BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.4 - phase: 0
(320 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15257 similar to PIR|T39903|T39903 serine-rich protein - fissi... 80 3e-19
TC16546 85 2e-17
BP028625 38 0.002
CN825409 32 0.19
TC15930 similar to UP|O82520 (O82520) 3-oxoacyl-[acyl-carrier-pr... 31 0.24
TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPas... 30 0.42
TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like prot... 30 0.71
BP055890 29 1.2
TC16779 similar to UP|Q9LEY9 (Q9LEY9) Nhp2-like protein (AT5g081... 28 1.6
TC14821 homologue to UP|AAR12195 (AAR12195) Molecular chaperone ... 28 1.6
TC9649 similar to UP|Q95TP4 (Q95TP4) LD33277p, partial (4%) 28 1.6
BP070393 28 1.6
TC8423 similar to UP|AAQ62572 (AAQ62572) Glycosyltransferase 10,... 28 1.6
TC11276 weakly similar to UP|HDA1_CHICK (P56517) Histone deacety... 28 2.1
TC13825 similar to GB|AAH33913.1|21706954|BC033913 Paox protein ... 27 3.5
BI419099 27 4.6
TC10120 similar to UP|Q9LTA2 (Q9LTA2) Similarity to AT-hook DNA-... 27 4.6
TC9824 weakly similar to UP|Q84UV8 (Q84UV8) Nectarin III, partia... 27 4.6
TC19252 similar to UP|Q9FMS6 (Q9FMS6) CCR4-associated factor-lik... 27 4.6
>TC15257 similar to PIR|T39903|T39903 serine-rich protein - fission yeast
(Schizosaccharomyces pombe)
{Schizosaccharomyces pombe;}, partial (5%)
Length = 572
Score = 80.5 bits (197), Expect(2) = 3e-19
Identities = 48/136 (35%), Positives = 70/136 (51%), Gaps = 14/136 (10%)
Frame = +3
Query: 1 MDLAPEELQFLNIPNILKESISIPKISPKTFYLITLILIFPLSFAILAHSLFTHPLISHI 60
MD EE+QFL + + +ES I K F ITL LI PL+F +L H ++ L S I
Sbjct: 81 MDTEQEEMQFLGLFGVYRESYKIILSWRKIFSQITLTLILPLTFILLIHMEVSNILFSKI 260
Query: 61 ESPFTDPAQTSHD--------------WTLLLIIQFFYLIFLFAFSLLSTAAVVFTVASL 106
+ + T D W + + Y+ FL FSLL+T+AVV+TVAS+
Sbjct: 261 NNNTQEMLYTPQDTPQHKKLSDMISSEWATYWLFELAYITFLLIFSLLTTSAVVYTVASI 440
Query: 107 YTSKPVSFSNTISAIP 122
YT++ ++F +S P
Sbjct: 441 YTARDITFRGVLSVCP 488
Score = 30.8 bits (68), Expect(2) = 3e-19
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = +1
Query: 121 IPNVFKRLFITFLWVTLLMFCYNFVFI 147
+P V+KRL +TFL + +F YN V I
Sbjct: 484 VPRVWKRLMVTFLSTFVALFIYNLVAI 564
>TC16546
Length = 599
Score = 84.7 bits (208), Expect = 2e-17
Identities = 53/126 (42%), Positives = 75/126 (59%), Gaps = 2/126 (1%)
Frame = +1
Query: 197 EPLYGFAAMKKSYELLKGRVRYASLLVCGYLFLCAVISGMFSVIVVHGGD-GYDVFSRIF 255
E YGF AM KS EL+KG++ + L++ +I +F+ +VV G GY +
Sbjct: 1 EDSYGFKAMMKSNELMKGKMGLSILIILTLNVPSFLIYLLFNKLVVQGLRLGY--VEKTA 174
Query: 256 IGGFLVGVLVIVNLVGLLVQSVFYYVCKSYHHQEIDKSALHDHLGGYLGEYVPLKS-SIQ 314
G + +L + L G ++ SV Y+VCKSYHHQ IDKSAL DHL Y GEY PLK+ +Q
Sbjct: 175 YGILCLILLSHLILFGHVIHSVLYFVCKSYHHQNIDKSALSDHLEVYHGEYEPLKAQDVQ 354
Query: 315 MENLDI 320
+E+ +
Sbjct: 355 LEHYQV 372
>BP028625
Length = 566
Score = 38.1 bits (87), Expect = 0.002
Identities = 34/114 (29%), Positives = 55/114 (47%), Gaps = 5/114 (4%)
Frame = -1
Query: 178 VVHVYITALWHLASVVSVLEPLYGFAAMKKSYELLKGRVRYASLLVCGYLF--LCAVISG 235
VV VY+ A+ L VVSV E G+ A+ L++GR VCG++ L ++SG
Sbjct: 506 VVEVYLMAVMSLGIVVSVAEERLGWEAVTVGSGLMRGR------RVCGWVLSGLFVLVSG 345
Query: 236 MFSVIV---VHGGDGYDVFSRIFIGGFLVGVLVIVNLVGLLVQSVFYYVCKSYH 286
+ + V + G D V+ R L+ +V L+ ++ +VFY + H
Sbjct: 344 LINRRVEELLEGEDSISVWDRT----VLICWYGLVVLLSYVITTVFYCDSRKRH 195
>CN825409
Length = 739
Score = 31.6 bits (70), Expect = 0.19
Identities = 22/68 (32%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = -1
Query: 84 YLIFLFAFSLLSTAAVVFTVASLYTSKPVSFSNTISAIPNVFK-RLFITFLWVTLLMFCY 142
YL+ L A +L + + F AS ++ P+SFS+ S N FK ++ +++ +FC
Sbjct: 583 YLLCLTALNLSAFSFSSFLTASNSSNTPLSFSSRASYWSNAFKFGAVLSISDISVPIFCL 404
Query: 143 NFVFILCL 150
N IL L
Sbjct: 403 NSTSILSL 380
>TC15930 similar to UP|O82520 (O82520) 3-oxoacyl-[acyl-carrier-protein]
synthase , partial (16%)
Length = 695
Score = 31.2 bits (69), Expect = 0.24
Identities = 27/100 (27%), Positives = 37/100 (37%)
Frame = +3
Query: 59 HIESPFTDPAQTSHDWTLLLIIQFFYLIFLFAFSLLSTAAVVFTVASLYTSKPVSFSNTI 118
H SP A S W +L YL ++ ++F+V S S F
Sbjct: 279 H*HSP*RQEAA*S*CWCVL------YLFVPIVVTM*KLMFLIFSVLSAAFSNSFGFGGHN 440
Query: 119 SAIPNVFKRLFITFLWVTLLMFCYNFVFILCLVLMVIAVD 158
SAI N L ++ F Y F+F L L L + D
Sbjct: 441 SAISNYVHSFIYPMLLLSFHFFFYQFLFFLALFLCSLGRD 560
>TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPase subunit
RPT4a, partial (49%)
Length = 648
Score = 30.4 bits (67), Expect = 0.42
Identities = 17/59 (28%), Positives = 29/59 (48%)
Frame = -3
Query: 81 QFFYLIFLFAFSLLSTAAVVFTVASLYTSKPVSFSNTISAIPNVFKRLFITFLWVTLLM 139
QFF + F A + + + F + +P +F+N + PN KRL I F ++ L +
Sbjct: 337 QFFLIHFTAAANHIPGSTACFNNQAFIVKRPENFANYL---PNTLKRLQIIFSFIVLFL 170
>TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like protein, complete
Length = 1794
Score = 29.6 bits (65), Expect = 0.71
Identities = 26/101 (25%), Positives = 44/101 (42%), Gaps = 2/101 (1%)
Frame = -1
Query: 79 IIQFFYLIFLFA--FSLLSTAAVVFTVASLYTSKPVSFSNTISAIPNVFKRLFITFLWVT 136
++ F FLFA FS L ++F + L++ +P + + FL +
Sbjct: 1791 VLAFLLFSFLFAWHFS*LFRLCMLFPLFLLFSC----------IVPILQLNILSRFLPLF 1642
Query: 137 LLMFCYNFVFILCLVLMVIAVDTDNSVLLFFSVVLIFVLFL 177
LL +NF F L+ I + +LLF + L+ + FL
Sbjct: 1641 LLFLFHNFFF-----LITIFISQQRHILLFIFLFLLLLFFL 1534
>BP055890
Length = 542
Score = 28.9 bits (63), Expect = 1.2
Identities = 33/124 (26%), Positives = 49/124 (38%), Gaps = 7/124 (5%)
Frame = -3
Query: 33 LITLILIFPLSFAIL---AHSLFTHPLISHIESPFTDPAQTSHDWTLLLIIQFFYLIFLF 89
L+ LI+I PL F I+ HSL H + LLL+IQF + F
Sbjct: 483 LLLLIVILPLQFLIVHWNLHSLLIHAMF------------------LLLLIQFHQFLRQF 358
Query: 90 AFSL---LSTAAVVFTV-ASLYTSKPVSFSNTISAIPNVFKRLFITFLWVTLLMFCYNFV 145
SL +S + F+ SK +SF+ IT L + ++ F N
Sbjct: 357 LLSLF*YISYSTF*FSKDVKFQVSKVLSFTTAPYFKITSSSS*KITHLGLAVIPFASNIA 178
Query: 146 FILC 149
++ C
Sbjct: 177 YLYC 166
>TC16779 similar to UP|Q9LEY9 (Q9LEY9) Nhp2-like protein
(AT5g08180/T22D6_120), partial (17%)
Length = 850
Score = 28.5 bits (62), Expect = 1.6
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +1
Query: 128 LFITFLWVTLLMFCYNFVFILCLVLMVIAVDTDNSVLLFFS 168
LF+ FL+V + C+ F+LC L+ + D L S
Sbjct: 715 LFVLFLFVFFTLCCFPLCFLLC*TLISLGRDCSRDSLFIHS 837
>TC14821 homologue to UP|AAR12195 (AAR12195) Molecular chaperone Hsp90-1,
partial (29%)
Length = 612
Score = 28.5 bits (62), Expect = 1.6
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = -3
Query: 72 HDWTLLLIIQFFYLIFLFAFSLLSTAAVVFTVASLYTSKPVSFSNTIS 119
H W LI FF+ F F+ S ST +S +S +SFS S
Sbjct: 187 HSWDTSLIFFFFFSSFSFSSSTSSTLPSSSFFSSSSSSSEISFSVVFS 44
>TC9649 similar to UP|Q95TP4 (Q95TP4) LD33277p, partial (4%)
Length = 754
Score = 28.5 bits (62), Expect = 1.6
Identities = 13/42 (30%), Positives = 27/42 (63%), Gaps = 4/42 (9%)
Frame = +3
Query: 137 LLMFCYNFVFI----LCLVLMVIAVDTDNSVLLFFSVVLIFV 174
L++FC+NF F+ L ++L I ++ +S+ + ++V LI +
Sbjct: 474 LILFCFNFFFLF*RTL*IILSYITIERGSSL*ISYNVYLITI 599
>BP070393
Length = 359
Score = 28.5 bits (62), Expect = 1.6
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = -1
Query: 251 FSRIFIGGFLVGVLVIVNLVGLLVQSVFYYVC 282
++ + + LV +LV++NL+ LLV +FY C
Sbjct: 242 YTPLMMSTLLVSLLVVLNLICLLVLLLFYLQC 147
>TC8423 similar to UP|AAQ62572 (AAQ62572) Glycosyltransferase 10, partial
(35%)
Length = 1053
Score = 28.5 bits (62), Expect = 1.6
Identities = 29/109 (26%), Positives = 45/109 (40%), Gaps = 12/109 (11%)
Frame = +3
Query: 204 AMKKSYELLKGRVRYASLLVCGYLFLCAVISGMFSVIVVHGGDGYDVF------SRIFIG 257
A KK+ R+ Y L G+LFLC VHG Y ++ S +G
Sbjct: 420 ATKKTKSKFLERLNYLELAFAGFLFLCGCYD------YVHGKHNYFIYLFLQTLSFTIVG 581
Query: 258 GFLVGVLV---IVNLVGLLVQSVFYYVCKSYHHQ---EIDKSALHDHLG 300
VG +V + V +V VF ++ + HQ ++ ++ H H G
Sbjct: 582 VGYVGTIV*SSATSRVT*VVLIVF*FLLCNLLHQNLLQLVRADRHFHFG 728
>TC11276 weakly similar to UP|HDA1_CHICK (P56517) Histone deacetylase 1
(HD1), partial (5%)
Length = 636
Score = 28.1 bits (61), Expect = 2.1
Identities = 27/110 (24%), Positives = 49/110 (44%), Gaps = 2/110 (1%)
Frame = -2
Query: 82 FFYLIFLFAFSLLSTAAVVFTVASLYTSKPVSFSNTISAIP--NVFKRLFITFLWVTLLM 139
+ L +F + ++ ++F ++ V IS P N ++ FL++ +
Sbjct: 344 YICLSLMFPLTNHTSIRILFHALNMVICGHVGIILPISLDPL*NRVNLSWLLFLFLLFFL 165
Query: 140 FCYNFVFILCLVLMVIAVDTDNSVLLFFSVVLIFVLFLVVHVYITALWHL 189
F + F L L L+ + L FF++ LIF+LFL + + LW L
Sbjct: 164 FIFLLFFFLGLFLLRLFF------LGFFTLWLIFLLFLFLGFFF--LWFL 39
Score = 26.2 bits (56), Expect = 7.9
Identities = 25/69 (36%), Positives = 33/69 (47%), Gaps = 9/69 (13%)
Frame = -2
Query: 34 ITLILIFPL----SFAILAHSLFTHPLISHIES--PFT-DPA--QTSHDWTLLLIIQFFY 84
I L L+FPL S IL H+L + H+ P + DP + + W L L + FF
Sbjct: 341 ICLSLMFPLTNHTSIRILFHAL-NMVICGHVGIILPISLDPL*NRVNLSWLLFLFLLFFL 165
Query: 85 LIFLFAFSL 93
IFL F L
Sbjct: 164 FIFLLFFFL 138
>TC13825 similar to GB|AAH33913.1|21706954|BC033913 Paox protein {Mus
musculus;} , partial (25%)
Length = 451
Score = 27.3 bits (59), Expect = 3.5
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 74 WTLLLIIQFFYLIFLFAF 91
W L L+I FF+L F+F+F
Sbjct: 213 WDLKLVILFFFLSFIFSF 266
>BI419099
Length = 586
Score = 26.9 bits (58), Expect = 4.6
Identities = 13/46 (28%), Positives = 22/46 (47%)
Frame = +1
Query: 264 LVIVNLVGLLVQSVFYYVCKSYHHQEIDKSALHDHLGGYLGEYVPL 309
L ++ GL F+ VCK +++ SA +G +G+Y L
Sbjct: 418 LGVLGFSGLSAYGGFFEVCKPQSGEKVFVSAASGSIGNLVGQYAKL 555
>TC10120 similar to UP|Q9LTA2 (Q9LTA2) Similarity to AT-hook DNA-binding
protein, partial (40%)
Length = 847
Score = 26.9 bits (58), Expect = 4.6
Identities = 11/37 (29%), Positives = 23/37 (61%)
Frame = +1
Query: 163 VLLFFSVVLIFVLFLVVHVYITALWHLASVVSVLEPL 199
+LLF+ F+ F + Y++ + ++ASV+ ++E L
Sbjct: 595 ILLFYLFAFFFLSFFGLFNYLSLVIYIASVIRLIETL 705
>TC9824 weakly similar to UP|Q84UV8 (Q84UV8) Nectarin III, partial (42%)
Length = 973
Score = 26.9 bits (58), Expect = 4.6
Identities = 22/83 (26%), Positives = 39/83 (46%), Gaps = 4/83 (4%)
Frame = -3
Query: 5 PEELQFLNIPNILKESISIPKISPKTFYLITLILIFPLSFAILAHSLFTH----PLISHI 60
P L + +PN++ +SIPK ++ L+ PL+F +L+ S + P++ HI
Sbjct: 686 PTYL*YFLLPNLMSPGLSIPK---------SMSLLSPLNF-MLSSSFESKGSGWPIVYHI 537
Query: 61 ESPFTDPAQTSHDWTLLLIIQFF 83
P T + WT + + F
Sbjct: 536 --PITALSPLEFSWTACISMSNF 474
>TC19252 similar to UP|Q9FMS6 (Q9FMS6) CCR4-associated factor-like protein,
partial (50%)
Length = 629
Score = 26.9 bits (58), Expect = 4.6
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 7/53 (13%)
Frame = +2
Query: 134 WVTLLMFCYNFVFILCLVLMVIAV-------DTDNSVLLFFSVVLIFVLFLVV 179
W+ F ++ +LCL MV+ + D D +V LFF VLI+ + V+
Sbjct: 470 WIFFSSFPIVYLLVLCL*SMVLGLIFMNVL*D*DATVRLFFKDVLIYGVETVI 628
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.332 0.146 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,255,996
Number of Sequences: 28460
Number of extensions: 102575
Number of successful extensions: 1203
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1198
length of query: 320
length of database: 4,897,600
effective HSP length: 90
effective length of query: 230
effective length of database: 2,336,200
effective search space: 537326000
effective search space used: 537326000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146651.4