13702 homologue to PIR|A84591|A84591 26S proteasome regulatory... 77 5e-16
TC18295 similar to UP|Q39224 (Q39224) SRG1 mRNA (F6I1.30/F6I1.30... 29 0.13
BP034914 25 1.9
TC14596 homologue to UP|Q9FGU1 (Q9FGU1) Gb|AAD34459.1 (SPP30-lik... 25 1.9
TC8121 UP|Q9LKJ5 (Q9LKJ5) Multifunctional transport intrinsic me... 24 3.2
TC12103 23 5.4
TC13821 similar to UP|TPCS_ANGAN (P81660) Troponin C, skeletal m... 23 7.1
AV406716 23 7.1
TC18825 similar to UP|BAD08451 (BAD08451) Glucose-6-phosphate is... 23 7.1
BP069165 23 7.1
BP075559 23 7.1
TC19968 similar to UP|Q9AXM0 (Q9AXM0) Prohibitin 1-like protein,... 23 9.3
TC19432 23 9.3
TC15983 homologue to UP|Q9MUE2 (Q9MUE2) S-adenosyl-L-methionine ... 23 9.3
>BP030832
Length = 507
Score = 98.6 bits (244), Expect(2) = 3e-26
Identities = 50/55 (90%), Positives = 51/55 (91%)
Frame = -1
Query: 41 TVGENLKPLLVPLRVGQAVDVVGQAGRPKKITGFQTHSTSVLLAAGDRAELATEK 95
TV ENLKPL VP+RVGQAVDVVGQAGRPK ITGFQTHST VLLAAGDRAELATEK
Sbjct: 507 TVDENLKPLSVPVRVGQAVDVVGQAGRPKTITGFQTHSTPVLLAAGDRAELATEK 343
Score = 32.7 bits (73), Expect(2) = 3e-26
Identities = 13/17 (76%), Positives = 16/17 (93%)
Frame = -3
Query: 95 KYIPISPVLEAFVILKE 111
+YIP+SP+LE FVILKE
Sbjct: 268 RYIPLSPILEGFVILKE 218
>TC13702 homologue to PIR|A84591|A84591 26S proteasome regulatory subunit
S2 [imported] - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (9%)
Length = 516
Score = 76.6 bits (187), Expect = 5e-16
Identities = 38/43 (88%), Positives = 39/43 (90%)
Frame = +2
Query: 39 LLTVGENLKPLLVPLRVGQAVDVVGQAGRPKKITGFQTHSTSV 81
LLTV ENLKPL VP+RVGQAVDVVGQAGRPK ITGFQTHST V
Sbjct: 2 LLTVDENLKPLSVPVRVGQAVDVVGQAGRPKTITGFQTHSTPV 130
Score = 58.5 bits (140), Expect = 2e-10
Identities = 28/43 (65%), Positives = 34/43 (78%)
Frame = +3
Query: 69 KKITGFQTHSTSVLLAAGDRAELATEKYIPISPVLEAFVILKE 111
+++ F+ LLAAGDRAELATEKYIP+SP+LE FVILKE
Sbjct: 93 RQLLAFRPTQPLFLLAAGDRAELATEKYIPLSPILEGFVILKE 221
>TC18295 similar to UP|Q39224 (Q39224) SRG1 mRNA (F6I1.30/F6I1.30)
(At1g17020/F6I1.30), partial (29%)
Length = 561
Score = 28.9 bits (63), Expect = 0.13
Identities = 22/77 (28%), Positives = 32/77 (40%), Gaps = 7/77 (9%)
Frame = +3
Query: 39 LLTVGENLKPLLVPLRVGQAVDVVGQAGRPKKITGFQTHSTS----VLLAAGDRAELATE 94
L T L L G ++ +P+K+ G HS S +LL + L
Sbjct: 324 LKTETNELVELFEDTSQGMRMNYYPPCPQPEKVIGLNPHSDSGAFTILLQVNEMEGLQIR 503
Query: 95 K---YIPISPVLEAFVI 108
K ++PI P+ AFVI
Sbjct: 504 KDGIWVPIKPLSNAFVI 554
>BP034914
Length = 544
Score = 25.0 bits (53), Expect = 1.9
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3
Query: 42 VGENLKPLLVPLRVGQAVDVVGQAGR 67
VG +LK LL + GQAVD+ G + R
Sbjct: 360 VGNDLKQLLQMIDDGQAVDIKGISER 437
>TC14596 homologue to UP|Q9FGU1 (Q9FGU1) Gb|AAD34459.1 (SPP30-like protein)
(At5g58030), partial (38%)
Length = 449
Score = 25.0 bits (53), Expect = 1.9
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = -2
Query: 38 MLLTVGENL-KPLLVPLRVGQAV-DVVGQAGRPKKITGFQTHSTSVLLAA 85
+LL +GE L K + V L + A ++ GR ++ GF+ H SVLL +
Sbjct: 298 LLLALGEGLVKDVTVLLHLSDADHSLLLLGGRRRRTEGFREH*NSVLLCS 149
>TC8121 UP|Q9LKJ5 (Q9LKJ5) Multifunctional transport intrinsic membrane
protein 2, complete
Length = 1213
Score = 24.3 bits (51), Expect = 3.2
Identities = 10/37 (27%), Positives = 21/37 (56%)
Frame = +2
Query: 14 YVLYFLVLAMQLTLGYYILLQKPTMLLTVGENLKPLL 50
+V+ F++L M YYI+ M + + + ++P+L
Sbjct: 1079 FVILFVILHMLFPFSYYIVSLYKVMKVILDKCIEPIL 1189
>TC12103
Length = 627
Score = 23.5 bits (49), Expect = 5.4
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 38 MLLTVGENLKPLLVPLRVGQ 57
+LLTVG+ L+ +V L VGQ
Sbjct: 184 LLLTVGDRLEVTIVLLLVGQ 243
>TC13821 similar to UP|TPCS_ANGAN (P81660) Troponin C, skeletal muscle
(TNC), partial (8%)
Length = 515
Score = 23.1 bits (48), Expect = 7.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 15 VLYFLVLAMQLTLGYYILLQKPTML 39
V +FLVL LT +LLQK +L
Sbjct: 131 VSFFLVLFTSLTCSLSLLLQKIVLL 205
>AV406716
Length = 428
Score = 23.1 bits (48), Expect = 7.1
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +1
Query: 62 VGQAGRPKKITGFQTHSTSVLLAA 85
+ + G+PK+I+ THS S+ +A
Sbjct: 136 IAREGKPKRISQDPTHSHSITSSA 207
>TC18825 similar to UP|BAD08451 (BAD08451) Glucose-6-phosphate isomerase,
partial (39%)
Length = 773
Score = 23.1 bits (48), Expect = 7.1
Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 6/34 (17%)
Frame = -3
Query: 13 HYVLY-----FLVLAMQLTLGYYILL-QKPTMLL 40
H++ Y FLVL+ Q LG ILL ++P++LL
Sbjct: 246 HFLQYCPTKNFLVLSQQPLLGNSILLHERPSVLL 145
>BP069165
Length = 387
Score = 23.1 bits (48), Expect = 7.1
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = +2
Query: 12 YHYVLYFLVLAMQLTLGYYILLQKPTMLL 40
Y +VLY + M ++ ++L PT+L+
Sbjct: 86 YIFVLYIYIPHMTTSMDLFLLTSLPTILI 172
>BP075559
Length = 666
Score = 23.1 bits (48), Expect = 7.1
Identities = 10/32 (31%), Positives = 15/32 (46%)
Frame = +3
Query: 12 YHYVLYFLVLAMQLTLGYYILLQKPTMLLTVG 43
YHY L + +++ YY KPT + G
Sbjct: 144 YHYSLNHRSQILHISMVYYSKFNKPTFTVANG 239
>TC19968 similar to UP|Q9AXM0 (Q9AXM0) Prohibitin 1-like protein, partial
(57%)
Length = 564
Score = 22.7 bits (47), Expect = 9.3
Identities = 16/57 (28%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = +3
Query: 34 QKPTMLLTVGENLKPLLVPLRVGQAVD-VVGQAGRPKKITGFQTHSTSVLLAAGDRA 89
Q PT+ T+GEN ++P + + + VV Q + IT + S + +RA
Sbjct: 363 QLPTVYRTLGENYNERVLPSIIHETLKAVVAQYNASQLITQRENVSREIRKILTERA 533
>TC19432
Length = 461
Score = 22.7 bits (47), Expect = 9.3
Identities = 8/16 (50%), Positives = 14/16 (87%)
Frame = +2
Query: 47 KPLLVPLRVGQAVDVV 62
KPL++ ++VGQ+ DV+
Sbjct: 383 KPLILGIQVGQSTDVM 430
>TC15983 homologue to UP|Q9MUE2 (Q9MUE2) S-adenosyl-L-methionine
Mg-protoporphyrin IX methyltranserase , partial (17%)
Length = 559
Score = 22.7 bits (47), Expect = 9.3
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +2
Query: 12 YHYVLYFLVLAMQLTLGYYIL 32
YHYV +FLV M L YY L
Sbjct: 464 YHYVYFFLV*KM---LMYYFL 517
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.333 0.149 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,618,387
Number of Sequences: 28460
Number of extensions: 17243
Number of successful extensions: 102
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of query: 112
length of database: 4,897,600
effective HSP length: 88
effective length of query: 24
effective length of database: 2,393,120
effective search space: 57434880
effective search space used: 57434880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 47 (22.7 bits)
Medicago: description of AC146719.10