BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.2 - phase: 0
(384 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18788 weakly similar to UP|Q9SSA6 (Q9SSA6) F4P13.1 protein, pa... 61 3e-10
BI420292 48 3e-06
BP033618 47 7e-06
TC8119 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding protei... 37 0.007
TC8603 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protei... 36 0.012
TC12890 homologue to UP|Q40359 (Q40359) Alfin-1, partial (23%) 34 0.036
AV410494 34 0.036
TC8118 similar to GB|AAN28771.1|23505823|AY143832 At3g11200/F11B... 34 0.047
TC10171 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding prote... 33 0.080
TC11597 33 0.10
BP031190 33 0.10
TC17191 homologue to UP|Q9SL38 (Q9SL38) At2g18090 protein, parti... 32 0.14
TC18764 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prote... 32 0.14
TC15130 similar to UP|O81488 (O81488) F9D12.13 protein, partial ... 30 0.52
BP073977 28 2.0
TC15336 28 2.0
BP060014 28 2.0
AV768577 28 3.3
TC14667 homologue to UP|Q84P43 (Q84P43) Protein kinase Pti1, com... 27 4.4
TC15846 similar to UP|Q8UV28 (Q8UV28) P32INGL, partial (16%) 27 5.7
>TC18788 weakly similar to UP|Q9SSA6 (Q9SSA6) F4P13.1 protein, partial (7%)
Length = 951
Score = 61.2 bits (147), Expect = 3e-10
Identities = 32/87 (36%), Positives = 45/87 (50%), Gaps = 3/87 (3%)
Frame = +1
Query: 84 CVICDLG---GNLLCCDSCPRTYHLQCLDPPLKRIPMGKWQCPSCFEENDQLKPLNNLDS 140
C +C L ++L CD+C YH CL+PPL RIP G W CPSC + + +
Sbjct: 115 CKVCGLDKDDDSVLLCDTCDAEYHTYCLNPPLARIPEGNWYCPSCVDGKHATQNVIEHSQ 294
Query: 141 ISRRARTKTVPVKSKAGVNPVNLEKVS 167
I + R+K K VN +NLE ++
Sbjct: 295 IIGKRRSK----KFLGEVNRLNLEALT 363
>BI420292
Length = 553
Score = 47.8 bits (112), Expect = 3e-06
Identities = 22/55 (40%), Positives = 30/55 (54%), Gaps = 2/55 (3%)
Frame = +2
Query: 84 CVICDLGGNLLCCD--SCPRTYHLQCLDPPLKRIPMGKWQCPSCFEENDQLKPLN 136
C +C GG+L+ CD C R +HL+C P RIP G W C C E + K ++
Sbjct: 134 CSLCSNGGDLISCDRDKCTRAFHLKCARLP--RIPSGLWYCKYCENETQENKDVD 292
Score = 36.2 bits (82), Expect = 0.009
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 13/55 (23%)
Frame = +2
Query: 83 ECVIC-------DLGG--NLLCCDSCPRTYHLQCL----DPPLKRIPMGKWQCPS 124
+C +C D+ G ++ CD C + YH++CL L+++P GKW C S
Sbjct: 332 QCALCGGHDFIKDVFGPRTVMICDQCEKEYHVECLKDHNKQNLEKLPEGKWFCCS 496
>BP033618
Length = 467
Score = 46.6 bits (109), Expect = 7e-06
Identities = 20/47 (42%), Positives = 25/47 (52%), Gaps = 2/47 (4%)
Frame = +2
Query: 84 CVICDLGGNLL-CCDS-CPRTYHLQCLDPPLKRIPMGKWQCPSCFEE 128
C C GG L+ CC C R+YH C+DPP +P W C C E+
Sbjct: 326 CCKCSRGGMLMRCCGKRCQRSYHPSCVDPPFNYVPPXFWYCIQCVEK 466
>TC8119 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding protein-like,
partial (85%)
Length = 1249
Score = 36.6 bits (83), Expect = 0.007
Identities = 28/97 (28%), Positives = 43/97 (43%), Gaps = 13/97 (13%)
Frame = +1
Query: 42 KEDNSVASESSRSASAKRMLKTEEGTAQFSSKKKGHDGY------FYECVICDLGGNL-- 93
K+ +V S S KR + +G + S+ K +GY E + GGN
Sbjct: 661 KDKPTVDSGSKSRGGTKR---SSDGQVKSSNPKFADEGYEEDEDEHSETLCGSCGGNYNA 831
Query: 94 ----LCCDSCPRTYHLQCLD-PPLKRIPMGKWQCPSC 125
+ CD C R YH +C+ P K + +++CPSC
Sbjct: 832 DEFWIGCDVCERWYHGKCVKITPAKAESIKQYKCPSC 942
>TC8603 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (88%)
Length = 1232
Score = 35.8 bits (81), Expect = 0.012
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 19/103 (18%)
Frame = +1
Query: 42 KEDNSVASES---SRSASAKRMLKTEEGTAQFSSKKKGHDGYFYE---------CVICDL 89
KE +SV++ S S+S+S R +T+ ++ S K +G + C C
Sbjct: 565 KEKSSVSNNSGSKSKSSSKARAAETQGRQSKVSLSKDEDEGIEEQDDDEHGDALCGAC-- 738
Query: 90 GGNL------LCCDSCPRTYHLQCLD-PPLKRIPMGKWQCPSC 125
G N +CCD C + +H +C+ P + + +++CPSC
Sbjct: 739 GDNYGTDEFWICCDICEKWFHGKCVKITPARAEHIKQYKCPSC 867
>TC12890 homologue to UP|Q40359 (Q40359) Alfin-1, partial (23%)
Length = 460
Score = 34.3 bits (77), Expect = 0.036
Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +3
Query: 94 LCCDSCPRTYHLQCLD-PPLKRIPMGKWQCPSC 125
+CCD C R +H +C+ P K + +++CPSC
Sbjct: 96 ICCDMCERWFHGKCVKITPAKAEHIKQYKCPSC 194
>AV410494
Length = 398
Score = 34.3 bits (77), Expect = 0.036
Identities = 18/43 (41%), Positives = 22/43 (50%), Gaps = 4/43 (9%)
Frame = +2
Query: 84 CVICDLGGNLLCCD--SCPRTYHLQCL--DPPLKRIPMGKWQC 122
C IC GG+L+ CD CP+ YH C+ D R KW C
Sbjct: 152 CFICFDGGSLVLCDRRGCPKAYHPSCVKRDEAFFR-SKAKWNC 277
>TC8118 similar to GB|AAN28771.1|23505823|AY143832 At3g11200/F11B9.12
{Arabidopsis thaliana;}, partial (89%)
Length = 1177
Score = 33.9 bits (76), Expect = 0.047
Identities = 30/104 (28%), Positives = 46/104 (43%), Gaps = 15/104 (14%)
Frame = +2
Query: 37 EQSNGKEDNSVASESSRSASAKRMLKTEEGTAQFSSKKKGHDGYFYE--------CVICD 88
++ K+ + S S S KR + +G + S+ K +GY E C C
Sbjct: 569 DRKPAKDKPATDSGSKSRGSTKR---SSDGQVK-SNPKFAEEGYEEEDDEHSETLCGSC- 733
Query: 89 LGGNL------LCCDSCPRTYHLQCLD-PPLKRIPMGKWQCPSC 125
GGN + CD C R YH +C+ P K + +++CPSC
Sbjct: 734 -GGNYNADEFWIGCDICERWYHGKCVKITPAKAESIKQYKCPSC 862
>TC10171 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding protein-like,
partial (26%)
Length = 506
Score = 33.1 bits (74), Expect = 0.080
Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +2
Query: 94 LCCDSCPRTYHLQCLD-PPLKRIPMGKWQCPSC 125
+CCD C + +H +C+ P + + ++QCPSC
Sbjct: 92 ICCDICEKWFHGKCVKITPARAEHIKQYQCPSC 190
>TC11597
Length = 586
Score = 32.7 bits (73), Expect = 0.10
Identities = 20/60 (33%), Positives = 30/60 (49%), Gaps = 1/60 (1%)
Frame = +2
Query: 309 ANQKKRRTEKGKKVVITPIKSKSGNNKVQTKQKSKTHKISISASK-GDVGKKKSDAQQKD 367
A +KK+R +KG TP+KS + +TK ++ K SK GD+ KK + D
Sbjct: 125 AFEKKQRKQKGLWTGGTPVKSSKPSTSTKTKTETLVRKQLPQGSKNGDIKAKKKIVSESD 304
>BP031190
Length = 488
Score = 32.7 bits (73), Expect = 0.10
Identities = 14/38 (36%), Positives = 17/38 (43%), Gaps = 5/38 (13%)
Frame = -2
Query: 93 LLCCD-----SCPRTYHLQCLDPPLKRIPMGKWQCPSC 125
L+C D SC H+ C DPP + W CP C
Sbjct: 322 LICGDESGSLSCGIGTHIDCCDPPFPEVTEEDWFCPKC 209
>TC17191 homologue to UP|Q9SL38 (Q9SL38) At2g18090 protein, partial (5%)
Length = 598
Score = 32.3 bits (72), Expect = 0.14
Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 2/27 (7%)
Frame = -2
Query: 84 CVICDLGGNLLCCD--SCPRTYHLQCL 108
C IC GG+L+ CD CP+ YH C+
Sbjct: 114 CFICFDGGSLVLCDRRGCPKAYHPACV 34
>TC18764 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (35%)
Length = 848
Score = 32.3 bits (72), Expect = 0.14
Identities = 13/43 (30%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = +1
Query: 94 LCCDSCPRTYHLQCLD-PPLKRIPMGKWQCPSCFEENDQLKPL 135
+CCD C + +H +C+ P + + ++CPSC + + PL
Sbjct: 298 ICCDICEKWFHGKCVKITPARAEHIKPYKCPSCSHQRARS*PL 426
>TC15130 similar to UP|O81488 (O81488) F9D12.13 protein, partial (26%)
Length = 583
Score = 30.4 bits (67), Expect = 0.52
Identities = 18/89 (20%), Positives = 41/89 (45%), Gaps = 8/89 (8%)
Frame = +3
Query: 45 NSVASESSRSASAKRMLKTEEGTAQFSSKKKGHDGYFYECVICDLGGN-------LLCCD 97
+++AS +++++S ++ + Q + + D + +C G +CCD
Sbjct: 9 SNIASNNTKTSSKAGGSESVKYLKQVKDEDEEEDDEEHGETLCGACGENYASDEFWICCD 188
Query: 98 SCPRTYHLQCLD-PPLKRIPMGKWQCPSC 125
C +H +C+ P + + ++CPSC
Sbjct: 189 ICENWFHGKCVKITPARAEHIKHYKCPSC 275
>BP073977
Length = 424
Score = 28.5 bits (62), Expect = 2.0
Identities = 14/27 (51%), Positives = 19/27 (69%)
Frame = +2
Query: 326 PIKSKSGNNKVQTKQKSKTHKISISAS 352
PI KS +NK++T QKSK S+S+S
Sbjct: 170 PIMVKSEHNKIKTGQKSKPVSSSLSSS 250
>TC15336
Length = 1145
Score = 28.5 bits (62), Expect = 2.0
Identities = 20/74 (27%), Positives = 34/74 (45%), Gaps = 5/74 (6%)
Frame = +2
Query: 184 KSISTMGGKFFGMKPVLSPVDATCSDKPMDPSLESCMEG-TSCADADEKNLNLSPTVAPM 242
+++S + G F PVL P D +DP ++ + SC A+ K + L+ + + +
Sbjct: 260 EAVSGVDGVFHTASPVLVPYDDNIQATLIDPCIKGALNVLNSCVKANVKRVVLTSSCSSI 439
Query: 243 ----DRMSVSPDKE 252
D VSP E
Sbjct: 440 RYRDDEQQVSPLNE 481
>BP060014
Length = 366
Score = 28.5 bits (62), Expect = 2.0
Identities = 13/27 (48%), Positives = 15/27 (55%), Gaps = 2/27 (7%)
Frame = +3
Query: 83 ECVICDLGGNLLCC--DSCPRTYHLQC 107
+C C L G L C SC RTYH+ C
Sbjct: 243 KCSTCGLKGAALGCYVKSCKRTYHVPC 323
>AV768577
Length = 497
Score = 27.7 bits (60), Expect = 3.3
Identities = 14/44 (31%), Positives = 22/44 (49%)
Frame = -1
Query: 310 NQKKRRTEKGKKVVITPIKSKSGNNKVQTKQKSKTHKISISASK 353
NQ K+ + K T + + G VQT K KTHK ++ ++
Sbjct: 386 NQTKQVQNRAPKRNQT*LTRRDGTKNVQTGSKQKTHKPKVAKTE 255
>TC14667 homologue to UP|Q84P43 (Q84P43) Protein kinase Pti1, complete
Length = 1591
Score = 27.3 bits (59), Expect = 4.4
Identities = 17/59 (28%), Positives = 24/59 (39%), Gaps = 7/59 (11%)
Frame = +2
Query: 113 KRIPMGKWQCPSC-------FEENDQLKPLNNLDSISRRARTKTVPVKSKAGVNPVNLE 164
KR M +W C +C END LK N + + + PVK + P +E
Sbjct: 137 KRTRMRRWLCCTCQVEEPYPSNENDHLKSPRNYGDGNSKGSKASAPVKHETQKAPPPIE 313
>TC15846 similar to UP|Q8UV28 (Q8UV28) P32INGL, partial (16%)
Length = 639
Score = 26.9 bits (58), Expect = 5.7
Identities = 12/39 (30%), Positives = 20/39 (50%), Gaps = 4/39 (10%)
Frame = +1
Query: 91 GNLLCCDS--CP--RTYHLQCLDPPLKRIPMGKWQCPSC 125
G+++ CD+ C +H C+ + GKW CP+C
Sbjct: 91 GDMIACDNENCQGGEWFHYACVGLTQETRFKGKWYCPTC 207
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.129 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,030,822
Number of Sequences: 28460
Number of extensions: 83308
Number of successful extensions: 370
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 368
length of query: 384
length of database: 4,897,600
effective HSP length: 92
effective length of query: 292
effective length of database: 2,279,280
effective search space: 665549760
effective search space used: 665549760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146651.2