BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146683.16 + phase: 0
(1672 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB827612 124 9e-29
BP052828 107 1e-23
BG662087 102 5e-22
AV427422 74 2e-13
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 64 2e-10
AU089582 47 1e-09
BF177840 59 8e-09
BP065719 50 2e-06
BP055130 49 5e-06
AV410603 47 3e-05
TC18698 37 0.020
AV771315 30 2.4
TC14836 similar to UP|O24322 (O24322) Cysteine proteinase precur... 30 2.4
AV778124 29 5.3
TC13780 similar to UP|Q9SE42 (Q9SE42) D-ribulose-5-phosphate 3-e... 29 5.3
TC15695 similar to UP|Q93WX6 (Q93WX6) NIFS-like protein CpNifsp ... 29 5.3
>CB827612
Length = 277
Score = 124 bits (312), Expect = 9e-29
Identities = 57/92 (61%), Positives = 73/92 (78%)
Frame = +1
Query: 1528 SCHLPVELEHKAYWAIRNLNLDPNLAGDKRKLQLNELEELRMDAYENARIYKERTKTWHD 1587
+CHLPVE+EH+AYWA+++ N + AG +RKLQL +LEELR+ AYE++RIYKE+TK HD
Sbjct: 1 ACHLPVEIEHRAYWAVKSCNXEMQQAGIERKLQLQQLEELRLGAYESSRIYKEKTKHXHD 180
Query: 1588 KKIIKRHFKSGDLVLLFNSRLKLFPGKLRSRW 1619
K I ++ F G VLLFNSRLKL GKLRS+W
Sbjct: 181 KMISRKEFSVGQQVLLFNSRLKLMAGKLRSKW 276
>BP052828
Length = 523
Score = 107 bits (268), Expect = 1e-23
Identities = 57/148 (38%), Positives = 85/148 (56%), Gaps = 3/148 (2%)
Frame = +2
Query: 502 PCVIGSEVVKKAMCDLGASVSLMPLSLYERLGIGELKSTRMTLQLADRSVKYPAGIIEDV 561
PC IG + M DLGA +++MP S+Y L G L+ T + +QLA+RS P G++EDV
Sbjct: 71 PCTIGDNKYENCMLDLGAGINVMPTSIYNILHPGPLQHTGLIVQLANRSNARPKGVVEDV 250
Query: 562 PVKVGEVYIPADFVVMEME---EDNQVPILLGRPFLATAGAIIDVKKGKLAFNVGKETVE 618
V+V E+ PADF +++ME + ++ PI+LGRPF+ TA IDV G ++ G +
Sbjct: 251 LVQVNELIFPADFYILDMEGETKSSRAPIILGRPFMKTAKTKIDVDNGTMSMEFGDIIAK 430
Query: 619 FELAKLMKGPSIKDSYCMIDIIDHCVKE 646
F + MK P S I ++ V +
Sbjct: 431 FNIDDAMKHPLEDHSVFHIGVVSELVDD 514
>BG662087
Length = 373
Score = 102 bits (254), Expect = 5e-22
Identities = 47/109 (43%), Positives = 70/109 (64%)
Frame = +1
Query: 852 WRMCIDYRKLNKATRKDHFPLPFIDQMLERLAKHSHFCYLDGYSGFFQIPIHPNDQEKTT 911
WRM +DY LNKA KD +PLP ID++++ + + +D YSG+ QI +HP+D++KT
Sbjct: 22 WRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDKTA 201
Query: 912 FTCPFGTFAYRRMPFGLCNAPATFQRCMMSIFSDFVEKIMEVFMDDFSV 960
F + Y+ +PFGL NA AT+Q M +F D V + MEV++D+ V
Sbjct: 202 FMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIV 348
>AV427422
Length = 417
Score = 73.9 bits (180), Expect = 2e-13
Identities = 41/135 (30%), Positives = 72/135 (52%), Gaps = 1/135 (0%)
Frame = +1
Query: 1387 PSSFGNQYILVAVDYVSKWVEAIA-SPTNDAQVVIKMFKKVIFPRFGVPRVVISDGGSHF 1445
P S G + +LV VD +SK+ + A+V+ +F + + GVP ++SD F
Sbjct: 13 PKSKGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDPLF 192
Query: 1446 ISRHFEKLLQKLGVRHKIATPYHPQTSGQVEVSNRQIKAILEKTVSTSRTDWSNKLDDAL 1505
+S +++L + G + K++T YHP++ GQ EV NR ++ L ++ W++ + A
Sbjct: 193 MSNFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPWAE 372
Query: 1506 WAYRTAYKTPIGMTP 1520
+ Y T+Y G TP
Sbjct: 373 YWYNTSYHVSTGQTP 417
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 63.9 bits (154), Expect = 2e-10
Identities = 30/98 (30%), Positives = 54/98 (54%)
Frame = +2
Query: 1440 DGGSHFISRHFEKLLQKLGVRHKIATPYHPQTSGQVEVSNRQIKAILEKTVSTSRTDWSN 1499
+ G F S+ + +G++ + ++ HPQT+GQ E +N+ I +++ + + W +
Sbjct: 8 ENGIQFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWID 187
Query: 1500 KLDDALWAYRTAYKTPIGMTPFKLVYGKSCHLPVELEH 1537
+L LW+Y T ++ I TPF L YG LPVE+ +
Sbjct: 188 ELPIVLWSYNTMPQSSIKETPF*LTYGADTMLPVEISN 301
>AU089582
Length = 383
Score = 47.0 bits (110), Expect(2) = 1e-09
Identities = 26/74 (35%), Positives = 39/74 (52%)
Frame = +2
Query: 1421 KMFKKVIFPRFGVPRVVISDGGSHFISRHFEKLLQKLGVRHKIATPYHPQTSGQVEVSNR 1480
K++ I GVP +ISD G+ F S + LG R K++T +HPQT GQ E + +
Sbjct: 26 KIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQSERTIQ 205
Query: 1481 QIKAILEKTVSTSR 1494
++ +L V R
Sbjct: 206 ILEDMLRACVXDLR 247
Score = 34.3 bits (77), Expect(2) = 1e-09
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 1497 WSNKLDDALWAYRTAYKTPIGMTPFKLVYGKSCHLPV 1533
W L +AY +Y++ I M PF+ +YG+ C P+
Sbjct: 255 WDQYLSLMEFAYNNSYRSSI*MAPFEALYGRRCRSPI 365
>BF177840
Length = 410
Score = 58.5 bits (140), Expect = 8e-09
Identities = 30/99 (30%), Positives = 50/99 (50%)
Frame = +2
Query: 1437 VISDGGSHFISRHFEKLLQKLGVRHKIATPYHPQTSGQVEVSNRQIKAILEKTVSTSRTD 1496
++SD + FIS + L K+G + +T HPQT GQ EV N+ + +L + +
Sbjct: 17 IVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNLKM 196
Query: 1497 WSNKLDDALWAYRTAYKTPIGMTPFKLVYGKSCHLPVEL 1535
W L +AY + +PF++VYG + P++L
Sbjct: 197 WETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDL 313
>BP065719
Length = 567
Score = 50.4 bits (119), Expect = 2e-06
Identities = 35/119 (29%), Positives = 54/119 (44%)
Frame = +3
Query: 46 QFSGSPSEDPNLHISTFWRLSVTCKDDQETVRLYLFPFSLKDKASNWFNSLKPGSITSWD 105
+FSG E HI+ + ++ E +++ FP SL A WF +L P S+ +W
Sbjct: 195 KFSGDSGESTVEHIARY-QIEAGDLAINENLKMKYFPSSLTKNAFTWFTTLAPRSVHTWA 371
Query: 106 QLRREFLCRFFPPSKTAQLRGKLYQFTQKNEESLFDVWEHFKEILRRCPHHGLEKWLII 164
QL R F +FF + L +K ES+ D F+ + RC H E L++
Sbjct: 372 QLERIFHEQFFRGECKVSXK-DLASVKRKPAESIDDYLNRFRMLKSRCFTHVSEHELVV 545
>BP055130
Length = 567
Score = 49.3 bits (116), Expect = 5e-06
Identities = 32/112 (28%), Positives = 51/112 (44%), Gaps = 1/112 (0%)
Frame = +2
Query: 1391 GNQYILVAVDYVSKWVEAIASPTN-DAQVVIKMFKKVIFPRFGVPRVVISDGGSHFISRH 1449
G I+V VD +SK+ N + V + F I G+PR ++SD F S
Sbjct: 179 GFTVIIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAF 358
Query: 1450 FEKLLQKLGVRHKIATPYHPQTSGQVEVSNRQIKAILEKTVSTSRTDWSNKL 1501
++ + G +++ YHPQT GQ E N+ ++ L V + W + L
Sbjct: 359 WKHFFKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLWVSYL 514
>AV410603
Length = 162
Score = 46.6 bits (109), Expect = 3e-05
Identities = 22/54 (40%), Positives = 31/54 (56%)
Frame = +1
Query: 864 ATRKDHFPLPFIDQMLERLAKHSHFCYLDGYSGFFQIPIHPNDQEKTTFTCPFG 917
+T KD FP+P +D++L+ L F LD SG+ QI + P D+ KT F G
Sbjct: 1 STVKDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>TC18698
Length = 808
Score = 37.4 bits (85), Expect = 0.020
Identities = 21/57 (36%), Positives = 31/57 (53%)
Frame = -2
Query: 907 QEKTTFTCPFGTFAYRRMPFGLCNAPATFQRCMMSIFSDFVEKIMEVFMDDFSVHGS 963
++KTT + Y+ MP GL N T+QR M IF + K +EV+++D V S
Sbjct: 807 KKKTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKSS 637
>AV771315
Length = 511
Score = 30.4 bits (67), Expect = 2.4
Identities = 21/62 (33%), Positives = 26/62 (41%)
Frame = +1
Query: 353 NHPNKMNAVTLRSGKPLEDPIRRTETDNSEKEICEPPSRETRAEREKPRVEENLPPPFKP 412
N N + A LR G P T+ SEK+ + P ET +VE PP K
Sbjct: 109 NDLNNLKATELRLGLP--------GTEESEKKTKKRPLNETSEGSNAQQVESGSAPPSKA 264
Query: 413 KI 414
KI
Sbjct: 265 KI 270
>TC14836 similar to UP|O24322 (O24322) Cysteine proteinase precursor , partial
(92%)
Length = 1416
Score = 30.4 bits (67), Expect = 2.4
Identities = 10/21 (47%), Positives = 16/21 (75%)
Frame = +2
Query: 1525 YGKSCHLPVELEHKAYWAIRN 1545
YGK+ + P+ ++KAYW I+N
Sbjct: 953 YGKAGYAPIRFKNKAYWIIKN 1015
>AV778124
Length = 515
Score = 29.3 bits (64), Expect = 5.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -3
Query: 1264 NYQQKKKFFN-DLKHYYWDEPYLFRRGSDGIFRRC 1297
N +Q KF N + HYYWDE +L + S + C
Sbjct: 117 NEKQLFKFININTDHYYWDENHLMSKLSSSLMLIC 13
>TC13780 similar to UP|Q9SE42 (Q9SE42) D-ribulose-5-phosphate 3-epimerase ,
partial (50%)
Length = 445
Score = 29.3 bits (64), Expect = 5.3
Identities = 17/46 (36%), Positives = 20/46 (42%)
Frame = +2
Query: 117 PPSKTAQLRGKLYQFTQKNEESLFDVWEHFKEILRRCPHHGLEKWL 162
PP TA+ R L T K WE ++LRRC H WL
Sbjct: 32 PPPTTAKTRFYLLAVTNKR-------WE*HPKLLRRCSHRTSPIWL 148
>TC15695 similar to UP|Q93WX6 (Q93WX6) NIFS-like protein CpNifsp precursor
(At1g08490/T27G7_14) (Cysteine desulfurase) , partial
(27%)
Length = 602
Score = 29.3 bits (64), Expect = 5.3
Identities = 18/78 (23%), Positives = 38/78 (48%)
Frame = +3
Query: 198 LIENMALNLFQWTEEKATIDPSPSKKEAGMDETSSIDYLSTKVNALSQRLDRMSTPTFSP 257
++++ N +W ++ TI PSPS + E+ + L++ L + L +S P+ +
Sbjct: 177 ILQHFLTNSMEWLSDQVTIVPSPS---IAI*ESMQVHVLASISITLRRMLTTLSRPSMTQ 347
Query: 258 PCETCDLSSHSNTDCNLS 275
+ SS++ C L+
Sbjct: 348 *ASSIHSSSNNVFLCKLN 401
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,211,274
Number of Sequences: 28460
Number of extensions: 417343
Number of successful extensions: 2040
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 2011
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2038
length of query: 1672
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1569
effective length of database: 1,966,220
effective search space: 3084999180
effective search space used: 3084999180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146683.16