BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.18 + phase: 0 /pseudo
(559 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP038017 58 5e-09
TC11884 56 2e-08
TC16246 weakly similar to UP|Q8S2M2 (Q8S2M2) Far-red impaired re... 40 0.001
AV425218 38 0.004
AW164060 35 0.025
TC12114 weakly similar to GB|BAC16510.1|23237937|AP005198 transp... 34 0.055
BP038217 32 0.27
CB828260 31 0.46
TC19180 similar to PIR|T10255|T10255 nitrite transport protein, ... 31 0.46
BP046936 31 0.60
TC12072 30 0.79
AV419295 30 1.0
BP053360 30 1.3
AU251819 30 1.3
CN825057 29 1.8
BP041860 29 2.3
TC10485 29 2.3
TC11785 homologue to UP|Q9ZVC2 (Q9ZVC2) At2g41370 protein, parti... 28 3.0
BI419972 28 3.0
TC14261 weakly similar to UP|Q05929 (Q05929) EDGP precursor (Fra... 28 3.9
>BP038017
Length = 570
Score = 57.8 bits (138), Expect = 5e-09
Identities = 36/148 (24%), Positives = 64/148 (42%), Gaps = 1/148 (0%)
Frame = +2
Query: 25 YNERQQIRKANRGDKKSLQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNF 84
Y + Q R R + L Y ++ YY+ + + ++FWA +N F
Sbjct: 131 YVKSSQKRTLGRDAQNLLNYFKKMQGKNPGFYYAIQLDDENRMINVFWADARSRSAYNYF 310
Query: 85 PTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTS 144
++ D+ Y+ N Y++ GV G + E E +F W + R L++
Sbjct: 311 GDAVIFDTMYRPNQYQVPFAPFTGVNHHGQNVLFGCALLLDESESSFTW---LFRTWLSA 481
Query: 145 KMNMPKV-IVTDRDMSLMKAVENIFPKS 171
+ P V I TD+D ++ AV +FP++
Sbjct: 482 MNDRPPVSITTDQDRAIQAAVAQVFPET 565
>TC11884
Length = 557
Score = 55.8 bits (133), Expect = 2e-08
Identities = 37/110 (33%), Positives = 54/110 (48%), Gaps = 1/110 (0%)
Frame = +1
Query: 271 HLGCRTSNMVESAHALLKKYLDNSVGDLGTFWEKIHDMLLLQFTAIQTTFGQSVTVLEHR 330
H G TSN E AHA LKK L + GDL T W+ H + + T I +F +S+ ++H
Sbjct: 1 HFGTTTSNRAEGAHASLKKMLRDCKGDLATSWDASHSLTCNRHTEILASFERSIHRIDHI 180
Query: 331 FKDVILYSDLGGHVSRYALDNSALEETRCRE-TLCIENNIVQRTSYGLPC 379
F Y+++ G VS L E R + C + + R ++ LPC
Sbjct: 181 FM-CPFYTNIRGFVSNKCLQLLDAEHIRMKSYGRC---DCLLRETHELPC 318
>TC16246 weakly similar to UP|Q8S2M2 (Q8S2M2) Far-red impaired response
protein-like, partial (4%)
Length = 660
Score = 40.0 bits (92), Expect = 0.001
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = +2
Query: 41 SLQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMY 99
+L YL K + +Y T++ ++E++FW +N F V+ DSTYK N Y
Sbjct: 161 ALSYLEGKADNDPMFFYKFTKTGDESLENLFWCDGVSRMDYNVFGDVIAFDSTYKKNKY 337
>AV425218
Length = 419
Score = 38.1 bits (87), Expect = 0.004
Identities = 20/82 (24%), Positives = 40/82 (48%)
Frame = +2
Query: 106 VVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTSKMNMPKVIVTDRDMSLMKAVE 165
+VGV + G ++ E E+FVW+ + L ++ P+ I+TD ++ KA+E
Sbjct: 17 LVGVNHHGQSVLFGCALLSSEDSESFVWLFQSLLHCMSGV--PPQGIITDHSEAMKKAIE 190
Query: 166 NIFPKSYTMNCYFHVQANVKQR 187
+ P + C ++ + Q+
Sbjct: 191 TVLPSTRHRWCLSYIMKKLPQK 256
>AW164060
Length = 406
Score = 35.4 bits (80), Expect = 0.025
Identities = 25/69 (36%), Positives = 36/69 (51%), Gaps = 12/69 (17%)
Frame = +3
Query: 434 EVKEGLRQLTFSETTM--MSPPPRKVP-TKGAKKKV------DIARLLIPKIRIVN--RH 482
E + G + TFSE T+ ++PPP T KK+V D+ L+PK+ + H
Sbjct: 171 ESQPGTPKYTFSENTLPPLTPPPSYYSSTSPCKKQVKKRSRSDLLLALLPKLNLKKTFSH 350
Query: 483 HHQQ-HHHI 490
HHQ HHH+
Sbjct: 351 HHQPLHHHL 377
>TC12114 weakly similar to GB|BAC16510.1|23237937|AP005198 transposase-like
{Oryza sativa (japonica cultivar-group);}, partial (8%)
Length = 488
Score = 34.3 bits (77), Expect = 0.055
Identities = 21/95 (22%), Positives = 40/95 (42%)
Frame = +1
Query: 42 LQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMYKM 101
LQY KL E++ Y++ + I ++FW + + F ++ +DSTY T+
Sbjct: 58 LQYFQRKLVENSPFYHAYQLDDEDQITNVFWVDARMLIDYGYFGDMVSLDSTYCTHSSNR 237
Query: 102 LMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLK 136
+ G G + E E++ W+ +
Sbjct: 238 PLAVFSGFNHHRKAVIFGAALLYDETTESY*WLFE 342
>BP038217
Length = 514
Score = 32.0 bits (71), Expect = 0.27
Identities = 20/74 (27%), Positives = 32/74 (43%)
Frame = +1
Query: 206 PRDVVKKIMRA*KAMVELPTQELYANASVEFQNSCSDFSLFKNYAMTTLNEVKEKIVRAW 265
P +V+ K+ K ELP +E +E +S + Y EV K + W
Sbjct: 268 PSEVIAKLQAVGKEFFELPQEEKEVYGKIEGSDS------LEGYGTKLQKEVNGK--KGW 423
Query: 266 TDHVLHLGCRTSNM 279
DH+ H+ TS++
Sbjct: 424 VDHLFHIIWPTSSI 465
>CB828260
Length = 557
Score = 31.2 bits (69), Expect = 0.46
Identities = 12/36 (33%), Positives = 24/36 (66%)
Frame = +3
Query: 1 MLPKNILIHLKDKRPHCMTNVKQVYNERQQIRKANR 36
+LP+ +L+ LK+ P +T + Q+Y +++RK+ R
Sbjct: 450 VLPRKMLLTLKENNPLNLTTISQIYGACKRLRKSLR 557
>TC19180 similar to PIR|T10255|T10255 nitrite transport protein, chloroplast
- cucumber {Cucumis sativus;} , partial (12%)
Length = 408
Score = 31.2 bits (69), Expect = 0.46
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 475 KIRIVNRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYPKP 515
++ + HHH HHH RL + SL+P SP +P
Sbjct: 213 RLHVCFHHHHHAHHH-------RLVRPSLDPCSPQVHRARP 314
>BP046936
Length = 557
Score = 30.8 bits (68), Expect = 0.60
Identities = 21/95 (22%), Positives = 40/95 (42%), Gaps = 1/95 (1%)
Frame = +1
Query: 452 PPPRKVPTKGAKKKVDIARLLIPKIRIVNRHHHQQHHHIKEKKDV-RLGKTSLNPLSPPF 510
PPP + P+ + +A L+ ++ HH ++ ++ + +T L+PP
Sbjct: 79 PPPPRHPSSSSFTLSTMASSLLSSCMSLSHHHSRRRRNLLSNSQLTSFNRTPTTNLNPPP 258
Query: 511 RYPKPKTILVMRPIDYMSRYMLPFIEKMVDVIGDG 545
R K + + + +R P E+ V V G G
Sbjct: 259 RSGKVMAVALQPAQEVTTRRKPPVKERRVVVTGLG 363
>TC12072
Length = 532
Score = 30.4 bits (67), Expect = 0.79
Identities = 15/46 (32%), Positives = 24/46 (51%)
Frame = -3
Query: 479 VNRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYPKPKTILVMRPI 524
+NR + + + K+ LG+TSLNP P+ K + L RP+
Sbjct: 173 INRLSIRFNQQTQTKEHYALGQTSLNPAQAPYNTSKHQPCLSRRPL 36
>AV419295
Length = 383
Score = 30.0 bits (66), Expect = 1.0
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +1
Query: 452 PPPRKVPTKGA-KKKVDIARLLIPKIRIVNRHH-HQQHHHIKEKKDVRLG 499
PPP K+PT +++ +R+L+P I + HH + HH K + R G
Sbjct: 181 PPPTKLPTPTP*EERGSRSRILLPLITFITSHHLPRTCHHFKPTRRRRGG 330
>BP053360
Length = 530
Score = 29.6 bits (65), Expect = 1.3
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = -3
Query: 482 HHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYP 513
HHHQ++HH++ V L + +N S P
Sbjct: 108 HHHQKYHHLRSPSLVNLNRRYINSSSSEVESP 13
>AU251819
Length = 368
Score = 29.6 bits (65), Expect = 1.3
Identities = 15/43 (34%), Positives = 21/43 (47%), Gaps = 5/43 (11%)
Frame = +2
Query: 452 PPPRKVPTKGA-----KKKVDIARLLIPKIRIVNRHHHQQHHH 489
PPP P+ G + + R+ IP + N HHH+ HHH
Sbjct: 62 PPPLPPPSSGMAMVCRRHP*KLIRIHIP---LKNHHHHRHHHH 181
>CN825057
Length = 721
Score = 29.3 bits (64), Expect = 1.8
Identities = 20/84 (23%), Positives = 39/84 (45%), Gaps = 1/84 (1%)
Frame = +1
Query: 27 ERQQIRKANRGDKK-SLQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFP 85
++ Q + GD + L+Y +E+ +YS +E + +IFW + + +F
Sbjct: 445 KKGQYLAMDEGDAQVMLEYFKHIQKENPNFFYSIDLNEEQRLRNIFWIDAKSINDYLSFN 624
Query: 86 TVLVMDSTYKTNMYKMLMFEVVGV 109
V+ D++Y + K+ VGV
Sbjct: 625 DVVSFDTSYIKSNEKLPFAPFVGV 696
>BP041860
Length = 362
Score = 28.9 bits (63), Expect = 2.3
Identities = 20/50 (40%), Positives = 24/50 (48%)
Frame = -3
Query: 447 TTMMSPPPRKVPTKGAKKKVDIARLLIPKIRIVNRHHHQQHHHIKEKKDV 496
T + S P K+P K A+ + L IP I I HHI EKKDV
Sbjct: 285 TKIASVSPTKIPIKTARPRTT-RNLSIPIILITEN----MLHHITEKKDV 151
>TC10485
Length = 636
Score = 28.9 bits (63), Expect = 2.3
Identities = 15/43 (34%), Positives = 22/43 (50%)
Frame = +3
Query: 452 PPPRKVPTKGAKKKVDIARLLIPKIRIVNRHHHQQHHHIKEKK 494
PPP+ VP+ KV++++ + HHH HHH K K
Sbjct: 159 PPPQFVPSS-KNSKVNLSK------KHKTLHHHHHHHH*KTHK 266
>TC11785 homologue to UP|Q9ZVC2 (Q9ZVC2) At2g41370 protein, partial (42%)
Length = 644
Score = 28.5 bits (62), Expect = 3.0
Identities = 23/80 (28%), Positives = 36/80 (44%), Gaps = 8/80 (10%)
Frame = +1
Query: 418 KAIVEHIKKLLFQMKLEVKEGLRQL--TFSETTMMSPPPRKVPTKGAK----KKVDIARL 471
KA +E + K+L + K+ + QL T S S P +V K K++ RL
Sbjct: 253 KASIEDVMKVLLASR---KQDMHQLWTTCSHLVAKSGLPPEVLAKHLPIDIVAKIEELRL 423
Query: 472 --LIPKIRIVNRHHHQQHHH 489
+ + ++ HHH HHH
Sbjct: 424 KSTLARRSLIPHHHHHHHHH 483
>BI419972
Length = 498
Score = 28.5 bits (62), Expect = 3.0
Identities = 15/47 (31%), Positives = 21/47 (43%), Gaps = 5/47 (10%)
Frame = -3
Query: 472 LIPKIR-----IVNRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYP 513
L+P +R + + HHH+ HHH+ L P P F YP
Sbjct: 451 LLPHLRHLSSPLPHHHHHRLHHHLHH----------LRPHHPLFHYP 341
>TC14261 weakly similar to UP|Q05929 (Q05929) EDGP precursor (Fragment),
partial (84%)
Length = 1604
Score = 28.1 bits (61), Expect = 3.9
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +3
Query: 471 LLIPKIRIVNRH---HHQQHHHIKEKKDVRLGKTSLNPL 506
LLIP+ R+ +H H QHHH + + SLNP+
Sbjct: 351 LLIPQARLQQQHLWPHPGQHHHRNRHQRRARPRHSLNPV 467
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.139 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,526,531
Number of Sequences: 28460
Number of extensions: 129796
Number of successful extensions: 1870
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 1402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1653
length of query: 559
length of database: 4,897,600
effective HSP length: 95
effective length of query: 464
effective length of database: 2,193,900
effective search space: 1017969600
effective search space used: 1017969600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146651.18