BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146711.13 - phase: 0 /pseudo
(895 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP040614 148 3e-36
BP063922 86 2e-20
TC15461 similar to UP|O24147 (O24147) Kinesin-like protein, part... 92 4e-19
AV773555 86 3e-17
TC12031 homologue to UP|ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-l... 71 9e-13
BP062438 69 3e-12
TC15187 similar to UP|O24147 (O24147) Kinesin-like protein, part... 67 1e-11
CB829109 66 2e-11
AV407795 62 5e-10
AV775627 53 2e-07
AV421105 47 1e-05
CB827128 40 0.001
BP044474 37 0.011
BP067112 36 0.031
AV778230 36 0.031
BP037078 34 0.090
BP049886 30 1.3
AI967308 30 1.7
BI417263 30 2.2
TC13753 similar to UP|Q9LQQ6 (Q9LQQ6) F24B9.6, partial (38%) 28 5.0
>BP040614
Length = 504
Score = 148 bits (374), Expect = 3e-36
Identities = 78/165 (47%), Positives = 115/165 (69%), Gaps = 1/165 (0%)
Frame = +1
Query: 501 NYRTLEHLFRVSKERSETFSYDISVSVLEVYNEQIRDLLATGPASKRLEIKQNYEGHH-H 559
NY L LF +SK+R+++ Y++ V ++E+YNEQ+RDLL + +++RL+I+ N + + +
Sbjct: 10 NY*GLRDLFHISKDRADSIKYEVFVQMIEIYNEQVRDLLVSNGSNRRLDIRNNSQLNGLN 189
Query: 560 VPGVVEAKVDNISDVWTVLQAGSNARAVGSNNVNEHSSRSHCMLCIMVKTKNLMNGECTK 619
VP V DV +++ G RAVG+ +NE SSRSH +L + V+ + L++G +
Sbjct: 190 VPDAYLVPVTCTQDVLYLMEVGQKNRAVGATALNERSSRSHSVLTVHVRGRELVSGSILR 369
Query: 620 SKLWLVDLSGSERLAKTDVQGERLKEAQNINRSLSALGDVISALA 664
L LVDL+GSER+ K++ GERLKEAQ+INRSLSALGDVISALA
Sbjct: 370 GCLHLVDLAGSERVEKSEAVGERLKEAQHINRSLSALGDVISALA 504
>BP063922
Length = 537
Score = 86.3 bits (212), Expect(2) = 2e-20
Identities = 52/122 (42%), Positives = 73/122 (59%)
Frame = +2
Query: 563 VVEAKVDNISDVWTVLQAGSNARAVGSNNVNEHSSRSHCMLCIMVKTKNLMNGECTKSKL 622
+ + KVDN D TVL+A +R +N SH ++ I + NL+ GE + SKL
Sbjct: 182 LAQEKVDNPLDFSTVLKAAFQSRGNDLLKINV----SHLIVTIHIFYNNLITGENSYSKL 349
Query: 623 WLVDLSGSERLAKTDVQGERLKEAQNINRSLSALGDVISALAAKSSHIPYRNSKLTHLLQ 682
LVDL+GSE D GER+ + ++ +SLSALGDV+S+L +K +PY NS LT +L
Sbjct: 350 SLVDLAGSEGSITEDDSGERVTDLLHVMKSLSALGDVLSSLTSKKDIVPYENSALTKVLA 529
Query: 683 DS 684
DS
Sbjct: 530 DS 535
Score = 30.4 bits (67), Expect(2) = 2e-20
Identities = 13/32 (40%), Positives = 20/32 (61%)
Frame = +1
Query: 508 LFRVSKERSETFSYDISVSVLEVYNEQIRDLL 539
++ + + + T Y V+V E+YNEQ RDLL
Sbjct: 25 IYLANLDTTSTSQYKFCVTVCELYNEQTRDLL 120
>TC15461 similar to UP|O24147 (O24147) Kinesin-like protein, partial (9%)
Length = 527
Score = 92.0 bits (227), Expect = 4e-19
Identities = 53/122 (43%), Positives = 76/122 (61%), Gaps = 2/122 (1%)
Frame = +3
Query: 657 GDVISALAAKSSHIPYRNSKLTHLLQDSLGGDSKTLMFVQISPSDQDVGETLSSLNFATR 716
GDVISAL++ HIPYRN KLT L+ DSLGG++KTLMFV +SP + + ET +SL +A+R
Sbjct: 3 GDVISALSSGGQHIPYRNHKLTMLMSDSLGGNAKTLMFVNVSPIESSLDETHNSLMYASR 182
Query: 717 VRGVELDPVKKQIDTGELQKTKAMLD--KARSECRCKEESLRKLEESLQNIESKAKGKDN 774
VR + DP K + + E+ + K + K ++ R +EE L E +Q + + DN
Sbjct: 183 VRSIVNDP-SKNVSSKEIMRLKKQVAYWKEQAGRRGEEEDL----EEIQEVRPTKEKTDN 347
Query: 775 IH 776
H
Sbjct: 348 RH 353
>AV773555
Length = 460
Score = 85.5 bits (210), Expect = 3e-17
Identities = 43/66 (65%), Positives = 52/66 (78%)
Frame = -1
Query: 652 SLSALGDVISALAAKSSHIPYRNSKLTHLLQDSLGGDSKTLMFVQISPSDQDVGETLSSL 711
SLS+L DVI ALA + HIP+R+SKLT+LLQ +LGGDSKT+MFV ISP GE+L SL
Sbjct: 460 SLSSLSDVIFALAQQDDHIPFRHSKLTYLLQPALGGDSKTVMFVNISPDPASAGESLCSL 281
Query: 712 NFATRV 717
FA+RV
Sbjct: 280 RFASRV 263
>TC12031 homologue to UP|ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-like protein
A), partial (7%)
Length = 740
Score = 70.9 bits (172), Expect = 9e-13
Identities = 36/59 (61%), Positives = 42/59 (71%)
Frame = +2
Query: 673 RNSKLTHLLQDSLGGDSKTLMFVQISPSDQDVGETLSSLNFATRVRGVELDPVKKQIDT 731
RNSKLT+LLQ LGGDSKTLMFV ISP VGE+L SL FA+RV E+ ++Q T
Sbjct: 2 RNSKLTYLLQPCLGGDSKTLMFVNISPDQSSVGESLCSLRFASRVNACEIGIPRRQTQT 178
>BP062438
Length = 498
Score = 69.3 bits (168), Expect = 3e-12
Identities = 37/94 (39%), Positives = 61/94 (64%)
Frame = +3
Query: 472 GYNVCIFAYGQTGTGKTFTMEGTEQNRGVNYRTLEHLFRVSKERSETFSYDISVSVLEVY 531
G+N +FAYGQT +GKT TM G++ + GV R + LF++ ++ + + + +S +E+Y
Sbjct: 222 GFNGTVFAYGQTNSGKTHTMRGSKADPGVIPRAVRDLFQIIQQDVDR-EFLLRMSYMEIY 398
Query: 532 NEQIRDLLATGPASKRLEIKQNYEGHHHVPGVVE 565
NE+I DLLA P ++L+I +N E +V G+ E
Sbjct: 399 NEEINDLLA--PEHRKLQIHENLERGIYVAGLRE 494
>TC15187 similar to UP|O24147 (O24147) Kinesin-like protein, partial (8%)
Length = 611
Score = 67.0 bits (162), Expect = 1e-11
Identities = 33/72 (45%), Positives = 51/72 (70%)
Frame = +2
Query: 670 IPYRNSKLTHLLQDSLGGDSKTLMFVQISPSDQDVGETLSSLNFATRVRGVELDPVKKQI 729
IPYRN KLT L+ DSLGG+++TLMFV +SP + + ET +SL +A+RVR + DP + +
Sbjct: 2 IPYRNHKLTMLMSDSLGGNAQTLMFVNVSPVESSLDETHNSLMYASRVRSIINDP-SQNV 178
Query: 730 DTGELQKTKAML 741
+ E+ + K ++
Sbjct: 179 ASKEIARLKKLV 214
>CB829109
Length = 514
Score = 66.2 bits (160), Expect = 2e-11
Identities = 44/150 (29%), Positives = 80/150 (53%), Gaps = 1/150 (0%)
Frame = +2
Query: 460 ADASSMVISVLDGYNVCIFAYGQTGTGKTFTMEGTEQNRGVNYRTLEHLFR-VSKERSET 518
A ++ ++ + FAYGQTG+GKT+TM+ + R E L R + + +
Sbjct: 5 ATVEPIIPTIFERTKATCFAYGQTGSGKTYTMQ------PLPLRAAEDLVRQLHRPVYQN 166
Query: 519 FSYDISVSVLEVYNEQIRDLLATGPASKRLEIKQNYEGHHHVPGVVEAKVDNISDVWTVL 578
+ + +S E+Y ++ DLL+ K+L ++++ + G+ E +V ++ V +
Sbjct: 167 QKFKLWLSYFEIYGGKLFDLLSD---RKKLCMREDGRQQVCIVGLQEFEVFDVQIVKEFI 337
Query: 579 QAGSNARAVGSNNVNEHSSRSHCMLCIMVK 608
+ G+ AR+ GS NE SSRSH +L ++VK
Sbjct: 338 EKGNAARSTGSTGANEESSRSHAILQLVVK 427
>AV407795
Length = 424
Score = 61.6 bits (148), Expect = 5e-10
Identities = 45/141 (31%), Positives = 77/141 (53%), Gaps = 6/141 (4%)
Frame = +2
Query: 529 EVYNEQIRDLLATGPASKRLEIKQNYEGHHHVPGVVEAKVDNISDVWTVLQAGSNARAVG 588
E+Y ++ DLL K+L I+++ + + G+ E V ++ + +++ G+ R+ G
Sbjct: 8 EIYGGKLFDLLND---RKKLFIREDGKQQVCIVGLQEYCVSDVESIKELIERGNATRSTG 178
Query: 589 SNNVNEHSSRSHCMLCIMVKTKNLMNGECTK-----SKLWLVDLSGSERLAKTDVQGERL 643
+ NE SSRSH +L + +K ++G +K KL +DL+GSER A T ++
Sbjct: 179 TTGANEESSRSHAILQLAIKRS--VDGNESKPPRPVGKLSFIDLAGSERGADTTDNDKQT 352
Query: 644 K-EAQNINRSLSALGDVISAL 663
+ E IN+SL AL + I AL
Sbjct: 353 RIEGAEINKSLLALKECIRAL 415
>AV775627
Length = 448
Score = 53.1 bits (126), Expect = 2e-07
Identities = 26/47 (55%), Positives = 31/47 (65%)
Frame = -3
Query: 682 QDSLGGDSKTLMFVQISPSDQDVGETLSSLNFATRVRGVELDPVKKQ 728
Q LGGDSKTLMFV ISP V E+L SL FA+RV E+ ++Q
Sbjct: 374 QPCLGGDSKTLMFVNISPDPSSVSESLCSLRFASRVNACEIGSARRQ 234
>AV421105
Length = 415
Score = 47.0 bits (110), Expect = 1e-05
Identities = 30/90 (33%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
Frame = +2
Query: 670 IPYRNSKLTHLLQDSLGGDSKTLMFVQISPSDQDVGETLSSLNFATRVRGV-ELDPVKKQ 728
+PYR SKLT +LQDSLGG S+ LM ++P + E++ +++ A R R + + P +
Sbjct: 5 VPYRESKLTRILQDSLGGTSRALMVACLNPGEYQ--ESVHTVSLAARSRHITNVVPSAHK 178
Query: 729 IDTGELQKTKAMLDKARSECRCKEESLRKL 758
+T ++ +A E + K +S +KL
Sbjct: 179 EETPKVMVDMEAKLRAWLESKGKAKSSQKL 268
>CB827128
Length = 556
Score = 40.4 bits (93), Expect = 0.001
Identities = 42/147 (28%), Positives = 65/147 (43%), Gaps = 3/147 (2%)
Frame = +1
Query: 752 EESLRKLEESLQNIESKAKGKDNIHKNLQEKIKELEGQIKLKTSMQNQSEKQVSQLCERL 811
E + ++E LQ + K+ K+L EK+ LEGQIKL + Q+ + V+
Sbjct: 133 EARILEVESQLQEALQRHAEKEAEAKDLNEKLNALEGQIKLH---EEQAREAVAVSETHK 303
Query: 812 KGKEETCCTLQH---KVKELERKIKEQLQTETANFQQKVCNTFAKLLPTLFSNSLFLPKV 868
G EE+ L+H V EL+ K+ + ETA ++ N+ ++
Sbjct: 304 SGLEESLLKLKHLETVVDELQNKLLHH-EKETAGLNEE--------------NTKLNQEI 438
Query: 869 WDLEKKLKDQLQGSESESSFLKDKVVK 895
E KL D LQ S + KD+ VK
Sbjct: 439 AIYESKLSD-LQSKLSAALVEKDETVK 516
>BP044474
Length = 507
Score = 37.4 bits (85), Expect = 0.011
Identities = 24/72 (33%), Positives = 36/72 (49%), Gaps = 6/72 (8%)
Frame = +1
Query: 102 YP-SIYQSARVGSFS-----YRIDNLPPGQYLVDLHFVEIINVNGPKGMRVFNVYIQEEK 155
YP ++Y+SA + + S Y +D P Y + LHF EI N G RVF++ I +
Sbjct: 163 YPETLYRSALISTSSQPDLTYTLDVDPSKNYSIWLHFAEIDNSVTSTGQRVFDIMINGDV 342
Query: 156 VLSELDIYAAVG 167
++DI G
Sbjct: 343 AFKDVDIVELSG 378
>BP067112
Length = 527
Score = 35.8 bits (81), Expect = 0.031
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = -3
Query: 489 FTMEGTEQNRGVNYRTLEHLFRVSK-ERSETFSYDISVSVLEVYNEQI 535
++ EG+ +RG+ R E LF ++ + + T Y V+V E+YNEQ+
Sbjct: 465 YSQEGSSYDRGLYARCFEELFDLANLDTTSTSQYKFCVTVCELYNEQV 322
>AV778230
Length = 463
Score = 35.8 bits (81), Expect = 0.031
Identities = 18/40 (45%), Positives = 22/40 (55%)
Frame = -2
Query: 692 LMFVQISPSDQDVGETLSSLNFATRVRGVELDPVKKQIDT 731
LMFV ISP GE+L SL FA RV E+ ++ T
Sbjct: 462 LMFVYISPDPSSAGESLCSLRFAARVHACEIGIPRRPTST 343
>BP037078
Length = 529
Score = 34.3 bits (77), Expect = 0.090
Identities = 29/116 (25%), Positives = 60/116 (51%), Gaps = 1/116 (0%)
Frame = +1
Query: 720 VELDPVKKQIDTGELQKTKAMLDKARSECRCKEESLRKLEESLQNIESKAKGKDNIHKNL 779
++LD + +I T E Q + DK++ E + K+E + + E+ +Q+ S + N +L
Sbjct: 154 IQLDQLNSRILTLESQ----IKDKSQ-ELKKKDEVIAEKEKFIQDKSSTVESLQNEVASL 318
Query: 780 QEKIK-ELEGQIKLKTSMQNQSEKQVSQLCERLKGKEETCCTLQHKVKELERKIKE 834
Q+K + + ++ + + +KQV L L+ + + + +V ELE+KI +
Sbjct: 319 QKKGSLDAQEEVGKAHARAGELQKQVENLKRELEKQNKEKVNWETRVAELEKKIHD 486
>BP049886
Length = 541
Score = 30.4 bits (67), Expect = 1.3
Identities = 13/40 (32%), Positives = 20/40 (49%)
Frame = -1
Query: 62 VNVGGEATNEGADGVKFLSDTFFDGGDVFLTNEAIVEGGD 101
+N+GGE N+G +S+ FDG V A + G+
Sbjct: 166 INIGGEGLNDGGSAALEISEDLFDGAGVVAFVHAWIWNGE 47
>AI967308
Length = 443
Score = 30.0 bits (66), Expect = 1.7
Identities = 28/111 (25%), Positives = 46/111 (41%)
Frame = +1
Query: 723 DPVKKQIDTGELQKTKAMLDKARSECRCKEESLRKLEESLQNIESKAKGKDNIHKNLQEK 782
D ++K+ E T D + E C +E K +E + NL+EK
Sbjct: 115 DQIEKETQPSEDSTTPP--DAPKPEIPCVDEMTLKNDEIAL-----------LKCNLEEK 255
Query: 783 IKELEGQIKLKTSMQNQSEKQVSQLCERLKGKEETCCTLQHKVKELERKIK 833
+ ELE K +++ Q + VS++ E +E L+ +ELE K
Sbjct: 256 VTELESMSKENENLKQQLNEAVSKVSEAETKEEGMKLQLKELGEELEAASK 408
>BI417263
Length = 539
Score = 29.6 bits (65), Expect = 2.2
Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 723 DPVKKQIDTGELQKTKAMLDKARS-ECRCKEESLRKLEESLQNIESKAKGK 772
+ +KKQID E+ K LDKAR + R +++L + + + E K K K
Sbjct: 260 EQLKKQIDNLEMMKADGALDKARKHKKRQLQDTLNLVVKKRREYEDKMKEK 412
>TC13753 similar to UP|Q9LQQ6 (Q9LQQ6) F24B9.6, partial (38%)
Length = 614
Score = 28.5 bits (62), Expect = 5.0
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 798 NQSEKQVSQLCERLKGKEETCCTLQHKVKELERKIKE 834
+ EK+ S L LK +E ++HK++ L K+KE
Sbjct: 255 DSKEKEASPLAALLKEMKEGLDVVRHKIQSLTAKVKE 365
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.132 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,691,879
Number of Sequences: 28460
Number of extensions: 146303
Number of successful extensions: 735
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 727
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 730
length of query: 895
length of database: 4,897,600
effective HSP length: 98
effective length of query: 797
effective length of database: 2,108,520
effective search space: 1680490440
effective search space used: 1680490440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146711.13