BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146683.3 + phase: 0 /pseudo
(122 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB829481 36 0.002
TC17338 29 0.20
BP073030 26 1.7
TC14642 homologue to UP|O65761 (O65761) Beta-galactosidase (Lac... 26 2.2
TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana ... 25 2.8
TC10972 similar to UP|Q40318 (Q40318) Coil protein, partial (32%) 25 2.8
TC15299 similar to UP|Q84MJ5 (Q84MJ5) Methylmalonate semialdehyd... 25 3.7
BI420759 25 3.7
BP065061 25 3.7
TC17880 similar to GB|AAN28894.1|23506069|AY143955 At2g28370/T1B... 25 4.8
TC15598 similar to UP|Q7XZI4 (Q7XZI4) DP1, partial (13%) 25 4.8
TC12063 similar to UP|HIS5_ARATH (Q9SZ30) Imidazole glycerol pho... 25 4.8
AW719590 25 4.8
TC10261 homologue to UP|DAG_ANTMA (Q38732) DAG protein, chloropl... 25 4.8
TC14864 homologue to UP|AAN77867 (AAN77867) 5-enolpyruvylshikima... 24 6.3
TC9890 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F13M... 24 6.3
TC15489 similar to UP|DDL_BACSU (P96612) D-alanine--D-alanine li... 24 8.3
>CB829481
Length = 576
Score = 36.2 bits (82), Expect = 0.002
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 5/51 (9%)
Frame = +2
Query: 54 VAFSAALGLLECCFVNTGSRIMVF-----ASGPGLVLDSDFWQSMRTHNDI 99
V S A GLL C TG+RI+ GPG ++ D +R+H D+
Sbjct: 8 VGLSVAAGLLSACNPGTGARIIALVGGPCTEGPGTIVSKDLSDPVRSHKDL 160
>TC17338
Length = 762
Score = 29.3 bits (64), Expect = 0.20
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -1
Query: 96 HNDIYICNGHLAVYTSKLLRGCRCVCC 122
HN I HL VY+ K L+ C C C
Sbjct: 711 HNSTCILTQHLKVYSEKKLKFCNCCNC 631
>BP073030
Length = 333
Score = 26.2 bits (56), Expect = 1.7
Identities = 9/24 (37%), Positives = 13/24 (53%)
Frame = -3
Query: 90 WQSMRTHNDIYICNGHLAVYTSKL 113
W + +NDIY CN ++ KL
Sbjct: 310 WLHLEVYNDIYFCNFRCSIIHKKL 239
>TC14642 homologue to UP|O65761 (O65761) Beta-galactosidase (Lactase)
(Fragment) , partial (78%)
Length = 2239
Score = 25.8 bits (55), Expect = 2.2
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = -3
Query: 47 SRNRSTGVAFSAALGLLECCFVNT 70
S NR+TG ALG CC +N+
Sbjct: 443 SMNRNTGHLHLCALGTNPCCVINS 372
>TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana large
subunit 26S ribosomal RNA gene, partial sequence,
complete
Length = 6006
Score = 25.4 bits (54), Expect = 2.8
Identities = 16/44 (36%), Positives = 22/44 (49%)
Frame = -1
Query: 47 SRNRSTGVAFSAALGLLECCFVNTGSRIMVFASGPGLVLDSDFW 90
S N GV SA LG + V+ S +++ +GP LDS W
Sbjct: 3816 STNTLCGV*VSAQLGTVTQLPVHPASPVLLTKNGPLGALDSVVW 3685
>TC10972 similar to UP|Q40318 (Q40318) Coil protein, partial (32%)
Length = 548
Score = 25.4 bits (54), Expect = 2.8
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = +1
Query: 41 STYNFKSRNRSTGVAFSAALGLLECCFVNTGSRIMVFASGPGLVLD 86
+ YNF N +TGV ++A GL ++ + AS L++D
Sbjct: 220 AVYNFGDSNSNTGVVYAAFAGLQSPGGISFFGNLSGRASDGRLIID 357
>TC15299 similar to UP|Q84MJ5 (Q84MJ5) Methylmalonate semialdehyde
dehydrogenase (Fragment), partial (31%)
Length = 570
Score = 25.0 bits (53), Expect = 3.7
Identities = 14/46 (30%), Positives = 19/46 (40%), Gaps = 6/46 (13%)
Frame = +2
Query: 82 GLVLDSDFWQSMRTHNDIYICNGHLAVYTSKLLRGC------RCVC 121
G++ DFW S TH GH ++ + C RCVC
Sbjct: 32 GVLQGGDFWSSSSTHGVR*FGEGHKHCQ*KQVWKWC*YIYCFRCVC 169
>BI420759
Length = 433
Score = 25.0 bits (53), Expect = 3.7
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = -3
Query: 23 TNTACSKAGITVAIEDIHSTYNFKSRNRS 51
+N+ K I + I + H T+N+ S NRS
Sbjct: 290 SNSTK*KGKICICIANTHWTFNYPSNNRS 204
>BP065061
Length = 302
Score = 25.0 bits (53), Expect = 3.7
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = -3
Query: 99 IYICNGHLAVYTSKLLRGC 117
+ ICNG + VY L RGC
Sbjct: 84 VLICNGAVPVYFCSLGRGC 28
>TC17880 similar to GB|AAN28894.1|23506069|AY143955 At2g28370/T1B3.11
{Arabidopsis thaliana;}, partial (34%)
Length = 540
Score = 24.6 bits (52), Expect = 4.8
Identities = 15/45 (33%), Positives = 23/45 (50%)
Frame = +3
Query: 26 ACSKAGITVAIEDIHSTYNFKSRNRSTGVAFSAALGLLECCFVNT 70
AC+ AGITV I++ + S ++ V F A G+ C+ T
Sbjct: 24 ACASAGITVLIDN-----DLGSCAKNHCVQFETATGMAFICWFTT 143
>TC15598 similar to UP|Q7XZI4 (Q7XZI4) DP1, partial (13%)
Length = 590
Score = 24.6 bits (52), Expect = 4.8
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = +1
Query: 12 YFLSTLHYTTWTNTAC 27
+ S L+Y TW NT+C
Sbjct: 517 FTFSRLNYCTWANTSC 564
>TC12063 similar to UP|HIS5_ARATH (Q9SZ30) Imidazole glycerol phosphate
synthase hisHF, chloroplast precursor (IGP synthase)
(ImGP synthase) (IGPS) [Includes: Glutamine
amidotransferase ; Cyclase ] , partial (11%)
Length = 582
Score = 24.6 bits (52), Expect = 4.8
Identities = 12/37 (32%), Positives = 19/37 (50%)
Frame = -1
Query: 86 DSDFWQSMRTHNDIYICNGHLAVYTSKLLRGCRCVCC 122
DSDF+ ++ +Y N HL + + RC+CC
Sbjct: 201 DSDFYAFLK-QMFLY*LNRHLFPMKNASSKRSRCICC 94
>AW719590
Length = 496
Score = 24.6 bits (52), Expect = 4.8
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +2
Query: 81 PGLVLDSDFWQSMRTHNDIYICNGHLAVYTSKLLR 115
PGL+ + F + + + GHLAVY +R
Sbjct: 80 PGLIRRASFSTTQASSKGFEVPKGHLAVYVGDEMR 184
>TC10261 homologue to UP|DAG_ANTMA (Q38732) DAG protein, chloroplast
precursor, partial (41%)
Length = 510
Score = 24.6 bits (52), Expect = 4.8
Identities = 17/57 (29%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = -3
Query: 60 LGLLECCFVNTGSRIMVFASGPGL-VLDSDFWQSMRTHNDIYICNGHLAVYTSKLLR 115
L L CCF+N+ + V G+ VL F S + + HL S++LR
Sbjct: 469 LEFLGCCFINSALKSSVGGGAKGIHVLLCFFHASKNSGESKEVSLNHLLT*RSRVLR 299
>TC14864 homologue to UP|AAN77867 (AAN77867)
5-enolpyruvylshikimate-3-phosphate synthase (Fragment),
partial (44%)
Length = 715
Score = 24.3 bits (51), Expect = 6.3
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +2
Query: 74 IMVFASGPGLVLDSDFWQSMRTHNDIYIC 102
+ +FA+GP + D W+ T I IC
Sbjct: 131 VALFANGPTAIRDVASWRVKETERMIAIC 217
>TC9890 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F13M22.5
{Arabidopsis thaliana;}, partial (6%)
Length = 547
Score = 24.3 bits (51), Expect = 6.3
Identities = 10/37 (27%), Positives = 18/37 (48%)
Frame = +3
Query: 66 CFVNTGSRIMVFASGPGLVLDSDFWQSMRTHNDIYIC 102
CFV+ ++F S P ++L FW+ + + C
Sbjct: 324 CFVSKSLFFIIFLSSPYVILFVIFWELLDGWKRSFTC 434
>TC15489 similar to UP|DDL_BACSU (P96612) D-alanine--D-alanine ligase
(D-alanylalanine synthetase) (D-Ala-D-Ala ligase) ,
partial (5%)
Length = 638
Score = 23.9 bits (50), Expect = 8.3
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = -3
Query: 43 YNFKSRNRSTGVAFSAALGLLEC 65
+NF S ++ + V+ + LGL EC
Sbjct: 135 WNFSSNDKISPVSLTVTLGLSEC 67
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.135 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,662,132
Number of Sequences: 28460
Number of extensions: 34565
Number of successful extensions: 274
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 272
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 273
length of query: 122
length of database: 4,897,600
effective HSP length: 79
effective length of query: 43
effective length of database: 2,649,260
effective search space: 113918180
effective search space used: 113918180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 49 (23.5 bits)
Medicago: description of AC146683.3