BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146709.5 + phase: 0 /pseudo
(1304 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8301 75 7e-14
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 44 1e-04
TC8302 40 0.002
AV778782 40 0.003
TC9039 39 0.004
BP065763 37 0.026
BI418821 36 0.045
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 35 0.10
BP054557 34 0.13
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 34 0.13
AV421607 34 0.17
AV773507 34 0.17
AV420911 32 0.50
TC12416 similar to GB|AAH56066.1|33417132|BC056066 sop-prov prot... 32 0.50
TC12440 30 1.9
TC16853 similar to UP|Q93ZP3 (Q93ZP3) At1g32359/F27G20.10, parti... 30 3.2
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 29 4.2
AV770138 29 5.5
BP039068 28 7.2
TC12773 28 9.4
>TC8301
Length = 1243
Score = 75.1 bits (183), Expect = 7e-14
Identities = 42/105 (40%), Positives = 66/105 (62%)
Frame = +2
Query: 2 MGSKWDIEKFTGSNDFGLWKVKMRAILIQQKCVEALKGEAQMSAHLTPAEKTEMNDKAVS 61
M +K++I KF+GSN F LWK+K++AIL + C+ A+ G A +T + EM+D AV+
Sbjct: 500 MAAKFEIPKFSGSN-FSLWKLKIKAILRKDNCLPAIDGRP---ADITDDKWKEMDDNAVA 667
Query: 62 AIILCLGDKVLREVSREATAVSMWNKLDSLYMTKSLAHRQCLKQQ 106
+ L + D VL ++ + TA +W+ L LY KSL H + LK++
Sbjct: 668 NLHLAVADSVLSSIAEKKTAKEIWDTLIQLYEVKSL-HNRILKEE 799
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 44.3 bits (103), Expect = 1e-04
Identities = 20/61 (32%), Positives = 27/61 (43%)
Frame = +1
Query: 195 DSGEGLNISRGRSHNKGKGKGKNSRSKSRSKGDGNKTQYKCFICHNPGHFKKDCPERKDN 254
D G + + G G G R RS G G + KC+ C PGHF ++C R +
Sbjct: 259 DGKNGWRVELSHNSRGGGGGGGGGRGGGRSGGGGGGSDMKCYECGEPGHFARECRMRGGS 438
Query: 255 G 255
G
Sbjct: 439 G 441
>TC8302
Length = 494
Score = 40.4 bits (93), Expect = 0.002
Identities = 37/148 (25%), Positives = 60/148 (40%), Gaps = 4/148 (2%)
Frame = +3
Query: 105 QQLYFYRMVESKPIMEQLTEFNKIIDDLANIDVNLEDEDKVLHLLCALPRSFE----NFK 160
++LY RM ES + + + N + L+ + + + ++ LL +LP S++ N K
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 161 DTMLYGKEGTITLEEVQAALRTKELTKFKELKVDDSGEGLNISRGRSHNKGKGKGKNSRS 220
+ + + L EV ++ K E L ++RGRS
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR*NKEDR*DSSKPMEALTMTRGRS------------- 323
Query: 221 KSRSKGDGNKTQYKCFICHNPGHFKKDC 248
KSR K KC+ C H KKDC
Sbjct: 324 KSRKK-----INLKCYHCGQR*HLKKDC 392
>AV778782
Length = 351
Score = 39.7 bits (91), Expect = 0.003
Identities = 20/41 (48%), Positives = 27/41 (65%)
Frame = -1
Query: 1260 TSSET*LRLRRSW*RKWHRKTIQQTCSPNHYQDQDSSIAWT 1300
T SE L+L + +K+ R IQQTC P+H+Q SS+AWT
Sbjct: 342 TLSEILLQLVKL*LKKFTRMRIQQTCLPSHFQMSSSSLAWT 220
>TC9039
Length = 1218
Score = 39.3 bits (90), Expect = 0.004
Identities = 39/163 (23%), Positives = 68/163 (40%), Gaps = 1/163 (0%)
Frame = +2
Query: 87 KLDSLYMTKSLAHRQCLKQQLYFYRMVESKPIMEQLTEFNKIIDDLANIDVNLEDEDKVL 146
+++ + A L Q L + I E + + I L + + L D D ++
Sbjct: 413 EIEKRFAKSDKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELSD-DLLI 589
Query: 147 HL-LCALPRSFENFKDTMLYGKEGTITLEEVQAALRTKELTKFKELKVDDSGEGLNISRG 205
HL L +LP F FK + KE E + ++ +E K +E K +S ++ S+
Sbjct: 590 HLVLLSLPAQFSQFKISYNCPKEKWSLNELISFCVQEEERLK-QERK--ESAHFVSTSKD 760
Query: 206 RSHNKGKGKGKNSRSKSRSKGDGNKTQYKCFICHNPGHFKKDC 248
+ K + KN + + + K C+ C+ GH KK C
Sbjct: 761 KGKRKKTVEPKNEAADAPAPKK-QKEDDTCYFCNVSGHMKKKC 886
Score = 32.0 bits (71), Expect = 0.65
Identities = 15/49 (30%), Positives = 27/49 (54%)
Frame = +3
Query: 315 VRLGNNKACKIQGMGTIRLKMFDDRDFLLKNVRYIPELKRNLISISMFD 363
+ +G+ K +++ +G RL + LK+ +P +RNLIS+S D
Sbjct: 960 IYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLD 1106
>BP065763
Length = 537
Score = 36.6 bits (83), Expect = 0.026
Identities = 20/63 (31%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Frame = -1
Query: 192 KVDDSGEGLNISRGRSHNKGK-GKGKNSRSKSRSKGDGN-KTQYKCFICHNPGHFKKDCP 249
K D L G S +K K G+ + + ++ +G ++ C+ C GHFKK CP
Sbjct: 516 KEKDHSIHLTTHDGASXSKAKPGRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKYCP 337
Query: 250 ERK 252
+RK
Sbjct: 336 KRK 328
Score = 29.3 bits (64), Expect = 4.2
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2
Query: 317 LGNNKACKIQGMGTIRLKMFDDRDFLLKNVRYIPELKRNLIS 358
+GN +I+G+GT RL + L+ Y+ RNL+S
Sbjct: 260 MGNQMKARIEGIGTYRLILDTGHQLDLEKCLYVRGCARNLVS 135
>BI418821
Length = 614
Score = 35.8 bits (81), Expect = 0.045
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 1/83 (1%)
Frame = +2
Query: 176 VQAALRTKELTKFKELKVDD-SGEGLNISRGRSHNKGKGKGKNSRSKSRSKGDGNKTQYK 234
V+ ++ T + K K + V +G L +R S +G G + + R+ G G
Sbjct: 218 VEFSIATGDNDKTKAVDVTGPNGAALQPTRKDSAPRGFGGWRGG--ERRNGGGGGGGGGG 391
Query: 235 CFICHNPGHFKKDCPERKDNGGG 257
C+ C + GH +DC +NGGG
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGG 460
Score = 30.0 bits (66), Expect = 2.5
Identities = 13/39 (33%), Positives = 18/39 (45%)
Frame = +2
Query: 219 RSKSRSKGDGNKTQYKCFICHNPGHFKKDCPERKDNGGG 257
RS + G G C+ C + GH +DC +N GG
Sbjct: 437 RSNNNGGGGGGAA---CYNCGDAGHLARDCNRSNNNSGG 544
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 34.7 bits (78), Expect = 0.10
Identities = 18/55 (32%), Positives = 26/55 (46%)
Frame = +2
Query: 204 RGRSHNKGKGKGKNSRSKSRSKGDGNKTQYKCFICHNPGHFKKDCPERKDNGGGE 258
RGR + +G SR S+G +CF C PGHF ++CP + G+
Sbjct: 338 RGRDRDD-RGDRDRSRGYGGSRGSNGG---ECFKCGKPGHFARECPSEGETRRGK 490
>BP054557
Length = 538
Score = 34.3 bits (77), Expect = 0.13
Identities = 20/73 (27%), Positives = 36/73 (48%)
Frame = -2
Query: 288 VLDSGCSYHICPRKEYFETLELKEGGVVRLGNNKACKIQGMGTIRLKMFDDRDFLLKNVR 347
+LD+G ++H+C + F+ L ++ + N + +I M ++ F L V
Sbjct: 537 LLDTGATHHVCHNLDVFQALRQVHPVLLSMPNGQK-EIAHMAD---SVYFSEKFFLAXVL 370
Query: 348 YIPELKRNLISIS 360
Y+P NLIS+S
Sbjct: 369 YVPSFNFNLISVS 331
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 34.3 bits (77), Expect = 0.13
Identities = 17/50 (34%), Positives = 22/50 (44%)
Frame = +1
Query: 207 SHNKGKGKGKNSRSKSRSKGDGNKTQYKCFICHNPGHFKKDCPERKDNGG 256
SHN G G + R D KC+ C PGHF ++C R + G
Sbjct: 97 SHNSKGGGGGRGGGRGRGGED-----LKCYECGEPGHFARECRSRGGSRG 231
>AV421607
Length = 245
Score = 33.9 bits (76), Expect = 0.17
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = +3
Query: 226 GDGNKTQYKCFICHNPGHFKKDCPERKDN 254
G G+ T C+ C PGH+ +DCP N
Sbjct: 27 GSGSGTATGCYKCGRPGHWSRDCPSSAPN 113
>AV773507
Length = 496
Score = 33.9 bits (76), Expect = 0.17
Identities = 27/83 (32%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
Frame = +2
Query: 194 DDSGEGLNISRGRSHNKGKGKGKNSRSKSRSKGDGNKT-QYKCFICHNPGHFKKDCPERK 252
DD+ +G RG ++ G G+G S G G++ Q CF C PGH +DCP
Sbjct: 260 DDADQGY---RGGGYSSG-GRG--------SYGAGDRVGQDDCFECGRPGHRARDCPLAG 403
Query: 253 DNGG-GESSVQIASKDEGYESAG 274
GG G ++S+ S G
Sbjct: 404 GGGGRGRGGGSLSSRPRYGGSGG 472
>AV420911
Length = 418
Score = 32.3 bits (72), Expect = 0.50
Identities = 15/42 (35%), Positives = 19/42 (44%)
Frame = +2
Query: 207 SHNKGKGKGKNSRSKSRSKGDGNKTQYKCFICHNPGHFKKDC 248
SHN G G + R D KC+ C PGHF ++C
Sbjct: 305 SHNSKGGGGGRGGGRGRGGED-----LKCYECGEPGHFAREC 415
>TC12416 similar to GB|AAH56066.1|33417132|BC056066 sop-prov protein
{Xenopus laevis;} , partial (32%)
Length = 416
Score = 32.3 bits (72), Expect = 0.50
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +3
Query: 194 DDSGEGLNISRGRSHNKGKGKGKNSRSKSRSKGDGNKTQY 233
D G G RGR +G+G+G+ R + +GDG+K ++
Sbjct: 132 DGFGRGRGEGRGRGEGRGRGRGR-GRGRRGRRGDGDKKEW 248
>TC12440
Length = 629
Score = 30.4 bits (67), Expect = 1.9
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -1
Query: 1247 CIMRGQSTLTFVCTSSET*LRLRRSW*RKWHRKT 1280
C++ Q+ L SSET RLRR W W R+T
Sbjct: 278 CLVGEQTLLPTTRFSSETATRLRRRWAPPWRRRT 177
>TC16853 similar to UP|Q93ZP3 (Q93ZP3) At1g32359/F27G20.10, partial (20%)
Length = 722
Score = 29.6 bits (65), Expect = 3.2
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 10/54 (18%)
Frame = -2
Query: 316 RLGNNKACKIQGMGTIRLKMFDDRDFLLKN----------VRYIPELKRNLISI 359
+LG+ K +IQG T R + R +LLK +RYIPEL + L+ +
Sbjct: 694 QLGDTKLVRIQGADTFRRQ----RTYLLKR*TLPPNIKAQIRYIPELNKVLLGL 545
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 29.3 bits (64), Expect = 4.2
Identities = 20/67 (29%), Positives = 27/67 (39%), Gaps = 6/67 (8%)
Frame = +2
Query: 189 KELKVDDSGEGLNISRGRSHNKGKGKGKNSRSK------SRSKGDGNKTQYKCFICHNPG 242
KE K S + + SR RS + K ++ R R G C C PG
Sbjct: 188 KEEKRKMSSDSRSRSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPG 367
Query: 243 HFKKDCP 249
HF ++CP
Sbjct: 368 HFARECP 388
>AV770138
Length = 483
Score = 28.9 bits (63), Expect = 5.5
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -1
Query: 1223 WGLVKDV*RYIVIAKVPFIWQIIRCIMRGQSTLTFVCTSS 1262
W L * Y+++ + +WQ++RCI+ S F C S
Sbjct: 363 WALTSAA*VYLLLFSILRLWQLVRCIVPA*S--AFCCADS 250
>BP039068
Length = 467
Score = 28.5 bits (62), Expect = 7.2
Identities = 17/57 (29%), Positives = 21/57 (36%), Gaps = 11/57 (19%)
Frame = +3
Query: 220 SKSRSKGDGNKTQYKCFICHNPGHFKKDCPE-----------RKDNGGGESSVQIAS 265
S S + N Q C C GH DCP R + GE+SV +S
Sbjct: 267 STSSNVNHNNPRQTVCMNCQETGHASNDCPSLTLRSRNAQHPRMNQQSGETSVSCSS 437
>TC12773
Length = 420
Score = 28.1 bits (61), Expect = 9.4
Identities = 12/41 (29%), Positives = 19/41 (46%)
Frame = +2
Query: 208 HNKGKGKGKNSRSKSRSKGDGNKTQYKCFICHNPGHFKKDC 248
HN+ + K S + + Y+C+ C PGH +DC
Sbjct: 296 HNRHRWSNKRS----------SLSTYECWKCQRPGHMAEDC 388
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.357 0.158 0.567
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,628,698
Number of Sequences: 28460
Number of extensions: 331225
Number of successful extensions: 3459
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 2560
Number of HSP's successfully gapped in prelim test: 79
Number of HSP's that attempted gapping in prelim test: 808
Number of HSP's gapped (non-prelim): 2734
length of query: 1304
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1203
effective length of database: 2,023,140
effective search space: 2433837420
effective search space used: 2433837420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146709.5