BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146683.12 - phase: 0
(480 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 37 0.009
AV773507 36 0.016
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 34 0.046
TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%) 32 0.23
TC17386 similar to UP|Q9LU46 (Q9LU46) DEAD-box protein abstrakt,... 32 0.30
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 31 0.51
TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, p... 31 0.51
AV421607 31 0.51
TC9100 similar to UP|Q8W544 (Q8W544) NADH-ubiquinone oxidoreduct... 30 0.67
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 30 0.87
TC8074 similar to UP|AAQ72789 (AAQ72789) 60S ribosomal protein L... 30 0.87
BP055887 29 1.5
TC14934 similar to UP|Q9LSN8 (Q9LSN8) Protein phosphatase 2C-lik... 29 1.5
AV420911 29 1.9
TC16587 weakly similar to UP|Q9JGS5 (Q9JGS5) PORF1, partial (3%) 29 1.9
BP039068 28 2.5
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 28 2.5
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 28 2.5
AV780703 28 2.5
BP053073 28 3.3
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 36.6 bits (83), Expect = 0.009
Identities = 16/45 (35%), Positives = 29/45 (63%), Gaps = 8/45 (17%)
Frame = +2
Query: 294 KKFLSKRGSYKNS--KKEDQKG------CFNCKKPGHFIADCPDL 330
+K S R SY+++ +++ ++G C NCK+PGHF +CP++
Sbjct: 260 RKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECPNV 394
Score = 31.2 bits (69), Expect = 0.39
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +2
Query: 314 CFNCKKPGHFIADCPD 329
C+NCK+PGH + CP+
Sbjct: 458 CWNCKEPGHMASSCPN 505
Score = 27.7 bits (60), Expect = 4.3
Identities = 21/87 (24%), Positives = 32/87 (36%), Gaps = 8/87 (9%)
Frame = +2
Query: 249 KTSKSSKAYKASESEEESPDGDSDEDQSVKMAMLSNKLEYLARKQKKFLSKRGSYKNSKK 308
K SS + S S SP D+ ++ S + R ++ S+ KN K+
Sbjct: 197 KRKMSSDSRSRSRSRSRSP-----MDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKR 361
Query: 309 EDQKG--------CFNCKKPGHFIADC 327
C NC PGH ++C
Sbjct: 362 PGHFARECPNVAICHNCGLPGHIASEC 442
>AV773507
Length = 496
Score = 35.8 bits (81), Expect = 0.016
Identities = 14/29 (48%), Positives = 16/29 (54%)
Frame = +2
Query: 300 RGSYKNSKKEDQKGCFNCKKPGHFIADCP 328
RGSY + Q CF C +PGH DCP
Sbjct: 308 RGSYGAGDRVGQDDCFECGRPGHRARDCP 394
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 34.3 bits (77), Expect = 0.046
Identities = 15/36 (41%), Positives = 20/36 (54%)
Frame = +2
Query: 303 YKNSKKEDQKGCFNCKKPGHFIADCPDLQKEKYKGK 338
Y S+ + CF C KPGHF +CP + E +GK
Sbjct: 386 YGGSRGSNGGECFKCGKPGHFARECPS-EGETRRGK 490
>TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%)
Length = 451
Score = 32.0 bits (71), Expect = 0.23
Identities = 20/54 (37%), Positives = 24/54 (44%)
Frame = -2
Query: 362 EDLDSESGSDKEEADDDAKAAMRLVATVSSEAVSEAESDSEDENEVYSKIPRQE 415
EDLDS+ D+E DDD EA EA DE+EV + P E
Sbjct: 189 EDLDSDGFRDEEIGDDDEGEGEE-----EDEATEEASGFDGDEHEVRERAPGDE 43
>TC17386 similar to UP|Q9LU46 (Q9LU46) DEAD-box protein abstrakt, partial
(19%)
Length = 507
Score = 31.6 bits (70), Expect = 0.30
Identities = 17/46 (36%), Positives = 23/46 (49%), Gaps = 2/46 (4%)
Frame = +2
Query: 312 KGCFNCKKPGHFIADCPDLQKEKYK--GKSKKSSFSSSKFRKQIKK 355
KGC C GH I DCP L+ +K ++ F S +R +I K
Sbjct: 203 KGCAYCGGLGHRIRDCPKLEHQKSMAIANNRHDYFGSGGYRGEI*K 340
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 30.8 bits (68), Expect = 0.51
Identities = 16/52 (30%), Positives = 25/52 (47%)
Frame = +1
Query: 300 RGSYKNSKKEDQKGCFNCKKPGHFIADCPDLQKEKYKGKSKKSSFSSSKFRK 351
RG + ED K C+ C +PGHF +C + G ++ S S + R+
Sbjct: 127 RGGGRGRGGEDLK-CYECGEPGHFARECRSRGGSRGLGSGRRRSPSPYRRRR 279
>TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, partial
(34%)
Length = 1827
Score = 30.8 bits (68), Expect = 0.51
Identities = 25/110 (22%), Positives = 49/110 (43%)
Frame = +1
Query: 354 KKSLMATWEDLDSESGSDKEEADDDAKAAMRLVATVSSEAVSEAESDSEDENEVYSKIPR 413
+ L A+ + L +S + A++ ++L + E+V ++S D +
Sbjct: 292 QSELEASQKHLSVDSAALASAANEIQLLKVQLELVANCESVQTHHAESAD-------VEL 450
Query: 414 QELVDSLKELLSLFEHRTNELTDLKEKYVDLMKQQKTTLLELKASEEELK 463
L +L E LSL E+ N+L + KE TLL+L+ ++ ++
Sbjct: 451 LNLKQNLSETLSLVENMKNQLRNFKEFEAQAQGLVSETLLQLETAKRTVE 600
>AV421607
Length = 245
Score = 30.8 bits (68), Expect = 0.51
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = +3
Query: 313 GCFNCKKPGHFIADCP 328
GC+ C +PGH+ DCP
Sbjct: 51 GCYKCGRPGHWSRDCP 98
>TC9100 similar to UP|Q8W544 (Q8W544) NADH-ubiquinone oxidoreductase
(Fragment), partial (97%)
Length = 712
Score = 30.4 bits (67), Expect = 0.67
Identities = 18/41 (43%), Positives = 23/41 (55%)
Frame = -3
Query: 421 KELLSLFEHRTNELTDLKEKYVDLMKQQKTTLLELKASEEE 461
+ LLSL TN L KEKY+ + KQQ + + E A E E
Sbjct: 644 ERLLSLKLLNTNNLLWRKEKYIQIEKQQSSNVAEDHAFEGE 522
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 30.0 bits (66), Expect = 0.87
Identities = 14/30 (46%), Positives = 15/30 (49%), Gaps = 5/30 (16%)
Frame = -2
Query: 314 CFNCKKPGHFIADCPDL-----QKEKYKGK 338
C C K GHF CPDL QK + GK
Sbjct: 329 CHRCSKKGHFANRCPDLVCWNCQKTGHSGK 240
Score = 26.9 bits (58), Expect = 7.4
Identities = 19/81 (23%), Positives = 40/81 (48%), Gaps = 2/81 (2%)
Frame = -2
Query: 298 SKRGSYKNSKKEDQKGCFNCKKPGHFIADC--PDLQKEKYKGKSKKSSFSSSKFRKQIKK 355
SK+G + N + C+NC+K GH DC P ++ +++ + +++K ++ +
Sbjct: 317 SKKGHFANRCPD--LVCWNCQKTGHSGKDCTNPKVEAATNAIAARRPAPAANKGKRPVAS 144
Query: 356 SLMATWEDLDSESGSDKEEAD 376
+ + T SG++ AD
Sbjct: 143 ARVYT------VSGAESHRAD 99
>TC8074 similar to UP|AAQ72789 (AAQ72789) 60S ribosomal protein L5, partial
(96%)
Length = 1086
Score = 30.0 bits (66), Expect = 0.87
Identities = 15/52 (28%), Positives = 31/52 (58%), Gaps = 1/52 (1%)
Frame = +3
Query: 328 PDLQKEKYKGKSKKSSFSSSKFRKQIKKS-LMATWEDLDSESGSDKEEADDD 378
P ++K + K K ++ K + +K+ L+A + L+S +G+D++E DD+
Sbjct: 744 PTIKKAEKKPPKKSKRYNLKKLTYEERKAKLIARLQALNSAAGADEDEEDDE 899
>BP055887
Length = 506
Score = 29.3 bits (64), Expect = 1.5
Identities = 17/41 (41%), Positives = 23/41 (55%)
Frame = +2
Query: 421 KELLSLFEHRTNELTDLKEKYVDLMKQQKTTLLELKASEEE 461
++ LSL TN L KEKY+ + KQQ + + E A E E
Sbjct: 44 RKTLSLKLLNTNNLLWRKEKYIQIEKQQSSNVAEDHAFEGE 166
>TC14934 similar to UP|Q9LSN8 (Q9LSN8) Protein phosphatase 2C-like protein,
partial (57%)
Length = 932
Score = 29.3 bits (64), Expect = 1.5
Identities = 18/69 (26%), Positives = 30/69 (43%)
Frame = +1
Query: 361 WEDLDSESGSDKEEADDDAKAAMRLVATVSSEAVSEAESDSEDENEVYSKIPRQELVDSL 420
WE L +E D ++ A +A RLV EA + E D ++ K+ R+ D +
Sbjct: 433 WEHLSNEQAVDIVHSNPRAGSAKRLVKAALQEAARKREMRYSDLRKIDKKV-RRHFHDDI 609
Query: 421 KELLSLFEH 429
++ H
Sbjct: 610 TVIVLFLNH 636
>AV420911
Length = 418
Score = 28.9 bits (63), Expect = 1.9
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = +2
Query: 300 RGSYKNSKKEDQKGCFNCKKPGHFIADC 327
RG + ED K C+ C +PGHF +C
Sbjct: 335 RGGGRGRGGEDLK-CYECGEPGHFAREC 415
>TC16587 weakly similar to UP|Q9JGS5 (Q9JGS5) PORF1, partial (3%)
Length = 706
Score = 28.9 bits (63), Expect = 1.9
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +1
Query: 231 NEHETSKKSKSIALPSKGKTSKSSKAYKASESEEESPD 268
N H TS S ++ALPS+ T+ ++ + S + +PD
Sbjct: 313 NSHSTSPHSSNLALPSEPLTALTTPKTPSPSSSKPAPD 426
>BP039068
Length = 467
Score = 28.5 bits (62), Expect = 2.5
Identities = 11/26 (42%), Positives = 13/26 (49%)
Frame = +3
Query: 305 NSKKEDQKGCFNCKKPGHFIADCPDL 330
N Q C NC++ GH DCP L
Sbjct: 285 NHNNPRQTVCMNCQETGHASNDCPSL 362
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 28.5 bits (62), Expect = 2.5
Identities = 12/38 (31%), Positives = 19/38 (49%)
Frame = +1
Query: 314 CFNCKKPGHFIADCPDLQKEKYKGKSKKSSFSSSKFRK 351
C+ C +PGHF +C G ++ S S +FR+
Sbjct: 379 CYECGEPGHFAREC-----RMRGGSGRRRSRSPPRFRR 477
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 28.5 bits (62), Expect = 2.5
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Frame = +2
Query: 314 CFNCKKPGHFIADCP--DLQKEKYKGKSKKSSFSSSKFRKQIKKSLMATWEDLDSESG 369
CFNC GH+ DC D + + Y+ + + KK+ TW++L S +G
Sbjct: 371 CFNCGLDGHWARDCKAGDWKNKCYRCGDR--GHVERNCKNSPKKNEWQTWKELFSFTG 538
>AV780703
Length = 454
Score = 28.5 bits (62), Expect = 2.5
Identities = 14/49 (28%), Positives = 27/49 (54%)
Frame = +3
Query: 246 SKGKTSKSSKAYKASESEEESPDGDSDEDQSVKMAMLSNKLEYLARKQK 294
+KG+ SK+ E+ ES +GD E V +++ N+L ++ ++ K
Sbjct: 135 TKGRLSKTEPKLGRLENTSESLEGDGRELTGVWLSIKKNRLGFIRKRGK 281
>BP053073
Length = 456
Score = 28.1 bits (61), Expect = 3.3
Identities = 12/35 (34%), Positives = 23/35 (65%)
Frame = +3
Query: 384 RLVATVSSEAVSEAESDSEDENEVYSKIPRQELVD 418
R+ + V S+ V+ + + ++DE E+Y I QE++D
Sbjct: 165 RVASAVFSQEVNNSVAFTQDEPEIYLPIKAQEIMD 269
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.308 0.126 0.340
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,338,610
Number of Sequences: 28460
Number of extensions: 70038
Number of successful extensions: 421
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 408
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 420
length of query: 480
length of database: 4,897,600
effective HSP length: 94
effective length of query: 386
effective length of database: 2,222,360
effective search space: 857830960
effective search space used: 857830960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146683.12