BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146706.7 + phase: 0 /pseudo
(617 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14969 similar to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (cl... 89 3e-18
AV427444 73 1e-13
TC7805 homologue to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (c... 60 8e-10
TC19828 similar to UP|Q9FNT6 (Q9FNT6) Long chain acyl-CoA synthe... 60 8e-10
TC15125 weakly similar to UP|Q84P20 (Q84P20) Acyl-activating enz... 47 1e-05
TC15126 similar to UP|Q84P19 (Q84P19) Acyl-activating enzyme 11,... 41 5e-04
TC9417 weakly similar to UP|Q9S834 (Q9S834) ATP-dependent Clp pr... 41 5e-04
TC17423 similar to PIR|H85064|H85064 4-coumarate-CoA ligase-like... 41 6e-04
AW719487 39 0.002
BP043696 39 0.002
AV778309 39 0.003
AV772740 38 0.004
AU251967 37 0.007
TC11842 similar to UP|Q8SX80 (Q8SX80) LD21711p, partial (3%) 37 0.007
TC18806 similar to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A lig... 35 0.046
AV409031 34 0.061
AV424050 33 0.10
BP058100 33 0.13
TC10339 homologue to UP|RL24_CICAR (O65743) 60S ribosomal protei... 33 0.13
TC15357 similar to UP|Q9ZV06 (Q9ZV06) At2g39020 protein, partial... 30 1.5
>TC14969 similar to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (clone
GM4CL1B) - soybean (fragment) {Glycine
max;} , partial (70%)
Length = 928
Score = 88.6 bits (218), Expect = 3e-18
Identities = 80/302 (26%), Positives = 125/302 (40%), Gaps = 26/302 (8%)
Frame = +2
Query: 280 IKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAAS------------LACI*KSL* 327
I +D L ++SGTTG PKGV+LTH++L + + L C+ L
Sbjct: 185 INPEDAVALPFSSGTTGLPKGVVLTHKSLTTSVGQQVDGENPNLSLGTEDVLLCV---LP 355
Query: 328 VFHFF---------MRC*PSIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTFIRVSL 378
+FH F +R + K E+ L + ++S + +V LV
Sbjct: 356 LFHIFSLNSVLLCALRAGSGVLLMPKFEIGSLLELIQKHRVSVAMVVPPLVL-------- 511
Query: 379 GYMECKRIYEGKCLTKNQKSPSYLYAMLDWLGARIIATILFPIHMLAKKLVYSKIHSAIG 438
L KN K A D R++ + P+ ++ + S++ A+
Sbjct: 512 ------------ALAKNPK-----VAEFDLSSIRLVLSGAAPLGKELEEALRSRVPQAV- 637
Query: 439 FSKAGISGGGSLPSHVDRFFEAIGVTLQNGYGLTETSPVIA-----ARRLSCNVIGSVGH 493
L GYG+TE PV++ A++ GS G
Sbjct: 638 --------------------------LGQGYGMTEAGPVLSMSLGFAKQPFPTKSGSCGT 739
Query: 494 PLKHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIG 553
+++ E KV+D ETG L G + +RG Q+MKGY + AT ID +GWL+TGD+G
Sbjct: 740 VVRNAELKVLDPETGCSLGYNQPGEICIRGQQIMKGYLNDEKATATTIDAEGWLHTGDVG 919
Query: 554 WI 555
+I
Sbjct: 920 YI 925
>AV427444
Length = 428
Score = 73.2 bits (178), Expect = 1e-13
Identities = 44/117 (37%), Positives = 63/117 (53%), Gaps = 5/117 (4%)
Frame = +1
Query: 444 ISGGGSLPSHVDRFFE-AIGVTLQNGYGLTETSPVIAARRLSCNVIGSVGHPLKHTEFKV 502
+SG L + F + G + GYG+TET+ VI+ + G VG P E K+
Sbjct: 25 VSGASPLSPDIMEFLKICFGGRVTEGYGMTETTCVISCIDQGDKLGGHVGSPSPACEVKL 204
Query: 503 VDSETGEVLP---PGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIG-WI 555
VD P +G + VRGP + KGYYK+ + T + ID++GWL+TGDIG W+
Sbjct: 205 VDVPEMNYTSDDQPQPRGEICVRGPIIFKGYYKDEAQTREVIDEEGWLHTGDIGTWL 375
>TC7805 homologue to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (clone
GM4CL1B) - soybean (fragment) {Glycine
max;} , partial (41%)
Length = 965
Score = 60.5 bits (145), Expect = 8e-10
Identities = 28/60 (46%), Positives = 39/60 (64%)
Frame = +3
Query: 496 KHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
++ E KV+D ETG L G + +RG Q+MKGY + AT ID +GWL+TGD+G+I
Sbjct: 90 RNAELKVLDPETGCSLGYNQPGEICIRGQQIMKGYLNDEKATATTIDAEGWLHTGDVGYI 269
>TC19828 similar to UP|Q9FNT6 (Q9FNT6) Long chain acyl-CoA synthetase ,
partial (17%)
Length = 378
Score = 60.5 bits (145), Expect = 8e-10
Identities = 41/118 (34%), Positives = 57/118 (47%), Gaps = 8/118 (6%)
Frame = +1
Query: 444 ISGGGSLPSHVDRFFE-AIGVTLQNGYGLTETSPVIAARRLSCNVIGSVGHPLKHTEFKV 502
+SGG L + RF +G + GYGLTET +G VG PL + K+
Sbjct: 16 LSGGAPLSADTQRFINICLGAPIGQGYGLTETCAGGTFSDFDDTSVGRVGPPLPCSYIKL 195
Query: 503 VDSETGEVLP---PGYKGILKVRGPQLMKGYYKNPSATNQA--IDKDG--WLNTGDIG 553
+D G P +G + V GP + GY+KN TN++ +D+ G W TGDIG
Sbjct: 196 IDWPEGGYSTNDSPMPRGEIVVGGPNVTLGYFKNEEKTNESYKVDERGVRWFYTGDIG 369
>TC15125 weakly similar to UP|Q84P20 (Q84P20) Acyl-activating enzyme 12,
partial (48%)
Length = 1164
Score = 46.6 bits (109), Expect = 1e-05
Identities = 48/206 (23%), Positives = 92/206 (44%), Gaps = 6/206 (2%)
Frame = +1
Query: 107 RSSAEKYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCR 166
+ +A Y +++++I H+ T+ Q + A LR + ++ N+ +++ A N
Sbjct: 163 KRAAASYPNRVSVI---HEG-IRFTWSQTYERCRRLAFTLRSLNIAKNDVVSVLAPNIPA 330
Query: 167 WLVADQGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASM 226
+ GA+ SR + + I +HSE+ VD + + KA + ++
Sbjct: 331 MYEMHFAVPMAGAVLNTINSRLDAKNIATILSHSEAKIFFVD----YEYVNKA---REAL 489
Query: 227 RFVILLWGEKSCLVNEGSKEVPIFTFTEIMHLGRGSRR---LFES--HDARKHYVFEAIK 281
R +++ G K + +P+ + ++ G R +E H +YV E I+
Sbjct: 490 RMLVVQKGVKPNSTEQDYSTLPLVIVIDDINSPTGVRLGELEYEQMVHRGNPNYVPEEIQ 669
Query: 282 SD-DIATLVYTSGTTGNPKGVMLTHQ 306
+ L YTSGTT PKGV+ +H+
Sbjct: 670 EEWSPIALNYTSGTTSEPKGVVYSHR 747
>TC15126 similar to UP|Q84P19 (Q84P19) Acyl-activating enzyme 11, partial
(25%)
Length = 749
Score = 41.2 bits (95), Expect = 5e-04
Identities = 40/182 (21%), Positives = 78/182 (41%), Gaps = 6/182 (3%)
Frame = +2
Query: 131 TYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSSI 190
T+ Q D A LR + ++ N+ +++ A N + GA+ +R
Sbjct: 209 TWSQTYDRCRRLAFSLRSLNIARNDVVSVLAPNIPAMYEMHFAVPMAGAVLNTINTRLDA 388
Query: 191 EELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVILLWGEKSCLVNEGSKEVPIF 250
+ + I HSE+ VD + + KA + +++ +++ G + + +P+
Sbjct: 389 KNIATILRHSEAKVFFVD----YEYVNKA---REALKMLVVQKGAEPNPKEQNYATLPLV 547
Query: 251 TFTEIMHLGRGSRRLFESHD-----ARKHYVFEAIKSD-DIATLVYTSGTTGNPKGVMLT 304
+ ++ G R ++ +YV E I+ + L YTSGTT PKGV+ +
Sbjct: 548 IVIDDINSPTGVRLGELEYEQMVQHGNPNYVPEEIQEEWSPMALNYTSGTTSEPKGVVYS 727
Query: 305 HQ 306
H+
Sbjct: 728 HR 733
>TC9417 weakly similar to UP|Q9S834 (Q9S834) ATP-dependent Clp protease
subunit ClpP (ATP-dependent Clp protease proteolytic
subunit ClpP5) (Endopeptidase Clp) , partial (14%)
Length = 466
Score = 41.2 bits (95), Expect = 5e-04
Identities = 34/107 (31%), Positives = 47/107 (43%), Gaps = 17/107 (15%)
Frame = -3
Query: 12 SNHHFFFFFFFFFYK---------------VKCQRFPSLPNATTILLQPSSPILFLSSFL 56
S H FFFFFF FF + ++ PSL A T L P+S + FL S
Sbjct: 452 STHSFFFFFFIFFCRSIHNKRSLEIHIDR*IQIASSPSLTMAHTSLFTPTSSLTFLPSLP 273
Query: 57 ATASPLLSTFLELVSFANLNPRFSPLLESSLL--SGNDGVVSDEWKT 101
+++S S L L S + L+ + ES + S V DE+ T
Sbjct: 272 SSSSSSRSVNLSLPSHSFLSGKLRKSAESKVTKKSPVKAVYGDEFWT 132
>TC17423 similar to PIR|H85064|H85064 4-coumarate-CoA ligase-like protein
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (25%)
Length = 655
Score = 40.8 bits (94), Expect = 6e-04
Identities = 14/26 (53%), Positives = 20/26 (76%)
Frame = +1
Query: 529 GYYKNPSATNQAIDKDGWLNTGDIGW 554
GY+ NP AT +DK GW++TGD+G+
Sbjct: 16 GYHNNPEATKLTLDKKGWVHTGDVGY 93
>AW719487
Length = 498
Score = 39.3 bits (90), Expect = 0.002
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = +2
Query: 533 NPSATNQAIDKDGWLNTGDIGWI 555
+P AT + IDK+GWL+TGDIG+I
Sbjct: 5 DPEATERTIDKEGWLHTGDIGYI 73
>BP043696
Length = 470
Score = 38.9 bits (89), Expect = 0.002
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = +1
Query: 130 MTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVADQG 173
++Y + +A+ FA GL+ +G E++A+FAD W +A QG
Sbjct: 313 LSYDAVFEAVTSFASGLKELGHGREERVAIFADTREEWFIALQG 444
>AV778309
Length = 543
Score = 38.5 bits (88), Expect = 0.003
Identities = 24/96 (25%), Positives = 47/96 (48%), Gaps = 3/96 (3%)
Frame = +2
Query: 130 MTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSS 189
+TYK+ DA + + GV+P ++ ++ N W++A + + G V
Sbjct: 254 VTYKEAYDAAIRMGSAMNSCGVNPGDRCGIYGSNCPEWIMAMEACNSYGVTYVPLYDTLG 433
Query: 190 IEELLQIYNHSE-SIALAVDN--PEMFNRIAKAFDL 222
+ + I NH+E SIAL D+ P +++ + + +L
Sbjct: 434 PKAVEFIINHAEVSIALVQDSKIPSIWSCLGRCSNL 541
>AV772740
Length = 555
Score = 38.1 bits (87), Expect = 0.004
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = -2
Query: 284 DIATLVYTSGTTGNPKGVMLTHQNL 308
+I T++YTSGTTG P V+LTH N+
Sbjct: 329 NICTIMYTSGTTGTPSAVVLTHANI 255
>AU251967
Length = 380
Score = 37.4 bits (85), Expect = 0.007
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = +2
Query: 16 FFFFFFFFFYKVKCQRFPSLPNATTILLQPSSPILFLSSFLATASPLLST 65
FFFFFFFFF ++ R + ++LL P+ +L L L SP+ T
Sbjct: 104 FFFFFFFFFLRITQTRNHNSQFTVSLLLLPTHTLLLLLLLLLLNSPMAMT 253
>TC11842 similar to UP|Q8SX80 (Q8SX80) LD21711p, partial (3%)
Length = 557
Score = 37.4 bits (85), Expect = 0.007
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = -2
Query: 16 FFFFFFFFFYKVKCQRFPSLPNATTILLQPSSPILFLSSFLATASPLLST 65
FFFFFFFFF ++ R + ++LL P+ +L L L SP+ T
Sbjct: 445 FFFFFFFFFLRITQTRNHNSQFTVSLLLLPTHTLLLLLLLLLLNSPMAMT 296
>TC18806 similar to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A ligase ,
partial (26%)
Length = 529
Score = 34.7 bits (78), Expect = 0.046
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
Frame = +1
Query: 465 LQNGYGLTETSPVIAARRLSCNVI----GSVGHPLKHTEFKVVDSETGEVLP 512
L GYG+TE P+ + + G+ G +++ E K+VD+ETG LP
Sbjct: 259 LGQGYGMTEAGPLAISLAFAKEPFKTKSGACGTVVRNAEMKIVDTETGASLP 414
>AV409031
Length = 343
Score = 34.3 bits (77), Expect = 0.061
Identities = 15/29 (51%), Positives = 19/29 (64%)
Frame = +3
Query: 284 DIATLVYTSGTTGNPKGVMLTHQNLLHQI 312
DIA ++TSGTT PKGV L+ NL +
Sbjct: 117 DIALFLHTSGTTSRPKGVPLSQHNLASSV 203
>AV424050
Length = 274
Score = 33.5 bits (75), Expect = 0.10
Identities = 14/36 (38%), Positives = 22/36 (60%)
Frame = +1
Query: 16 FFFFFFFFFYKVKCQRFPSLPNATTILLQPSSPILF 51
FFFFFFFFF+ Q P+ P + + + + +S L+
Sbjct: 46 FFFFFFFFFFFFYSQTHPASPPSPSTMAEQTSKSLY 153
>BP058100
Length = 457
Score = 33.1 bits (74), Expect = 0.13
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -3
Query: 8 HLFKSNHHFFFFFFFFFYKVKCQRFPSLPNATTILLQPSSPILFLSSFLATASPLLSTFL 67
HL HH K + P+ P +T+ QPS+ I + +L TA+ L S +L
Sbjct: 314 HLLADKHHILS*RIDLLQK*HLMKLPTKPFESTVRYQPSTAISTVGFYLTTATTLASKYL 135
>TC10339 homologue to UP|RL24_CICAR (O65743) 60S ribosomal protein L24,
complete
Length = 677
Score = 33.1 bits (74), Expect = 0.13
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = -2
Query: 6 STHLFKSNHHFFFFFFFFFYKVKC 29
S L NH FFFFFFFFF + C
Sbjct: 100 SAKLCLENHGFFFFFFFFFSSLCC 29
>TC15357 similar to UP|Q9ZV06 (Q9ZV06) At2g39020 protein, partial (69%)
Length = 1578
Score = 29.6 bits (65), Expect = 1.5
Identities = 18/48 (37%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Frame = -1
Query: 16 FFFFFFFFF---YKVKCQRFPSLPNATTILLQPSSPILFLSSFLATAS 60
FFFFFFFFF + F ++ +T I S+ F FL AS
Sbjct: 321 FFFFFFFFFSDLWSSTASPFVTVGLSTAISSPSSAAFCFFLDFLLEAS 178
Score = 29.6 bits (65), Expect = 1.5
Identities = 20/51 (39%), Positives = 25/51 (48%)
Frame = -3
Query: 16 FFFFFFFFFYKVKCQRFPSLPNATTILLQPSSPILFLSSFLATASPLLSTF 66
FFFFFFFFF+ ++ N I S I F+SSFL L +F
Sbjct: 322 FFFFFFFFFF*FMVLHCITICNCWLI---NSHLITFISSFLLLLGFLARSF 179
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.140 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,747,399
Number of Sequences: 28460
Number of extensions: 153525
Number of successful extensions: 2760
Number of sequences better than 10.0: 120
Number of HSP's better than 10.0 without gapping: 1674
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2199
length of query: 617
length of database: 4,897,600
effective HSP length: 96
effective length of query: 521
effective length of database: 2,165,440
effective search space: 1128194240
effective search space used: 1128194240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146706.7